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Protein

Probable galactinol--sucrose galactosyltransferase 6

Gene

RFS6

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Transglycosidase operating by a ping-pong reaction mechanism. Involved in the synthesis of raffinose, a major soluble carbohydrate in seeds, roots and tubers (By similarity).By similarity

Catalytic activityi

Alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose = myo-inositol + raffinose.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processCarbohydrate metabolism

Enzyme and pathway databases

BioCyciARA:AT5G20250-MONOMER
BRENDAi2.4.1.82 399

Protein family/group databases

CAZyiGH36 Glycoside Hydrolase Family 36

Names & Taxonomyi

Protein namesi
Recommended name:
Probable galactinol--sucrose galactosyltransferase 6 (EC:2.4.1.82)
Alternative name(s):
Protein DARK INDUCIBLE 10
Raffinose synthase 6
Gene namesi
Name:RFS6
Synonyms:DIN10, RS6
Ordered Locus Names:At5g20250
ORF Names:F5O24.140
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G20250

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003892591 – 749Probable galactinol--sucrose galactosyltransferase 6Add BLAST749

Proteomic databases

PaxDbiQ8RX87
PRIDEiQ8RX87

PTM databases

iPTMnetiQ8RX87

Expressioni

Developmental stagei

Expressed in 24 hours imbibed seeds and during leaf senescence.1 Publication

Inductioni

By oxidative stress and by dark treatment. Down-regulated by sucrose, but not by osmotic treatment.2 Publications

Gene expression databases

ExpressionAtlasiQ8RX87 baseline and differential
GenevisibleiQ8RX87 AT

Interactioni

Protein-protein interaction databases

BioGridi17423, 3 interactors
IntActiQ8RX87, 2 interactors
STRINGi3702.AT5G20250.4

Structurei

3D structure databases

ProteinModelPortaliQ8RX87
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolases 36 family.Curated

Phylogenomic databases

eggNOGiENOG410IH7P Eukaryota
ENOG410YBU9 LUCA
HOGENOMiHOG000237551
InParanoidiQ8RX87
KOiK06617
PhylomeDBiQ8RX87

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR017853 Glycoside_hydrolase_SF
IPR008811 Glycosyl_hydrolases_36
PANTHERiPTHR31268 PTHR31268, 1 hit
PfamiView protein in Pfam
PF05691 Raffinose_syn, 1 hit
SUPFAMiSSF51445 SSF51445, 2 hits

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q8RX87-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTIKPAVRIS DGNLIIKNRT ILTGVPDNVI TTSASEAGPV EGVFVGAVFN
60 70 80 90 100
KEESKHIVPI GTLRNSRFMS CFRFKLWWMA QRMGEMGRDI PYETQFLLVE
110 120 130 140 150
SNDGSHLESD GANGVECNQK VYTVFLPLIE GSFRSCLQGN VNDEVELCLE
160 170 180 190 200
SGDVDTKRSS FTHSLYIHAG TDPFQTITDA IRTVKLHLNS FRQRHEKKLP
210 220 230 240 250
GIVDYFGWCT WDAFYQEVTQ EGVEAGLKSL AAGGTPPKFV IIDDGWQSVE
260 270 280 290 300
RDATVEAGDE KKESPIFRLT GIKENEKFKK KDDPNVGIKN IVKIAKEKHG
310 320 330 340 350
LKYVYVWHAI TGYWGGVRPG EEYGSVMKYP NMSKGVVEND PTWKTDVMTL
360 370 380 390 400
QGLGLVSPKK VYKFYNELHS YLADAGVDGV KVDVQCVLET LGGGLGGRVE
410 420 430 440 450
LTRQFHQALD SSVAKNFPDN GCIACMSHNT DALYCSKQAA VIRASDDFYP
460 470 480 490 500
RDPVSHTIHI ASVAYNSVFL GEFMQPDWDM FHSVHPAAEY HASARAISGG
510 520 530 540 550
PLYVSDSPGK HNFELLRKLV LPDGSILRAR LPGRPTRDCL FADPARDGVS
560 570 580 590 600
LLKIWNMNKY TGVLGVYNCQ GAAWSSTERK NIFHQTKTDS LTGSIRGRDV
610 620 630 640 650
HSISEASTDP TTWNGDCAVY SQSRGELIVM PYNVSLPVSL KIREHEIFTV
660 670 680 690 700
SPISHLVDGV SFAPIGLVNM YNSGGAIEGL RYEAEKMKVV MEVKGCGKFG
710 720 730 740
SYSSVKPKRC VVESNEIAFE YDSSSGLVTF ELDKMPIENK RFHLIQVEL
Length:749
Mass (Da):83,116
Last modified:November 24, 2009 - v2
Checksum:iB8A9DEBB6A2407E7
GO

Sequence cautioni

The sequence AAL90901 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAN18198 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAE99252 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti302K → R in BAH19983 (PubMed:19423640).Curated1
Sequence conflicti670M → V in BAD93984 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF296825 Genomic DNA No translation available.
CP002688 Genomic DNA Translation: AED92818.1
CP002688 Genomic DNA Translation: AED92819.1
CP002688 Genomic DNA Translation: AED92820.1
AY090237 mRNA Translation: AAL90901.1 Different initiation.
BT000632 mRNA Translation: AAN18198.1 Different initiation.
AK227214 mRNA Translation: BAE99252.1 Different initiation.
AK221808 mRNA Translation: BAD93984.1
AK316714 mRNA Translation: BAH19441.1
AK317307 mRNA Translation: BAH19983.1
AK318923 mRNA Translation: BAH57038.1
RefSeqiNP_001031910.1, NM_001036833.2 [Q8RX87-1]
NP_001190347.1, NM_001203418.1
NP_197525.1, NM_122032.6 [Q8RX87-1]
NP_851044.2, NM_180713.4 [Q8RX87-1]
UniGeneiAt.25459
At.74986

Genome annotation databases

EnsemblPlantsiAT5G20250.1; AT5G20250.1; AT5G20250 [Q8RX87-1]
AT5G20250.2; AT5G20250.2; AT5G20250 [Q8RX87-1]
AT5G20250.3; AT5G20250.3; AT5G20250 [Q8RX87-1]
GeneIDi832147
GrameneiAT5G20250.1; AT5G20250.1; AT5G20250 [Q8RX87-1]
AT5G20250.2; AT5G20250.2; AT5G20250 [Q8RX87-1]
AT5G20250.3; AT5G20250.3; AT5G20250 [Q8RX87-1]
KEGGiath:AT5G20250

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiRFS6_ARATH
AccessioniPrimary (citable) accession number: Q8RX87
Secondary accession number(s): B9DFC4, B9DGW6, Q56X69
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: November 24, 2009
Last modified: April 25, 2018
This is version 95 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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