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Protein

CAAX prenyl protease 1 homolog

Gene

FACE1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Proteolytically removes the C-terminal three residues of farnesylated proteins. The substrate specificity is only partially overlapping with that of FACE2.1 Publication

Catalytic activityi

The peptide bond hydrolyzed can be designated -C-|-A-A-X in which C is an S-isoprenylated cysteine residue, A is usually aliphatic and X is the C-terminal residue of the substrate protein, and may be any of several amino acids.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi284Zinc; catalyticBy similarity1
Active sitei285By similarity1
Metal bindingi288Zinc; catalyticBy similarity1
Metal bindingi362Zinc; catalyticBy similarity1
Active sitei366Proton donorBy similarity1

GO - Molecular functioni

  • endopeptidase activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW
  • metalloendopeptidase activity Source: TAIR

GO - Biological processi

  • CAAX-box protein maturation Source: TAIR
  • CAAX-box protein processing Source: GO_Central
  • proteolysis Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.4.24.84. 399.

Protein family/group databases

MEROPSiM48.003.
TCDBi9.B.1.1.2. the integral membrane caax protease (caax protease) family.

Names & Taxonomyi

Protein namesi
Recommended name:
CAAX prenyl protease 1 homolog (EC:3.4.24.84)
Alternative name(s):
Farnesylated proteins-converting enzyme 1
Short name:
AtFACE-1
Short name:
FACE-1
Prenyl protein-specific endoprotease 1
Zinc metalloproteinase Ste24 homolog
Short name:
AtSTE24
Gene namesi
Name:FACE1
Synonyms:STE24
Ordered Locus Names:At4g01320
ORF Names:A_IG002N01.21, F2N1.21
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G01320.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei3 – 23HelicalSequence analysisAdd BLAST21
Transmembranei67 – 87HelicalSequence analysisAdd BLAST21
Transmembranei109 – 129HelicalSequence analysisAdd BLAST21
Transmembranei155 – 175HelicalSequence analysisAdd BLAST21
Transmembranei185 – 205HelicalSequence analysisAdd BLAST21
Transmembranei295 – 315HelicalSequence analysisAdd BLAST21
Transmembranei332 – 352HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • endoplasmic reticulum Source: TAIR
  • integral component of endoplasmic reticulum membrane Source: GO_Central
  • vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi284H → A: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003562401 – 424CAAX prenyl protease 1 homologAdd BLAST424

Proteomic databases

PaxDbiQ8RX88.
PRIDEiQ8RX88.

Expressioni

Tissue specificityi

Expressed in leaves, stems and flowers.1 Publication

Gene expression databases

GenevisibleiQ8RX88. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G01320.1.

Structurei

3D structure databases

ProteinModelPortaliQ8RX88.
SMRiQ8RX88.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M48A family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2719. Eukaryota.
COG0501. LUCA.
HOGENOMiHOG000257874.
InParanoidiQ8RX88.
KOiK06013.
OMAiKKQGCNN.
OrthoDBiEOG09360AJP.
PhylomeDBiQ8RX88.

Family and domain databases

InterProiIPR027057. CAXX_Prtase_1.
IPR001915. Peptidase_M48.
IPR032456. Peptidase_M48_N.
[Graphical view]
PANTHERiPTHR10120:SF24. PTHR10120:SF24. 1 hit.
PfamiPF01435. Peptidase_M48. 1 hit.
PF16491. Peptidase_M48_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8RX88-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIPFMETVV GFMIVMYIFE TYLDLRQLTA LKLPTLPKTL VGVISQEKFE
60 70 80 90 100
KSRAYSLDKS YFHFVHEFVT ILMDSAILFF GILPWFWKMS GAVLPRLGLD
110 120 130 140 150
PENEILHTLS FLAGVMTWSQ ITDLPFSLYS TFVIESRHGF NKQTIWMFIR
160 170 180 190 200
DMIKGTFLSV ILGPPIVAAI IFIVQKGGPY LAIYLWAFMF ILSLVMMTIY
210 220 230 240 250
PVLIAPLFNK FTPLPDGDLR EKIEKLASSL KFPLKKLFVV DGSTRSSHSN
260 270 280 290 300
AYMYGFFKNK RIVLYDTLIQ QCKNEDEIVA VIAHELGHWK LNHTTYSFIA
310 320 330 340 350
VQILAFLQFG GYTLVRNSTD LFRSFGFDTQ PVLIGLIIFQ HTVIPLQHLV
360 370 380 390 400
SFGLNLVSRA FEFQADAFAV KLGYAKDLRP ALVKLQEENL SAMNTDPLYS
410 420
AYHYSHPPLV ERLRAIDGED KKTD
Length:424
Mass (Da):48,482
Last modified:June 1, 2002 - v1
Checksum:iB894AE17C8044857
GO

Sequence cautioni

The sequence AAB61028 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB80941 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti81G → W in AAL07084 (PubMed:14593172).Curated1
Sequence conflicti266D → G in AAL07084 (PubMed:14593172).Curated1
Sequence conflicti399Y → H in AAK39514 (PubMed:12039957).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF353722 mRNA. Translation: AAK39514.1.
AF007269 Genomic DNA. Translation: AAB61028.1. Sequence problems.
AL161491 Genomic DNA. Translation: CAB80941.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE82008.1.
AY056235 mRNA. Translation: AAL07084.1.
AY090232 mRNA. Translation: AAL90896.1.
BT006355 mRNA. Translation: AAP21163.1.
PIRiC85017.
T01712.
RefSeqiNP_567212.1. NM_116362.3.
UniGeneiAt.3809.

Genome annotation databases

EnsemblPlantsiAT4G01320.1; AT4G01320.1; AT4G01320.
GeneIDi828209.
GrameneiAT4G01320.1; AT4G01320.1; AT4G01320.
KEGGiath:AT4G01320.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF353722 mRNA. Translation: AAK39514.1.
AF007269 Genomic DNA. Translation: AAB61028.1. Sequence problems.
AL161491 Genomic DNA. Translation: CAB80941.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE82008.1.
AY056235 mRNA. Translation: AAL07084.1.
AY090232 mRNA. Translation: AAL90896.1.
BT006355 mRNA. Translation: AAP21163.1.
PIRiC85017.
T01712.
RefSeqiNP_567212.1. NM_116362.3.
UniGeneiAt.3809.

3D structure databases

ProteinModelPortaliQ8RX88.
SMRiQ8RX88.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT4G01320.1.

Protein family/group databases

MEROPSiM48.003.
TCDBi9.B.1.1.2. the integral membrane caax protease (caax protease) family.

Proteomic databases

PaxDbiQ8RX88.
PRIDEiQ8RX88.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G01320.1; AT4G01320.1; AT4G01320.
GeneIDi828209.
GrameneiAT4G01320.1; AT4G01320.1; AT4G01320.
KEGGiath:AT4G01320.

Organism-specific databases

TAIRiAT4G01320.

Phylogenomic databases

eggNOGiKOG2719. Eukaryota.
COG0501. LUCA.
HOGENOMiHOG000257874.
InParanoidiQ8RX88.
KOiK06013.
OMAiKKQGCNN.
OrthoDBiEOG09360AJP.
PhylomeDBiQ8RX88.

Enzyme and pathway databases

BRENDAi3.4.24.84. 399.

Miscellaneous databases

PROiQ8RX88.

Gene expression databases

GenevisibleiQ8RX88. AT.

Family and domain databases

InterProiIPR027057. CAXX_Prtase_1.
IPR001915. Peptidase_M48.
IPR032456. Peptidase_M48_N.
[Graphical view]
PANTHERiPTHR10120:SF24. PTHR10120:SF24. 1 hit.
PfamiPF01435. Peptidase_M48. 1 hit.
PF16491. Peptidase_M48_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFACE1_ARATH
AccessioniPrimary (citable) accession number: Q8RX88
Secondary accession number(s): O04602
, Q93ZV9, Q94FS8, Q9M139
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 16, 2008
Last sequence update: June 1, 2002
Last modified: November 30, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.