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Protein

Protein NRT1/ PTR FAMILY 2.13

Gene

NPF2.13

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Low-affinity proton-dependent nitrate transporter. Not involved in dipeptides transport, but has a weak glucosinolate transport activity. Involved in phloem loading and nitrate remobilization from the older leaves to other tissues.2 Publications

Kineticsi

  1. KM=2.8 mM for nitrate at -60 mV1 Publication

    GO - Molecular functioni

    • low-affinity nitrate transmembrane transporter activity Source: TAIR
    • symporter activity Source: UniProtKB-KW

    GO - Biological processi

    • low-affinity nitrate transport Source: TAIR
    • nitrate assimilation Source: UniProtKB-KW
    • nitrate transport Source: TAIR
    Complete GO annotation...

    Keywords - Biological processi

    Nitrate assimilation, Symport, Transport

    Enzyme and pathway databases

    ReactomeiR-ATH-427975. Proton/oligopeptide cotransporters.

    Protein family/group databases

    TCDBi2.A.17.3.15. the proton-dependent oligopeptide transporter (pot/ptr) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein NRT1/ PTR FAMILY 2.13
    Short name:
    AtNPF2.13
    Alternative name(s):
    Nitrate transporter 1.7
    Gene namesi
    Name:NPF2.13
    Synonyms:NRT1.7
    Ordered Locus Names:At1g69870
    ORF Names:T17F3.10
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 1

    Organism-specific databases

    TAIRiAT1G69870.

    Subcellular locationi

    • Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei70 – 9021HelicalSequence analysisAdd
    BLAST
    Transmembranei95 – 11521HelicalSequence analysisAdd
    BLAST
    Transmembranei126 – 14621HelicalSequence analysisAdd
    BLAST
    Transmembranei167 – 18721HelicalSequence analysisAdd
    BLAST
    Transmembranei213 – 23321HelicalSequence analysisAdd
    BLAST
    Transmembranei241 – 26121HelicalSequence analysisAdd
    BLAST
    Transmembranei364 – 38421HelicalSequence analysisAdd
    BLAST
    Transmembranei402 – 42221HelicalSequence analysisAdd
    BLAST
    Transmembranei443 – 46321HelicalSequence analysisAdd
    BLAST
    Transmembranei485 – 50521HelicalSequence analysisAdd
    BLAST
    Transmembranei524 – 54421HelicalSequence analysisAdd
    BLAST
    Transmembranei568 – 58821HelicalSequence analysisAdd
    BLAST

    GO - Cellular componenti

    • integral component of membrane Source: UniProtKB-KW
    • plasma membrane Source: TAIR
    Complete GO annotation...

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype when grown under normal conditions. Growth retardation when starved of nitrogen.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 620620Protein NRT1/ PTR FAMILY 2.13PRO_0000399955Add
    BLAST

    Proteomic databases

    PaxDbiQ8RX77.
    PRIDEiQ8RX77.

    PTM databases

    iPTMnetiQ8RX77.

    Expressioni

    Tissue specificityi

    Expressed in leaves and flowers. Detected in stems and siliques. Highest expression in the distal lamina of older leaves. Restricted to the sieve element and companion cell complex of the minor vein.2 Publications

    Inductioni

    Circadian-regulation. Expression increase during the light phase and decrease during the dark phase.1 Publication

    Gene expression databases

    GenevisibleiQ8RX77. AT.

    Interactioni

    Protein-protein interaction databases

    BioGridi28544. 24 interactions.
    STRINGi3702.AT1G69870.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8RX77.
    SMRiQ8RX77. Positions 42-594.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG1237. Eukaryota.
    COG3104. LUCA.
    HOGENOMiHOG000237399.
    InParanoidiQ8RX77.
    OMAiPDERESN.
    OrthoDBiEOG093605YH.
    PhylomeDBiQ8RX77.

    Family and domain databases

    CDDicd06174. MFS. 1 hit.
    InterProiIPR020846. MFS_dom.
    IPR000109. POT_fam.
    [Graphical view]
    PANTHERiPTHR11654. PTHR11654. 1 hit.
    PfamiPF00854. PTR2. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Q8RX77-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MVLEDRKDGS SLPGRSGSFS KSSPSELDVV DPYKRISSPG SILDAEKVEK
    60 70 80 90 100
    KPGGWRAVSF ILGNETLERL GSIGLLANFM VYLTKVFHLE QVDAANVINI
    110 120 130 140 150
    WSGFTNLTPL VGAYISDTYV GRFKTIAFAS FATLLGLITI TLTASFPQLH
    160 170 180 190 200
    PASCNSQDPL SCGGPNKLQI GVLLLGLCFL SVGSGGIRPC SIPFGVDQFD
    210 220 230 240 250
    QRTEEGVKGV ASFFNWYYMT FTVVLIITQT VVVYIQDQVS WIIGFSIPTG
    260 270 280 290 300
    LMALAVVMFF AGMKRYVYVK PEGSIFSGIA QVIVAARKKR KLKLPAEDDG
    310 320 330 340 350
    TVTYYDPAIK SSVLSKLHRS NQFRCLDKAA VVIEGDLTPE GPPADKWRLC
    360 370 380 390 400
    SVQEVEEVKC LIRIVPIWSA GIISLAAMTT QGTFTVSQAL KMDRNLGPKF
    410 420 430 440 450
    EIPAGSLSVI SLLTIGIFLP FYDRVFVPFM RRITGHKSGI TLLQRIGTGI
    460 470 480 490 500
    VFAIFSMIVA GIVERMRRIR SINAGDPTGM TPMSVFWLSP QLILMGLCEA
    510 520 530 540 550
    FNIIGQIEFF NSQFPEHMRS IANSLFSLSF AGSSYLSSFL VTVVHKFSGG
    560 570 580 590 600
    HDRPDWLNKN LNAGKLDYFY YLIAVLGVVN LVYFWYCARG YRYKVGLPIE
    610 620
    DFEEDKSSDD VEMTSKKSMK
    Length:620
    Mass (Da):68,417
    Last modified:June 1, 2002 - v1
    Checksum:i3B554DEDC4C1E829
    GO

    Sequence cautioni

    The sequence AAG52567 differs from that shown. Reason: Erroneous gene model prediction. Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AC010675 Genomic DNA. Translation: AAG52567.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE34993.1.
    AY090257 mRNA. Translation: AAL90918.1.
    AY125531 mRNA. Translation: AAM78041.1.
    PIRiA96721.
    RefSeqiNP_564979.1. NM_105655.2.
    UniGeneiAt.23012.

    Genome annotation databases

    EnsemblPlantsiAT1G69870.1; AT1G69870.1; AT1G69870.
    GeneIDi843323.
    GrameneiAT1G69870.1; AT1G69870.1; AT1G69870.
    KEGGiath:AT1G69870.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AC010675 Genomic DNA. Translation: AAG52567.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE34993.1.
    AY090257 mRNA. Translation: AAL90918.1.
    AY125531 mRNA. Translation: AAM78041.1.
    PIRiA96721.
    RefSeqiNP_564979.1. NM_105655.2.
    UniGeneiAt.23012.

    3D structure databases

    ProteinModelPortaliQ8RX77.
    SMRiQ8RX77. Positions 42-594.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi28544. 24 interactions.
    STRINGi3702.AT1G69870.1.

    Protein family/group databases

    TCDBi2.A.17.3.15. the proton-dependent oligopeptide transporter (pot/ptr) family.

    PTM databases

    iPTMnetiQ8RX77.

    Proteomic databases

    PaxDbiQ8RX77.
    PRIDEiQ8RX77.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT1G69870.1; AT1G69870.1; AT1G69870.
    GeneIDi843323.
    GrameneiAT1G69870.1; AT1G69870.1; AT1G69870.
    KEGGiath:AT1G69870.

    Organism-specific databases

    TAIRiAT1G69870.

    Phylogenomic databases

    eggNOGiKOG1237. Eukaryota.
    COG3104. LUCA.
    HOGENOMiHOG000237399.
    InParanoidiQ8RX77.
    OMAiPDERESN.
    OrthoDBiEOG093605YH.
    PhylomeDBiQ8RX77.

    Enzyme and pathway databases

    ReactomeiR-ATH-427975. Proton/oligopeptide cotransporters.

    Miscellaneous databases

    PROiQ8RX77.

    Gene expression databases

    GenevisibleiQ8RX77. AT.

    Family and domain databases

    CDDicd06174. MFS. 1 hit.
    InterProiIPR020846. MFS_dom.
    IPR000109. POT_fam.
    [Graphical view]
    PANTHERiPTHR11654. PTHR11654. 1 hit.
    PfamiPF00854. PTR2. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 2 hits.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiPTR21_ARATH
    AccessioniPrimary (citable) accession number: Q8RX77
    Secondary accession number(s): Q9CAS0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 2, 2010
    Last sequence update: June 1, 2002
    Last modified: September 7, 2016
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.