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Protein

Protein NRT1/ PTR FAMILY 2.13

Gene

NPF2.13

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Low-affinity proton-dependent nitrate transporter. Not involved in dipeptides transport, but has a weak glucosinolate transport activity. Involved in phloem loading and nitrate remobilization from the older leaves to other tissues.2 Publications

Kineticsi

  1. KM=2.8 mM for nitrate at -60 mV1 Publication

    GO - Molecular functioni

    • low-affinity nitrate transmembrane transporter activity Source: TAIR
    • symporter activity Source: UniProtKB-KW

    GO - Biological processi

    • nitrate assimilation Source: UniProtKB-KW
    • nitrate transport Source: TAIR

    Keywordsi

    Biological processNitrate assimilation, Symport, Transport

    Enzyme and pathway databases

    ReactomeiR-ATH-427975 Proton/oligopeptide cotransporters
    R-ATH-6798695 Neutrophil degranulation

    Protein family/group databases

    TCDBi2.A.17.3.15 the proton-dependent oligopeptide transporter (pot/ptr) family

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein NRT1/ PTR FAMILY 2.13
    Short name:
    AtNPF2.13
    Alternative name(s):
    Nitrate transporter 1.7
    Gene namesi
    Name:NPF2.13
    Synonyms:NRT1.7
    Ordered Locus Names:At1g69870
    ORF Names:T17F3.10
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 1

    Organism-specific databases

    AraportiAT1G69870
    TAIRilocus:2196800 AT1G69870

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transmembranei70 – 90HelicalSequence analysisAdd BLAST21
    Transmembranei95 – 115HelicalSequence analysisAdd BLAST21
    Transmembranei126 – 146HelicalSequence analysisAdd BLAST21
    Transmembranei167 – 187HelicalSequence analysisAdd BLAST21
    Transmembranei213 – 233HelicalSequence analysisAdd BLAST21
    Transmembranei241 – 261HelicalSequence analysisAdd BLAST21
    Transmembranei364 – 384HelicalSequence analysisAdd BLAST21
    Transmembranei402 – 422HelicalSequence analysisAdd BLAST21
    Transmembranei443 – 463HelicalSequence analysisAdd BLAST21
    Transmembranei485 – 505HelicalSequence analysisAdd BLAST21
    Transmembranei524 – 544HelicalSequence analysisAdd BLAST21
    Transmembranei568 – 588HelicalSequence analysisAdd BLAST21

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype when grown under normal conditions. Growth retardation when starved of nitrogen.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00003999551 – 620Protein NRT1/ PTR FAMILY 2.13Add BLAST620

    Proteomic databases

    PaxDbiQ8RX77
    PRIDEiQ8RX77

    PTM databases

    iPTMnetiQ8RX77

    Expressioni

    Tissue specificityi

    Expressed in leaves and flowers. Detected in stems and siliques. Highest expression in the distal lamina of older leaves. Restricted to the sieve element and companion cell complex of the minor vein.2 Publications

    Inductioni

    Circadian-regulation. Expression increase during the light phase and decrease during the dark phase.1 Publication

    Gene expression databases

    ExpressionAtlasiQ8RX77 baseline and differential
    GenevisibleiQ8RX77 AT

    Interactioni

    Protein-protein interaction databases

    BioGridi28544, 24 interactors
    STRINGi3702.AT1G69870.1

    Structurei

    3D structure databases

    ProteinModelPortaliQ8RX77
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG1237 Eukaryota
    COG3104 LUCA
    HOGENOMiHOG000237399
    InParanoidiQ8RX77
    OMAiLLANFMV
    OrthoDBiEOG093605YH
    PhylomeDBiQ8RX77

    Family and domain databases

    CDDicd06174 MFS, 1 hit
    InterProiView protein in InterPro
    IPR020846 MFS_dom
    IPR036259 MFS_trans_sf
    IPR000109 POT_fam
    PANTHERiPTHR11654 PTHR11654, 1 hit
    PfamiView protein in Pfam
    PF00854 PTR2, 1 hit
    SUPFAMiSSF103473 SSF103473, 2 hits

    Sequencei

    Sequence statusi: Complete.

    Q8RX77-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MVLEDRKDGS SLPGRSGSFS KSSPSELDVV DPYKRISSPG SILDAEKVEK
    60 70 80 90 100
    KPGGWRAVSF ILGNETLERL GSIGLLANFM VYLTKVFHLE QVDAANVINI
    110 120 130 140 150
    WSGFTNLTPL VGAYISDTYV GRFKTIAFAS FATLLGLITI TLTASFPQLH
    160 170 180 190 200
    PASCNSQDPL SCGGPNKLQI GVLLLGLCFL SVGSGGIRPC SIPFGVDQFD
    210 220 230 240 250
    QRTEEGVKGV ASFFNWYYMT FTVVLIITQT VVVYIQDQVS WIIGFSIPTG
    260 270 280 290 300
    LMALAVVMFF AGMKRYVYVK PEGSIFSGIA QVIVAARKKR KLKLPAEDDG
    310 320 330 340 350
    TVTYYDPAIK SSVLSKLHRS NQFRCLDKAA VVIEGDLTPE GPPADKWRLC
    360 370 380 390 400
    SVQEVEEVKC LIRIVPIWSA GIISLAAMTT QGTFTVSQAL KMDRNLGPKF
    410 420 430 440 450
    EIPAGSLSVI SLLTIGIFLP FYDRVFVPFM RRITGHKSGI TLLQRIGTGI
    460 470 480 490 500
    VFAIFSMIVA GIVERMRRIR SINAGDPTGM TPMSVFWLSP QLILMGLCEA
    510 520 530 540 550
    FNIIGQIEFF NSQFPEHMRS IANSLFSLSF AGSSYLSSFL VTVVHKFSGG
    560 570 580 590 600
    HDRPDWLNKN LNAGKLDYFY YLIAVLGVVN LVYFWYCARG YRYKVGLPIE
    610 620
    DFEEDKSSDD VEMTSKKSMK
    Length:620
    Mass (Da):68,417
    Last modified:June 1, 2002 - v1
    Checksum:i3B554DEDC4C1E829
    GO

    Sequence cautioni

    The sequence AAG52567 differs from that shown. Reason: Erroneous gene model prediction.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AC010675 Genomic DNA Translation: AAG52567.1 Sequence problems.
    CP002684 Genomic DNA Translation: AEE34993.1
    AY090257 mRNA Translation: AAL90918.1
    AY125531 mRNA Translation: AAM78041.1
    PIRiA96721
    RefSeqiNP_564979.1, NM_105655.3
    UniGeneiAt.23012

    Genome annotation databases

    EnsemblPlantsiAT1G69870.1; AT1G69870.1; AT1G69870
    GeneIDi843323
    GrameneiAT1G69870.1; AT1G69870.1; AT1G69870
    KEGGiath:AT1G69870

    Similar proteinsi

    Entry informationi

    Entry nameiPTR21_ARATH
    AccessioniPrimary (citable) accession number: Q8RX77
    Secondary accession number(s): Q9CAS0
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 2, 2010
    Last sequence update: June 1, 2002
    Last modified: April 25, 2018
    This is version 86 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

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