Q8RX55 (B3GTI_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable beta-1,3-galactosyltransferase 18
EC=2.4.1.-

Gene names

Name:	B3GALT18
Ordered Locus Names:	At1g74800
ORF Names:	F25A4.23

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	672 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Beta-1,3-galactosyltransferase that transfers galactose from UDP-galactose to substrates with a terminal glycosyl residue By similarity.
Cofactor	Manganese By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Probable.
Tissue specificity	Expressed in juvenile leaves, stems, cauline leaves and siliques. Ref.4
Sequence similarities	Belongs to the glycosyltransferase 31 family. Contains 1 galectin domain.
Sequence caution	The sequence AAD55296.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Manganese
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	galactosyltransferase activity Inferred from electronic annotation. Source: InterPro sugar binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 672	672	Probable beta-1,3-galactosyltransferase 18		PRO_0000359428
Regions
	Transmembrane	29 – 49	21	Helical; Signal-anchor for type II membrane protein; Potential
	Domain	191 – 392	202	Galectin
Amino acid modifications
	Glycosylation	306	1	N-linked (GlcNAc...) Potential
	Glycosylation	620	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8RX55 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: A08ABB5E32233615	FASTA	672	77,348
	10 20 30 40 50 60 MKKPKLSKVE KIDKIDLFSS LWKQRSVRVI MAIGFLYLVI VSVEIPLVFK SWSSSSVPLD 70 80 90 100 110 120 ALSRLEKLNN EQEPQVEIIP NPPLEPVSYP VSNPTIVTRT DLVQNKVREH HRGVLSSLRF 130 140 150 160 170 180 DSETFDPSSK DGSVELHKSA KEAWQLGRKL WKELESGRLE KLVEKPEKNK PDSCPHSVSL 190 200 210 220 230 240 TGSEFMNREN KLMELPCGLT LGSHITLVGR PRKAHPKEGD WSKLVSQFVI ELQGLKTVEG 250 260 270 280 290 300 EDPPRILHFN PRLKGDWSKK PVIEQNSCYR MQWGPAQRCE GWKSRDDEET VDSHVKCEKW 310 320 330 340 350 360 IRDDDNYSEG SRARWWLNRL IGRRKRVKVE WPFPFVEEKL FVLTLSAGLE GYHINVDGKH 370 380 390 400 410 420 VTSFPYRTGF TLEDATGLTV NGDIDVHSVF VASLPTSHPS FAPQRHLELS KRWQAPVVPD 430 440 450 460 470 480 GPVEIFIGIL SAGNHFSERM AVRKSWMQHV LITSAKVVAR FFVALHGRKE VNVELKKEAE 490 500 510 520 530 540 YFGDIVLVPY MDSYDLVVLK TVAICEHGAL AFSAKYIMKC DDDTFVKLGA VINEVKKVPE 550 560 570 580 590 600 GRSLYIGNMN YYHKPLRGGK WAVTYEEWPE EDYPPYANGP GYVLSSDIAR FIVDKFERHK 610 620 630 640 650 660 LRLFKMEDVS VGMWVEHFKN TTNPVDYRHS LRFCQFGCVE NYYTAHYQSP RQMICLWDKL 670 LRQNKPECCN MR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"A unique beta-1,3-galactosyltransferase is indispensable for the biosynthesis of N-glycans containing Lewis a structures in Arabidopsis thaliana." Strasser R., Bondili J.S., Vavra U., Schoberer J., Svoboda B., Gloessl J., Leonard R., Stadlmann J., Altmann F., Steinkellner H., Mach L. Plant Cell 19:2278-2292(2007) [PubMed: 17630273] [Abstract] Cited for: TISSUE SPECIFICITY.
[5]	"Identification of a novel group of putative Arabidopsis thaliana beta-(1,3)-galactosyltransferases." Qu Y., Egelund J., Gilson P.R., Houghton F., Gleeson P.A., Schultz C.J., Bacic A. Plant Mol. Biol. 68:43-59(2008) [PubMed: 18548197] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC008263 Genomic DNA. Translation: AAD55296.1. Different initiation. CP002684 Genomic DNA. Translation: AEE35633.1. AY090451 mRNA. Translation: AAL91295.1.
IPI	IPI00538526.
PIR	D96777.
RefSeq	NP_177618.2. NM_106138.2.
UniGene	At.27816.
3D structure databases
ProteinModelPortal	Q8RX55.
SMR	Q8RX55. Positions 187-393.
ModBase	Search...
Protein family/group databases
CAZy	GT31. Glycosyltransferase Family 31.
Proteomic databases
PRIDE	Q8RX55.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G74800.1; AT1G74800.1; AT1G74800.
GeneID	843819.
GenomeReviews	Gene locus AT1G74800 in contig CT485782_GR.
KEGG	ath:AT1G74800.
Organism-specific databases
TAIR	At1g74800.
Phylogenomic databases
eggNOG	KOG2287.
GeneTree	EPGT00050000005432.
HOGENOM	HBG318248.
InParanoid	Q8RX55.
OMA	HGRKEVN.
PhylomeDB	Q8RX55.
ProtClustDB	CLSN2917913.
Gene expression databases
Genevestigator	Q8RX55.
Family and domain databases
InterPro	IPR008985. ConA-like_lec_gl. IPR013320. ConA-like_subgrp. IPR001079. Galectin_CRD. IPR002659. Glyco_trans_31. [Graphical view]
Gene3D	G3DSA:2.60.120.200. ConA_like_subgrp. 2 hits.
PANTHER	PTHR11214. Glyco_trans_31. 1 hit.
Pfam	PF00337. Gal-bind_lectin. 1 hit. PF01762. Galactosyl_T. 1 hit. [Graphical view]
SMART	SM00908. Gal-bind_lectin. 1 hit. SM00276. GLECT. 1 hit. [Graphical view]
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit.
PROSITE	PS51304. GALECTIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B3GTI_ARATH

Accession

Primary (citable) accession number: Q8RX55
Secondary accession number(s): Q9SSG4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	June 1, 2002
Last modified:	December 14, 2011
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program