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Q8RX87

- RFS6_ARATH

UniProt

Q8RX87 - RFS6_ARATH

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Protein

Probable galactinol--sucrose galactosyltransferase 6

Gene

RFS6

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Transglycosidase operating by a ping-pong reaction mechanism. Involved in the synthesis of raffinose, a major soluble carbohydrate in seeds, roots and tubers By similarity.By similarity

Catalytic activityi

Alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose = myo-inositol + raffinose.

GO - Molecular functioni

  1. galactinol-sucrose galactosyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism

Enzyme and pathway databases

BioCyciARA:AT5G20250-MONOMER.
ARA:GQT-1883-MONOMER.
ARA:GQT-1884-MONOMER.
ARA:GQT-1885-MONOMER.

Protein family/group databases

CAZyiGH36. Glycoside Hydrolase Family 36.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable galactinol--sucrose galactosyltransferase 6 (EC:2.4.1.82)
Alternative name(s):
Protein DARK INDUCIBLE 10
Raffinose synthase 6
Gene namesi
Name:RFS6
Synonyms:DIN10, RS6
Ordered Locus Names:At5g20250
ORF Names:F5O24.140
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G20250.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 749749Probable galactinol--sucrose galactosyltransferase 6PRO_0000389259Add
BLAST

Proteomic databases

PaxDbiQ8RX87.
PRIDEiQ8RX87.

Expressioni

Developmental stagei

Expressed in 24 hours imbibed seeds and during leaf senescence.1 Publication

Inductioni

By oxidative stress and by dark treatment. Down-regulated by sucrose, but not by osmotic treatment.2 Publications

Interactioni

Protein-protein interaction databases

BioGridi17423. 3 interactions.
IntActiQ8RX87. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ8RX87.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolases 36 family.Curated

Phylogenomic databases

eggNOGiNOG06986.
HOGENOMiHOG000237551.
InParanoidiQ8RX87.
KOiK06617.
PhylomeDBiQ8RX87.

Family and domain databases

Gene3Di3.20.20.70. 2 hits.
InterProiIPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR008811. Glycosyl_hydrolases_36.
[Graphical view]
PfamiPF05691. Raffinose_syn. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 2 hits.

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q8RX87-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTIKPAVRIS DGNLIIKNRT ILTGVPDNVI TTSASEAGPV EGVFVGAVFN
60 70 80 90 100
KEESKHIVPI GTLRNSRFMS CFRFKLWWMA QRMGEMGRDI PYETQFLLVE
110 120 130 140 150
SNDGSHLESD GANGVECNQK VYTVFLPLIE GSFRSCLQGN VNDEVELCLE
160 170 180 190 200
SGDVDTKRSS FTHSLYIHAG TDPFQTITDA IRTVKLHLNS FRQRHEKKLP
210 220 230 240 250
GIVDYFGWCT WDAFYQEVTQ EGVEAGLKSL AAGGTPPKFV IIDDGWQSVE
260 270 280 290 300
RDATVEAGDE KKESPIFRLT GIKENEKFKK KDDPNVGIKN IVKIAKEKHG
310 320 330 340 350
LKYVYVWHAI TGYWGGVRPG EEYGSVMKYP NMSKGVVEND PTWKTDVMTL
360 370 380 390 400
QGLGLVSPKK VYKFYNELHS YLADAGVDGV KVDVQCVLET LGGGLGGRVE
410 420 430 440 450
LTRQFHQALD SSVAKNFPDN GCIACMSHNT DALYCSKQAA VIRASDDFYP
460 470 480 490 500
RDPVSHTIHI ASVAYNSVFL GEFMQPDWDM FHSVHPAAEY HASARAISGG
510 520 530 540 550
PLYVSDSPGK HNFELLRKLV LPDGSILRAR LPGRPTRDCL FADPARDGVS
560 570 580 590 600
LLKIWNMNKY TGVLGVYNCQ GAAWSSTERK NIFHQTKTDS LTGSIRGRDV
610 620 630 640 650
HSISEASTDP TTWNGDCAVY SQSRGELIVM PYNVSLPVSL KIREHEIFTV
660 670 680 690 700
SPISHLVDGV SFAPIGLVNM YNSGGAIEGL RYEAEKMKVV MEVKGCGKFG
710 720 730 740
SYSSVKPKRC VVESNEIAFE YDSSSGLVTF ELDKMPIENK RFHLIQVEL
Length:749
Mass (Da):83,116
Last modified:November 24, 2009 - v2
Checksum:iB8A9DEBB6A2407E7
GO

Sequence cautioni

The sequence AAL90901.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
The sequence AAN18198.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
The sequence BAE99252.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti302 – 3021K → R in BAH19983. (PubMed:19423640)Curated
Sequence conflicti670 – 6701M → V in BAD93984. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF296825 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED92818.1.
CP002688 Genomic DNA. Translation: AED92819.1.
CP002688 Genomic DNA. Translation: AED92820.1.
AY090237 mRNA. Translation: AAL90901.1. Different initiation.
BT000632 mRNA. Translation: AAN18198.1. Different initiation.
AK227214 mRNA. Translation: BAE99252.1. Different initiation.
AK221808 mRNA. Translation: BAD93984.1.
AK316714 mRNA. Translation: BAH19441.1.
AK317307 mRNA. Translation: BAH19983.1.
AK318923 mRNA. Translation: BAH57038.1.
RefSeqiNP_001031910.1. NM_001036833.2. [Q8RX87-1]
NP_001190347.1. NM_001203418.1.
NP_197525.1. NM_122032.5. [Q8RX87-1]
NP_851044.2. NM_180713.3. [Q8RX87-1]
UniGeneiAt.25459.
At.74986.

Genome annotation databases

EnsemblPlantsiAT5G20250.1; AT5G20250.1; AT5G20250. [Q8RX87-1]
AT5G20250.2; AT5G20250.2; AT5G20250. [Q8RX87-1]
AT5G20250.3; AT5G20250.3; AT5G20250. [Q8RX87-1]
AT5G20250.4; AT5G20250.4; AT5G20250.
GeneIDi832147.
KEGGiath:AT5G20250.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF296825 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED92818.1 .
CP002688 Genomic DNA. Translation: AED92819.1 .
CP002688 Genomic DNA. Translation: AED92820.1 .
AY090237 mRNA. Translation: AAL90901.1 . Different initiation.
BT000632 mRNA. Translation: AAN18198.1 . Different initiation.
AK227214 mRNA. Translation: BAE99252.1 . Different initiation.
AK221808 mRNA. Translation: BAD93984.1 .
AK316714 mRNA. Translation: BAH19441.1 .
AK317307 mRNA. Translation: BAH19983.1 .
AK318923 mRNA. Translation: BAH57038.1 .
RefSeqi NP_001031910.1. NM_001036833.2. [Q8RX87-1 ]
NP_001190347.1. NM_001203418.1.
NP_197525.1. NM_122032.5. [Q8RX87-1 ]
NP_851044.2. NM_180713.3. [Q8RX87-1 ]
UniGenei At.25459.
At.74986.

3D structure databases

ProteinModelPortali Q8RX87.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 17423. 3 interactions.
IntActi Q8RX87. 2 interactions.

Protein family/group databases

CAZyi GH36. Glycoside Hydrolase Family 36.

Proteomic databases

PaxDbi Q8RX87.
PRIDEi Q8RX87.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G20250.1 ; AT5G20250.1 ; AT5G20250 . [Q8RX87-1 ]
AT5G20250.2 ; AT5G20250.2 ; AT5G20250 . [Q8RX87-1 ]
AT5G20250.3 ; AT5G20250.3 ; AT5G20250 . [Q8RX87-1 ]
AT5G20250.4 ; AT5G20250.4 ; AT5G20250 .
GeneIDi 832147.
KEGGi ath:AT5G20250.

Organism-specific databases

TAIRi AT5G20250.

Phylogenomic databases

eggNOGi NOG06986.
HOGENOMi HOG000237551.
InParanoidi Q8RX87.
KOi K06617.
PhylomeDBi Q8RX87.

Enzyme and pathway databases

BioCyci ARA:AT5G20250-MONOMER.
ARA:GQT-1883-MONOMER.
ARA:GQT-1884-MONOMER.
ARA:GQT-1885-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 2 hits.
InterProi IPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR008811. Glycosyl_hydrolases_36.
[Graphical view ]
Pfami PF05691. Raffinose_syn. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 2 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
    Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
    , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
    Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
    Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
    DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Dark-inducible genes from Arabidopsis thaliana are associated with leaf senescence and repressed by sugars."
    Fujiki Y., Yoshikawa Y., Sato T., Inada N., Ito M., Nishida I., Watanabe A.
    Physiol. Plantarum 111:345-352(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY DARK AND SUCROSE, DEVELOPMENTAL STAGE.
  7. "Galactinol and raffinose constitute a novel function to protect plants from oxidative damage."
    Nishizawa A., Yabuta Y., Shigeoka S.
    Plant Physiol. 147:1251-1263(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY OXIDATIVE STRESS, GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiRFS6_ARATH
AccessioniPrimary (citable) accession number: Q8RX87
Secondary accession number(s): B9DFC4, B9DGW6, Q56X69
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: November 24, 2009
Last modified: October 1, 2014
This is version 75 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3