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Q8RWN7

- P2C32_ARATH

UniProt

Q8RWN7 - P2C32_ARATH

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Protein

Protein phosphatase 2C 32

Gene

POL

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in the regulation of pedicel length and of CLAVATA pathways controlling stem cell identity at shoot and flower meristems.4 Publications

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 2 magnesium or manganese ions per subunit.By similarity

Enzyme regulationi

Insensitive to okadaic acid.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi307 – 3071Manganese 1By similarity
Metal bindingi307 – 3071Manganese 2By similarity
Metal bindingi308 – 3081Manganese 1; via carbonyl oxygenBy similarity
Metal bindingi763 – 7631Manganese 2By similarity
Metal bindingi826 – 8261Manganese 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. phospholipid binding Source: TAIR
  3. protein serine/threonine phosphatase activity Source: TAIR

GO - Biological processi

  1. dephosphorylation Source: GOC
  2. maintenance of meristem identity Source: TAIR
  3. regulation of meristem structural organization Source: TAIR
  4. regulation of transcription, DNA-templated Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Hydrolase, Protein phosphatase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT2G46920-MONOMER.
ARA:GQT-475-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 2C 32 (EC:3.1.3.16)
Short name:
AtPP2C32
Alternative name(s):
Protein POLTERGEIST
Protein phosphatase 2C POL
Short name:
PP2C POL
Gene namesi
Name:POL
Ordered Locus Names:At2g46920
ORF Names:F14M4.25
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G46920.

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. nucleus Source: UniProtKB-KW
  2. plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Loss-of-function mutant pol-6 (T-DNA insertion) shows suppression of clavata mutant phenotypes. The catalytic activity of POL may be functionally replaced by the activity of PLL1. Pol and pll1 double mutant is seedling lethal.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi287 – 2871E → K in pol-1; reduced catalytic activity and partial phenotypic suppression of mutations within CLAVATA. 1 Publication
Mutagenesisi443 – 856414Missing in pol-3; weak phenotypic suppression of mutations within CLAVATA. 1 PublicationAdd
BLAST

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 856856Protein phosphatase 2C 32PRO_0000301258Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei152 – 1521Phosphoserine2 Publications
Modified residuei189 – 1891Phosphoserine2 Publications
Modified residuei201 – 2011PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8RWN7.
PRIDEiQ8RWN7.

Expressioni

Tissue specificityi

Expressed in roots, leaves, stems, inflorescences, flowers and throughout the shoot meristem.2 Publications

Developmental stagei

Expressed throughout development of the floral meristem, in all four floral whorls and as floral organs matured, limited to the ovule.1 Publication

Gene expression databases

ExpressionAtlasiQ8RWN7. baseline and differential.
GenevestigatoriQ8RWN7.

Interactioni

Protein-protein interaction databases

IntActiQ8RWN7. 1 interaction.
MINTiMINT-8390824.

Structurei

3D structure databases

ProteinModelPortaliQ8RWN7.
SMRiQ8RWN7. Positions 667-839.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini433 – 807375PP2C-likeAdd
BLAST

Domaini

The N-terminal domain (1-233) has a regulatory function and inhibits phosphatase activity.
The conserved PP2C phosphatase domain (271-833) is interrupted by an insertion of approximately 200 amino acids.

Sequence similaritiesi

Belongs to the PP2C family.Curated
Contains 1 PP2C-like domain.Curated

Phylogenomic databases

eggNOGiNOG267184.
HOGENOMiHOG000239375.
InParanoidiQ8RWN7.
OMAiKGDLRHR.

Family and domain databases

Gene3Di3.60.40.10. 3 hits.
InterProiIPR001932. PP2C-like_dom.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 2 hits.
[Graphical view]
SMARTiSM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 3 hits.

Sequencei

Sequence statusi: Complete.

Q8RWN7 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGNGTSRVVG CFVPSNDKNG VDLEFLEPLD EGLGHSFCYV RPSIFESPDI
60 70 80 90 100
TPSNSERFTI DSSTIDSETL TGSFRNDIVD DPSFLNRHNS KGLAETTFKA
110 120 130 140 150
ISGASVSANV STARTGNQMA LCSSDVLEPA ASFESTSSFA SIPLQPLPRG
160 170 180 190 200
GSGPLNGFMS GPLERGFASG PLDRNNGFMS GPIEKGVMSG PLDVSDRSNF
210 220 230 240 250
SAPLSFRRKK PRFQRFMRSV SGPMKSTLAR TFSRRSGGLS WMHRFFLHPE
260 270 280 290 300
TRVSWAVGKD GKLHGEDPES CLESNRNLQW AHGKAGEDRV HVVLSEEQGW
310 320 330 340 350
LFIGIYDGFS GPDAPDFVMS HLYKAIDKEL EGLLWDYEEP SEDNQLQPDQ
360 370 380 390 400
EPPTEENMCD PESISEQHSK SVVAESEEVM IDDISSLGNT DTQIADGPPG
410 420 430 440 450
DSAGPGKKSM RLYELLQLEQ WEGEEIGLKR YGGNVALNNM TNQVENPSTS
460 470 480 490 500
GGGAGNDPCT TDRSALDGIP NSGQRHGTKK SQISSKIRRM YQKQKSLRKK
510 520 530 540 550
LFPWSYDWHR EEGICVEEKI VESSGPIRRR WSGTVDHDAV LRAMARALES
560 570 580 590 600
TEEAYMDMVE KSLDINPELA LMGSCVLVML MKDQDVYVMN VGDSRAILAQ
610 620 630 640 650
ERLHDRHSNP GFGNDEGIGH KSRSRESLVR IELDRISEES PIHNQATPIS
660 670 680 690 700
VSNKNRDVTS YRLKMRAVQL SSDHSTSVEE EIWRIRSEHP EDDQSILKDR
710 720 730 740 750
VKGQLKVTRA FGAGFLKKPN FNEALLEMFQ VEYIGTDPYI TCEPCTVHHR
760 770 780 790 800
LTSSDRFMVL SSDGLYEYFS NEEVVAHVTW FIENVPEGDP AQYLIAELLS
810 820 830 840 850
RAATKNGMEF HDLLDIPQGD RRKYHDDVSV MVVSLEGRIW RSSGQYYPER

KQKFNR
Length:856
Mass (Da):95,571
Last modified:May 3, 2011 - v2
Checksum:i95BDFC8DEB5DE4F2
GO

Sequence cautioni

The sequence AAC34239.1 differs from that shown. Reason: Erroneous gene model prediction.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti769 – 7691F → Y in AAM12971. (PubMed:14593172)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC004411 Genomic DNA. Translation: AAC34239.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC10772.1.
CP002685 Genomic DNA. Translation: AEC10773.1.
AY092972 mRNA. Translation: AAM12971.1.
PIRiT02195.
RefSeqiNP_850463.1. NM_180132.2.
NP_850464.1. NM_180133.1.
UniGeneiAt.25596.

Genome annotation databases

EnsemblPlantsiAT2G46920.1; AT2G46920.1; AT2G46920.
AT2G46920.2; AT2G46920.2; AT2G46920.
GeneIDi819306.
KEGGiath:AT2G46920.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC004411 Genomic DNA. Translation: AAC34239.1 . Sequence problems.
CP002685 Genomic DNA. Translation: AEC10772.1 .
CP002685 Genomic DNA. Translation: AEC10773.1 .
AY092972 mRNA. Translation: AAM12971.1 .
PIRi T02195.
RefSeqi NP_850463.1. NM_180132.2.
NP_850464.1. NM_180133.1.
UniGenei At.25596.

3D structure databases

ProteinModelPortali Q8RWN7.
SMRi Q8RWN7. Positions 667-839.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q8RWN7. 1 interaction.
MINTi MINT-8390824.

Proteomic databases

PaxDbi Q8RWN7.
PRIDEi Q8RWN7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G46920.1 ; AT2G46920.1 ; AT2G46920 .
AT2G46920.2 ; AT2G46920.2 ; AT2G46920 .
GeneIDi 819306.
KEGGi ath:AT2G46920.

Organism-specific databases

TAIRi AT2G46920.

Phylogenomic databases

eggNOGi NOG267184.
HOGENOMi HOG000239375.
InParanoidi Q8RWN7.
OMAi KGDLRHR.

Enzyme and pathway databases

BioCyci ARA:AT2G46920-MONOMER.
ARA:GQT-475-MONOMER.

Gene expression databases

ExpressionAtlasi Q8RWN7. baseline and differential.
Genevestigatori Q8RWN7.

Family and domain databases

Gene3Di 3.60.40.10. 3 hits.
InterProi IPR001932. PP2C-like_dom.
IPR015655. Protein_Pase_2C.
[Graphical view ]
PANTHERi PTHR13832. PTHR13832. 1 hit.
Pfami PF00481. PP2C. 2 hits.
[Graphical view ]
SMARTi SM00332. PP2Cc. 1 hit.
[Graphical view ]
SUPFAMi SSF81606. SSF81606. 3 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "POLTERGEIST encodes a protein phosphatase 2C that regulates CLAVATA pathways controlling stem cell identity at Arabidopsis shoot and flower meristems."
    Yu L.P., Miller A.K., Clark S.E.
    Curr. Biol. 13:179-188(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF GLU-287 AND 443-GLN--ARG-856, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, FUNCTION, ENZYME REGULATION.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "POLTERGEIST functions to regulate meristem development downstream of the CLAVATA loci."
    Yu L.P., Simon E.J., Trotochaud A.E., Clark S.E.
    Development 127:1661-1670(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Large-scale analysis of in vivo phosphorylated membrane proteins by immobilized metal ion affinity chromatography and mass spectrometry."
    Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.
    Mol. Cell. Proteomics 2:1234-1243(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-152 AND SER-189, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: cv. La-0.
  7. "Phosphoproteomics of the Arabidopsis plasma membrane and a new phosphorylation site database."
    Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.
    Plant Cell 16:2394-2405(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-152 AND SER-189, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "POL and related phosphatases are dosage-sensitive regulators of meristem and organ development in Arabidopsis."
    Song S.-K., Clark S.E.
    Dev. Biol. 285:272-284(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE, DISRUPTION PHENOTYPE.
  9. "POL and PLL1 phosphatases are CLAVATA1 signaling intermediates required for Arabidopsis shoot and floral stem cells."
    Song S.-K., Lee M.M., Clark S.E.
    Development 133:4691-4698(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
    Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
    BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiP2C32_ARATH
AccessioniPrimary (citable) accession number: Q8RWN7
Secondary accession number(s): O80732
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: May 3, 2011
Last modified: October 29, 2014
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3