Q8RX87 (RFS6_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable galactinol--sucrose galactosyltransferase 6
EC=2.4.1.82

Alternative name(s):
Protein DARK INDUCIBLE 10
Raffinose synthase 6

Gene names

Name:	RFS6
Synonyms:	DIN10, RS6
Ordered Locus Names:	At5g20250
ORF Names:	F5O24.140

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	749 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Transglycosidase operating by a ping-pong reaction mechanism. Involved in the synthesis of raffinose, a major soluble carbohydrate in seeds, roots and tubers By similarity.
Catalytic activity	Alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose = myo-inositol + raffinose.
Developmental stage	Expressed in 24 hours imbibed seeds and during leaf senescence. Ref.6
Induction	By oxidative stress and by dark treatment. Down-regulated by sucrose, but not by osmotic treatment. Ref.6 Ref.7
Sequence similarities	Belongs to the glycosyl hydrolases 36 family.
Sequence caution	The sequence AAL90901.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence AAN18198.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence BAE99252.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Coding sequence diversity	Alternative splicing
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	galactinol-sucrose galactosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q8RX87-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 749	749	Probable galactinol--sucrose galactosyltransferase 6		PRO_0000389259
Experimental info
	Sequence conflict	302	1	K → R in BAH19983. Ref.5
	Sequence conflict	670	1	M → V in BAD93984. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 24, 2009. Version 2. Checksum: B8A9DEBB6A2407E7	FASTA	749	83,116
	10 20 30 40 50 60 MTIKPAVRIS DGNLIIKNRT ILTGVPDNVI TTSASEAGPV EGVFVGAVFN KEESKHIVPI 70 80 90 100 110 120 GTLRNSRFMS CFRFKLWWMA QRMGEMGRDI PYETQFLLVE SNDGSHLESD GANGVECNQK 130 140 150 160 170 180 VYTVFLPLIE GSFRSCLQGN VNDEVELCLE SGDVDTKRSS FTHSLYIHAG TDPFQTITDA 190 200 210 220 230 240 IRTVKLHLNS FRQRHEKKLP GIVDYFGWCT WDAFYQEVTQ EGVEAGLKSL AAGGTPPKFV 250 260 270 280 290 300 IIDDGWQSVE RDATVEAGDE KKESPIFRLT GIKENEKFKK KDDPNVGIKN IVKIAKEKHG 310 320 330 340 350 360 LKYVYVWHAI TGYWGGVRPG EEYGSVMKYP NMSKGVVEND PTWKTDVMTL QGLGLVSPKK 370 380 390 400 410 420 VYKFYNELHS YLADAGVDGV KVDVQCVLET LGGGLGGRVE LTRQFHQALD SSVAKNFPDN 430 440 450 460 470 480 GCIACMSHNT DALYCSKQAA VIRASDDFYP RDPVSHTIHI ASVAYNSVFL GEFMQPDWDM 490 500 510 520 530 540 FHSVHPAAEY HASARAISGG PLYVSDSPGK HNFELLRKLV LPDGSILRAR LPGRPTRDCL 550 560 570 580 590 600 FADPARDGVS LLKIWNMNKY TGVLGVYNCQ GAAWSSTERK NIFHQTKTDS LTGSIRGRDV 610 620 630 640 650 660 HSISEASTDP TTWNGDCAVY SQSRGELIVM PYNVSLPVSL KIREHEIFTV SPISHLVDGV 670 680 690 700 710 720 SFAPIGLVNM YNSGGAIEGL RYEAEKMKVV MEVKGCGKFG SYSSVKPKRC VVESNEIAFE 730 740 YDSSSGLVTF ELDKMPIENK RFHLIQVEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana." Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F. Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs." Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K. DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Dark-inducible genes from Arabidopsis thaliana are associated with leaf senescence and repressed by sugars." Fujiki Y., Yoshikawa Y., Sato T., Inada N., Ito M., Nishida I., Watanabe A. Physiol. Plantarum 111:345-352(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION BY DARK AND SUCROSE, DEVELOPMENTAL STAGE.
[7]	"Galactinol and raffinose constitute a novel function to protect plants from oxidative damage." Nishizawa A., Yabuta Y., Shigeoka S. Plant Physiol. 147:1251-1263(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION BY OXIDATIVE STRESS, GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF296825 Genomic DNA. No translation available. CP002688 Genomic DNA. Translation: AED92818.1. CP002688 Genomic DNA. Translation: AED92819.1. CP002688 Genomic DNA. Translation: AED92820.1. AY090237 mRNA. Translation: AAL90901.1. Different initiation. BT000632 mRNA. Translation: AAN18198.1. Different initiation. AK227214 mRNA. Translation: BAE99252.1. Different initiation. AK221808 mRNA. Translation: BAD93984.1. AK316714 mRNA. Translation: BAH19441.1. AK317307 mRNA. Translation: BAH19983.1. AK318923 mRNA. Translation: BAH57038.1.
IPI	IPI00523317.
RefSeq	NP_001031910.1. NM_001036833.2. NP_001190347.1. NM_001203418.1. NP_197525.1. NM_122032.5. NP_851044.2. NM_180713.3.
UniGene	At.25459. At.74986.
3D structure databases
ProteinModelPortal	Q8RX87.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8RX87. 2 interactions.
Protein family/group databases
CAZy	GH36. Glycoside Hydrolase Family 36.
Proteomic databases
PaxDb	Q8RX87.
PRIDE	Q8RX87.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G20250.1; AT5G20250.1; AT5G20250. AT5G20250.2; AT5G20250.2; AT5G20250. AT5G20250.3; AT5G20250.3; AT5G20250.
GeneID	832147.
KEGG	ath:AT5G20250.
Organism-specific databases
TAIR	At5g20250.
Phylogenomic databases
eggNOG	NOG06986.
HOGENOM	HOG000237551.
InParanoid	Q8RX87.
KO	K06617.
PhylomeDB	Q8RX87.
ProtClustDB	PLN02684.
Gene expression databases
ArrayExpress	Q8RX87.
Family and domain databases
Gene3D	3.20.20.70. 2 hits.
InterPro	IPR013785. Aldolase_TIM. IPR017853. Glycoside_hydrolase_SF. IPR008811. Raffinose_synthase. [Graphical view]
Pfam	PF05691. Raffinose_syn. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RFS6_ARATH

Accession

Primary (citable) accession number: Q8RX87
Secondary accession number(s): B9DFC4, B9DGW6, Q56X69

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 24, 2009
Last sequence update:	November 24, 2009
Last modified:	May 1, 2013
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program