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Protein

Serine/threonine protein phosphatase 2A 59 kDa regulatory subunit B' gamma isoform

Gene

B'GAMMA

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The B regulatory subunit may modulate substrate selectivity and catalytic activity, and also may direct the localization of the catalytic enzyme to a particular subcellular compartment (By similarity). Required for the formation of the PP2A holoenzyme that negatively regulates brassinosteroid signaling by dephosphorylating and inactivating BRI1 in the cytoplasm (PubMed:26517938). Seems to be functionally connected with CPR5 and may mediate the negative regulation of defense reactions and senescence under low irradiances. May contribute to the epigenetic regulation of defense gene expression (PubMed:21571669). Involved in the control of methoxylation of indole glucosinolates and formation of 4-methoxy- indol-3-yl-methyl glucosinolate in leaves, through direct interaction with indole glucosinolate methyltransferases (PubMed:27598402). Involved in growth regulation and stress signaling (PubMed:26012558). Involved in the regulation of reactive oxygen species (ROS) signaling and maintenance of cellular ROS homeostasis (PubMed:26012558, PubMed:25307043). Required to control the level of ACO3 phosphorylation in the cytoplasm. Regulates hydrogen peroxide metabolism by controlling the abundance of AOX1A and AXO3/AOX1D in leaf mitochondria (PubMed:25307043). May mediate dephosphorylation of CRT1 and promote the degradation of unfolded proteins in endoplasmic reticulum (ER) (PubMed:22041935). Involved in the regulation of flowering time by repressing FLC, the main flowering repressor gene (PubMed:23976921).By similarity7 Publications

GO - Molecular functioni

  • poly(U) RNA binding Source: TAIR
  • protein phosphatase regulator activity Source: InterPro

GO - Biological processi

  • brassinosteroid mediated signaling pathway Source: UniProtKB-KW
  • defense response Source: UniProtKB-KW
  • flower development Source: UniProtKB-KW
  • indole glucosinolate biosynthetic process Source: TAIR
  • methionine metabolic process Source: TAIR
  • negative regulation of defense response Source: TAIR
  • regulation of cell aging Source: TAIR
  • regulation of reactive oxygen species metabolic process Source: UniProtKB
  • S-adenosylmethionine cycle Source: TAIR
  • trichome morphogenesis Source: TAIR

Keywordsi

Biological processBrassinosteroid signaling pathway, Flowering, Plant defense, Stress response

Enzyme and pathway databases

ReactomeiR-ATH-198753. ERK/MAPK targets.
R-ATH-202670. ERKs are inactivated.
R-ATH-5673000. RAF activation.
R-ATH-5675221. Negative regulation of MAPK pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine protein phosphatase 2A 59 kDa regulatory subunit B' gamma isoform
Short name:
AtB' gamma
Short name:
PP2A, B' subunit, gamma isoform
Gene namesi
Name:B'GAMMA
Ordered Locus Names:At4g15415
ORF Names:dl3750w, FCAALL.118
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G15415.
TAIRilocus:505006470. AT4G15415.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: UniProtKB-SubCell
  • nucleus Source: UniProtKB-SubCell
  • protein phosphatase type 2A complex Source: InterPro

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Wrinkled leaves, stunted growth, delayed flowering and formation of age-dependent yellowing lesions (PubMed:21571669, PubMed:22041935, PubMed:23976921). Conditional phenotype with premature yellowing and constitutive reactive oxygen species (ROS) production in distinct peripheral areas of mature leaves when grown under moderate light intensity and low humidity. May partially evade the accumulation of H2O2 via alternative oxidases (AOX) activity. Increased levels of AOX1A and AOX1D in leaf mitochondria. Increased level of ACO3 phosphorylation.4 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000714621 – 522Serine/threonine protein phosphatase 2A 59 kDa regulatory subunit B' gamma isoformAdd BLAST522

Proteomic databases

PaxDbiQ8RW96.

PTM databases

iPTMnetiQ8RW96.

Expressioni

Tissue specificityi

Expressed ubiquitously at low levels (PubMed:9128737). Expressed in roots, emerging lateral roots, cotyledons, leaves, floral stalks and flowers (PubMed:26012558).2 Publications

Inductioni

By heat shock.1 Publication

Gene expression databases

ExpressionAtlasiQ8RW96. baseline and differential.
GenevisibleiQ8RW96. AT.

Interactioni

Subunit structurei

PP2A consists of a common heteromeric enzyme, composed of a catalytic subunit (subunits C), a constant regulatory subunit (subunit A), and a variety of regulatory subunits such as subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families) (Probable). Interacts with BRI1 (PubMed:26517938). Interacts with IGMT1 and IGMT4 (PubMed:27598402). Interacts with ACO3 in the cytosol (PubMed:25307043).1 Publication3 Publications

Protein-protein interaction databases

BioGridi12508. 5 interactors.
STRINGi3702.AT4G15415.1.

Structurei

3D structure databases

ProteinModelPortaliQ8RW96.
SMRiQ8RW96.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi14 – 69Ser-richAdd BLAST56

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2085. Eukaryota.
ENOG410XQJW. LUCA.
HOGENOMiHOG000067326.
KOiK11584.
OMAiEGHHVPP.
OrthoDBiEOG0936077A.
PhylomeDBiQ8RW96.

Family and domain databases

InterProiView protein in InterPro
IPR016024. ARM-type_fold.
IPR002554. PP2A_B56.
PANTHERiPTHR10257. PTHR10257. 1 hit.
PfamiView protein in Pfam
PF01603. B56. 1 hit.
PIRSFiPIRSF028043. PP2A_B56. 1 hit.
SUPFAMiSSF48371. SSF48371. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8RW96-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKQIFGKLP RKPSKSSHND SNPNGEGGVN SYYIPNSGIS SISKPSSKSS
60 70 80 90 100
ASNSNGANGT VIAPSSTSSN RTNQVNGVYE ALPSFRDVPT SEKPNLFIKK
110 120 130 140 150
LSMCCVVFDF NDPSKNLREK EIKRQTLLEL VDYIATVSTK LSDAAMQEIA
160 170 180 190 200
KVAVVNLFRT FPSANHESKI LETLDVDDEE PALEPAWPHL QVVYELLLRF
210 220 230 240 250
VASPMTDAKL AKRYIDHSFV LKLLDLFDSE DQREREYLKT ILHRIYGKFM
260 270 280 290 300
VHRPFIRKAI NNIFYRFIFE TEKHNGIAEL LEILGSIING FALPLKEEHK
310 320 330 340 350
LFLIRALIPL HRPKCASAYH QQLSYCIVQF VEKDFKLADT VIRGLLKYWP
360 370 380 390 400
VTNSSKEVMF LGELEEVLEA TQAAEFQRCM VPLFRQIARC LNSSHFQVAE
410 420 430 440 450
RALFLWNNDH IRNLITQNHK VIMPIVFPAM ERNTRGHWNQ AVQSLTLNVR
460 470 480 490 500
KVMAETDQIL FDECLAKFQE DEANETEVVA KREATWKLLE ELAASKSVSN
510 520
EAVLVPRFSS SVTLATGKTS GS
Length:522
Mass (Da):59,157
Last modified:January 24, 2006 - v2
Checksum:iAACF6C94287760EA
GO

Sequence cautioni

The sequence CAB10320 differs from that shown. Reason: Erroneous gene model prediction. The predicted gene At4g15410 has been split into 2 genes: At4g15410 and At4g15415.Curated
The sequence CAB78583 differs from that shown. Reason: Erroneous gene model prediction. The predicted gene At4g15410 has been split into 2 genes: At4g15410 and At4g15415.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti384F → L in AAM10385 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73528 mRNA. Translation: AAB58902.1.
Z97338 Genomic DNA. Translation: CAB10320.1. Sequence problems.
AL161541 Genomic DNA. Translation: CAB78583.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE83598.1.
CP002687 Genomic DNA. Translation: AEE83599.1.
CP002687 Genomic DNA. Translation: ANM66523.1.
CP002687 Genomic DNA. Translation: ANM66524.1.
AY093761 mRNA. Translation: AAM10385.1.
BT020581 mRNA. Translation: AAW80854.1.
PIRiF71418.
RefSeqiNP_001319948.1. NM_001341040.1.
NP_001319949.1. NM_001341039.1.
NP_567464.1. NM_117630.3.
NP_849390.1. NM_179059.2.
UniGeneiAt.4343.

Genome annotation databases

EnsemblPlantsiAT4G15415.1; AT4G15415.1; AT4G15415.
AT4G15415.2; AT4G15415.2; AT4G15415.
AT4G15415.4; AT4G15415.4; AT4G15415.
AT4G15415.5; AT4G15415.5; AT4G15415.
GeneIDi827211.
GrameneiAT4G15415.1; AT4G15415.1; AT4G15415.
AT4G15415.2; AT4G15415.2; AT4G15415.
AT4G15415.4; AT4G15415.4; AT4G15415.
AT4G15415.5; AT4G15415.5; AT4G15415.
KEGGiath:AT4G15415.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry namei2A5G_ARATH
AccessioniPrimary (citable) accession number: Q8RW96
Secondary accession number(s): O04377, O23394
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: January 24, 2006
Last modified: June 7, 2017
This is version 98 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families