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Protein

Nicotinamide adenine dinucleotide transporter 2, mitochondrial

Gene

NDT2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Mediates the NAD+ import into chloroplast. Favors the NAD+(in)/ADP or AMP(out) antiport exchange, but is also able to catalyze a low unidirectional transport (uniport) of NAD+. Transports NAD+, nicotinic acid adenine dinucleotide, nicotinamide mononucleotide, nicotinic acid mononucleotide, FAD, FMN, TTP, TDP, TMP, UTP, UDP, UMP, CTP, CDP, CMP, GTP, GDP, GMP, 3'-AMP, ATP, ADP, AND AMP, has low transport activity with cAMP, NADH and alpha-NAD+, and has no activity with NADP+, NADPH, nicotinamide, nicotinic acid, adenosine, thiamine mono- or diphosphate, inorganic phosphate, CoA, folate, NaCl, malate, malonate, citrate, fumarate, aspartate, glutamate, S-adenosylmethionine, lysine, arginine, and ornithine.2 Publications

Miscellaneous

Appears to be a mitochondrial envelope-located membrane protein lacking an N-terminal-located transit peptide.

Enzyme regulationi

Inhibited by pyridoxal 5'-phosphate, bathophenanthroline, tannic acid, mersalyl, mercuric chloride, p-hydroxymercuribenzoate, p-hydroxymercuribenzoate sulfonate, bromocresol purple and N-ethylmaleimide.1 Publication

Kineticsi

  1. KM=0.15 mM for for the NAD+/NAD+ exchange1 Publication
  1. Vmax=4.76 mmol/min/g enzyme for the NAD+/NAD+ exchange1 Publication

GO - Molecular functioni

  • antiporter activity Source: UniProtKB-KW
  • NAD transporter activity Source: TAIR
  • transmembrane transporter activity Source: GO_Central

GO - Biological processi

  • mitochondrial transport Source: GO_Central
  • NAD transmembrane transport Source: GO_Central
  • NAD transport Source: TAIR

Keywordsi

Biological processAntiport, Transport

Protein family/group databases

TCDBi2.A.29.10.10. the mitochondrial carrier (mc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Nicotinamide adenine dinucleotide transporter 2, mitochondrial
Short name:
AtNDT2
Alternative name(s):
NAD(+) transporter 2
Gene namesi
Name:NDT2
Synonyms:NADT2
Ordered Locus Names:At1g25380
ORF Names:F4F7.45
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G25380.
TAIRilocus:2031240. AT1G25380.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei21 – 41Helical; Name=1Sequence analysisAdd BLAST21
Transmembranei82 – 102Helical; Name=2Sequence analysisAdd BLAST21
Transmembranei121 – 141Helical; Name=3Sequence analysisAdd BLAST21
Transmembranei176 – 196Helical; Name=4Sequence analysisAdd BLAST21
Transmembranei215 – 235Helical; Name=5Sequence analysisAdd BLAST21
Transmembranei277 – 299Helical; Name=6Sequence analysisAdd BLAST23

GO - Cellular componenti

Keywords - Cellular componenti

Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004206961 – 363Nicotinamide adenine dinucleotide transporter 2, mitochondrialAdd BLAST363

Proteomic databases

PaxDbiQ8RWA5.

Expressioni

Tissue specificityi

Highly expressed in young meristematic shoot area, vascular bundles of leaves, developing siliques including the funiculi, petal veins, developing pollen and central cylinder of roots.1 Publication

Gene expression databases

ExpressionAtlasiQ8RWA5. baseline and differential.
GenevisibleiQ8RWA5. AT.

Interactioni

Protein-protein interaction databases

BioGridi24361. 1 interactor.
STRINGi3702.AT1G25380.1.

Structurei

3D structure databases

ProteinModelPortaliQ8RWA5.
SMRiQ8RWA5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati15 – 107Solcar 1Add BLAST93
Repeati115 – 203Solcar 2Add BLAST89
Repeati215 – 305Solcar 3Add BLAST91

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0757. Eukaryota.
ENOG410XS20. LUCA.
HOGENOMiHOG000188210.
InParanoidiQ8RWA5.
KOiK15115.
OMAiANCSSHE.
OrthoDBiEOG09360FC7.
PhylomeDBiQ8RWA5.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiView protein in InterPro
IPR002067. Mit_carrier.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
PfamiView protein in Pfam
PF00153. Mito_carr. 3 hits.
PRINTSiPR00926. MITOCARRIER.
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiView protein in PROSITE
PS50920. SOLCAR. 3 hits.

Sequencei

Sequence statusi: Complete.

Q8RWA5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIEHGNSTFD YRSIREVAAN AGAGATAGAI AATFVCPLDV IKTRLQVLGL
60 70 80 90 100
PEAPASGQRG GVIITSLKNI IKEEGYRGMY RGLSPTIIAL LPNWAVYFSV
110 120 130 140 150
YGKLKDVLQS SDGKLSIGSN MIAAAGAGAA TSIATNPLWV VKTRLMTQGI
160 170 180 190 200
RPGVVPYKSV MSAFSRICHE EGVRGLYSGI LPSLAGVSHV AIQFPAYEKI
210 220 230 240 250
KQYMAKMDNT SVENLSPGNV AIASSIAKVI ASILTYPHEV IRAKLQEQGQ
260 270 280 290 300
IRNAETKYSG VIDCITKVFR SEGIPGLYRG CATNLLRTTP SAVITFTTYE
310 320 330 340 350
MMLRFFRQVV PPETNRSDDR RREEERKNLV SRRGEEEDKD LGLRESQTQS
360
NKISTPHIPL GSK
Length:363
Mass (Da):39,496
Last modified:June 1, 2002 - v1
Checksum:i44FA388F224EE992
GO

Sequence cautioni

The sequence AAG28807 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAG50815 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti84S → L in CAI38581 (PubMed:16055441).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ871010 mRNA. Translation: CAI38581.1.
FM211594 mRNA. Translation: CAR70089.1.
AC079281 Genomic DNA. Translation: AAG50815.1. Sequence problems.
AC079374 Genomic DNA. Translation: AAG28807.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE30615.1.
CP002684 Genomic DNA. Translation: ANM58659.1.
AY093232 mRNA. Translation: AAM13231.1.
BT008746 mRNA. Translation: AAP42759.1.
PIRiG86383.
RefSeqiNP_001321077.1. NM_001332677.1.
NP_564233.1. NM_102349.4.
UniGeneiAt.41349.

Genome annotation databases

EnsemblPlantsiAT1G25380.1; AT1G25380.1; AT1G25380.
AT1G25380.2; AT1G25380.2; AT1G25380.
GeneIDi839124.
GrameneiAT1G25380.1; AT1G25380.1; AT1G25380.
AT1G25380.2; AT1G25380.2; AT1G25380.
KEGGiath:AT1G25380.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiNDT2_ARATH
AccessioniPrimary (citable) accession number: Q8RWA5
Secondary accession number(s): Q4A3J5, Q9C6K8, Q9FRI7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 9, 2013
Last sequence update: June 1, 2002
Last modified: June 7, 2017
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families