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Protein

Tryptophan--tRNA ligase, chloroplastic/mitochondrial

Gene

OVA4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei72ATPBy similarity1
Binding sitei197L-tryptophanBy similarity1
Binding sitei260ATP; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei272ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi78 – 81ATPBy similarity4
Nucleotide bindingi209 – 211ATPBy similarity3
Nucleotide bindingi269 – 273ATPBy similarity5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan--tRNA ligase, chloroplastic/mitochondrialCurated (EC:6.1.1.2Curated)
Alternative name(s):
Protein OVULE ABORTION 41 Publication
Tryptophanyl-tRNA synthetaseCurated
Short name:
TrpRSCurated
Gene namesi
Name:OVA41 Publication
Ordered Locus Names:At2g25840Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G25840

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Mitochondrion, Plastid

Pathology & Biotechi

Disruption phenotypei

Lethal. In heterozygous plants, aborted ovules.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 52Chloroplast and mitochondrionCombined sourcesAdd BLAST52
ChainiPRO_000043353953 – 408Tryptophan--tRNA ligase, chloroplastic/mitochondrialCuratedAdd BLAST356

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei53N-acetylserineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ8RXE9

PTM databases

iPTMnetiQ8RXE9

Expressioni

Gene expression databases

ExpressionAtlasiQ8RXE9 baseline and differential
GenevisibleiQ8RXE9 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G25840.2

Structurei

3D structure databases

ProteinModelPortaliQ8RXE9
SMRiQ8RXE9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi73 – 81"HIGH" regionCurated9
Motifi269 – 273"KMSKS" regionCurated5

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2713 Eukaryota
COG0180 LUCA
HOGENOMiHOG000059940
KOiK01867
PhylomeDBiQ8RXE9

Family and domain databases

CDDicd00806 TrpRS_core, 1 hit
Gene3Di3.40.50.6202 hits
HAMAPiMF_00140_B Trp_tRNA_synth_B, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002305 aa-tRNA-synth_Ic
IPR014729 Rossmann-like_a/b/a_fold
IPR002306 Trp-tRNA-ligase
IPR024109 Trp-tRNA-ligase_bac-type
PfamiView protein in Pfam
PF00579 tRNA-synt_1b, 1 hit
PRINTSiPR01039 TRNASYNTHTRP
TIGRFAMsiTIGR00233 trpS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.Curated
Isoform 1 (identifier: Q8RXE9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGHATSLSHF LILSSSRFSR LGSLTRLLSK PTSLSGSFSS ISVTGQGFRC
60 70 80 90 100
CCSVATDDTS PSVKKRVVSG VQPTGSVHLG NYLGAIKNWV ALQDTYETLF
110 120 130 140 150
IIVDHHAITL PYDTRQLGKA TTDTAALYLA CGIDVSKASV FVQSHVPAHV
160 170 180 190 200
ELMWLLCSST PIGWLQKMIQ FKEKSRKEGV ENASVGLFTY PDLMTADILL
210 220 230 240 250
YQSDFVPVGE DQKQHIELAR EIAQRVNHLY GGKKWKKLGG RGGSLFKIPE
260 270 280 290 300
PLIPQAGARV MSLTDGLSKM SKSAPSDQSR INLLDSKDLI VDKIKRCKTD
310 320 330 340 350
SFAGLEFDNA ERPECNNLLS IYQIVSGKKK EEVMEECKDM SWGTFKPLLA
360 370 380 390 400
DALIEHLSPI QARYQEIIAE PEYLDKILSE GADRAEELGA VTMRNMYQAM

GYYQRRRY
Length:408
Mass (Da):45,300
Last modified:June 1, 2002 - v1
Checksum:i48890DE7A3731636
GO

Sequence cautioni

The sequence AAC42246 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005395 Genomic DNA Translation: AAC42246.1 Sequence problems.
CP002685 Genomic DNA Translation: AEC07758.1
AY081299 mRNA Translation: AAL91188.1
BT001221 mRNA Translation: AAN65108.1
PIRiD84653
RefSeqiNP_850070.1, NM_179739.4 [Q8RXE9-1]
UniGeneiAt.13048

Genome annotation databases

EnsemblPlantsiAT2G25840.1; AT2G25840.1; AT2G25840 [Q8RXE9-1]
GeneIDi817126
GrameneiAT2G25840.1; AT2G25840.1; AT2G25840 [Q8RXE9-1]
KEGGiath:AT2G25840

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSYWM_ARATH
AccessioniPrimary (citable) accession number: Q8RXE9
Secondary accession number(s): O82313
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 22, 2015
Last sequence update: June 1, 2002
Last modified: April 25, 2018
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome