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Protein

Probable splicing factor 3A subunit 1

Gene

At1g14650

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei196Critical for binding to SF3A3By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Names & Taxonomyi

Protein namesi
Recommended name:
Probable splicing factor 3A subunit 1
Gene namesi
Ordered Locus Names:At1g14650
ORF Names:T5E21.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G14650.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001149191 – 785Probable splicing factor 3A subunit 1Add BLAST785

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ8RXF1.
PRIDEiQ8RXF1.

PTM databases

iPTMnetiQ8RXF1.

Expressioni

Gene expression databases

GenevisibleiQ8RXF1. AT.

Interactioni

Subunit structurei

Component of splicing factor SF3A which is composed of three subunits.By similarity

Protein-protein interaction databases

IntActiQ8RXF1. 1 interactor.
STRINGi3702.AT1G14650.1.

Structurei

Secondary structure

1785
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi686 – 688Combined sources3
Helixi692 – 698Combined sources7
Beta strandi703 – 707Combined sources5
Beta strandi713 – 715Combined sources3
Beta strandi718 – 723Combined sources6
Beta strandi725 – 728Combined sources4
Helixi729 – 739Combined sources11
Turni744 – 746Combined sources3
Beta strandi747 – 750Combined sources4
Beta strandi752 – 755Combined sources4
Turni762 – 766Combined sources5
Beta strandi768 – 770Combined sources3
Beta strandi772 – 776Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WE6NMR-A683-780[»]
ProteinModelPortaliQ8RXF1.
SMRiQ8RXF1.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8RXF1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati71 – 113SURP motif 1Add BLAST43
Repeati193 – 235SURP motif 2Add BLAST43
Domaini707 – 782Ubiquitin-likePROSITE-ProRule annotationAdd BLAST76

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi287 – 386Glu-richAdd BLAST100
Compositional biasi536 – 677Pro-richAdd BLAST142
Compositional biasi588 – 660Met-richAdd BLAST73

Domaini

SURP motif 2 mediates direct binding to SF3A3.By similarity

Sequence similaritiesi

Contains 2 SURP motif repeats.PROSITE-ProRule annotation
Contains 1 ubiquitin-like domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0007. Eukaryota.
ENOG410XPNW. LUCA.
HOGENOMiHOG000238941.
InParanoidiQ8RXF1.
KOiK12825.
OMAiGPARINV.
OrthoDBiEOG093605KI.
PhylomeDBiQ8RXF1.

Family and domain databases

InterProiIPR022030. SF3A1.
IPR000061. Surp.
IPR029071. Ubiquitin-rel_dom.
IPR000626. Ubiquitin_dom.
[Graphical view]
PfamiPF12230. PRP21_like_P. 1 hit.
PF01805. Surp. 2 hits.
PF00240. ubiquitin. 1 hit.
[Graphical view]
SMARTiSM00648. SWAP. 2 hits.
SM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF109905. SSF109905. 2 hits.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50128. SURP. 2 hits.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8RXF1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFSSMQILPL EAPPTDGKLG PLPPSQLTDQ EVEERELQAE QNNSNLAPPA
60 70 80 90 100
AVATHTRTIG IIHPPPDIRT IVEKTAQFVS KNGLEFEKRI IVSNEKNAKF
110 120 130 140 150
NFLKSSDPYH AFYQHKLTEY RAQNKDGAQG TDDSDGTTDP QLDTGAADES
160 170 180 190 200
EAGDTQPDLQ AQFRIPSKPL EAPEPEKYTV RLPEGITGEE LDIIKLTAQF
210 220 230 240 250
VARNGKSFLT GLSNRENNNP QFHFMKPTHS MFTFFTSLVD AYSEVLMPPK
260 270 280 290 300
DLKEKLRKSA ADLTTVLERC LHRLEWDRSQ EQQKKKEEDE KELERVQMAM
310 320 330 340 350
IDWHDFVVVE SIDFADEEDE ELPPPMTLDE VIRRSKASAM EEDEIVEPGK
360 370 380 390 400
EVEMEMDEEE VKLVAEGMRA ANLEENVKIE NVHDEEAPMR IVKNWKRPED
410 420 430 440 450
RIPTERDPTK VVISPITGEL IPINEMSEHM RISLIDPKFK EQKDRMFAKI
460 470 480 490 500
RETTLAQDDE IAKNIVGLAR LRPDIFGTTE EEVSNAVKAE IEKKKDEQPK
510 520 530 540 550
QVIWDGHTGS IGRTANQALS QNANGEEQGD GVYGDPNSFP GPAALPPPRP
560 570 580 590 600
GVPIVRPLPP PPNLALNLPR PPPSAQYPGA PRPLGVPMMQ PMHQQHQLTM
610 620 630 640 650
PGPPGHPQMM MNRPPQMQPG MHVPPPPGSQ FAHHMQIPRP YGQLPPSAMG
660 670 680 690 700
MMQPPPMPGM APPPPPEEAP PPLPEEPEAK RQKFDESALV PEDQFLAQHP
710 720 730 740 750
GPATIRVSKP NENDGQFMEI TVQSLSENVG SLKEKIAGEI QIPANKQKLS
760 770 780
GKAGFLKDNM SLAHYNVGAG EILTLSLRER GGRKR
Length:785
Mass (Da):87,594
Last modified:June 12, 2007 - v2
Checksum:i917E388B77472F8D
GO

Sequence cautioni

The sequence AAF63169 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti289D → G in AAL91182 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC010657 Genomic DNA. Translation: AAF63169.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE29196.1.
CP002684 Genomic DNA. Translation: AEE29197.1.
AY081293 mRNA. Translation: AAL91182.1.
AK226440 mRNA. Translation: BAE98583.1.
PIRiG86280.
RefSeqiNP_001117289.1. NM_001123817.1.
NP_172917.1. NM_101332.4.
UniGeneiAt.22146.

Genome annotation databases

EnsemblPlantsiAT1G14650.1; AT1G14650.1; AT1G14650.
AT1G14650.2; AT1G14650.2; AT1G14650.
GeneIDi838027.
GrameneiAT1G14650.1; AT1G14650.1; AT1G14650.
AT1G14650.2; AT1G14650.2; AT1G14650.
KEGGiath:AT1G14650.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC010657 Genomic DNA. Translation: AAF63169.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE29196.1.
CP002684 Genomic DNA. Translation: AEE29197.1.
AY081293 mRNA. Translation: AAL91182.1.
AK226440 mRNA. Translation: BAE98583.1.
PIRiG86280.
RefSeqiNP_001117289.1. NM_001123817.1.
NP_172917.1. NM_101332.4.
UniGeneiAt.22146.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WE6NMR-A683-780[»]
ProteinModelPortaliQ8RXF1.
SMRiQ8RXF1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8RXF1. 1 interactor.
STRINGi3702.AT1G14650.1.

PTM databases

iPTMnetiQ8RXF1.

Proteomic databases

PaxDbiQ8RXF1.
PRIDEiQ8RXF1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G14650.1; AT1G14650.1; AT1G14650.
AT1G14650.2; AT1G14650.2; AT1G14650.
GeneIDi838027.
GrameneiAT1G14650.1; AT1G14650.1; AT1G14650.
AT1G14650.2; AT1G14650.2; AT1G14650.
KEGGiath:AT1G14650.

Organism-specific databases

TAIRiAT1G14650.

Phylogenomic databases

eggNOGiKOG0007. Eukaryota.
ENOG410XPNW. LUCA.
HOGENOMiHOG000238941.
InParanoidiQ8RXF1.
KOiK12825.
OMAiGPARINV.
OrthoDBiEOG093605KI.
PhylomeDBiQ8RXF1.

Miscellaneous databases

EvolutionaryTraceiQ8RXF1.
PROiQ8RXF1.

Gene expression databases

GenevisibleiQ8RXF1. AT.

Family and domain databases

InterProiIPR022030. SF3A1.
IPR000061. Surp.
IPR029071. Ubiquitin-rel_dom.
IPR000626. Ubiquitin_dom.
[Graphical view]
PfamiPF12230. PRP21_like_P. 1 hit.
PF01805. Surp. 2 hits.
PF00240. ubiquitin. 1 hit.
[Graphical view]
SMARTiSM00648. SWAP. 2 hits.
SM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF109905. SSF109905. 2 hits.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50128. SURP. 2 hits.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSF3A1_ARATH
AccessioniPrimary (citable) accession number: Q8RXF1
Secondary accession number(s): Q0WWB5, Q9MA20
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: June 12, 2007
Last modified: November 30, 2016
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.