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Protein

Amyrin synthase LUP2

Gene

LUP2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Multifunctional enzyme that converts oxidosqualene to nine different triterpenes, mainly lupeol, beta-amyrin and alpha-amyrin in a 15:50:30 ratio.2 Publications

Catalytic activityi

(3S)-2,3-epoxy-2,3-dihydrosqualene = beta-amyrin.1 Publication
(3S)-2,3-epoxy-2,3-dihydrosqualene = alpha-amyrin.1 Publication
(3S)-2,3-epoxy-2,3-dihydrosqualene = lupeol.1 Publication

GO - Molecular functioni

  • beta-amyrin synthase activity Source: TAIR

GO - Biological processi

  • triterpenoid biosynthetic process Source: TAIR

Keywordsi

Molecular functionIsomerase

Enzyme and pathway databases

ReactomeiR-ATH-191273 Cholesterol biosynthesis

Names & Taxonomyi

Protein namesi
Recommended name:
Amyrin synthase LUP2 (EC:5.4.99.39, EC:5.4.99.40, EC:5.4.99.41)
Alternative name(s):
Alpha-amyrin synthase
Beta-amyrin synthase
Lupeol synthase 2
Short name:
AtLUP2
Gene namesi
Name:LUP2
Ordered Locus Names:At1g78960
ORF Names:YUP8H12R.43
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G78960
TAIRilocus:2207300 AT1G78960

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003661321 – 763Amyrin synthase LUP2Add BLAST763

Proteomic databases

PaxDbiQ8RWT0

Expressioni

Gene expression databases

ExpressionAtlasiQ8RWT0 baseline and differential
GenevisibleiQ8RWT0 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G78960.1

Structurei

3D structure databases

ProteinModelPortaliQ8RWT0
SMRiQ8RWT0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati149 – 190PFTB 1Add BLAST42
Repeati592 – 632PFTB 2Add BLAST41
Repeati641 – 682PFTB 3Add BLAST42

Sequence similaritiesi

Belongs to the terpene cyclase/mutase family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0497 Eukaryota
COG1657 LUCA
HOGENOMiHOG000234317
InParanoidiQ8RWT0
KOiK15815
OMAiCAKEDMI
OrthoDBiEOG0936031Q
PhylomeDBiQ8RWT0

Family and domain databases

CDDicd02892 SQCY_1, 1 hit
InterProiView protein in InterPro
IPR032696 SQ_cyclase_C
IPR032697 SQ_cyclase_N
IPR018333 Squalene_cyclase
IPR002365 Terpene_synthase_CS
IPR008930 Terpenoid_cyclase/PrenylTrfase
PfamiView protein in Pfam
PF13243 SQHop_cyclase_C, 1 hit
PF13249 SQHop_cyclase_N, 1 hit
SUPFAMiSSF48239 SSF48239, 2 hits
TIGRFAMsiTIGR01787 squalene_cyclas, 1 hit
PROSITEiView protein in PROSITE
PS01074 TERPENE_SYNTHASES, 1 hit

Sequencei

Sequence statusi: Complete.

Q8RWT0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWKLKIGEGN GEDPYLFSSN NFVGRQTWEF DPKAGTPEER AAVEDARRNY
60 70 80 90 100
LDNRPRVKGC SDLLWRMQFL KEAKFEQVIP PVKIDDGEGI TYKNATDALR
110 120 130 140 150
RAVSFYSALQ SSDGHWPAEI TGTLFFLPPL VFCFYITGHL EKIFDAEHRK
160 170 180 190 200
EMLRHIYCHQ NEDGGWGLHI EGKSVMFCTV LNYICLRMLG EGPNGGRNNA
210 220 230 240 250
CKRARQWILD HGGVTYIPSW GKIWLSILGI YDWSGTNPMP PEIWLLPSFF
260 270 280 290 300
PIHLGKTLCY TRMVYMPMSY LYGKRFVGPL TPLIMLLRKE LHLQPYEEIN
310 320 330 340 350
WNKARRLCAK EDMIYPHPLV QDLLWDTLHN FVEPILTNWP LKKLVREKAL
360 370 380 390 400
RVAMEHIHYE DENSHYITIG CVEKVLCMLA CWIENPNGDH FKKHLARIPD
410 420 430 440 450
FMWVAEDGLK MQSFGSQLWD TVFAIQALLA CDLSDETDDV LRKGHSFIKK
460 470 480 490 500
SQVRENPSGD FKSMYRHISK GAWTLSDRDH GWQVSDCTAE ALKCCMLLSM
510 520 530 540 550
MPAEVVGQKI DPEQLYDSVN LLLSLQGEKG GLTAWEPVRA QEWLELLNPT
560 570 580 590 600
DFFTCVMAER EYVECTSAVI QALVLFKQLY PDHRTKEIIK SIEKGVQFIE
610 620 630 640 650
SKQTPDGSWH GNWGICFIYA TWFALSGLAA AGKTYKSCLA VRKGVDFLLA
660 670 680 690 700
IQEEDGGWGE SHLSCPEQRY IPLEGNRSNL VQTAWAMMGL IHAGQAERDP
710 720 730 740 750
TPLHRAAKLI ITSQLENGDF PQQEILGVFM NTCMLHYATY RNIFPLWALA
760
EYRKAAFATH QDL
Length:763
Mass (Da):87,515
Last modified:June 1, 2002 - v1
Checksum:i1D0147DD380FD698
GO

Sequence cautioni

The sequence AAC17070 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence BAD94022 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti515L → S in AAC98864 (PubMed:11247608).Curated1
Sequence conflicti540A → P in AAC98864 (PubMed:11247608).Curated1
Sequence conflicti578Q → R in AAC98864 (PubMed:11247608).Curated1
Sequence conflicti667E → G in AAC98864 (PubMed:11247608).Curated1
Sequence conflicti676N → D in AAC98864 (PubMed:11247608).Curated1
Sequence conflicti686A → G in AAC98864 (PubMed:11247608).Curated1
Sequence conflicti710 – 712IIT → VIS in AAC98864 (PubMed:11247608).Curated3
Sequence conflicti722Q → R in AAC98864 (PubMed:11247608).Curated1
Sequence conflicti731N → K in AAC98864 (PubMed:11247608).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002986 Genomic DNA Translation: AAC17070.1 Sequence problems.
CP002684 Genomic DNA Translation: AEE36185.1
CP002684 Genomic DNA Translation: ANM60689.1
CP002684 Genomic DNA Translation: ANM60690.1
AY091131 mRNA Translation: AAM14080.1
AY114039 mRNA Translation: AAM45087.1
AF003472 mRNA Translation: AAC98864.1
AK230119 mRNA Translation: BAF01935.1
AK221820 mRNA Translation: BAD94022.1 Different initiation.
PIRiT01059
RefSeqiNP_001322959.1, NM_001334861.1
NP_001322960.1, NM_001334862.1
NP_178017.2, NM_106545.4
UniGeneiAt.34204

Genome annotation databases

EnsemblPlantsiAT1G78960.1; AT1G78960.1; AT1G78960
AT1G78960.2; AT1G78960.2; AT1G78960
AT1G78960.3; AT1G78960.3; AT1G78960
GeneIDi844236
GrameneiAT1G78960.1; AT1G78960.1; AT1G78960
AT1G78960.2; AT1G78960.2; AT1G78960
AT1G78960.3; AT1G78960.3; AT1G78960
KEGGiath:AT1G78960

Similar proteinsi

Entry informationi

Entry nameiLUP2_ARATH
AccessioniPrimary (citable) accession number: Q8RWT0
Secondary accession number(s): O64552
, Q0WLS2, Q56X57, Q84L74
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 3, 2009
Last sequence update: June 1, 2002
Last modified: May 23, 2018
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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