Q8RWN7 (P2C32_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein phosphatase 2C 32
Short name=AtPP2C32
EC=3.1.3.16

Alternative name(s):
Protein POLTERGEIST
Protein phosphatase 2C POL
Short name=PP2C POL

Gene names

Name:	POL
Ordered Locus Names:	At2g46920
ORF Names:	F14M4.25

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	856 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the regulation of pedicel length and of CLAVATA pathways controlling stem cell identity at shoot and flower meristems. Ref.1 Ref.7 Ref.8 Ref.9
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Enzyme regulation	Insensitive to okadaic acid. Ref.1
Subcellular location	Nucleus Probable.
Tissue specificity	Expressed in roots, leaves, stems, inflorescences, flowers and throughout the shoot meristem. Ref.1 Ref.8
Developmental stage	Expressed throughout development of the floral meristem, in all four floral whorls and as floral organs matured, limited to the ovule. Ref.1
Domain	The N-terminal domain (1-233) has a regulatory function and inhibits phosphatase activity. The conserved PP2C phosphatase domain (271-833) is interrupted by an insertion of approximately 200 amino acids.
Disruption phenotype	Loss-of-function mutant pol-6 (T-DNA insertion) shows suppression of clavata mutant phenotypes. The catalytic activity of POL may be functionally replaced by the activity of PLL1. Double mutant in POL and PLL1 is seedling lethal. Ref.8
Sequence similarities	Belongs to the PP2C family. Contains 1 PP2C-like domain.
Sequence caution	The sequence AAC34239.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Nucleus
Ligand	Magnesium Manganese Metal-binding
Molecular function	Developmental protein Hydrolase Protein phosphatase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	maintenance of meristem identity Inferred from genetic interaction Ref.9. Source: TAIR regulation of meristem structural organization Inferred from genetic interaction Ref.7. Source: TAIR regulation of transcription, DNA-dependent Inferred from genetic interaction Ref.9. Source: TAIR
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay Ref.5PubMed 17317660PubMed 20348433. Source: TAIR
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phospholipid binding Inferred from direct assay PubMed 20348433. Source: TAIR protein serine/threonine phosphatase activity Inferred from direct assay Ref.1. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 856	856	Protein phosphatase 2C 32		PRO_0000301258
Regions
	Domain	433 – 807	375	PP2C-like
Sites
	Metal binding	307	1	Manganese 1 By similarity
	Metal binding	307	1	Manganese 2 By similarity
	Metal binding	308	1	Manganese 1; via carbonyl oxygen By similarity
	Metal binding	763	1	Manganese 2 By similarity
	Metal binding	826	1	Manganese 2 By similarity
Amino acid modifications
	Modified residue	152	1	Phosphoserine Ref.6
	Modified residue	169	1	Phosphoserine Ref.5 Ref.6
	Modified residue	180	1	Phosphoserine Ref.6
	Modified residue	189	1	Phosphoserine Ref.6
Experimental info
	Mutagenesis	287	1	E → K in pol-1; reduced catalytic activity and partial phenotypic suppression of mutations within CLAVATA. Ref.1
	Mutagenesis	443 – 856	414	Missing in pol-3; weak phenotypic suppression of mutations within CLAVATA. Ref.1
	Sequence conflict	769	1	F → Y in AAM12971. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q8RWN7 [UniParc]. Last modified May 3, 2011. Version 2. Checksum: 95BDFC8DEB5DE4F2	FASTA	856	95,571
	10 20 30 40 50 60 MGNGTSRVVG CFVPSNDKNG VDLEFLEPLD EGLGHSFCYV RPSIFESPDI TPSNSERFTI 70 80 90 100 110 120 DSSTIDSETL TGSFRNDIVD DPSFLNRHNS KGLAETTFKA ISGASVSANV STARTGNQMA 130 140 150 160 170 180 LCSSDVLEPA ASFESTSSFA SIPLQPLPRG GSGPLNGFMS GPLERGFASG PLDRNNGFMS 190 200 210 220 230 240 GPIEKGVMSG PLDVSDRSNF SAPLSFRRKK PRFQRFMRSV SGPMKSTLAR TFSRRSGGLS 250 260 270 280 290 300 WMHRFFLHPE TRVSWAVGKD GKLHGEDPES CLESNRNLQW AHGKAGEDRV HVVLSEEQGW 310 320 330 340 350 360 LFIGIYDGFS GPDAPDFVMS HLYKAIDKEL EGLLWDYEEP SEDNQLQPDQ EPPTEENMCD 370 380 390 400 410 420 PESISEQHSK SVVAESEEVM IDDISSLGNT DTQIADGPPG DSAGPGKKSM RLYELLQLEQ 430 440 450 460 470 480 WEGEEIGLKR YGGNVALNNM TNQVENPSTS GGGAGNDPCT TDRSALDGIP NSGQRHGTKK 490 500 510 520 530 540 SQISSKIRRM YQKQKSLRKK LFPWSYDWHR EEGICVEEKI VESSGPIRRR WSGTVDHDAV 550 560 570 580 590 600 LRAMARALES TEEAYMDMVE KSLDINPELA LMGSCVLVML MKDQDVYVMN VGDSRAILAQ 610 620 630 640 650 660 ERLHDRHSNP GFGNDEGIGH KSRSRESLVR IELDRISEES PIHNQATPIS VSNKNRDVTS 670 680 690 700 710 720 YRLKMRAVQL SSDHSTSVEE EIWRIRSEHP EDDQSILKDR VKGQLKVTRA FGAGFLKKPN 730 740 750 760 770 780 FNEALLEMFQ VEYIGTDPYI TCEPCTVHHR LTSSDRFMVL SSDGLYEYFS NEEVVAHVTW 790 800 810 820 830 840 FIENVPEGDP AQYLIAELLS RAATKNGMEF HDLLDIPQGD RRKYHDDVSV MVVSLEGRIW 850 RSSGQYYPER KQKFNR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"POLTERGEIST encodes a protein phosphatase 2C that regulates CLAVATA pathways controlling stem cell identity at Arabidopsis shoot and flower meristems." Yu L.P., Miller A.K., Clark S.E. Curr. Biol. 13:179-188(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF GLU-287 AND 443-GLN--ARG-856, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, FUNCTION, ENZYME REGULATION.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Large-scale analysis of in vivo phosphorylated membrane proteins by immobilized metal ion affinity chromatography and mass spectrometry." Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C. Mol. Cell. Proteomics 2:1234-1243(2003) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-169, MASS SPECTROMETRY. Strain: cv. La-0.
[6]	"Phosphoproteomics of the Arabidopsis plasma membrane and a new phosphorylation site database." Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C. Plant Cell 16:2394-2405(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-152; SER-169; SER-180 AND SER-189, MASS SPECTROMETRY.
[7]	"POLTERGEIST functions to regulate meristem development downstream of the CLAVATA loci." Yu L.P., Simon E.J., Trotochaud A.E., Clark S.E. Development 127:1661-1670(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"POL and related phosphatases are dosage-sensitive regulators of meristem and organ development in Arabidopsis." Song S.-K., Clark S.E. Dev. Biol. 285:272-284(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE, DISRUPTION PHENOTYPE.
[9]	"POL and PLL1 phosphatases are CLAVATA1 signaling intermediates required for Arabidopsis shoot and floral stem cells." Song S.-K., Lee M.M., Clark S.E. Development 133:4691-4698(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[10]	"Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis." Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y. BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC004411 Genomic DNA. Translation: AAC34239.1. Sequence problems. CP002685 Genomic DNA. Translation: AEC10772.1. CP002685 Genomic DNA. Translation: AEC10773.1. AY092972 mRNA. Translation: AAM12971.1.
IPI	IPI00540166.
PIR	T02195.
RefSeq	NP_850463.1. NM_180132.2. NP_850464.1. NM_180133.1.
UniGene	At.25596.
3D structure databases
ProteinModelPortal	Q8RWN7.
SMR	Q8RWN7. Positions 667-839.
ModBase	Search...
Proteomic databases
PaxDb	Q8RWN7.
PRIDE	Q8RWN7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G46920.1; AT2G46920.1; AT2G46920. AT2G46920.2; AT2G46920.2; AT2G46920.
GeneID	819306.
KEGG	ath:AT2G46920.
Organism-specific databases
TAIR	At2g46920.
Phylogenomic databases
eggNOG	NOG267184.
HOGENOM	HOG000239375.
InParanoid	Q8RWN7.
OMA	FNDRVKG.
Gene expression databases
ArrayExpress	Q8RWN7.
Genevestigator	Q8RWN7.
Family and domain databases
Gene3D	3.60.40.10. 3 hits.
InterPro	IPR001932. PP2C-like. IPR015655. Protein_Pase_2C. [Graphical view]
PANTHER	PTHR13832. PTHR13832. 1 hit.
Pfam	PF00481. PP2C. 2 hits. [Graphical view]
SMART	SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF81606. PP2C-related. 1 hit.
PROSITE	PS01032. PP2C. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

P2C32_ARATH

Accession

Primary (citable) accession number: Q8RWN7
Secondary accession number(s): O80732

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	May 3, 2011
Last modified:	May 1, 2013
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program