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Protein

CBL-interacting serine/threonine-protein kinase 1

Gene

CIPK1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

CIPK serine-threonine protein kinases interact with CBL proteins. Binding of a CBL protein to the regulatory NAF domain of CIPK protein lead to the activation of the kinase in a calcium-dependent manner.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Cofactori

Mn2+1 Publication

Kineticsi

  1. KM=15.2 µM for synthetic substrate1 Publication
  1. Vmax=146.1 pmol/min/mg enzyme1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei49 – 491ATPPROSITE-ProRule annotation
Active sitei143 – 1431Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi26 – 349ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • intracellular signal transduction Source: GO_Central
  • protein phosphorylation Source: GO_Central
  • response to abscisic acid Source: TAIR
  • response to osmotic stress Source: TAIR
  • response to salt stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT3G17510-MONOMER.
ARA:GQT-1565-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
CBL-interacting serine/threonine-protein kinase 1 (EC:2.7.11.1)
Alternative name(s):
SNF1-related kinase 3.16
SOS2-like protein kinase PKS13
Gene namesi
Name:CIPK1
Synonyms:PKS13, SnRK3.16
Ordered Locus Names:At3g17510
ORF Names:MKP6.6
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G17510.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • plasma membrane Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 444444CBL-interacting serine/threonine-protein kinase 1PRO_0000085861Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei165 – 1651PhosphoserineBy similarity
Modified residuei179 – 1791PhosphothreonineBy similarity

Post-translational modificationi

Autophosphorylated.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8RWC9.
PRIDEiQ8RWC9.

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

GenevisibleiQ8RWC9. AT.

Interactioni

Subunit structurei

Interacts with CBL1, CBL2, CBL3 and CBL9.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CBL1O8144510EBI-1748677,EBI-974530
CBL2Q8LAS74EBI-1748677,EBI-485991
CBL3Q8LEM74EBI-1748677,EBI-637358
CBL4O812233EBI-1748677,EBI-537541
ECT1Q3MK944EBI-1748677,EBI-2368594

Protein-protein interaction databases

BioGridi6349. 18 interactions.
IntActiQ8RWC9. 9 interactions.
MINTiMINT-8064946.
STRINGi3702.AT3G17510.1.

Structurei

3D structure databases

ProteinModelPortaliQ8RWC9.
SMRiQ8RWC9. Positions 16-429.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini20 – 275256Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini313 – 33725NAFPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni161 – 19030Activation loopBy similarityAdd
BLAST
Regioni343 – 37230PPIBy similarityAdd
BLAST

Domaini

The activation loop within the kinase domain is the target of phosphorylation/activation by upstream protein kinases. The PPI motif mediates the interaction with the ABI (abscisic acid-insensitive) phosphatases (By similarity).By similarity

Sequence similaritiesi

Contains 1 NAF domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0583. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000233016.
InParanoidiQ8RWC9.
OMAiDCSLYRQ.
PhylomeDBiQ8RWC9.

Family and domain databases

Gene3Di3.30.310.80. 1 hit.
InterProiIPR028375. KA1/Ssp2_C.
IPR011009. Kinase-like_dom.
IPR018451. NAF/FISL_domain.
IPR004041. NAF_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF03822. NAF. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50816. NAF. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8RWC9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVRRQEEEKK AEKGMRLGKY ELGRTLGEGN FGKVKFAKDT VSGHSFAVKI
60 70 80 90 100
IDKSRIADLN FSLQIKREIR TLKMLKHPHI VRLHEVLASK TKINMVMELV
110 120 130 140 150
TGGELFDRIV SNGKLTETDG RKMFQQLIDG ISYCHSKGVF HRDLKLENVL
160 170 180 190 200
LDAKGHIKIT DFGLSALPQH FRDDGLLHTT CGSPNYVAPE VLANRGYDGA
210 220 230 240 250
ASDIWSCGVI LYVILTGCLP FDDRNLAVLY QKICKGDPPI PRWLSPGART
260 270 280 290 300
MIKRMLDPNP VTRITVVGIK ASEWFKLEYI PSIPDDDDEE EVDTDDDAFS
310 320 330 340 350
IQELGSEEGK GSDSPTIINA FQLIGMSSFL DLSGFFEQEN VSERRIRFTS
360 370 380 390 400
NSSAKDLLEK IETAVTEMGF SVQKKHAKLR VKQEERNQKG QVGLSVTAEV
410 420 430 440
FEIKPSLNVV ELRKSYGDSC LYRQLYERLL KDVGTSSPEQ EIVT
Length:444
Mass (Da):49,933
Last modified:December 21, 2004 - v2
Checksum:i63DB8AF69CCAA5C9
GO
Isoform 2 (identifier: Q8RWC9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-80: Missing.
     81-85: VRLHE → MVIMQ

Note: May be due to intron retention. No experimental confirmation available.
Show »
Length:364
Mass (Da):40,679
Checksum:i31BC492529353667
GO

Sequence cautioni

The sequence BAB02040.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti179 – 1791T → N in AAG28776 (PubMed:10590166).Curated
Sequence conflicti237 – 2371D → N in AAM13176 (PubMed:14593172).Curated
Sequence conflicti382 – 3821K → N in AAG28776 (PubMed:10590166).Curated
Sequence conflicti387 – 3871N → T in AAG28776 (PubMed:10590166).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 8080Missing in isoform 2. 1 PublicationVSP_026063Add
BLAST
Alternative sequencei81 – 855VRLHE → MVIMQ in isoform 2. 1 PublicationVSP_026064

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF302112 mRNA. Translation: AAG28776.1.
AB022219 Genomic DNA. Translation: BAB02040.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE75962.1.
CP002686 Genomic DNA. Translation: AEE75963.1.
AY093177 mRNA. Translation: AAM13176.1.
BT029495 mRNA. Translation: ABL66752.1.
RefSeqiNP_566580.1. NM_112631.2. [Q8RWC9-1]
NP_974328.1. NM_202599.1. [Q8RWC9-2]
UniGeneiAt.21023.

Genome annotation databases

EnsemblPlantsiAT3G17510.1; AT3G17510.1; AT3G17510. [Q8RWC9-1]
GeneIDi821016.
KEGGiath:AT3G17510.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF302112 mRNA. Translation: AAG28776.1.
AB022219 Genomic DNA. Translation: BAB02040.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE75962.1.
CP002686 Genomic DNA. Translation: AEE75963.1.
AY093177 mRNA. Translation: AAM13176.1.
BT029495 mRNA. Translation: ABL66752.1.
RefSeqiNP_566580.1. NM_112631.2. [Q8RWC9-1]
NP_974328.1. NM_202599.1. [Q8RWC9-2]
UniGeneiAt.21023.

3D structure databases

ProteinModelPortaliQ8RWC9.
SMRiQ8RWC9. Positions 16-429.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi6349. 18 interactions.
IntActiQ8RWC9. 9 interactions.
MINTiMINT-8064946.
STRINGi3702.AT3G17510.1.

Proteomic databases

PaxDbiQ8RWC9.
PRIDEiQ8RWC9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G17510.1; AT3G17510.1; AT3G17510. [Q8RWC9-1]
GeneIDi821016.
KEGGiath:AT3G17510.

Organism-specific databases

TAIRiAT3G17510.

Phylogenomic databases

eggNOGiKOG0583. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000233016.
InParanoidiQ8RWC9.
OMAiDCSLYRQ.
PhylomeDBiQ8RWC9.

Enzyme and pathway databases

BioCyciARA:AT3G17510-MONOMER.
ARA:GQT-1565-MONOMER.

Miscellaneous databases

PROiQ8RWC9.

Gene expression databases

GenevisibleiQ8RWC9. AT.

Family and domain databases

Gene3Di3.30.310.80. 1 hit.
InterProiIPR028375. KA1/Ssp2_C.
IPR011009. Kinase-like_dom.
IPR018451. NAF/FISL_domain.
IPR004041. NAF_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF03822. NAF. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50816. NAF. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Novel protein kinases associated with calcineurin B-like calcium sensors in Arabidopsis."
    Shi J., Kim K.-N., Ritz O., Albrecht V., Gupta R., Harter K., Luan S., Kudla J.
    Plant Cell 11:2393-2405(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, INTERACTION WITH CBL1.
  2. "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
    Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
    DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  5. "Arabidopsis ORF clones."
    Bautista V.R., Kim C.J., Chen H., Quinitio C., Ecker J.R.
    Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Columbia.
  6. "Interaction specificity of Arabidopsis calcineurin B-like calcium sensors and their target kinases."
    Kim K.-N., Cheong Y.H., Gupta R., Luan S.
    Plant Physiol. 124:1844-1853(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CBL1 AND CBL3.
    Strain: cv. Columbia.
  7. "The NAF domain defines a novel protein-protein interaction module conserved in Ca(2+)-regulated kinases."
    Albrecht V., Ritz O., Linder S., Harter K., Kudla J.
    EMBO J. 20:1051-1063(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CBL2 AND CBL3.
  8. Cited for: GENE FAMILY, NOMENCLATURE.
  9. "Calcium sensors and their interacting protein kinases: genomics of the Arabidopsis and rice CBL-CIPK signaling networks."
    Kolukisaoglu U., Weinl S., Blazevic D., Batistic O., Kudla J.
    Plant Physiol. 134:43-58(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CBL1 AND CBL9.
  10. "Phosphorylation of calcineurin B-like (CBL) calcium sensor proteins by their CBL-interacting protein kinases (CIPKs) is required for full activity of CBL-CIPK complexes toward their target proteins."
    Hashimoto K., Eckert C., Anschuetz U., Scholz M., Held K., Waadt R., Reyer A., Hippler M., Becker D., Kudla J.
    J. Biol. Chem. 287:7956-7968(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, PHOSPHORYLATION.

Entry informationi

Entry nameiCIPK1_ARATH
AccessioniPrimary (citable) accession number: Q8RWC9
Secondary accession number(s): Q3EB49, Q9FUK2, Q9LUP6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: December 21, 2004
Last modified: May 11, 2016
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.