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Protein

Nicotinamide adenine dinucleotide transporter 2, mitochondrial

Gene

NDT2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Mediates the NAD+ import into chloroplast. Favors the NAD+(in)/ADP or AMP(out) antiport exchange, but is also able to catalyze a low unidirectional transport (uniport) of NAD+. Transports NAD+, nicotinic acid adenine dinucleotide, nicotinamide mononucleotide, nicotinic acid mononucleotide, FAD, FMN, TTP, TDP, TMP, UTP, UDP, UMP, CTP, CDP, CMP, GTP, GDP, GMP, 3'-AMP, ATP, ADP, AND AMP, has low transport activity with cAMP, NADH and alpha-NAD+, and has no activity with NADP+, NADPH, nicotinamide, nicotinic acid, adenosine, thiamine mono- or diphosphate, inorganic phosphate, CoA, folate, NaCl, malate, malonate, citrate, fumarate, aspartate, glutamate, S-adenosylmethionine, lysine, arginine, and ornithine.2 Publications

Enzyme regulationi

Inhibited by pyridoxal 5'-phosphate, bathophenanthroline, tannic acid, mersalyl, mercuric chloride, p-hydroxymercuribenzoate, p-hydroxymercuribenzoate sulfonate, bromocresol purple and N-ethylmaleimide.1 Publication

Kineticsi

  1. KM=0.15 mM for for the NAD+/NAD+ exchange1 Publication
  1. Vmax=4.76 mmol/min/g enzyme for the NAD+/NAD+ exchange1 Publication

GO - Molecular functioni

  • antiporter activity Source: UniProtKB-KW
  • NAD transporter activity Source: TAIR
  • structural constituent of ribosome Source: GO_Central

GO - Biological processi

  • NAD transport Source: TAIR
  • translation Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Antiport, Transport

Protein family/group databases

TCDBi2.A.29.10.10. the mitochondrial carrier (mc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Nicotinamide adenine dinucleotide transporter 2, mitochondrial
Short name:
AtNDT2
Alternative name(s):
NAD(+) transporter 2
Gene namesi
Name:NDT2
Synonyms:NADT2
Ordered Locus Names:At1g25380
ORF Names:F4F7.45
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G25380.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei21 – 4121Helical; Name=1Sequence analysisAdd
BLAST
Transmembranei82 – 10221Helical; Name=2Sequence analysisAdd
BLAST
Transmembranei121 – 14121Helical; Name=3Sequence analysisAdd
BLAST
Transmembranei176 – 19621Helical; Name=4Sequence analysisAdd
BLAST
Transmembranei215 – 23521Helical; Name=5Sequence analysisAdd
BLAST
Transmembranei277 – 29923Helical; Name=6Sequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 363363Nicotinamide adenine dinucleotide transporter 2, mitochondrialPRO_0000420696Add
BLAST

Proteomic databases

PaxDbiQ8RWA5.
PRIDEiQ8RWA5.

Expressioni

Tissue specificityi

Highly expressed in young meristematic shoot area, vascular bundles of leaves, developing siliques including the funiculi, petal veins, developing pollen and central cylinder of roots.1 Publication

Gene expression databases

GenevisibleiQ8RWA5. AT.

Interactioni

Protein-protein interaction databases

BioGridi24361. 1 interaction.
STRINGi3702.AT1G25380.1.

Structurei

3D structure databases

ProteinModelPortaliQ8RWA5.
SMRiQ8RWA5. Positions 15-304.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati15 – 10793Solcar 1Add
BLAST
Repeati115 – 20389Solcar 2Add
BLAST
Repeati215 – 30591Solcar 3Add
BLAST

Sequence similaritiesi

Contains 3 Solcar repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0757. Eukaryota.
ENOG410XS20. LUCA.
HOGENOMiHOG000188210.
InParanoidiQ8RWA5.
KOiK15115.
OMAiQYAGVID.
OrthoDBiEOG09360FC7.
PhylomeDBiQ8RWA5.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR002067. Mit_carrier.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
PRINTSiPR00926. MITOCARRIER.
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8RWA5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIEHGNSTFD YRSIREVAAN AGAGATAGAI AATFVCPLDV IKTRLQVLGL
60 70 80 90 100
PEAPASGQRG GVIITSLKNI IKEEGYRGMY RGLSPTIIAL LPNWAVYFSV
110 120 130 140 150
YGKLKDVLQS SDGKLSIGSN MIAAAGAGAA TSIATNPLWV VKTRLMTQGI
160 170 180 190 200
RPGVVPYKSV MSAFSRICHE EGVRGLYSGI LPSLAGVSHV AIQFPAYEKI
210 220 230 240 250
KQYMAKMDNT SVENLSPGNV AIASSIAKVI ASILTYPHEV IRAKLQEQGQ
260 270 280 290 300
IRNAETKYSG VIDCITKVFR SEGIPGLYRG CATNLLRTTP SAVITFTTYE
310 320 330 340 350
MMLRFFRQVV PPETNRSDDR RREEERKNLV SRRGEEEDKD LGLRESQTQS
360
NKISTPHIPL GSK
Length:363
Mass (Da):39,496
Last modified:June 1, 2002 - v1
Checksum:i44FA388F224EE992
GO

Sequence cautioni

The sequence AAG28807 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence AAG50815 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti84 – 841S → L in CAI38581 (PubMed:16055441).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ871010 mRNA. Translation: CAI38581.1.
FM211594 mRNA. Translation: CAR70089.1.
AC079281 Genomic DNA. Translation: AAG50815.1. Sequence problems.
AC079374 Genomic DNA. Translation: AAG28807.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE30615.1.
AY093232 mRNA. Translation: AAM13231.1.
BT008746 mRNA. Translation: AAP42759.1.
PIRiG86383.
RefSeqiNP_564233.1. NM_102349.3.
UniGeneiAt.41349.

Genome annotation databases

EnsemblPlantsiAT1G25380.1; AT1G25380.1; AT1G25380.
GeneIDi839124.
GrameneiAT1G25380.1; AT1G25380.1; AT1G25380.
KEGGiath:AT1G25380.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ871010 mRNA. Translation: CAI38581.1.
FM211594 mRNA. Translation: CAR70089.1.
AC079281 Genomic DNA. Translation: AAG50815.1. Sequence problems.
AC079374 Genomic DNA. Translation: AAG28807.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE30615.1.
AY093232 mRNA. Translation: AAM13231.1.
BT008746 mRNA. Translation: AAP42759.1.
PIRiG86383.
RefSeqiNP_564233.1. NM_102349.3.
UniGeneiAt.41349.

3D structure databases

ProteinModelPortaliQ8RWA5.
SMRiQ8RWA5. Positions 15-304.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi24361. 1 interaction.
STRINGi3702.AT1G25380.1.

Protein family/group databases

TCDBi2.A.29.10.10. the mitochondrial carrier (mc) family.

Proteomic databases

PaxDbiQ8RWA5.
PRIDEiQ8RWA5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G25380.1; AT1G25380.1; AT1G25380.
GeneIDi839124.
GrameneiAT1G25380.1; AT1G25380.1; AT1G25380.
KEGGiath:AT1G25380.

Organism-specific databases

TAIRiAT1G25380.

Phylogenomic databases

eggNOGiKOG0757. Eukaryota.
ENOG410XS20. LUCA.
HOGENOMiHOG000188210.
InParanoidiQ8RWA5.
KOiK15115.
OMAiQYAGVID.
OrthoDBiEOG09360FC7.
PhylomeDBiQ8RWA5.

Miscellaneous databases

PROiQ8RWA5.

Gene expression databases

GenevisibleiQ8RWA5. AT.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR002067. Mit_carrier.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
PRINTSiPR00926. MITOCARRIER.
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNDT2_ARATH
AccessioniPrimary (citable) accession number: Q8RWA5
Secondary accession number(s): Q4A3J5, Q9C6K8, Q9FRI7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2013
Last sequence update: June 1, 2002
Last modified: September 7, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Appears to be a mitochondrial envelope-located membrane protein lacking an N-terminal-located transit peptide.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.