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Q8RWN7 (P2C32_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein phosphatase 2C 32

Short name=AtPP2C32
EC=3.1.3.16
Alternative name(s):
Protein POLTERGEIST
Protein phosphatase 2C POL
Short name=PP2C POL
Gene names
Name:POL
Ordered Locus Names:At2g46920
ORF Names:F14M4.25
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length856 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the regulation of pedicel length and of CLAVATA pathways controlling stem cell identity at shoot and flower meristems. Ref.1 Ref.5 Ref.8 Ref.9

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Enzyme regulation

Insensitive to okadaic acid. Ref.1

Subcellular location

Nucleus Probable.

Tissue specificity

Expressed in roots, leaves, stems, inflorescences, flowers and throughout the shoot meristem. Ref.1 Ref.8

Developmental stage

Expressed throughout development of the floral meristem, in all four floral whorls and as floral organs matured, limited to the ovule. Ref.1

Domain

The N-terminal domain (1-233) has a regulatory function and inhibits phosphatase activity.

The conserved PP2C phosphatase domain (271-833) is interrupted by an insertion of approximately 200 amino acids.

Disruption phenotype

Loss-of-function mutant pol-6 (T-DNA insertion) shows suppression of clavata mutant phenotypes. The catalytic activity of POL may be functionally replaced by the activity of PLL1. Pol and pll1 double mutant is seedling lethal. Ref.8

Sequence similarities

Belongs to the PP2C family.

Contains 1 PP2C-like domain.

Sequence caution

The sequence AAC34239.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 856856Protein phosphatase 2C 32
PRO_0000301258

Regions

Domain433 – 807375PP2C-like

Sites

Metal binding3071Manganese 1 By similarity
Metal binding3071Manganese 2 By similarity
Metal binding3081Manganese 1; via carbonyl oxygen By similarity
Metal binding7631Manganese 2 By similarity
Metal binding8261Manganese 2 By similarity

Amino acid modifications

Modified residue1521Phosphoserine Ref.6 Ref.7
Modified residue1891Phosphoserine Ref.6 Ref.7
Modified residue2011Phosphoserine By similarity

Experimental info

Mutagenesis2871E → K in pol-1; reduced catalytic activity and partial phenotypic suppression of mutations within CLAVATA. Ref.1
Mutagenesis443 – 856414Missing in pol-3; weak phenotypic suppression of mutations within CLAVATA. Ref.1
Sequence conflict7691F → Y in AAM12971. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q8RWN7 [UniParc].

Last modified May 3, 2011. Version 2.
Checksum: 95BDFC8DEB5DE4F2

FASTA85695,571
        10         20         30         40         50         60 
MGNGTSRVVG CFVPSNDKNG VDLEFLEPLD EGLGHSFCYV RPSIFESPDI TPSNSERFTI 

        70         80         90        100        110        120 
DSSTIDSETL TGSFRNDIVD DPSFLNRHNS KGLAETTFKA ISGASVSANV STARTGNQMA 

       130        140        150        160        170        180 
LCSSDVLEPA ASFESTSSFA SIPLQPLPRG GSGPLNGFMS GPLERGFASG PLDRNNGFMS 

       190        200        210        220        230        240 
GPIEKGVMSG PLDVSDRSNF SAPLSFRRKK PRFQRFMRSV SGPMKSTLAR TFSRRSGGLS 

       250        260        270        280        290        300 
WMHRFFLHPE TRVSWAVGKD GKLHGEDPES CLESNRNLQW AHGKAGEDRV HVVLSEEQGW 

       310        320        330        340        350        360 
LFIGIYDGFS GPDAPDFVMS HLYKAIDKEL EGLLWDYEEP SEDNQLQPDQ EPPTEENMCD 

       370        380        390        400        410        420 
PESISEQHSK SVVAESEEVM IDDISSLGNT DTQIADGPPG DSAGPGKKSM RLYELLQLEQ 

       430        440        450        460        470        480 
WEGEEIGLKR YGGNVALNNM TNQVENPSTS GGGAGNDPCT TDRSALDGIP NSGQRHGTKK 

       490        500        510        520        530        540 
SQISSKIRRM YQKQKSLRKK LFPWSYDWHR EEGICVEEKI VESSGPIRRR WSGTVDHDAV 

       550        560        570        580        590        600 
LRAMARALES TEEAYMDMVE KSLDINPELA LMGSCVLVML MKDQDVYVMN VGDSRAILAQ 

       610        620        630        640        650        660 
ERLHDRHSNP GFGNDEGIGH KSRSRESLVR IELDRISEES PIHNQATPIS VSNKNRDVTS 

       670        680        690        700        710        720 
YRLKMRAVQL SSDHSTSVEE EIWRIRSEHP EDDQSILKDR VKGQLKVTRA FGAGFLKKPN 

       730        740        750        760        770        780 
FNEALLEMFQ VEYIGTDPYI TCEPCTVHHR LTSSDRFMVL SSDGLYEYFS NEEVVAHVTW 

       790        800        810        820        830        840 
FIENVPEGDP AQYLIAELLS RAATKNGMEF HDLLDIPQGD RRKYHDDVSV MVVSLEGRIW 

       850 
RSSGQYYPER KQKFNR 

« Hide

References

« Hide 'large scale' references
[1]"POLTERGEIST encodes a protein phosphatase 2C that regulates CLAVATA pathways controlling stem cell identity at Arabidopsis shoot and flower meristems."
Yu L.P., Miller A.K., Clark S.E.
Curr. Biol. 13:179-188(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF GLU-287 AND 443-GLN--ARG-856, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, FUNCTION, ENZYME REGULATION.
[2]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"POLTERGEIST functions to regulate meristem development downstream of the CLAVATA loci."
Yu L.P., Simon E.J., Trotochaud A.E., Clark S.E.
Development 127:1661-1670(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Large-scale analysis of in vivo phosphorylated membrane proteins by immobilized metal ion affinity chromatography and mass spectrometry."
Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.
Mol. Cell. Proteomics 2:1234-1243(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-152 AND SER-189, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: cv. La-0.
[7]"Phosphoproteomics of the Arabidopsis plasma membrane and a new phosphorylation site database."
Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.
Plant Cell 16:2394-2405(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-152 AND SER-189, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"POL and related phosphatases are dosage-sensitive regulators of meristem and organ development in Arabidopsis."
Song S.-K., Clark S.E.
Dev. Biol. 285:272-284(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE, DISRUPTION PHENOTYPE.
[9]"POL and PLL1 phosphatases are CLAVATA1 signaling intermediates required for Arabidopsis shoot and floral stem cells."
Song S.-K., Lee M.M., Clark S.E.
Development 133:4691-4698(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[10]"Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC004411 Genomic DNA. Translation: AAC34239.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC10772.1.
CP002685 Genomic DNA. Translation: AEC10773.1.
AY092972 mRNA. Translation: AAM12971.1.
PIRT02195.
RefSeqNP_850463.1. NM_180132.2.
NP_850464.1. NM_180133.1.
UniGeneAt.25596.

3D structure databases

ProteinModelPortalQ8RWN7.
SMRQ8RWN7. Positions 667-839.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ8RWN7. 1 interaction.
MINTMINT-8390824.

Proteomic databases

PaxDbQ8RWN7.
PRIDEQ8RWN7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G46920.1; AT2G46920.1; AT2G46920.
AT2G46920.2; AT2G46920.2; AT2G46920.
GeneID819306.
KEGGath:AT2G46920.

Organism-specific databases

TAIRAT2G46920.

Phylogenomic databases

eggNOGNOG267184.
HOGENOMHOG000239375.
InParanoidQ8RWN7.
OMAKGDLRHR.

Enzyme and pathway databases

BioCycARA:AT2G46920-MONOMER.
ARA:GQT-475-MONOMER.

Gene expression databases

GenevestigatorQ8RWN7.

Family and domain databases

Gene3D3.60.40.10. 3 hits.
InterProIPR001932. PP2C-like_dom.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERPTHR13832. PTHR13832. 1 hit.
PfamPF00481. PP2C. 2 hits.
[Graphical view]
SMARTSM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMSSF81606. SSF81606. 3 hits.
ProtoNetSearch...

Entry information

Entry nameP2C32_ARATH
AccessionPrimary (citable) accession number: Q8RWN7
Secondary accession number(s): O80732
Entry history
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: May 3, 2011
Last modified: April 16, 2014
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names