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Protein

Alpha-galactosidase 2

Gene

AGAL2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May regulate leaf (and possibly other organ) development by functioning in cell wall loosening and cell wall expansion.1 Publication

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei159SubstrateBy similarity1
Active sitei161NucleophileBy similarity1
Binding sitei212SubstrateBy similarity1
Active sitei216Proton donorBy similarity1
Binding sitei216SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • carbohydrate metabolic process Source: InterPro
  • cell wall organization Source: UniProtKB-KW
  • leaf morphogenesis Source: TAIR
  • positive regulation of flower development Source: TAIR
  • response to fungus Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciARA:AT5G08370-MONOMER.
ReactomeiR-ATH-1660662. Glycosphingolipid metabolism.
R-ATH-6798695. Neutrophil degranulation.

Protein family/group databases

CAZyiGH27. Glycoside Hydrolase Family 27.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-galactosidase 21 Publication (EC:3.2.1.22By similarity)
Short name:
AtAGAL21 Publication
Alternative name(s):
Alpha-D-galactoside galactohydrolase 2Curated
MelibiaseCurated
Gene namesi
Name:AGAL21 Publication
Ordered Locus Names:At5g08370Imported
ORF Names:F8L15.100Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)Imported
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G08370.

Subcellular locationi

GO - Cellular componenti

  • apoplast Source: UniProtKB
  • plant-type cell wall Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Cell wall, Secreted

Pathology & Biotechi

Disruption phenotypei

Increased rosette leaves number with a curly surface leaf morphology and delayed flowering.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000043184626 – 396Alpha-galactosidase 2Sequence analysisAdd BLAST371

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi52 ↔ 84By similarity
Glycosylationi55N-linked (GlcNAc...)PROSITE-ProRule annotation1
Disulfide bondi132 ↔ 163By similarity
Glycosylationi343N-linked (GlcNAc...)PROSITE-ProRule annotation1
Glycosylationi354N-linked (GlcNAc...)PROSITE-ProRule annotation1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8RX86.
PRIDEiQ8RX86.

PTM databases

iPTMnetiQ8RX86.

Expressioni

Inductioni

By Verticillium longisporum (VL43), in apoplasm.1 Publication

Gene expression databases

ExpressionAtlasiQ8RX86. baseline and differential.
GenevisibleiQ8RX86. AT.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi3702.AT5G08370.1.

Structurei

3D structure databases

ProteinModelPortaliQ8RX86.
SMRiQ8RX86.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni82 – 83Substrate bindingBy similarity2
Regioni194 – 198Substrate bindingBy similarity5

Sequence similaritiesi

Belongs to the glycosyl hydrolase 27 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2366. Eukaryota.
ENOG410XPF1. LUCA.
HOGENOMiHOG000161224.
KOiK07407.
OMAiKENPATW.
OrthoDBiEOG09360A7S.
PhylomeDBiQ8RX86.

Family and domain databases

CDDicd14792. GH27. 1 hit.
Gene3Di2.60.40.1180. 1 hit.
3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR002241. Glyco_hydro_27.
IPR000111. Glyco_hydro_27/36_CS.
IPR013780. Glyco_hydro_b.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF16499. Melibiase_2. 1 hit.
[Graphical view]
PRINTSiPR00740. GLHYDRLASE27.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00512. ALPHA_GALACTOSIDASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8RX86-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVLLSFSLRF IAFTLTITLT QIADGFQSRM LMNNGLALSP QMGWNSWNHF
60 70 80 90 100
QCNINETLIK QTADAMVSSG LSAIGYKYIN IDDCWGELKR DSQGSLVAKA
110 120 130 140 150
STFPSGIKAL SDYVHSKGLK LGIYSDAGTL TCSQTMPGSL GHEEQDAKTF
160 170 180 190 200
ASWGIDYLKY DNCENTGTSP RERYPKMSKA LLNSGRSIFF SLCEWGQEDP
210 220 230 240 250
ATWAGDIGNS WRTTGDIQDN WKSMTLIADQ NDRWASYARP GSWNDPDMLE
260 270 280 290 300
VGNGGMTKEE YMSHFSIWAL AKAPLLIGCD LRSMDKVTFE LLSNKEVIAV
310 320 330 340 350
NQDKLGIQGK KVKKEGDLEV WAGPLSKKRV AVILWNRGSA SANITARWAE
360 370 380 390
IGLNSSDIVN ARDLWEHSTY SCVKKQLSAL VEPHACKMYT LTRRKA
Length:396
Mass (Da):44,037
Last modified:June 1, 2002 - v1
Checksum:i6859823801D235B6
GO
Isoform 2 (identifier: Q8RX86-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-27: MVLLSFSLRFIAFTLTITLTQIADGFQ → M

Note: No experimental confirmation available.Imported
Show »
Length:370
Mass (Da):41,139
Checksum:i5A3798502B96A1AF
GO

Sequence cautioni

The sequence CAC08337 differs from that shown. Reason: Erroneous gene model prediction.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0574491 – 27MVLLS…ADGFQ → M in isoform 2. Add BLAST27

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL392174 Genomic DNA. Translation: CAC08337.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED91290.1.
CP002688 Genomic DNA. Translation: AED91291.1.
AY090238 mRNA. Translation: AAL90902.1.
BT000619 mRNA. Translation: AAN18186.1.
AK317201 mRNA. Translation: BAH19885.1.
AY085529 mRNA. Translation: AAM62753.1.
RefSeqiNP_001031855.1. NM_001036778.2. [Q8RX86-2]
NP_568193.1. NM_120921.5. [Q8RX86-1]
UniGeneiAt.32587.
At.48997.

Genome annotation databases

EnsemblPlantsiAT5G08370.1; AT5G08370.1; AT5G08370. [Q8RX86-1]
AT5G08370.2; AT5G08370.2; AT5G08370. [Q8RX86-2]
GeneIDi830735.
GrameneiAT5G08370.1; AT5G08370.1; AT5G08370.
AT5G08370.2; AT5G08370.2; AT5G08370.
KEGGiath:AT5G08370.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL392174 Genomic DNA. Translation: CAC08337.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED91290.1.
CP002688 Genomic DNA. Translation: AED91291.1.
AY090238 mRNA. Translation: AAL90902.1.
BT000619 mRNA. Translation: AAN18186.1.
AK317201 mRNA. Translation: BAH19885.1.
AY085529 mRNA. Translation: AAM62753.1.
RefSeqiNP_001031855.1. NM_001036778.2. [Q8RX86-2]
NP_568193.1. NM_120921.5. [Q8RX86-1]
UniGeneiAt.32587.
At.48997.

3D structure databases

ProteinModelPortaliQ8RX86.
SMRiQ8RX86.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G08370.1.

Protein family/group databases

CAZyiGH27. Glycoside Hydrolase Family 27.

PTM databases

iPTMnetiQ8RX86.

Proteomic databases

PaxDbiQ8RX86.
PRIDEiQ8RX86.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G08370.1; AT5G08370.1; AT5G08370. [Q8RX86-1]
AT5G08370.2; AT5G08370.2; AT5G08370. [Q8RX86-2]
GeneIDi830735.
GrameneiAT5G08370.1; AT5G08370.1; AT5G08370.
AT5G08370.2; AT5G08370.2; AT5G08370.
KEGGiath:AT5G08370.

Organism-specific databases

TAIRiAT5G08370.

Phylogenomic databases

eggNOGiKOG2366. Eukaryota.
ENOG410XPF1. LUCA.
HOGENOMiHOG000161224.
KOiK07407.
OMAiKENPATW.
OrthoDBiEOG09360A7S.
PhylomeDBiQ8RX86.

Enzyme and pathway databases

BioCyciARA:AT5G08370-MONOMER.
ReactomeiR-ATH-1660662. Glycosphingolipid metabolism.
R-ATH-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiQ8RX86.

Gene expression databases

ExpressionAtlasiQ8RX86. baseline and differential.
GenevisibleiQ8RX86. AT.

Family and domain databases

CDDicd14792. GH27. 1 hit.
Gene3Di2.60.40.1180. 1 hit.
3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR002241. Glyco_hydro_27.
IPR000111. Glyco_hydro_27/36_CS.
IPR013780. Glyco_hydro_b.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF16499. Melibiase_2. 1 hit.
[Graphical view]
PRINTSiPR00740. GLHYDRLASE27.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00512. ALPHA_GALACTOSIDASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAGAL2_ARATH
AccessioniPrimary (citable) accession number: Q8RX86
Secondary accession number(s): B9DGL8, Q9FT98
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 4, 2015
Last sequence update: June 1, 2002
Last modified: November 30, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.