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Protein

Amyrin synthase LUP2

Gene

LUP2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Multifunctional enzyme that converts oxidosqualene to nine different triterpenes, mainly lupeol, beta-amyrin and alpha-amyrin in a 15:50:30 ratio.2 Publications

Catalytic activityi

(3S)-2,3-epoxy-2,3-dihydrosqualene = beta-amyrin.1 Publication
(3S)-2,3-epoxy-2,3-dihydrosqualene = alpha-amyrin.1 Publication
(3S)-2,3-epoxy-2,3-dihydrosqualene = lupeol.1 Publication

GO - Molecular functioni

  • beta-amyrin synthase activity Source: TAIR

GO - Biological processi

  • triterpenoid biosynthetic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Names & Taxonomyi

Protein namesi
Recommended name:
Amyrin synthase LUP2 (EC:5.4.99.39, EC:5.4.99.40, EC:5.4.99.41)
Alternative name(s):
Alpha-amyrin synthase
Beta-amyrin synthase
Lupeol synthase 2
Short name:
AtLUP2
Gene namesi
Name:LUP2
Ordered Locus Names:At1g78960
ORF Names:YUP8H12R.43
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G78960.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 763763Amyrin synthase LUP2PRO_0000366132Add
BLAST

Proteomic databases

PaxDbiQ8RWT0.
PRIDEiQ8RWT0.

Expressioni

Gene expression databases

GenevisibleiQ8RWT0. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G78960.1.

Structurei

3D structure databases

ProteinModelPortaliQ8RWT0.
SMRiQ8RWT0. Positions 99-280.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati149 – 19042PFTB 1Add
BLAST
Repeati592 – 63241PFTB 2Add
BLAST
Repeati641 – 68242PFTB 3Add
BLAST

Sequence similaritiesi

Belongs to the terpene cyclase/mutase family.Curated
Contains 3 PFTB repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0497. Eukaryota.
COG1657. LUCA.
HOGENOMiHOG000234317.
InParanoidiQ8RWT0.
KOiK15815.
OMAiCAKEDMI.
OrthoDBiEOG0936031Q.
PhylomeDBiQ8RWT0.

Family and domain databases

Gene3Di1.50.10.20. 2 hits.
InterProiIPR032696. SQ_cyclase_C.
IPR032697. SQ_cyclase_N.
IPR018333. Squalene_cyclase.
IPR002365. Terpene_synthase_CS.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF13243. SQHop_cyclase_C. 1 hit.
PF13249. SQHop_cyclase_N. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 2 hits.
TIGRFAMsiTIGR01787. squalene_cyclas. 1 hit.
PROSITEiPS01074. TERPENE_SYNTHASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8RWT0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWKLKIGEGN GEDPYLFSSN NFVGRQTWEF DPKAGTPEER AAVEDARRNY
60 70 80 90 100
LDNRPRVKGC SDLLWRMQFL KEAKFEQVIP PVKIDDGEGI TYKNATDALR
110 120 130 140 150
RAVSFYSALQ SSDGHWPAEI TGTLFFLPPL VFCFYITGHL EKIFDAEHRK
160 170 180 190 200
EMLRHIYCHQ NEDGGWGLHI EGKSVMFCTV LNYICLRMLG EGPNGGRNNA
210 220 230 240 250
CKRARQWILD HGGVTYIPSW GKIWLSILGI YDWSGTNPMP PEIWLLPSFF
260 270 280 290 300
PIHLGKTLCY TRMVYMPMSY LYGKRFVGPL TPLIMLLRKE LHLQPYEEIN
310 320 330 340 350
WNKARRLCAK EDMIYPHPLV QDLLWDTLHN FVEPILTNWP LKKLVREKAL
360 370 380 390 400
RVAMEHIHYE DENSHYITIG CVEKVLCMLA CWIENPNGDH FKKHLARIPD
410 420 430 440 450
FMWVAEDGLK MQSFGSQLWD TVFAIQALLA CDLSDETDDV LRKGHSFIKK
460 470 480 490 500
SQVRENPSGD FKSMYRHISK GAWTLSDRDH GWQVSDCTAE ALKCCMLLSM
510 520 530 540 550
MPAEVVGQKI DPEQLYDSVN LLLSLQGEKG GLTAWEPVRA QEWLELLNPT
560 570 580 590 600
DFFTCVMAER EYVECTSAVI QALVLFKQLY PDHRTKEIIK SIEKGVQFIE
610 620 630 640 650
SKQTPDGSWH GNWGICFIYA TWFALSGLAA AGKTYKSCLA VRKGVDFLLA
660 670 680 690 700
IQEEDGGWGE SHLSCPEQRY IPLEGNRSNL VQTAWAMMGL IHAGQAERDP
710 720 730 740 750
TPLHRAAKLI ITSQLENGDF PQQEILGVFM NTCMLHYATY RNIFPLWALA
760
EYRKAAFATH QDL
Length:763
Mass (Da):87,515
Last modified:June 1, 2002 - v1
Checksum:i1D0147DD380FD698
GO

Sequence cautioni

The sequence AAC17070 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence BAD94022 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti515 – 5151L → S in AAC98864 (PubMed:11247608).Curated
Sequence conflicti540 – 5401A → P in AAC98864 (PubMed:11247608).Curated
Sequence conflicti578 – 5781Q → R in AAC98864 (PubMed:11247608).Curated
Sequence conflicti667 – 6671E → G in AAC98864 (PubMed:11247608).Curated
Sequence conflicti676 – 6761N → D in AAC98864 (PubMed:11247608).Curated
Sequence conflicti686 – 6861A → G in AAC98864 (PubMed:11247608).Curated
Sequence conflicti710 – 7123IIT → VIS in AAC98864 (PubMed:11247608).Curated
Sequence conflicti722 – 7221Q → R in AAC98864 (PubMed:11247608).Curated
Sequence conflicti731 – 7311N → K in AAC98864 (PubMed:11247608).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002986 Genomic DNA. Translation: AAC17070.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE36185.1.
AY091131 mRNA. Translation: AAM14080.1.
AY114039 mRNA. Translation: AAM45087.1.
AF003472 mRNA. Translation: AAC98864.1.
AK230119 mRNA. Translation: BAF01935.1.
AK221820 mRNA. Translation: BAD94022.1. Different initiation.
PIRiT01059.
RefSeqiNP_178017.2. NM_106545.3.
UniGeneiAt.34204.

Genome annotation databases

EnsemblPlantsiAT1G78960.1; AT1G78960.1; AT1G78960.
GeneIDi844236.
GrameneiAT1G78960.1; AT1G78960.1; AT1G78960.
KEGGiath:AT1G78960.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002986 Genomic DNA. Translation: AAC17070.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE36185.1.
AY091131 mRNA. Translation: AAM14080.1.
AY114039 mRNA. Translation: AAM45087.1.
AF003472 mRNA. Translation: AAC98864.1.
AK230119 mRNA. Translation: BAF01935.1.
AK221820 mRNA. Translation: BAD94022.1. Different initiation.
PIRiT01059.
RefSeqiNP_178017.2. NM_106545.3.
UniGeneiAt.34204.

3D structure databases

ProteinModelPortaliQ8RWT0.
SMRiQ8RWT0. Positions 99-280.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G78960.1.

Proteomic databases

PaxDbiQ8RWT0.
PRIDEiQ8RWT0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G78960.1; AT1G78960.1; AT1G78960.
GeneIDi844236.
GrameneiAT1G78960.1; AT1G78960.1; AT1G78960.
KEGGiath:AT1G78960.

Organism-specific databases

TAIRiAT1G78960.

Phylogenomic databases

eggNOGiKOG0497. Eukaryota.
COG1657. LUCA.
HOGENOMiHOG000234317.
InParanoidiQ8RWT0.
KOiK15815.
OMAiCAKEDMI.
OrthoDBiEOG0936031Q.
PhylomeDBiQ8RWT0.

Miscellaneous databases

PROiQ8RWT0.

Gene expression databases

GenevisibleiQ8RWT0. AT.

Family and domain databases

Gene3Di1.50.10.20. 2 hits.
InterProiIPR032696. SQ_cyclase_C.
IPR032697. SQ_cyclase_N.
IPR018333. Squalene_cyclase.
IPR002365. Terpene_synthase_CS.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF13243. SQHop_cyclase_C. 1 hit.
PF13249. SQHop_cyclase_N. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 2 hits.
TIGRFAMsiTIGR01787. squalene_cyclas. 1 hit.
PROSITEiPS01074. TERPENE_SYNTHASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLUP2_ARATH
AccessioniPrimary (citable) accession number: Q8RWT0
Secondary accession number(s): O64552
, Q0WLS2, Q56X57, Q84L74
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 3, 2009
Last sequence update: June 1, 2002
Last modified: September 7, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.