SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q8RXD6

- BRE1A_ARATH

UniProt

Q8RXD6 - BRE1A_ARATH

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
E3 ubiquitin-protein ligase BRE1-like 1
Gene
HUB1, BRE1A, At2g44950/At2g44960, T13E15.24/T13E15.23, T14P1.25/T14P1.24
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that monoubiquitinates H2B to form H2BK143ub1. H2BK143ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for H3K4me and maybe H3K79me. It thereby plays a central role in histone code and gene regulation. Forms a ubiquitin ligase complex in cooperation with the E2 enzyme UBC2/RAD6. Required for the regulation of flowering time and defense against necrotrophic fungal pathogens.3 Publications

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri826 – 86540RING-type
Add
BLAST

GO - Molecular functioni

  1. ligase activity Source: UniProtKB-KW
  2. protein binding Source: UniProtKB
  3. protein homodimerization activity Source: TAIR
  4. ubiquitin-protein transferase activity Source: TAIR
  5. zinc ion binding Source: InterPro

GO - Biological processi

  1. cell division Source: TAIR
  2. cell growth Source: TAIR
  3. defense response to fungus, incompatible interaction Source: TAIR
  4. histone H2B ubiquitination Source: TAIR
  5. histone monoubiquitination Source: TAIR
  6. leaf morphogenesis Source: TAIR
  7. protein monoubiquitination Source: TAIR
  8. regulation of G2/M transition of mitotic cell cycle Source: TAIR
  9. seed dormancy process Source: TAIR
  10. vegetative to reproductive phase transition of meristem Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciARA:GQT-2085-MONOMER.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase BRE1-like 1 (EC:6.3.2.-)
Short name:
AtBRE1
Alternative name(s):
Protein HISTONE MONOUBIQUITINATION 1
Short name:
AtHUB1
Gene namesi
Name:HUB1
Synonyms:BRE1A
Ordered Locus Names:At2g44950/At2g44960
ORF Names:T13E15.24/T13E15.23, T14P1.25/T14P1.24
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G44950.

Subcellular locationi

Nucleus 2 Publications

GO - Cellular componenti

  1. mitochondrion Source: TAIR
  2. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Plants have reduced seed dormancy and several pleiotropic phenotypes, including alterations in leaf color, plant architecture and flower morphology.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi712 – 878167Missing in hub1-1/ang4-1; loss of function. 1 Publication
Add
BLAST

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 878878E3 ubiquitin-protein ligase BRE1-like 1
PRO_0000293108Add
BLAST

Proteomic databases

PaxDbiQ8RXD6.
PRIDEiQ8RXD6.

Expressioni

Tissue specificityi

Ubiquitously expressed.2 Publications

Developmental stagei

Constant throughout the cell cycle.1 Publication

Inductioni

By infection with fungal pathogens.1 Publication

Gene expression databases

GenevestigatoriQ8RXD6.

Interactioni

Subunit structurei

May act as a tetramer consisting of two copies of HUB1 and two copies of HUB2 By similarity. Interacts with MED21.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
MED11Q6ID772EBI-2012188,EBI-1386244

Protein-protein interaction databases

BioGridi4440. 37 interactions.
IntActiQ8RXD6. 37 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ8RXD6.
SMRiQ8RXD6. Positions 821-871.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili48 – 7629 Reviewed prediction
Add
BLAST
Coiled coili200 – 26162 Reviewed prediction
Add
BLAST
Coiled coili293 – 38290 Reviewed prediction
Add
BLAST
Coiled coili537 – 62488 Reviewed prediction
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi22 – 254Poly-Ala
Compositional biasi142 – 1454Poly-Ser

Domaini

The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme By similarity.

Sequence similaritiesi

Belongs to the BRE1 family.

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiNOG263074.
HOGENOMiHOG000006004.
InParanoidiQ8RXD6.
KOiK10696.
OMAiFCNPCVQ.
PhylomeDBiQ8RXD6.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8RXD6-1 [UniParc]FASTAAdd to Basket

« Hide

MASTGEPDRK RRHFSSISPS EAAAAVKKQP FFWPSSEDKL DTAVLQFQNL    50
KLSQKLEAQQ VECSILEDKL SQIKEKQLPY NSSLKTVHKS WEKLTASVES 100
CSVRVSDSSS GAHRFVNKED GSSPAVKNDF INRLLETGAT ESSSSNICSN 150
QMEENGVNTS SQMTQTLYNL VAATEDLRCL KDELYPTVLR TNLGKDLCGQ 200
LALSELESEI KSFRGDLDDV LVKFKSLSRE LQSHRDADAK VRVDLKRIRG 250
ELEDEVVELQ QCNGDLSALR AERDATAGAF FPVLSLGNKL ATSDRERDKQ 300
RDLQDMETVL KELTVLASGR LQQLKNLHEE RTKMLGKMSN LQNKSKSVRC 350
ISSSQACLSL KDQLEKSKEA VFQYMALLEK LQVEKDSIVW KEREINIKNE 400
LGDVSRKTSA VTDSRMASLD SEIQKQLDEK MRIKTRLGNI SRERGRKEIF 450
ADMKALISSF PEEMSSMRSQ LNNYKETAGG IHSLRADVQS LSGVLCRKTK 500
EYEALQLRSA DYASQLGDLN ATVCDLKNSH EELKLFLDMY KRESTDARDI 550
AEAKEQEYRA WAHVQSLKSS LDEQNLELRV KAANEAEAVS QQMLAAAEAE 600
IADLRQKMDD CKRDVAKHSD ILKSKHEEHG TYLSEIQTIG SAYEDIVPQN 650
QQLLLQVTER DDYNIKLFLE GITSRQMQDT LLIDKYIMDK DIQQGSAYAS 700
FLSKKSSRIE DQLRFCTDQF QKLAEDKYQK SVSLENLQKK RADIGNGLEQ 750
ARSRLEESHS KVEQSRLDYG ALELELEIER FNRRRIEEEM EIAKKKVSRL 800
RSLIEGSSAI QKLRQELSEF KEILKCKACN DRPKEVVITK CYHLFCNPCV 850
QKLTGTRQKK CPTCSASFGP NDIKPIYI 878
Length:878
Mass (Da):99,719
Last modified:June 1, 2002 - v1
Checksum:i483445F94F87C291
GO

Sequence cautioni

The sequence AAM14833.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes At2g44950 and At2g44960.
The sequence AAM14834.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes At2g44950 and At2g44960.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC002388 Genomic DNA. Translation: AAM14833.1. Sequence problems.
AC002388 Genomic DNA. Translation: AAM14834.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC10489.1.
BT010360 mRNA. Translation: AAQ56803.1.
AY081322 mRNA. Translation: AAL91211.1.
PIRiT00397.
T00398.
RefSeqiNP_182022.2. NM_130060.3.
UniGeneiAt.43266.

Genome annotation databases

EnsemblPlantsiAT2G44950.1; AT2G44950.1; AT2G44950.
GeneIDi819104.
KEGGiath:AT2G44950.

Cross-referencesi

Web resourcesi

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC002388 Genomic DNA. Translation: AAM14833.1 . Sequence problems.
AC002388 Genomic DNA. Translation: AAM14834.1 . Sequence problems.
CP002685 Genomic DNA. Translation: AEC10489.1 .
BT010360 mRNA. Translation: AAQ56803.1 .
AY081322 mRNA. Translation: AAL91211.1 .
PIRi T00397.
T00398.
RefSeqi NP_182022.2. NM_130060.3.
UniGenei At.43266.

3D structure databases

ProteinModelPortali Q8RXD6.
SMRi Q8RXD6. Positions 821-871.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 4440. 37 interactions.
IntActi Q8RXD6. 37 interactions.

Proteomic databases

PaxDbi Q8RXD6.
PRIDEi Q8RXD6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G44950.1 ; AT2G44950.1 ; AT2G44950 .
GeneIDi 819104.
KEGGi ath:AT2G44950.

Organism-specific databases

TAIRi AT2G44950.

Phylogenomic databases

eggNOGi NOG263074.
HOGENOMi HOG000006004.
InParanoidi Q8RXD6.
KOi K10696.
OMAi FCNPCVQ.
PhylomeDBi Q8RXD6.

Enzyme and pathway databases

UniPathwayi UPA00143 .
BioCyci ARA:GQT-2085-MONOMER.

Miscellaneous databases

PROi Q8RXD6.

Gene expression databases

Genevestigatori Q8RXD6.

Family and domain databases

Gene3Di 3.30.40.10. 1 hit.
InterProi IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view ]
SMARTi SM00184. RING. 1 hit.
[Graphical view ]
PROSITEi PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "A conserved RING finger protein required for histone H2B monoubiquitination and cell size control."
    Hwang W.W., Venkatasubrahmanyam S., Ianculescu A.G., Tong A., Boone C., Madhani H.D.
    Mol. Cell 11:261-266(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  5. "The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth."
    Fleury D., Himanen K., Cnops G., Nelissen H., Boccardi T.M., Maere S., Beemster G.T.S., Neyt P., Anami S., Robles P., Micol J.L., Inze D., Van Lijsebettens M.
    Plant Cell 19:417-432(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF 712-GLN--ILE-878, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
  6. "The absence of histone H2B monoubiquitination in the Arabidopsis hub1 (rdo4) mutant reveals a role for chromatin remodeling in seed dormancy."
    Liu Y., Koornneef M., Soppe W.J.J.
    Plant Cell 19:433-444(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
  7. "HISTONE MONOUBIQUITINATION1 interacts with a subunit of the mediator complex and regulates defense against necrotrophic fungal pathogens in Arabidopsis."
    Dhawan R., Luo H., Foerster A.M., Abuqamar S., Du H.N., Briggs S.D., Mittelsten Scheid O., Mengiste T.
    Plant Cell 21:1000-1019(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MED21, SUBCELLULAR LOCATION, INDUCTION.

Entry informationi

Entry nameiBRE1A_ARATH
AccessioniPrimary (citable) accession number: Q8RXD6
Secondary accession number(s): O22156, O22157
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: June 1, 2002
Last modified: September 3, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

HUB1 and HUB2 are involved in the same processes, but are weakly or not redundant.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi