E3 ubiquitin-protein ligase BRE1-like 1 - Arabidopsis thaliana (Mouse-ear cress)

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Q8RXD6 (BRE1A_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 85. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
E3 ubiquitin-protein ligase BRE1-like 1
Short name=AtBRE1
EC=6.3.2.-

Alternative name(s):
Protein HISTONE MONOUBIQUITINATION 1
Short name=AtHUB1

Gene names

Name:	HUB1
Synonyms:	BRE1A
Ordered Locus Names:	At2g44950/At2g44960
ORF Names:	T13E15.24/T13E15.23, T14P1.25/T14P1.24

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	878 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	E3 ubiquitin-protein ligase that monoubiquitinates H2B to form H2BK143ub1. H2BK143ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for H3K4me and maybe H3K79me. It thereby plays a central role in histone code and gene regulation. Forms a ubiquitin ligase complex in cooperation with the E2 enzyme UBC2/RAD6. Required for the regulation of flowering time and defense against necrotrophic fungal pathogens. Ref.5 Ref.6 Ref.7
Pathway	Protein modification; protein ubiquitination.
Subunit structure	May act as a tetramer consisting of two copies of HUB1 and two copies of HUB2 By similarity. Interacts with MED21. Ref.7
Subcellular location	Nucleus Ref.6 Ref.7.
Tissue specificity	Ubiquitously expressed. Ref.5 Ref.6
Developmental stage	Constant throughout the cell cycle. Ref.5
Induction	By infection with fungal pathogens. Ref.7
Domain	The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme By similarity.
Disruption phenotype	Plants have reduced seed dormancy and several pleiotropic phenotypes, including alterations in leaf color, plant architecture and flower morphology. Ref.6
Miscellaneous	HUB1 and HUB2 are involved in the same processes, but are weakly or not redundant.
Sequence similarities	Belongs to the BRE1 family. Contains 1 RING-type zinc finger.
Sequence caution	The sequence AAM14833.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes At2g44950 and At2g44960. The sequence AAM14834.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes At2g44950 and At2g44960.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Nucleus
Domain	Coiled coil Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Chromatin regulator Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell division Inferred from mutant phenotype Ref.5. Source: TAIR cell growth Inferred from mutant phenotype Ref.5. Source: TAIR defense response to fungus, incompatible interaction Inferred from mutant phenotype Ref.7. Source: TAIR histone H2B ubiquitination Inferred from mutant phenotype PubMed 18849490. Source: TAIR histone monoubiquitination Inferred from mutant phenotype Ref.6. Source: TAIR leaf morphogenesis Inferred from mutant phenotype Ref.5. Source: TAIR regulation of G2/M transition of mitotic cell cycle Inferred from mutant phenotype Ref.5. Source: TAIR seed dormancy process Inferred from mutant phenotype PubMed 12121467 Ref.6. Source: TAIR vegetative to reproductive phase transition of meristem Inferred from mutant phenotype PubMed 18849490. Source: TAIR
Cellular_component	mitochondrion Inferred from direct assay PubMed 14671022. Source: TAIR nucleus Inferred from direct assay Ref.7. Source: UniProtKB
Molecular_function	ubiquitin-protein ligase activity Inferred from direct assay Ref.5. Source: TAIR zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MED11	Q6ID77	2	EBI-2012188,EBI-1386244

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 878

878

E3 ubiquitin-protein ligase BRE1-like 1

PRO_0000293108

Regions

Zinc finger

826 – 865

RING-type

Coiled coil

48 – 76

Potential

Coiled coil

200 – 261

Potential

Coiled coil

293 – 382

Potential

Coiled coil

537 – 624

Potential

Compositional bias

22 – 25

Poly-Ala

Compositional bias

142 – 145

Poly-Ser

Experimental info

Mutagenesis

712 – 878

167

Missing in hub1-1/ang4-1; loss of function. Ref.5

Sequences

Sequence

Length

Mass (Da)

Tools

Q8RXD6 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 483445F94F87C291
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FASTA

878

99,719

        10         20         30         40         50         60 
MASTGEPDRK RRHFSSISPS EAAAAVKKQP FFWPSSEDKL DTAVLQFQNL KLSQKLEAQQ 

        70         80         90        100        110        120 
VECSILEDKL SQIKEKQLPY NSSLKTVHKS WEKLTASVES CSVRVSDSSS GAHRFVNKED 

       130        140        150        160        170        180 
GSSPAVKNDF INRLLETGAT ESSSSNICSN QMEENGVNTS SQMTQTLYNL VAATEDLRCL 

       190        200        210        220        230        240 
KDELYPTVLR TNLGKDLCGQ LALSELESEI KSFRGDLDDV LVKFKSLSRE LQSHRDADAK 

       250        260        270        280        290        300 
VRVDLKRIRG ELEDEVVELQ QCNGDLSALR AERDATAGAF FPVLSLGNKL ATSDRERDKQ 

       310        320        330        340        350        360 
RDLQDMETVL KELTVLASGR LQQLKNLHEE RTKMLGKMSN LQNKSKSVRC ISSSQACLSL 

       370        380        390        400        410        420 
KDQLEKSKEA VFQYMALLEK LQVEKDSIVW KEREINIKNE LGDVSRKTSA VTDSRMASLD 

       430        440        450        460        470        480 
SEIQKQLDEK MRIKTRLGNI SRERGRKEIF ADMKALISSF PEEMSSMRSQ LNNYKETAGG 

       490        500        510        520        530        540 
IHSLRADVQS LSGVLCRKTK EYEALQLRSA DYASQLGDLN ATVCDLKNSH EELKLFLDMY 

       550        560        570        580        590        600 
KRESTDARDI AEAKEQEYRA WAHVQSLKSS LDEQNLELRV KAANEAEAVS QQMLAAAEAE 

       610        620        630        640        650        660 
IADLRQKMDD CKRDVAKHSD ILKSKHEEHG TYLSEIQTIG SAYEDIVPQN QQLLLQVTER 

       670        680        690        700        710        720 
DDYNIKLFLE GITSRQMQDT LLIDKYIMDK DIQQGSAYAS FLSKKSSRIE DQLRFCTDQF 

       730        740        750        760        770        780 
QKLAEDKYQK SVSLENLQKK RADIGNGLEQ ARSRLEESHS KVEQSRLDYG ALELELEIER 

       790        800        810        820        830        840 
FNRRRIEEEM EIAKKKVSRL RSLIEGSSAI QKLRQELSEF KEILKCKACN DRPKEVVITK 

       850        860        870 
CYHLFCNPCV QKLTGTRQKK CPTCSASFGP NDIKPIYI

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"A conserved RING finger protein required for histone H2B monoubiquitination and cell size control." Hwang W.W., Venkatasubrahmanyam S., Ianculescu A.G., Tong A., Boone C., Madhani H.D. Mol. Cell 11:261-266(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
[5]	"The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth." Fleury D., Himanen K., Cnops G., Nelissen H., Boccardi T.M., Maere S., Beemster G.T.S., Neyt P., Anami S., Robles P., Micol J.L., Inze D., Van Lijsebettens M. Plant Cell 19:417-432(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF 712-GLN--ILE-878, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
[6]	"The absence of histone H2B monoubiquitination in the Arabidopsis hub1 (rdo4) mutant reveals a role for chromatin remodeling in seed dormancy." Liu Y., Koornneef M., Soppe W.J.J. Plant Cell 19:433-444(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
[7]	"HISTONE MONOUBIQUITINATION1 interacts with a subunit of the mediator complex and regulates defense against necrotrophic fungal pathogens in Arabidopsis." Dhawan R., Luo H., Foerster A.M., Abuqamar S., Du H.N., Briggs S.D., Mittelsten Scheid O., Mengiste T. Plant Cell 21:1000-1019(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH MED21, SUBCELLULAR LOCATION, INDUCTION.
+	Additional computationally mapped references.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC002388 Genomic DNA. Translation: AAM14833.1. Sequence problems. AC002388 Genomic DNA. Translation: AAM14834.1. Sequence problems. CP002685 Genomic DNA. Translation: AEC10489.1. BT010360 mRNA. Translation: AAQ56803.1. AY081322 mRNA. Translation: AAL91211.1.
IPI	IPI00518168.
PIR	T00397. T00398.
RefSeq	NP_182022.2. NM_130060.3.
UniGene	At.43266.
3D structure databases
HSSP	HSSP built from PDB template 1JM7 based on UniProtKB Q99728.
ProteinModelPortal	Q8RXD6.
SMR	Q8RXD6. Positions 817-877.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8RXD6. 37 interactions.
Proteomic databases
PaxDb	Q8RXD6.
PRIDE	Q8RXD6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G44950.1; AT2G44950.1; AT2G44950.
GeneID	819104.
KEGG	ath:AT2G44950.
Organism-specific databases
TAIR	At2g44950.
Phylogenomic databases
eggNOG	NOG263074.
HOGENOM	HOG000006004.
InParanoid	Q8RXD6.
KO	K10696.
OMA	FCNPCVQ.
PhylomeDB	Q8RXD6.
ProtClustDB	CLSN2680249.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
ArrayExpress	Q8RXD6.
Genevestigator	Q8RXD6.
Family and domain databases
Gene3D	3.30.40.10. 1 hit.
InterPro	IPR001841. Znf_RING. IPR013083. Znf_RING/FYVE/PHD. IPR017907. Znf_RING_CS. [Graphical view]
SMART	SM00184. RING. 1 hit. [Graphical view]
PROSITE	PS00518. ZF_RING_1. 1 hit. PS50089. ZF_RING_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BRE1A_ARATH

Accession

Primary (citable) accession number: Q8RXD6
Secondary accession number(s): O22156, O22157

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Web resources

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents