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Q8RXD6

- BRE1A_ARATH

UniProt

Q8RXD6 - BRE1A_ARATH

Protein

E3 ubiquitin-protein ligase BRE1-like 1

Gene

HUB1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    E3 ubiquitin-protein ligase that monoubiquitinates H2B to form H2BK143ub1. H2BK143ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for H3K4me and maybe H3K79me. It thereby plays a central role in histone code and gene regulation. Forms a ubiquitin ligase complex in cooperation with the E2 enzyme UBC2/RAD6. Required for the regulation of flowering time and defense against necrotrophic fungal pathogens.3 Publications

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri826 – 86540RING-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ligase activity Source: UniProtKB-KW
    2. protein binding Source: UniProtKB
    3. protein homodimerization activity Source: TAIR
    4. ubiquitin-protein transferase activity Source: TAIR
    5. zinc ion binding Source: InterPro

    GO - Biological processi

    1. cell division Source: TAIR
    2. cell growth Source: TAIR
    3. defense response to fungus, incompatible interaction Source: TAIR
    4. histone H2B ubiquitination Source: TAIR
    5. histone monoubiquitination Source: TAIR
    6. leaf morphogenesis Source: TAIR
    7. protein monoubiquitination Source: TAIR
    8. regulation of G2/M transition of mitotic cell cycle Source: TAIR
    9. seed dormancy process Source: TAIR
    10. vegetative to reproductive phase transition of meristem Source: TAIR

    Keywords - Molecular functioni

    Chromatin regulator, Ligase

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciARA:GQT-2085-MONOMER.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    E3 ubiquitin-protein ligase BRE1-like 1 (EC:6.3.2.-)
    Short name:
    AtBRE1
    Alternative name(s):
    Protein HISTONE MONOUBIQUITINATION 1
    Short name:
    AtHUB1
    Gene namesi
    Name:HUB1
    Synonyms:BRE1A
    Ordered Locus Names:At2g44950/At2g44960
    ORF Names:T13E15.24/T13E15.23, T14P1.25/T14P1.24
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G44950.

    Subcellular locationi

    Nucleus 2 Publications

    GO - Cellular componenti

    1. mitochondrion Source: TAIR
    2. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Plants have reduced seed dormancy and several pleiotropic phenotypes, including alterations in leaf color, plant architecture and flower morphology.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi712 – 878167Missing in hub1-1/ang4-1; loss of function. 1 PublicationAdd
    BLAST

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 878878E3 ubiquitin-protein ligase BRE1-like 1PRO_0000293108Add
    BLAST

    Proteomic databases

    PaxDbiQ8RXD6.
    PRIDEiQ8RXD6.

    Expressioni

    Tissue specificityi

    Ubiquitously expressed.2 Publications

    Developmental stagei

    Constant throughout the cell cycle.1 Publication

    Inductioni

    By infection with fungal pathogens.1 Publication

    Gene expression databases

    GenevestigatoriQ8RXD6.

    Interactioni

    Subunit structurei

    May act as a tetramer consisting of two copies of HUB1 and two copies of HUB2 By similarity. Interacts with MED21.By similarity1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    MED11Q6ID772EBI-2012188,EBI-1386244

    Protein-protein interaction databases

    BioGridi4440. 37 interactions.
    IntActiQ8RXD6. 37 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8RXD6.
    SMRiQ8RXD6. Positions 821-871.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili48 – 7629Sequence AnalysisAdd
    BLAST
    Coiled coili200 – 26162Sequence AnalysisAdd
    BLAST
    Coiled coili293 – 38290Sequence AnalysisAdd
    BLAST
    Coiled coili537 – 62488Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi22 – 254Poly-Ala
    Compositional biasi142 – 1454Poly-Ser

    Domaini

    The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.By similarity

    Sequence similaritiesi

    Belongs to the BRE1 family.Curated
    Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri826 – 86540RING-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Coiled coil, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG263074.
    HOGENOMiHOG000006004.
    InParanoidiQ8RXD6.
    KOiK10696.
    OMAiFCNPCVQ.
    PhylomeDBiQ8RXD6.

    Family and domain databases

    Gene3Di3.30.40.10. 1 hit.
    InterProiIPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR017907. Znf_RING_CS.
    [Graphical view]
    SMARTiSM00184. RING. 1 hit.
    [Graphical view]
    PROSITEiPS00518. ZF_RING_1. 1 hit.
    PS50089. ZF_RING_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8RXD6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASTGEPDRK RRHFSSISPS EAAAAVKKQP FFWPSSEDKL DTAVLQFQNL    50
    KLSQKLEAQQ VECSILEDKL SQIKEKQLPY NSSLKTVHKS WEKLTASVES 100
    CSVRVSDSSS GAHRFVNKED GSSPAVKNDF INRLLETGAT ESSSSNICSN 150
    QMEENGVNTS SQMTQTLYNL VAATEDLRCL KDELYPTVLR TNLGKDLCGQ 200
    LALSELESEI KSFRGDLDDV LVKFKSLSRE LQSHRDADAK VRVDLKRIRG 250
    ELEDEVVELQ QCNGDLSALR AERDATAGAF FPVLSLGNKL ATSDRERDKQ 300
    RDLQDMETVL KELTVLASGR LQQLKNLHEE RTKMLGKMSN LQNKSKSVRC 350
    ISSSQACLSL KDQLEKSKEA VFQYMALLEK LQVEKDSIVW KEREINIKNE 400
    LGDVSRKTSA VTDSRMASLD SEIQKQLDEK MRIKTRLGNI SRERGRKEIF 450
    ADMKALISSF PEEMSSMRSQ LNNYKETAGG IHSLRADVQS LSGVLCRKTK 500
    EYEALQLRSA DYASQLGDLN ATVCDLKNSH EELKLFLDMY KRESTDARDI 550
    AEAKEQEYRA WAHVQSLKSS LDEQNLELRV KAANEAEAVS QQMLAAAEAE 600
    IADLRQKMDD CKRDVAKHSD ILKSKHEEHG TYLSEIQTIG SAYEDIVPQN 650
    QQLLLQVTER DDYNIKLFLE GITSRQMQDT LLIDKYIMDK DIQQGSAYAS 700
    FLSKKSSRIE DQLRFCTDQF QKLAEDKYQK SVSLENLQKK RADIGNGLEQ 750
    ARSRLEESHS KVEQSRLDYG ALELELEIER FNRRRIEEEM EIAKKKVSRL 800
    RSLIEGSSAI QKLRQELSEF KEILKCKACN DRPKEVVITK CYHLFCNPCV 850
    QKLTGTRQKK CPTCSASFGP NDIKPIYI 878
    Length:878
    Mass (Da):99,719
    Last modified:June 1, 2002 - v1
    Checksum:i483445F94F87C291
    GO

    Sequence cautioni

    The sequence AAM14833.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes At2g44950 and At2g44960.
    The sequence AAM14834.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes At2g44950 and At2g44960.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC002388 Genomic DNA. Translation: AAM14833.1. Sequence problems.
    AC002388 Genomic DNA. Translation: AAM14834.1. Sequence problems.
    CP002685 Genomic DNA. Translation: AEC10489.1.
    BT010360 mRNA. Translation: AAQ56803.1.
    AY081322 mRNA. Translation: AAL91211.1.
    PIRiT00397.
    T00398.
    RefSeqiNP_182022.2. NM_130060.3.
    UniGeneiAt.43266.

    Genome annotation databases

    EnsemblPlantsiAT2G44950.1; AT2G44950.1; AT2G44950.
    GeneIDi819104.
    KEGGiath:AT2G44950.

    Cross-referencesi

    Web resourcesi

    PlantsUBQ

    A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC002388 Genomic DNA. Translation: AAM14833.1 . Sequence problems.
    AC002388 Genomic DNA. Translation: AAM14834.1 . Sequence problems.
    CP002685 Genomic DNA. Translation: AEC10489.1 .
    BT010360 mRNA. Translation: AAQ56803.1 .
    AY081322 mRNA. Translation: AAL91211.1 .
    PIRi T00397.
    T00398.
    RefSeqi NP_182022.2. NM_130060.3.
    UniGenei At.43266.

    3D structure databases

    ProteinModelPortali Q8RXD6.
    SMRi Q8RXD6. Positions 821-871.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 4440. 37 interactions.
    IntActi Q8RXD6. 37 interactions.

    Proteomic databases

    PaxDbi Q8RXD6.
    PRIDEi Q8RXD6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G44950.1 ; AT2G44950.1 ; AT2G44950 .
    GeneIDi 819104.
    KEGGi ath:AT2G44950.

    Organism-specific databases

    TAIRi AT2G44950.

    Phylogenomic databases

    eggNOGi NOG263074.
    HOGENOMi HOG000006004.
    InParanoidi Q8RXD6.
    KOi K10696.
    OMAi FCNPCVQ.
    PhylomeDBi Q8RXD6.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    BioCyci ARA:GQT-2085-MONOMER.

    Miscellaneous databases

    PROi Q8RXD6.

    Gene expression databases

    Genevestigatori Q8RXD6.

    Family and domain databases

    Gene3Di 3.30.40.10. 1 hit.
    InterProi IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR017907. Znf_RING_CS.
    [Graphical view ]
    SMARTi SM00184. RING. 1 hit.
    [Graphical view ]
    PROSITEi PS00518. ZF_RING_1. 1 hit.
    PS50089. ZF_RING_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "A conserved RING finger protein required for histone H2B monoubiquitination and cell size control."
      Hwang W.W., Venkatasubrahmanyam S., Ianculescu A.G., Tong A., Boone C., Madhani H.D.
      Mol. Cell 11:261-266(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION.
    5. "The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth."
      Fleury D., Himanen K., Cnops G., Nelissen H., Boccardi T.M., Maere S., Beemster G.T.S., Neyt P., Anami S., Robles P., Micol J.L., Inze D., Van Lijsebettens M.
      Plant Cell 19:417-432(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF 712-GLN--ILE-878, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
    6. "The absence of histone H2B monoubiquitination in the Arabidopsis hub1 (rdo4) mutant reveals a role for chromatin remodeling in seed dormancy."
      Liu Y., Koornneef M., Soppe W.J.J.
      Plant Cell 19:433-444(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
    7. "HISTONE MONOUBIQUITINATION1 interacts with a subunit of the mediator complex and regulates defense against necrotrophic fungal pathogens in Arabidopsis."
      Dhawan R., Luo H., Foerster A.M., Abuqamar S., Du H.N., Briggs S.D., Mittelsten Scheid O., Mengiste T.
      Plant Cell 21:1000-1019(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH MED21, SUBCELLULAR LOCATION, INDUCTION.

    Entry informationi

    Entry nameiBRE1A_ARATH
    AccessioniPrimary (citable) accession number: Q8RXD6
    Secondary accession number(s): O22156, O22157
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 10, 2007
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 98 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    HUB1 and HUB2 are involved in the same processes, but are weakly or not redundant.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3