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Q8RWN7

- P2C32_ARATH

UniProt

Q8RWN7 - P2C32_ARATH

Protein

Protein phosphatase 2C 32

Gene

POL

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 2 (03 May 2011)
      Previous versions | rss
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    Functioni

    Involved in the regulation of pedicel length and of CLAVATA pathways controlling stem cell identity at shoot and flower meristems.4 Publications

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 magnesium or manganese ions per subunit.By similarity

    Enzyme regulationi

    Insensitive to okadaic acid.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi307 – 3071Manganese 1By similarity
    Metal bindingi307 – 3071Manganese 2By similarity
    Metal bindingi308 – 3081Manganese 1; via carbonyl oxygenBy similarity
    Metal bindingi763 – 7631Manganese 2By similarity
    Metal bindingi826 – 8261Manganese 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. phospholipid binding Source: TAIR
    3. protein serine/threonine phosphatase activity Source: TAIR

    GO - Biological processi

    1. dephosphorylation Source: GOC
    2. maintenance of meristem identity Source: TAIR
    3. regulation of meristem structural organization Source: TAIR
    4. regulation of transcription, DNA-templated Source: TAIR

    Keywords - Molecular functioni

    Developmental protein, Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciARA:AT2G46920-MONOMER.
    ARA:GQT-475-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein phosphatase 2C 32 (EC:3.1.3.16)
    Short name:
    AtPP2C32
    Alternative name(s):
    Protein POLTERGEIST
    Protein phosphatase 2C POL
    Short name:
    PP2C POL
    Gene namesi
    Name:POL
    Ordered Locus Names:At2g46920
    ORF Names:F14M4.25
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G46920.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell
    2. plasma membrane Source: TAIR

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Loss-of-function mutant pol-6 (T-DNA insertion) shows suppression of clavata mutant phenotypes. The catalytic activity of POL may be functionally replaced by the activity of PLL1. Pol and pll1 double mutant is seedling lethal.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi287 – 2871E → K in pol-1; reduced catalytic activity and partial phenotypic suppression of mutations within CLAVATA. 1 Publication
    Mutagenesisi443 – 856414Missing in pol-3; weak phenotypic suppression of mutations within CLAVATA. Add
    BLAST

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 856856Protein phosphatase 2C 32PRO_0000301258Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei152 – 1521Phosphoserine2 Publications
    Modified residuei189 – 1891Phosphoserine2 Publications
    Modified residuei201 – 2011PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ8RWN7.
    PRIDEiQ8RWN7.

    Expressioni

    Tissue specificityi

    Expressed in roots, leaves, stems, inflorescences, flowers and throughout the shoot meristem.2 Publications

    Developmental stagei

    Expressed throughout development of the floral meristem, in all four floral whorls and as floral organs matured, limited to the ovule.1 Publication

    Gene expression databases

    GenevestigatoriQ8RWN7.

    Interactioni

    Protein-protein interaction databases

    IntActiQ8RWN7. 1 interaction.
    MINTiMINT-8390824.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8RWN7.
    SMRiQ8RWN7. Positions 667-839.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini433 – 807375PP2C-likeAdd
    BLAST

    Domaini

    The N-terminal domain (1-233) has a regulatory function and inhibits phosphatase activity.
    The conserved PP2C phosphatase domain (271-833) is interrupted by an insertion of approximately 200 amino acids.

    Sequence similaritiesi

    Belongs to the PP2C family.Curated
    Contains 1 PP2C-like domain.Curated

    Phylogenomic databases

    eggNOGiNOG267184.
    HOGENOMiHOG000239375.
    InParanoidiQ8RWN7.
    OMAiKGDLRHR.

    Family and domain databases

    Gene3Di3.60.40.10. 3 hits.
    InterProiIPR001932. PP2C-like_dom.
    IPR015655. Protein_Pase_2C.
    [Graphical view]
    PANTHERiPTHR13832. PTHR13832. 1 hit.
    PfamiPF00481. PP2C. 2 hits.
    [Graphical view]
    SMARTiSM00332. PP2Cc. 1 hit.
    [Graphical view]
    SUPFAMiSSF81606. SSF81606. 3 hits.

    Sequencei

    Sequence statusi: Complete.

    Q8RWN7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGNGTSRVVG CFVPSNDKNG VDLEFLEPLD EGLGHSFCYV RPSIFESPDI    50
    TPSNSERFTI DSSTIDSETL TGSFRNDIVD DPSFLNRHNS KGLAETTFKA 100
    ISGASVSANV STARTGNQMA LCSSDVLEPA ASFESTSSFA SIPLQPLPRG 150
    GSGPLNGFMS GPLERGFASG PLDRNNGFMS GPIEKGVMSG PLDVSDRSNF 200
    SAPLSFRRKK PRFQRFMRSV SGPMKSTLAR TFSRRSGGLS WMHRFFLHPE 250
    TRVSWAVGKD GKLHGEDPES CLESNRNLQW AHGKAGEDRV HVVLSEEQGW 300
    LFIGIYDGFS GPDAPDFVMS HLYKAIDKEL EGLLWDYEEP SEDNQLQPDQ 350
    EPPTEENMCD PESISEQHSK SVVAESEEVM IDDISSLGNT DTQIADGPPG 400
    DSAGPGKKSM RLYELLQLEQ WEGEEIGLKR YGGNVALNNM TNQVENPSTS 450
    GGGAGNDPCT TDRSALDGIP NSGQRHGTKK SQISSKIRRM YQKQKSLRKK 500
    LFPWSYDWHR EEGICVEEKI VESSGPIRRR WSGTVDHDAV LRAMARALES 550
    TEEAYMDMVE KSLDINPELA LMGSCVLVML MKDQDVYVMN VGDSRAILAQ 600
    ERLHDRHSNP GFGNDEGIGH KSRSRESLVR IELDRISEES PIHNQATPIS 650
    VSNKNRDVTS YRLKMRAVQL SSDHSTSVEE EIWRIRSEHP EDDQSILKDR 700
    VKGQLKVTRA FGAGFLKKPN FNEALLEMFQ VEYIGTDPYI TCEPCTVHHR 750
    LTSSDRFMVL SSDGLYEYFS NEEVVAHVTW FIENVPEGDP AQYLIAELLS 800
    RAATKNGMEF HDLLDIPQGD RRKYHDDVSV MVVSLEGRIW RSSGQYYPER 850
    KQKFNR 856
    Length:856
    Mass (Da):95,571
    Last modified:May 3, 2011 - v2
    Checksum:i95BDFC8DEB5DE4F2
    GO

    Sequence cautioni

    The sequence AAC34239.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti769 – 7691F → Y in AAM12971. (PubMed:14593172)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC004411 Genomic DNA. Translation: AAC34239.1. Sequence problems.
    CP002685 Genomic DNA. Translation: AEC10772.1.
    CP002685 Genomic DNA. Translation: AEC10773.1.
    AY092972 mRNA. Translation: AAM12971.1.
    PIRiT02195.
    RefSeqiNP_850463.1. NM_180132.2.
    NP_850464.1. NM_180133.1.
    UniGeneiAt.25596.

    Genome annotation databases

    EnsemblPlantsiAT2G46920.1; AT2G46920.1; AT2G46920.
    AT2G46920.2; AT2G46920.2; AT2G46920.
    GeneIDi819306.
    KEGGiath:AT2G46920.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC004411 Genomic DNA. Translation: AAC34239.1 . Sequence problems.
    CP002685 Genomic DNA. Translation: AEC10772.1 .
    CP002685 Genomic DNA. Translation: AEC10773.1 .
    AY092972 mRNA. Translation: AAM12971.1 .
    PIRi T02195.
    RefSeqi NP_850463.1. NM_180132.2.
    NP_850464.1. NM_180133.1.
    UniGenei At.25596.

    3D structure databases

    ProteinModelPortali Q8RWN7.
    SMRi Q8RWN7. Positions 667-839.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q8RWN7. 1 interaction.
    MINTi MINT-8390824.

    Proteomic databases

    PaxDbi Q8RWN7.
    PRIDEi Q8RWN7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G46920.1 ; AT2G46920.1 ; AT2G46920 .
    AT2G46920.2 ; AT2G46920.2 ; AT2G46920 .
    GeneIDi 819306.
    KEGGi ath:AT2G46920.

    Organism-specific databases

    TAIRi AT2G46920.

    Phylogenomic databases

    eggNOGi NOG267184.
    HOGENOMi HOG000239375.
    InParanoidi Q8RWN7.
    OMAi KGDLRHR.

    Enzyme and pathway databases

    BioCyci ARA:AT2G46920-MONOMER.
    ARA:GQT-475-MONOMER.

    Gene expression databases

    Genevestigatori Q8RWN7.

    Family and domain databases

    Gene3Di 3.60.40.10. 3 hits.
    InterProi IPR001932. PP2C-like_dom.
    IPR015655. Protein_Pase_2C.
    [Graphical view ]
    PANTHERi PTHR13832. PTHR13832. 1 hit.
    Pfami PF00481. PP2C. 2 hits.
    [Graphical view ]
    SMARTi SM00332. PP2Cc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF81606. SSF81606. 3 hits.
    ProtoNeti Search...

    Publicationsi

    1. "POLTERGEIST encodes a protein phosphatase 2C that regulates CLAVATA pathways controlling stem cell identity at Arabidopsis shoot and flower meristems."
      Yu L.P., Miller A.K., Clark S.E.
      Curr. Biol. 13:179-188(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF GLU-287 AND 443-GLN--ARG-856, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, FUNCTION, ENZYME REGULATION.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "POLTERGEIST functions to regulate meristem development downstream of the CLAVATA loci."
      Yu L.P., Simon E.J., Trotochaud A.E., Clark S.E.
      Development 127:1661-1670(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Large-scale analysis of in vivo phosphorylated membrane proteins by immobilized metal ion affinity chromatography and mass spectrometry."
      Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.
      Mol. Cell. Proteomics 2:1234-1243(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-152 AND SER-189, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: cv. La-0.
    7. "Phosphoproteomics of the Arabidopsis plasma membrane and a new phosphorylation site database."
      Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.
      Plant Cell 16:2394-2405(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-152 AND SER-189, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "POL and related phosphatases are dosage-sensitive regulators of meristem and organ development in Arabidopsis."
      Song S.-K., Clark S.E.
      Dev. Biol. 285:272-284(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE, DISRUPTION PHENOTYPE.
    9. "POL and PLL1 phosphatases are CLAVATA1 signaling intermediates required for Arabidopsis shoot and floral stem cells."
      Song S.-K., Lee M.M., Clark S.E.
      Development 133:4691-4698(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    10. "Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
      Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
      BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiP2C32_ARATH
    AccessioniPrimary (citable) accession number: Q8RWN7
    Secondary accession number(s): O80732
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 11, 2007
    Last sequence update: May 3, 2011
    Last modified: October 1, 2014
    This is version 77 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3