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Protein

Tryptophan--tRNA ligase, chloroplastic/mitochondrial

Gene

OVA4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei272ATPBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G25840-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan--tRNA ligase, chloroplastic/mitochondrialCurated (EC:6.1.1.2Curated)
Alternative name(s):
Protein OVULE ABORTION 41 Publication
Tryptophanyl-tRNA synthetaseCurated
Short name:
TrpRSCurated
Gene namesi
Name:OVA41 Publication
Ordered Locus Names:At2g25840Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G25840.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Mitochondrion, Plastid

Pathology & Biotechi

Disruption phenotypei

Lethal. In heterozygous plants, aborted ovules.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 52Chloroplast and mitochondrionCombined sourcesAdd BLAST52
ChainiPRO_000043353953 – 408Tryptophan--tRNA ligase, chloroplastic/mitochondrialCuratedAdd BLAST356

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei53N-acetylserineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ8RXE9.

Expressioni

Gene expression databases

ExpressionAtlasiQ8RXE9. baseline and differential.
GenevisibleiQ8RXE9. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G25840.2.

Structurei

3D structure databases

ProteinModelPortaliQ8RXE9.
SMRiQ8RXE9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi73 – 81"HIGH" regionCurated9
Motifi269 – 273"KMSKS" regionCurated5

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2713. Eukaryota.
COG0180. LUCA.
HOGENOMiHOG000059940.
KOiK01867.
PhylomeDBiQ8RXE9.

Family and domain databases

CDDicd00806. TrpRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00140_B. Trp_tRNA_synth_B. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR024109. Trp-tRNA-ligase_bac-type.
[Graphical view]
PANTHERiPTHR10055. PTHR10055. 1 hit.
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01039. TRNASYNTHTRP.
TIGRFAMsiTIGR00233. trpS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.Curated
Isoform 1 (identifier: Q8RXE9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGHATSLSHF LILSSSRFSR LGSLTRLLSK PTSLSGSFSS ISVTGQGFRC
60 70 80 90 100
CCSVATDDTS PSVKKRVVSG VQPTGSVHLG NYLGAIKNWV ALQDTYETLF
110 120 130 140 150
IIVDHHAITL PYDTRQLGKA TTDTAALYLA CGIDVSKASV FVQSHVPAHV
160 170 180 190 200
ELMWLLCSST PIGWLQKMIQ FKEKSRKEGV ENASVGLFTY PDLMTADILL
210 220 230 240 250
YQSDFVPVGE DQKQHIELAR EIAQRVNHLY GGKKWKKLGG RGGSLFKIPE
260 270 280 290 300
PLIPQAGARV MSLTDGLSKM SKSAPSDQSR INLLDSKDLI VDKIKRCKTD
310 320 330 340 350
SFAGLEFDNA ERPECNNLLS IYQIVSGKKK EEVMEECKDM SWGTFKPLLA
360 370 380 390 400
DALIEHLSPI QARYQEIIAE PEYLDKILSE GADRAEELGA VTMRNMYQAM

GYYQRRRY
Length:408
Mass (Da):45,300
Last modified:June 1, 2002 - v1
Checksum:i48890DE7A3731636
GO

Sequence cautioni

The sequence AAC42246 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005395 Genomic DNA. Translation: AAC42246.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC07758.1.
AY081299 mRNA. Translation: AAL91188.1.
BT001221 mRNA. Translation: AAN65108.1.
PIRiD84653.
RefSeqiNP_850070.1. NM_179739.4. [Q8RXE9-1]
UniGeneiAt.13048.

Genome annotation databases

EnsemblPlantsiAT2G25840.1; AT2G25840.1; AT2G25840. [Q8RXE9-1]
GeneIDi817126.
GrameneiAT2G25840.1; AT2G25840.1; AT2G25840.
KEGGiath:AT2G25840.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005395 Genomic DNA. Translation: AAC42246.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC07758.1.
AY081299 mRNA. Translation: AAL91188.1.
BT001221 mRNA. Translation: AAN65108.1.
PIRiD84653.
RefSeqiNP_850070.1. NM_179739.4. [Q8RXE9-1]
UniGeneiAt.13048.

3D structure databases

ProteinModelPortaliQ8RXE9.
SMRiQ8RXE9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G25840.2.

Proteomic databases

PaxDbiQ8RXE9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G25840.1; AT2G25840.1; AT2G25840. [Q8RXE9-1]
GeneIDi817126.
GrameneiAT2G25840.1; AT2G25840.1; AT2G25840.
KEGGiath:AT2G25840.

Organism-specific databases

TAIRiAT2G25840.

Phylogenomic databases

eggNOGiKOG2713. Eukaryota.
COG0180. LUCA.
HOGENOMiHOG000059940.
KOiK01867.
PhylomeDBiQ8RXE9.

Enzyme and pathway databases

BioCyciARA:AT2G25840-MONOMER.

Miscellaneous databases

PROiQ8RXE9.

Gene expression databases

ExpressionAtlasiQ8RXE9. baseline and differential.
GenevisibleiQ8RXE9. AT.

Family and domain databases

CDDicd00806. TrpRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00140_B. Trp_tRNA_synth_B. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR024109. Trp-tRNA-ligase_bac-type.
[Graphical view]
PANTHERiPTHR10055. PTHR10055. 1 hit.
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01039. TRNASYNTHTRP.
TIGRFAMsiTIGR00233. trpS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYWM_ARATH
AccessioniPrimary (citable) accession number: Q8RXE9
Secondary accession number(s): O82313
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 22, 2015
Last sequence update: June 1, 2002
Last modified: November 30, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.