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Q8RX87

- RFS6_ARATH

UniProt

Q8RX87 - RFS6_ARATH

Protein

Probable galactinol--sucrose galactosyltransferase 6

Gene

RFS6

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 2 (24 Nov 2009)
      Previous versions | rss
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    Functioni

    Transglycosidase operating by a ping-pong reaction mechanism. Involved in the synthesis of raffinose, a major soluble carbohydrate in seeds, roots and tubers By similarity.By similarity

    Catalytic activityi

    Alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose = myo-inositol + raffinose.

    GO - Molecular functioni

    1. galactinol-sucrose galactosyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Carbohydrate metabolism

    Enzyme and pathway databases

    BioCyciARA:AT5G20250-MONOMER.
    ARA:GQT-1883-MONOMER.
    ARA:GQT-1884-MONOMER.
    ARA:GQT-1885-MONOMER.

    Protein family/group databases

    CAZyiGH36. Glycoside Hydrolase Family 36.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable galactinol--sucrose galactosyltransferase 6 (EC:2.4.1.82)
    Alternative name(s):
    Protein DARK INDUCIBLE 10
    Raffinose synthase 6
    Gene namesi
    Name:RFS6
    Synonyms:DIN10, RS6
    Ordered Locus Names:At5g20250
    ORF Names:F5O24.140
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G20250.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 749749Probable galactinol--sucrose galactosyltransferase 6PRO_0000389259Add
    BLAST

    Proteomic databases

    PaxDbiQ8RX87.
    PRIDEiQ8RX87.

    Expressioni

    Developmental stagei

    Expressed in 24 hours imbibed seeds and during leaf senescence.1 Publication

    Inductioni

    By oxidative stress and by dark treatment. Down-regulated by sucrose, but not by osmotic treatment.2 Publications

    Interactioni

    Protein-protein interaction databases

    BioGridi17423. 3 interactions.
    IntActiQ8RX87. 2 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8RX87.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolases 36 family.Curated

    Phylogenomic databases

    eggNOGiNOG06986.
    HOGENOMiHOG000237551.
    InParanoidiQ8RX87.
    KOiK06617.
    PhylomeDBiQ8RX87.

    Family and domain databases

    Gene3Di3.20.20.70. 2 hits.
    InterProiIPR013785. Aldolase_TIM.
    IPR017853. Glycoside_hydrolase_SF.
    IPR008811. Glycosyl_hydrolases_36.
    [Graphical view]
    PfamiPF05691. Raffinose_syn. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: Q8RX87-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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    MTIKPAVRIS DGNLIIKNRT ILTGVPDNVI TTSASEAGPV EGVFVGAVFN    50
    KEESKHIVPI GTLRNSRFMS CFRFKLWWMA QRMGEMGRDI PYETQFLLVE 100
    SNDGSHLESD GANGVECNQK VYTVFLPLIE GSFRSCLQGN VNDEVELCLE 150
    SGDVDTKRSS FTHSLYIHAG TDPFQTITDA IRTVKLHLNS FRQRHEKKLP 200
    GIVDYFGWCT WDAFYQEVTQ EGVEAGLKSL AAGGTPPKFV IIDDGWQSVE 250
    RDATVEAGDE KKESPIFRLT GIKENEKFKK KDDPNVGIKN IVKIAKEKHG 300
    LKYVYVWHAI TGYWGGVRPG EEYGSVMKYP NMSKGVVEND PTWKTDVMTL 350
    QGLGLVSPKK VYKFYNELHS YLADAGVDGV KVDVQCVLET LGGGLGGRVE 400
    LTRQFHQALD SSVAKNFPDN GCIACMSHNT DALYCSKQAA VIRASDDFYP 450
    RDPVSHTIHI ASVAYNSVFL GEFMQPDWDM FHSVHPAAEY HASARAISGG 500
    PLYVSDSPGK HNFELLRKLV LPDGSILRAR LPGRPTRDCL FADPARDGVS 550
    LLKIWNMNKY TGVLGVYNCQ GAAWSSTERK NIFHQTKTDS LTGSIRGRDV 600
    HSISEASTDP TTWNGDCAVY SQSRGELIVM PYNVSLPVSL KIREHEIFTV 650
    SPISHLVDGV SFAPIGLVNM YNSGGAIEGL RYEAEKMKVV MEVKGCGKFG 700
    SYSSVKPKRC VVESNEIAFE YDSSSGLVTF ELDKMPIENK RFHLIQVEL 749
    Length:749
    Mass (Da):83,116
    Last modified:November 24, 2009 - v2
    Checksum:iB8A9DEBB6A2407E7
    GO

    Sequence cautioni

    The sequence AAL90901.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
    The sequence AAN18198.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
    The sequence BAE99252.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti302 – 3021K → R in BAH19983. (PubMed:19423640)Curated
    Sequence conflicti670 – 6701M → V in BAD93984. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF296825 Genomic DNA. No translation available.
    CP002688 Genomic DNA. Translation: AED92818.1.
    CP002688 Genomic DNA. Translation: AED92819.1.
    CP002688 Genomic DNA. Translation: AED92820.1.
    AY090237 mRNA. Translation: AAL90901.1. Different initiation.
    BT000632 mRNA. Translation: AAN18198.1. Different initiation.
    AK227214 mRNA. Translation: BAE99252.1. Different initiation.
    AK221808 mRNA. Translation: BAD93984.1.
    AK316714 mRNA. Translation: BAH19441.1.
    AK317307 mRNA. Translation: BAH19983.1.
    AK318923 mRNA. Translation: BAH57038.1.
    RefSeqiNP_001031910.1. NM_001036833.2. [Q8RX87-1]
    NP_001190347.1. NM_001203418.1.
    NP_197525.1. NM_122032.5. [Q8RX87-1]
    NP_851044.2. NM_180713.3. [Q8RX87-1]
    UniGeneiAt.25459.
    At.74986.

    Genome annotation databases

    EnsemblPlantsiAT5G20250.1; AT5G20250.1; AT5G20250. [Q8RX87-1]
    AT5G20250.2; AT5G20250.2; AT5G20250. [Q8RX87-1]
    AT5G20250.3; AT5G20250.3; AT5G20250. [Q8RX87-1]
    AT5G20250.4; AT5G20250.4; AT5G20250.
    GeneIDi832147.
    KEGGiath:AT5G20250.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF296825 Genomic DNA. No translation available.
    CP002688 Genomic DNA. Translation: AED92818.1 .
    CP002688 Genomic DNA. Translation: AED92819.1 .
    CP002688 Genomic DNA. Translation: AED92820.1 .
    AY090237 mRNA. Translation: AAL90901.1 . Different initiation.
    BT000632 mRNA. Translation: AAN18198.1 . Different initiation.
    AK227214 mRNA. Translation: BAE99252.1 . Different initiation.
    AK221808 mRNA. Translation: BAD93984.1 .
    AK316714 mRNA. Translation: BAH19441.1 .
    AK317307 mRNA. Translation: BAH19983.1 .
    AK318923 mRNA. Translation: BAH57038.1 .
    RefSeqi NP_001031910.1. NM_001036833.2. [Q8RX87-1 ]
    NP_001190347.1. NM_001203418.1.
    NP_197525.1. NM_122032.5. [Q8RX87-1 ]
    NP_851044.2. NM_180713.3. [Q8RX87-1 ]
    UniGenei At.25459.
    At.74986.

    3D structure databases

    ProteinModelPortali Q8RX87.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 17423. 3 interactions.
    IntActi Q8RX87. 2 interactions.

    Protein family/group databases

    CAZyi GH36. Glycoside Hydrolase Family 36.

    Proteomic databases

    PaxDbi Q8RX87.
    PRIDEi Q8RX87.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G20250.1 ; AT5G20250.1 ; AT5G20250 . [Q8RX87-1 ]
    AT5G20250.2 ; AT5G20250.2 ; AT5G20250 . [Q8RX87-1 ]
    AT5G20250.3 ; AT5G20250.3 ; AT5G20250 . [Q8RX87-1 ]
    AT5G20250.4 ; AT5G20250.4 ; AT5G20250 .
    GeneIDi 832147.
    KEGGi ath:AT5G20250.

    Organism-specific databases

    TAIRi AT5G20250.

    Phylogenomic databases

    eggNOGi NOG06986.
    HOGENOMi HOG000237551.
    InParanoidi Q8RX87.
    KOi K06617.
    PhylomeDBi Q8RX87.

    Enzyme and pathway databases

    BioCyci ARA:AT5G20250-MONOMER.
    ARA:GQT-1883-MONOMER.
    ARA:GQT-1884-MONOMER.
    ARA:GQT-1885-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 2 hits.
    InterProi IPR013785. Aldolase_TIM.
    IPR017853. Glycoside_hydrolase_SF.
    IPR008811. Glycosyl_hydrolases_36.
    [Graphical view ]
    Pfami PF05691. Raffinose_syn. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
      Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
      , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
      Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
      Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
      DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "Dark-inducible genes from Arabidopsis thaliana are associated with leaf senescence and repressed by sugars."
      Fujiki Y., Yoshikawa Y., Sato T., Inada N., Ito M., Nishida I., Watanabe A.
      Physiol. Plantarum 111:345-352(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION BY DARK AND SUCROSE, DEVELOPMENTAL STAGE.
    7. "Galactinol and raffinose constitute a novel function to protect plants from oxidative damage."
      Nishizawa A., Yabuta Y., Shigeoka S.
      Plant Physiol. 147:1251-1263(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION BY OXIDATIVE STRESS, GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiRFS6_ARATH
    AccessioniPrimary (citable) accession number: Q8RX87
    Secondary accession number(s): B9DFC4, B9DGW6, Q56X69
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 24, 2009
    Last sequence update: November 24, 2009
    Last modified: October 1, 2014
    This is version 75 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3