Q8RXA8 (NDK4_SPIOL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleoside diphosphate kinase 4, chloroplastic
EC=2.7.4.6

Alternative name(s):
Nucleoside diphosphate kinase III
Nucleoside diphosphate kinase IV
Short name=NDK IV
Short name=NDP kinase IV
Short name=NDPK IV

Gene names

Name:	NDK4
Synonyms:	NDPKIII

Organism

Spinacia oleracea (Spinach)

Taxonomic identifier

3562 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Amaranthaceae › Spinacia

Protein attributes

Sequence length	235 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Shows the highest specificity towards GDP By similarity.
Catalytic activity	ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.
Cofactor	Magnesium By similarity.
Subunit structure	Homohexamer Probable.
Subcellular location	Plastid › chloroplast thylakoid lumen Ref.2.
Sequence similarities	Belongs to the NDK family.
Caution	There are likely two genes encoding for two slightly different proteins, NDK3 and NDK4. The characterization was made on a thylakoid lumen preparation containing probably both proteins.
Biophysicochemical properties	Kinetic parameters: KM=27.4 µM for GDP KM=89.05 µM for ADP pH dependence: Optimum pH is 6.0.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Cellular component	Chloroplast Plastid Thylakoid
Domain	Transit peptide
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	CTP biosynthetic process Inferred from electronic annotation. Source: InterPro GTP biosynthetic process Inferred from electronic annotation. Source: InterPro UTP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	chloroplast thylakoid lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside diphosphate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Chloroplast Probable
	Transit peptide	? – 82		Thylakoid
	Chain	83 – 235	153	Nucleoside diphosphate kinase 4, chloroplastic		PRO_0000019441
Sites
	Active site	199	1	Pros-phosphohistidine intermediate By similarity
	Binding site	93	1	ATP By similarity
	Binding site	141	1	ATP By similarity
	Binding site	169	1	ATP By similarity
	Binding site	175	1	ATP By similarity
	Binding site	186	1	ATP By similarity
	Binding site	196	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8RXA8 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: AB60AC38AAB977D5	FASTA	235	25,685
	10 20 30 40 50 60 MRSQIYRSAT KAARSFLSSS KNASSRFLPE GRTVAATAAV SLRVKAPYLA SFGGANASGT 70 80 90 100 110 120 WMSTALAIPA AAYLLQDQEA CAAEFERTFI AIKPDGVQRG LISEIVARFE RKGFKLVAIK 130 140 150 160 170 180 VVIPSKDFAQ KHYHDLSERP FFNGLCDFLS SGPVVAMVWE GEGVIKYGRK LIGATDPQKS 190 200 210 220 230 EPGTIRGDLA VVVGRNIIHG SDGPETAKDE IKLWFKPEEL VNYTHNAEKW IYGDN

References

[1]	"Spinach mRNA for nucleoside diphosphate kinase III." Zhang J., Hu Y. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Multiple evidence for nucleotide metabolism in the chloroplast thylakoid lumen." Spetea C., Hundal T., Lundin B., Heddad M., Adamska I., Andersson B. Proc. Natl. Acad. Sci. U.S.A. 101:1409-1414(2004) [PubMed: 14736920] [Abstract] Cited for: SUBCELLULAR LOCATION, CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY082428 mRNA. Translation: AAL91136.1.
3D structure databases
ProteinModelPortal	Q8RXA8.
SMR	Q8RXA8. Positions 83-233.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001564. Nucleoside_diP_kinase. IPR023005. Nucleoside_diP_kinase_AS. [Graphical view]
Gene3D	G3DSA:3.30.70.141. NDK. 1 hit.
PANTHER	PTHR11349. Nuc_diP_kinase_core. 1 hit.
Pfam	PF00334. NDK. 1 hit. [Graphical view]
PRINTS	PR01243. NUCDPKINASE.
SMART	SM00562. NDK. 1 hit. [Graphical view]
SUPFAM	SSF54919. NDK. 1 hit.
PROSITE	PS00469. NDP_KINASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDK4_SPIOL

Accession

Primary (citable) accession number: Q8RXA8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2004
Last sequence update:	June 1, 2002
Last modified:	May 31, 2011
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program