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Q8RX87 (RFS6_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable galactinol--sucrose galactosyltransferase 6

EC=2.4.1.82
Alternative name(s):
Protein DARK INDUCIBLE 10
Raffinose synthase 6
Gene names
Name:RFS6
Synonyms:DIN10, RS6
Ordered Locus Names:At5g20250
ORF Names:F5O24.140
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length749 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Transglycosidase operating by a ping-pong reaction mechanism. Involved in the synthesis of raffinose, a major soluble carbohydrate in seeds, roots and tubers By similarity.

Catalytic activity

Alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose = myo-inositol + raffinose.

Developmental stage

Expressed in 24 hours imbibed seeds and during leaf senescence. Ref.6

Induction

By oxidative stress and by dark treatment. Down-regulated by sucrose, but not by osmotic treatment. Ref.6 Ref.7

Sequence similarities

Belongs to the glycosyl hydrolases 36 family.

Sequence caution

The sequence AAL90901.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

The sequence AAN18198.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

The sequence BAE99252.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
   Coding sequence diversityAlternative splicing
   Molecular functionGlycosyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functiongalactinol-sucrose galactosyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q8RX87-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 749749Probable galactinol--sucrose galactosyltransferase 6
PRO_0000389259

Experimental info

Sequence conflict3021K → R in BAH19983. Ref.5
Sequence conflict6701M → V in BAD93984. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 24, 2009. Version 2.
Checksum: B8A9DEBB6A2407E7

FASTA74983,116
        10         20         30         40         50         60 
MTIKPAVRIS DGNLIIKNRT ILTGVPDNVI TTSASEAGPV EGVFVGAVFN KEESKHIVPI 

        70         80         90        100        110        120 
GTLRNSRFMS CFRFKLWWMA QRMGEMGRDI PYETQFLLVE SNDGSHLESD GANGVECNQK 

       130        140        150        160        170        180 
VYTVFLPLIE GSFRSCLQGN VNDEVELCLE SGDVDTKRSS FTHSLYIHAG TDPFQTITDA 

       190        200        210        220        230        240 
IRTVKLHLNS FRQRHEKKLP GIVDYFGWCT WDAFYQEVTQ EGVEAGLKSL AAGGTPPKFV 

       250        260        270        280        290        300 
IIDDGWQSVE RDATVEAGDE KKESPIFRLT GIKENEKFKK KDDPNVGIKN IVKIAKEKHG 

       310        320        330        340        350        360 
LKYVYVWHAI TGYWGGVRPG EEYGSVMKYP NMSKGVVEND PTWKTDVMTL QGLGLVSPKK 

       370        380        390        400        410        420 
VYKFYNELHS YLADAGVDGV KVDVQCVLET LGGGLGGRVE LTRQFHQALD SSVAKNFPDN 

       430        440        450        460        470        480 
GCIACMSHNT DALYCSKQAA VIRASDDFYP RDPVSHTIHI ASVAYNSVFL GEFMQPDWDM 

       490        500        510        520        530        540 
FHSVHPAAEY HASARAISGG PLYVSDSPGK HNFELLRKLV LPDGSILRAR LPGRPTRDCL 

       550        560        570        580        590        600 
FADPARDGVS LLKIWNMNKY TGVLGVYNCQ GAAWSSTERK NIFHQTKTDS LTGSIRGRDV 

       610        620        630        640        650        660 
HSISEASTDP TTWNGDCAVY SQSRGELIVM PYNVSLPVSL KIREHEIFTV SPISHLVDGV 

       670        680        690        700        710        720 
SFAPIGLVNM YNSGGAIEGL RYEAEKMKVV MEVKGCGKFG SYSSVKPKRC VVESNEIAFE 

       730        740 
YDSSSGLVTF ELDKMPIENK RFHLIQVEL 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. expand/collapse author list , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[6]"Dark-inducible genes from Arabidopsis thaliana are associated with leaf senescence and repressed by sugars."
Fujiki Y., Yoshikawa Y., Sato T., Inada N., Ito M., Nishida I., Watanabe A.
Physiol. Plantarum 111:345-352(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION BY DARK AND SUCROSE, DEVELOPMENTAL STAGE.
[7]"Galactinol and raffinose constitute a novel function to protect plants from oxidative damage."
Nishizawa A., Yabuta Y., Shigeoka S.
Plant Physiol. 147:1251-1263(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION BY OXIDATIVE STRESS, GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF296825 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED92818.1.
CP002688 Genomic DNA. Translation: AED92819.1.
CP002688 Genomic DNA. Translation: AED92820.1.
AY090237 mRNA. Translation: AAL90901.1. Different initiation.
BT000632 mRNA. Translation: AAN18198.1. Different initiation.
AK227214 mRNA. Translation: BAE99252.1. Different initiation.
AK221808 mRNA. Translation: BAD93984.1.
AK316714 mRNA. Translation: BAH19441.1.
AK317307 mRNA. Translation: BAH19983.1.
AK318923 mRNA. Translation: BAH57038.1.
RefSeqNP_001031910.1. NM_001036833.2.
NP_001190347.1. NM_001203418.1.
NP_197525.1. NM_122032.5.
NP_851044.2. NM_180713.3.
UniGeneAt.25459.
At.74986.

3D structure databases

ProteinModelPortalQ8RX87.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid17423. 3 interactions.
IntActQ8RX87. 2 interactions.

Protein family/group databases

CAZyGH36. Glycoside Hydrolase Family 36.

Proteomic databases

PaxDbQ8RX87.
PRIDEQ8RX87.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G20250.1; AT5G20250.1; AT5G20250. [Q8RX87-1]
AT5G20250.2; AT5G20250.2; AT5G20250. [Q8RX87-1]
AT5G20250.3; AT5G20250.3; AT5G20250. [Q8RX87-1]
GeneID832147.
KEGGath:AT5G20250.

Organism-specific databases

TAIRAT5G20250.

Phylogenomic databases

eggNOGNOG06986.
HOGENOMHOG000237551.
InParanoidQ8RX87.
KOK06617.
PhylomeDBQ8RX87.
ProtClustDBPLN02684.

Enzyme and pathway databases

BioCycARA:AT5G20250-MONOMER.
ARA:GQT-1883-MONOMER.
ARA:GQT-1884-MONOMER.
ARA:GQT-1885-MONOMER.

Family and domain databases

Gene3D3.20.20.70. 2 hits.
InterProIPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR008811. Glycosyl_hydrolases_36.
[Graphical view]
PfamPF05691. Raffinose_syn. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 2 hits.
ProtoNetSearch...

Entry information

Entry nameRFS6_ARATH
AccessionPrimary (citable) accession number: Q8RX87
Secondary accession number(s): B9DFC4, B9DGW6, Q56X69
Entry history
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: November 24, 2009
Last modified: April 16, 2014
This is version 72 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names