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Protein

Probable pectinesterase/pectinesterase inhibitor 41

Gene

PME41

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Acts in the modification of cell walls via demethylesterification of cell wall pectin.By similarity

Catalytic activityi

Pectin + n H2O = n methanol + pectate.

Pathwayi: pectin degradation

This protein is involved in step 1 of the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Probable pectinesterase/pectinesterase inhibitor 7 (PME7), Pectinesterase (AXX17_At1g11890), Pectinesterase (AXX17_At4g02940), Pectinesterase (AXX17_At5g52320), Probable pectinesterase/pectinesterase inhibitor 42 (PME42), Probable pectinesterase 15 (PME15), Putative pectinesterase 14 (PME14), Pectinesterase (At3g10720), Pectinesterase (AXX17_At2g33410), Probable pectinesterase 55 (PME55), Probable pectinesterase/pectinesterase inhibitor 23 (PME23), Pectinesterase (AXX17_At4g18720), Pectinesterase 4 (PME4), Putative pectinesterase/pectinesterase inhibitor 22 (PME22), Pectinesterase (AXX17_At1g48290), Pectinesterase (AXX17_At5g47900), Pectinesterase (At3g14310), Pectinesterase (AXX17_At4g38050), Pectinesterase (AXX17_At5g07100), Pectinesterase/pectinesterase inhibitor 18 (PME18), Pectinesterase (AXX17_At2g14510), Pectinesterase (AXX17_At3g43380), Pectinesterase (AXX17_At5g64270), Probable pectinesterase/pectinesterase inhibitor 64 (PME64), Putative pectinesterase 10 (PME10), Pectinesterase (F14I3.7), Probable pectinesterase 29 (PME29), Pectinesterase, Pectinesterase (AXX17_At4g38040), Probable pectinesterase 8 (PME8), Pectinesterase (AXX17_At5g27870), Pectinesterase (AXX17_At5g18820), Pectinesterase (AXX17_At2g45340), Pectinesterase 31 (PME31), Pectinesterase (AXX17_At3g41380), Pectinesterase (AXX17_At1g11650), Probable pectinesterase/pectinesterase inhibitor 25 (PME25), Probable pectinesterase/pectinesterase inhibitor 51 (PME51), Pectinesterase (AXX17_At3g36460), Pectinesterase (AXX17_At5g04430), Pectinesterase (At3g62170), Probable pectinesterase/pectinesterase inhibitor 20 (PME20), Pectinesterase (T27B3.30), Probable pectinesterase/pectinesterase inhibitor 60 (PME60), Probable pectinesterase/pectinesterase inhibitor 59 (PME59), Pectinesterase QRT1 (QRT1), Pectinesterase (AXX17_At3g53530), Pectinesterase (AXX17_At5g61190), Pectinesterase PPME1 (PPME1), Pectinesterase (AXX17_At3g56460), Pectinesterase (AXX17_At3g06710), Probable pectinesterase/pectinesterase inhibitor 32 (PME32), Pectinesterase (AXX17_At1g44540), Probable pectinesterase/pectinesterase inhibitor 33 (PME33), Probable pectinesterase/pectinesterase inhibitor 36 (PME36), Probable pectinesterase/pectinesterase inhibitor 13 (PME13), Putative pectinesterase 52 (PME52), Probable pectinesterase/pectinesterase inhibitor 54 (PME54), Pectinesterase (AXX17_At2g33420), Pectinesterase (AXX17_At2g40510), Pectinesterase (At1g53840), Pectinesterase 5 (PME5), Probable pectinesterase/pectinesterase inhibitor 16 (PME16), Probable pectinesterase 30 (PME30), Probable pectinesterase/pectinesterase inhibitor VGDH2 (VGDH2), Pectinesterase (AXX17_At3g26070), Probable pectinesterase 68 (PME68), Pectinesterase, Pectinesterase (AXX17_At3g14900), Pectinesterase (AXX17_At5g54740), Probable pectinesterase 67 (PME67), Putative pectinesterase/pectinesterase inhibitor 38 (PME38), Probable pectinesterase 56 (PME56), Pectinesterase, Probable pectinesterase/pectinesterase inhibitor 47 (PME47), Putative pectinesterase/pectinesterase inhibitor 28 (PME28), Probable pectinesterase 53 (PME53), Probable pectinesterase/pectinesterase inhibitor 46 (PME46), Pectinesterase (AXX17_At5g07090), Probable pectinesterase/pectinesterase inhibitor 17 (PME17), Pectinesterase (AXX17_At2g22270), Probable pectinesterase/pectinesterase inhibitor 61 (PME61), Probable pectinesterase 66 (PME66), Pectinesterase (AXX17_At2g42790), Pectinesterase (AXX17_At5g45990), Probable pectinesterase/pectinesterase inhibitor 6 (PME6), Probable pectinesterase 48 (PME48), Probable pectinesterase 49 (PME49), Pectinesterase (AXX17_At1g64100), Probable pectinesterase 50 (PME50), Pectinesterase (AXX17_At3g17940), Pectinesterase, Probable pectinesterase/pectinesterase inhibitor 19 (PME19), Pectinesterase (AXX17_At2g45050), Probable pectinesterase/pectinesterase inhibitor 40 (PME40), Pectinesterase (AXX17_At1g11920), Pectinesterase (AXX17_At3g30550), PME26 (AXX17_At3g14890), Probable pectinesterase/pectinesterase inhibitor 39 (PME39), Pectinesterase (AXX17_At2g44790), Pectinesterase (AXX17_At2g17140), Pectinesterase (AXX17_At5g19650), Putative pectinesterase/pectinesterase inhibitor 43 (PME43), Pectinesterase (AXX17_At3g31890), Pectinesterase (AXX17_At4g02900), Probable pectinesterase/pectinesterase inhibitor 34 (PME34), Pectinesterase 1 (PME1), Putative pectinesterase 63 (PME63), Pectinesterase (AXX17_At3g05180), Pectinesterase 2 (PME2), Probable pectinesterase/pectinesterase inhibitor 21 (PME21), Pectinesterase (AXX17_At2g22280), Pectinesterase (AXX17_At1g51200), Putative pectinesterase/pectinesterase inhibitor 45 (PME45), Probable pectinesterase/pectinesterase inhibitor 12 (PME12), Pectinesterase (AXX17_At4g02930), Probable pectinesterase/pectinesterase inhibitor 44 (PME44), Pectinesterase, Pectinesterase/pectinesterase inhibitor 3 (PME3), Pectinesterase (AXX17_At1g24390), Pectinesterase (AXX17_At5g50300), Pectinesterase (AXX17_At1g11900), Probable pectinesterase/pectinesterase inhibitor 58 (PME58), Putative pectinesterase 57 (PME57), Pectinesterase (AXX17_At3g10560), Pectinesterase (AXX17_At3g55110), Pectinesterase (AXX17_At3g10570), Putative pectinesterase 11 (PME11), Pectinesterase (AXX17_At1g01970), Pectinesterase (AXX17_At5g07110), Pectinesterase (AXX17_At4g06570), Pectinesterase (AXX17_At5g50310), Pectinesterase, Pectinesterase (AXX17_At1g51190), Pectinesterase (AXX17_At2g33420), Pectinesterase (AXX17_At1g48300), Pectinesterase (AXX17_At3g05190), Putative pectinesterase/pectinesterase inhibitor 24 (PME24), Putative pectinesterase/pectinesterase inhibitor 26 (PME26), Probable pectinesterase/pectinesterase inhibitor 35 (PME35), Pectinesterase (AXX17_At5g04420), Pectinesterase (AXX17_At2g44780), Pectinesterase (AXX17_At4g00230), Pectinesterase (AXX17_At5g09310), Pectinesterase (AXX17_At5g20800), Pectinesterase (AXX17_At1g04730), Probable pectinesterase/pectinesterase inhibitor 41 (PME41), Pectinesterase (AXX17_At1g11660), Pectinesterase (AXX17_At5g26780)
  2. Pectate lyase (AXX17_At3g26720), Probable pectate lyase 7 (At3g01270), Probable pectate lyase 18 (At4g24780), Probable pectate lyase 16 (At4g22080), Putative pectate lyase 17 (At4g22090), Pectate lyase (AXX17_At3g09180), Putative pectate lyase 14 (At4g13210), Pectate lyase (At3g55140), Pectate lyase (AXX17_At5g54880), Pectate lyase (AXX17_At4g15660), Probable pectate lyase 22 (At5g63180), Pectate lyase (T5E8_80), Pectate lyase (AXX17_At3g00310), Pectate lyase (At3g07010), Probable pectate lyase 8 (At3g07010), Probable pectate lyase 4 (At1g30350), Pectate lyase (AXX17_At2g01680), Putative pectate lyase 21 (At5g55720), Pectate lyase (At4g13210), Pectate lyase (At5g04310), Pectate lyase (AXX17_At5g03700), Pectate lyase (At4g13710), Pectate lyase (AXX17_At3g49720), Pectate lyase (At3g01270), Pectate lyase (At5g04310), Pectate lyase (AXX17_At1g12300), Probable pectate lyase 9 (At3g24230), Probable pectate lyase 1 (At1g04680), Pectate lyase (AXX17_At5g08800), Pectate lyase (T26I12.20), Pectate lyase (AXX17_At5g62760), Pectate lyase (AXX17_At3g47590), Pectate lyase (AXX17_At4g14900), Pectate lyase (AXX17_At3g49450), Pectate lyase (AXX17_At4g25600), Pectate lyase (AXX17_At1g61710), Probable pectate lyase 20 (At5g48900), Pectate lyase (AXX17_At5g47570), Probable pectate lyase 6 (At2g02720), Pectate lyase (AXX17_At3g06910), Pectate lyase (At1g14420), Pectate lyase (At3g01270), Pectate lyase (At2g02720), Pectate lyase (AXX17_At1g04020), Pectate lyase (At3g01270), Pectate lyase (At3g55140), Putative pectate lyase 2 (At1g11920), Pectate lyase, Pectate lyase (AXX17_At1g30780), Probable pectate lyase 19 (At5g15110), Pectate lyase (F11F8_12), Probable pectate lyase 13 (PMR6), Probable pectate lyase 3 (AT59), Pectate lyase (AXX17_At3g26200), Probable pectate lyase 5 (At1g67750), Probable pectate lyase 12 (At3g53190), Pectate lyase (At4g24780), Pectate lyase (AXX17_At4g25610), Probable pectate lyase 15 (At4g13710), Pectate lyase (At3g53190), Putative pectate lyase 11 (At3g27400), Pectate lyase (AXX17_At1g15010), Pectate lyase (At3g07010), Pectate lyase (AXX17_At3g29900), Pectate lyase (AXX17_At5g14600), Probable pectate lyase 10 (At3g24670)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
This subpathway is part of the pathway pectin degradation, which is itself part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin, the pathway pectin degradation and in Glycan metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei336Substrate; for pectinesterase activityBy similarity1
Binding sitei366Substrate; for pectinesterase activityBy similarity1
Sitei388Transition state stabilizerBy similarity1
Active sitei389Proton donor; for pectinesterase activityPROSITE-ProRule annotation1
Active sitei410Nucleophile; for pectinesterase activityPROSITE-ProRule annotation1
Binding sitei478Substrate; for pectinesterase activityBy similarity1
Binding sitei480Substrate; for pectinesterase activityBy similarity1

GO - Molecular functioni

  • aspartyl esterase activity Source: UniProtKB-KW
  • pectinesterase activity Source: TAIR
  • pectinesterase inhibitor activity Source: GO_Central

GO - Biological processi

  • cell wall modification Source: InterPro
  • pectin catabolic process Source: UniProtKB-UniPathway
  • response to brassinosteroid Source: TAIR
  • response to cold Source: TAIR
  • response to fungus Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Aspartyl esterase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciARA:AT4G02330-MONOMER.
UniPathwayiUPA00545; UER00823.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable pectinesterase/pectinesterase inhibitor 41
Including the following 2 domains:
Pectinesterase inhibitor 41
Alternative name(s):
Pectin methylesterase inhibitor 41
Pectinesterase 41 (EC:3.1.1.11)
Short name:
PE 41
Alternative name(s):
AtPMEpcrB
Pectin methylesterase 41
Short name:
AtPME41
Gene namesi
Name:PME41
Synonyms:ARATH41
Ordered Locus Names:At4g02330
ORF Names:T14P8.14
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G02330.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000037169123 – 573Probable pectinesterase/pectinesterase inhibitor 41Add BLAST551

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi29N-linked (GlcNAc...)Sequence analysis1
Glycosylationi119N-linked (GlcNAc...)Sequence analysis1
Glycosylationi173N-linked (GlcNAc...)Sequence analysis1
Glycosylationi264N-linked (GlcNAc...)Sequence analysis1
Glycosylationi268N-linked (GlcNAc...)Sequence analysis1
Glycosylationi281N-linked (GlcNAc...)Sequence analysis1
Glycosylationi320N-linked (GlcNAc...)Sequence analysis1
Glycosylationi353N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi403 ↔ 423By similarity
Glycosylationi456N-linked (GlcNAc...)Sequence analysis1
Glycosylationi469N-linked (GlcNAc...)Sequence analysis1
Glycosylationi520N-linked (GlcNAc...)Sequence analysis1
Glycosylationi541N-linked (GlcNAc...)Sequence analysis1
Glycosylationi547N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8RXK7.

Expressioni

Tissue specificityi

Expressed in flowers, siliques, floral stems and rosettes leaves.2 Publications

Developmental stagei

Expressed throughout silique development.1 Publication

Gene expression databases

GenevisibleiQ8RXK7. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G02330.1.

Structurei

3D structure databases

ProteinModelPortaliQ8RXK7.
SMRiQ8RXK7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni25 – 184Pectinesterase inhibitor 41Add BLAST160
Regioni259 – 557Pectinesterase 41Add BLAST299

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi192 – 195Poly-Lys4

Sequence similaritiesi

In the N-terminal section; belongs to the PMEI family.Curated
In the C-terminal section; belongs to the pectinesterase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IFP7. Eukaryota.
COG4677. LUCA.
HOGENOMiHOG000217409.
InParanoidiQ8RXK7.
KOiK01051.
OMAiPKNTHSH.
OrthoDBiEOG0936078I.
PhylomeDBiQ8RXK7.

Family and domain databases

Gene3Di1.20.140.40. 1 hit.
2.160.20.10. 1 hit.
InterProiIPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
IPR033131. Pectinesterase_Asp_AS.
IPR000070. Pectinesterase_cat.
IPR006501. Pectinesterase_inhib_dom.
[Graphical view]
PfamiPF01095. Pectinesterase. 1 hit.
PF04043. PMEI. 1 hit.
[Graphical view]
SMARTiSM00856. PMEI. 1 hit.
[Graphical view]
SUPFAMiSSF101148. SSF101148. 1 hit.
SSF51126. SSF51126. 1 hit.
TIGRFAMsiTIGR01614. PME_inhib. 1 hit.
PROSITEiPS00503. PECTINESTERASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8RXK7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSLKLFLVT LFLSLQTLFI ASQTLLPSNS SSTICKTTPD PKFCKSVFPQ
60 70 80 90 100
TSQGDVREYG RFSLRKSLTQ SRKFTRTIDR YLKRNNALLS QSAVGALQDC
110 120 130 140 150
RYLASLTTDY LITSFETVNI TTSSKTLSFS KADEIQTLLS AALTNEQTCL
160 170 180 190 200
DGINTAASSS WTIRNGVALP LINDTKLFSV SLALFTKGWV PKKKKQVASY
210 220 230 240 250
SWAHPKNTHS HTKPFRHFRN GALPLKMTEH TRAVYESLSR RKLADDDNDV
260 270 280 290 300
NTVLVSDIVT VNQNGTGNFT TITEAVNSAP NKTDGTAGYF VIYVTSGVYE
310 320 330 340 350
ENVVIAKNKR YLMMIGDGIN RTVVTGNRNV VDGWTTFNSA TFAVTSPNFV
360 370 380 390 400
AVNMTFRNTA GPEKHQAVAM RSSADLSIFY SCSFEAYQDT LYTHSLRQFY
410 420 430 440 450
RECDIYGTVD FIFGNAAVVF QDCNLYPRQP MQNQFNAITA QGRTDPNQNT
460 470 480 490 500
GISIHNCTIK PADDLVSSNY TVKTYLGRPW KEYSRTVFMQ SYIDEVVEPV
510 520 530 540 550
GWREWNGDFA LSTLYYAEYN NTGSGSSTTD RVVWPGYHVI NSTDANNFTV
560 570
ENFLLGDGWM VQSGVPYISG LLS
Length:573
Mass (Da):63,944
Last modified:May 5, 2009 - v2
Checksum:i863625D6142BB587
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti446P → Q in AAL87311 (PubMed:14593172).Curated1
Sequence conflicti455H → Y in AAC02974 (PubMed:9767082).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF069298 Genomic DNA. Translation: AAC19280.1.
AL161494 Genomic DNA. Translation: CAB80726.1.
CP002687 Genomic DNA. Translation: AEE82156.1.
AY150433 mRNA. Translation: AAN12975.1.
AY080836 mRNA. Translation: AAL87311.1.
AF033206 Genomic DNA. Translation: AAC02974.1.
PIRiT01317.
RefSeqiNP_567227.1. NM_116466.4.
UniGeneiAt.43016.

Genome annotation databases

EnsemblPlantsiAT4G02330.1; AT4G02330.1; AT4G02330.
GeneIDi828064.
GrameneiAT4G02330.1; AT4G02330.1; AT4G02330.
KEGGiath:AT4G02330.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF069298 Genomic DNA. Translation: AAC19280.1.
AL161494 Genomic DNA. Translation: CAB80726.1.
CP002687 Genomic DNA. Translation: AEE82156.1.
AY150433 mRNA. Translation: AAN12975.1.
AY080836 mRNA. Translation: AAL87311.1.
AF033206 Genomic DNA. Translation: AAC02974.1.
PIRiT01317.
RefSeqiNP_567227.1. NM_116466.4.
UniGeneiAt.43016.

3D structure databases

ProteinModelPortaliQ8RXK7.
SMRiQ8RXK7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT4G02330.1.

Proteomic databases

PaxDbiQ8RXK7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G02330.1; AT4G02330.1; AT4G02330.
GeneIDi828064.
GrameneiAT4G02330.1; AT4G02330.1; AT4G02330.
KEGGiath:AT4G02330.

Organism-specific databases

TAIRiAT4G02330.

Phylogenomic databases

eggNOGiENOG410IFP7. Eukaryota.
COG4677. LUCA.
HOGENOMiHOG000217409.
InParanoidiQ8RXK7.
KOiK01051.
OMAiPKNTHSH.
OrthoDBiEOG0936078I.
PhylomeDBiQ8RXK7.

Enzyme and pathway databases

UniPathwayiUPA00545; UER00823.
BioCyciARA:AT4G02330-MONOMER.

Miscellaneous databases

PROiQ8RXK7.

Gene expression databases

GenevisibleiQ8RXK7. AT.

Family and domain databases

Gene3Di1.20.140.40. 1 hit.
2.160.20.10. 1 hit.
InterProiIPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
IPR033131. Pectinesterase_Asp_AS.
IPR000070. Pectinesterase_cat.
IPR006501. Pectinesterase_inhib_dom.
[Graphical view]
PfamiPF01095. Pectinesterase. 1 hit.
PF04043. PMEI. 1 hit.
[Graphical view]
SMARTiSM00856. PMEI. 1 hit.
[Graphical view]
SUPFAMiSSF101148. SSF101148. 1 hit.
SSF51126. SSF51126. 1 hit.
TIGRFAMsiTIGR01614. PME_inhib. 1 hit.
PROSITEiPS00503. PECTINESTERASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPME41_ARATH
AccessioniPrimary (citable) accession number: Q8RXK7
Secondary accession number(s): O49008, O81300
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: May 5, 2009
Last modified: November 30, 2016
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The PMEI region may act as an autoinhibitory domain and prevent untimely PME activity during transport.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.