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Protein

Hydroxyproline O-galactosyltransferase GALT5

Gene

GALT5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Possesses hydroxyproline O-galactosyltransferase activity. Transfers galactose from UDP-galactose to hydroxyproline residues in the arabinogalactan proteins (AGPs). Is specific for AGPs containing non-contiguous peptidyl hydroxyproline residues. Utilizes UDP-galactose solely as sugar donor. The addition of galactose onto the peptidyl hydroxyproline residues in AGP core proteins represents the first committed step in arabinogalactan polysaccharide addition. AGP glycans play essential roles in both vegetative and reproductive plant growth.2 Publications

Cofactori

Mn2+1 Publication

pH dependencei

Optimum pH is 6.5.1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.Curated
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • protein glycosylation Source: UniProtKB-UniPathway
  • root hair cell development Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT31. Glycosyltransferase Family 31.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyproline O-galactosyltransferase GALT51 Publication (EC:2.4.1.-1 Publication)
Short name:
AtGALT51 Publication
Alternative name(s):
Beta-1,3-galactosyltransferase 18Curated
Gene namesi
Name:GALT51 Publication
Synonyms:B3GALT181 Publication
Ordered Locus Names:At1g74800
ORF Names:F25A4.23
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G74800.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 28CytoplasmicCuratedAdd BLAST28
Transmembranei29 – 49Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini50 – 672LumenalCuratedAdd BLAST623

GO - Cellular componenti

  • endosome Source: TAIR
  • Golgi apparatus Source: TAIR
  • Golgi membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • trans-Golgi network Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

Reduced levels of arabinogalactan proteins (PubMed:26690932). Defects in root hair growth, root elongation, pollen tube growth, flowering time, leaf development, silique length and inflorescence growth. Increased sensitivity to salt stress (PubMed:25974423).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003594281 – 672Hydroxyproline O-galactosyltransferase GALT5Add BLAST672

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi306N-linked (GlcNAc...)Sequence analysis1
Glycosylationi620N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ8RX55.

Expressioni

Tissue specificityi

Expressed in juvenile leaves, stems, cauline leaves and siliques.1 Publication

Inductioni

By salt stress.1 Publication

Gene expression databases

ExpressionAtlasiQ8RX55. baseline and differential.
GenevisibleiQ8RX55. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G74800.1.

Structurei

3D structure databases

ProteinModelPortaliQ8RX55.
SMRiQ8RX55.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini191 – 392GalectinPROSITE-ProRule annotationAdd BLAST202

Sequence similaritiesi

Belongs to the glycosyltransferase 31 family.Curated
Contains 1 galectin domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2287. Eukaryota.
ENOG410ZZ1B. LUCA.
HOGENOMiHOG000239189.
InParanoidiQ8RX55.
OMAiVRVIMAI.
OrthoDBiEOG093603GO.
PhylomeDBiQ8RX55.

Family and domain databases

Gene3Di2.60.120.200. 2 hits.
InterProiIPR013320. ConA-like_dom.
IPR001079. Galectin_CRD.
IPR002659. Glyco_trans_31.
[Graphical view]
PANTHERiPTHR11214. PTHR11214. 1 hit.
PfamiPF00337. Gal-bind_lectin. 1 hit.
PF01762. Galactosyl_T. 1 hit.
[Graphical view]
SMARTiSM00908. Gal-bind_lectin. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 2 hits.
PROSITEiPS51304. GALECTIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8RX55-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKPKLSKVE KIDKIDLFSS LWKQRSVRVI MAIGFLYLVI VSVEIPLVFK
60 70 80 90 100
SWSSSSVPLD ALSRLEKLNN EQEPQVEIIP NPPLEPVSYP VSNPTIVTRT
110 120 130 140 150
DLVQNKVREH HRGVLSSLRF DSETFDPSSK DGSVELHKSA KEAWQLGRKL
160 170 180 190 200
WKELESGRLE KLVEKPEKNK PDSCPHSVSL TGSEFMNREN KLMELPCGLT
210 220 230 240 250
LGSHITLVGR PRKAHPKEGD WSKLVSQFVI ELQGLKTVEG EDPPRILHFN
260 270 280 290 300
PRLKGDWSKK PVIEQNSCYR MQWGPAQRCE GWKSRDDEET VDSHVKCEKW
310 320 330 340 350
IRDDDNYSEG SRARWWLNRL IGRRKRVKVE WPFPFVEEKL FVLTLSAGLE
360 370 380 390 400
GYHINVDGKH VTSFPYRTGF TLEDATGLTV NGDIDVHSVF VASLPTSHPS
410 420 430 440 450
FAPQRHLELS KRWQAPVVPD GPVEIFIGIL SAGNHFSERM AVRKSWMQHV
460 470 480 490 500
LITSAKVVAR FFVALHGRKE VNVELKKEAE YFGDIVLVPY MDSYDLVVLK
510 520 530 540 550
TVAICEHGAL AFSAKYIMKC DDDTFVKLGA VINEVKKVPE GRSLYIGNMN
560 570 580 590 600
YYHKPLRGGK WAVTYEEWPE EDYPPYANGP GYVLSSDIAR FIVDKFERHK
610 620 630 640 650
LRLFKMEDVS VGMWVEHFKN TTNPVDYRHS LRFCQFGCVE NYYTAHYQSP
660 670
RQMICLWDKL LRQNKPECCN MR
Length:672
Mass (Da):77,348
Last modified:June 1, 2002 - v1
Checksum:iA08ABB5E32233615
GO

Sequence cautioni

The sequence AAD55296 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
KJ138933 mRNA. Translation: AHL38873.1.
AC008263 Genomic DNA. Translation: AAD55296.1. Different initiation.
CP002684 Genomic DNA. Translation: AEE35633.1.
AY090451 mRNA. Translation: AAL91295.1.
PIRiD96777.
RefSeqiNP_177618.2. NM_106138.3.
UniGeneiAt.27816.

Genome annotation databases

EnsemblPlantsiAT1G74800.1; AT1G74800.1; AT1G74800.
GeneIDi843819.
GrameneiAT1G74800.1; AT1G74800.1; AT1G74800.
KEGGiath:AT1G74800.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
KJ138933 mRNA. Translation: AHL38873.1.
AC008263 Genomic DNA. Translation: AAD55296.1. Different initiation.
CP002684 Genomic DNA. Translation: AEE35633.1.
AY090451 mRNA. Translation: AAL91295.1.
PIRiD96777.
RefSeqiNP_177618.2. NM_106138.3.
UniGeneiAt.27816.

3D structure databases

ProteinModelPortaliQ8RX55.
SMRiQ8RX55.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G74800.1.

Protein family/group databases

CAZyiGT31. Glycosyltransferase Family 31.

Proteomic databases

PaxDbiQ8RX55.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G74800.1; AT1G74800.1; AT1G74800.
GeneIDi843819.
GrameneiAT1G74800.1; AT1G74800.1; AT1G74800.
KEGGiath:AT1G74800.

Organism-specific databases

TAIRiAT1G74800.

Phylogenomic databases

eggNOGiKOG2287. Eukaryota.
ENOG410ZZ1B. LUCA.
HOGENOMiHOG000239189.
InParanoidiQ8RX55.
OMAiVRVIMAI.
OrthoDBiEOG093603GO.
PhylomeDBiQ8RX55.

Enzyme and pathway databases

UniPathwayiUPA00378.

Miscellaneous databases

PROiQ8RX55.

Gene expression databases

ExpressionAtlasiQ8RX55. baseline and differential.
GenevisibleiQ8RX55. AT.

Family and domain databases

Gene3Di2.60.120.200. 2 hits.
InterProiIPR013320. ConA-like_dom.
IPR001079. Galectin_CRD.
IPR002659. Glyco_trans_31.
[Graphical view]
PANTHERiPTHR11214. PTHR11214. 1 hit.
PfamiPF00337. Gal-bind_lectin. 1 hit.
PF01762. Galactosyl_T. 1 hit.
[Graphical view]
SMARTiSM00908. Gal-bind_lectin. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 2 hits.
PROSITEiPS51304. GALECTIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiB3GTI_ARATH
AccessioniPrimary (citable) accession number: Q8RX55
Secondary accession number(s): Q9SSG4, W8QP19
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: June 1, 2002
Last modified: November 30, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.