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Q8RVK9 (CHS_CANSA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Naringenin-chalcone synthase

EC=2.3.1.74
Gene names
Name:CHS
Synonyms:CAN1069
OrganismCannabis sativa (Hemp) (Marijuana)
Taxonomic identifier3483 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsRosalesCannabaceaeCannabis

Protein attributes

Sequence length389 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Chalcone synthase that can also use isovaleryl-CoA, isobutyryl-CoA or hexanoyl-CoA as substrates, but that is unable produce olivetol or olivetolic acid.

Catalytic activity

3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2. Ref.1 Ref.2

Subcellular location

Cytoplasm Probable.

Tissue specificity

Expressed in glandular trichomes. Detected at low levels in female flowers, stems, seeds, leaves and roots. Ref.1 Ref.2

Sequence similarities

Belongs to the chalcone/stilbene synthases family.

Ontologies

Keywords
   Cellular componentCytoplasm
   Molecular functionAcyltransferase
Transferase
Gene Ontology (GO)
   Biological_processcannabinoid biosynthetic process

Non-traceable author statement Ref.1. Source: UniProtKB

chalcone biosynthetic process

Inferred from direct assay Ref.1. Source: UniProtKB

   Cellular_componentpolyketide synthase complex

Inferred from direct assay Ref.1. Source: UniProtKB

   Molecular_functionnaringenin-chalcone synthase activity

Inferred from direct assay Ref.1. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 389389Naringenin-chalcone synthase
PRO_0000421153

Sites

Active site1641 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8RVK9 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 51C5C0C87EFF6279

FASTA38942,720
        10         20         30         40         50         60 
MVTVEEFRKA QRAEGPATIM AIGTATPANC VLQSEYPDYY FRITNSEHKT ELKEKFKRMC 

        70         80         90        100        110        120 
DKSMIRKRYM HLTEEILKEN PNLCAYEAPS LDARQDMVVV EVPKLGKEAA TKAIKEWGQP 

       130        140        150        160        170        180 
KSKITHLVFC TTSGVDMPGA DYQLTKLLGL RPSVKRLMMY QQGCFAGGTV LRLAKDLAEN 

       190        200        210        220        230        240 
NKGARVLVVC SEITAVTFRG PNDTHLDSLV GQALFGDGSA ALIVGSDPIP EVEKPIFELV 

       250        260        270        280        290        300 
SAAQTILPDS DGAIDGHLRE VGLTFHLLKD VPGLISKNIE KSLNEAFKPL GISDWNSLFW 

       310        320        330        340        350        360 
IAHPGGPAIL DQVESKLALK TEKLRATRHV LSEYGNMSSA CVLFILDEMR RKCVEDGLNT 

       370        380 
TGEGLEWGVL FGFGPGLTVE TVVLHSVAI 

« Hide

References

[1]"Cloning and over-expression of a cDNA encoding a polyketide synthase from Cannabis sativa."
Raharjo T.J., Chang W.T., Verberne M.C., Peltenburg-Looman A.M., Linthorst H.J., Verpoorte R.
Plant Physiol. Biochem. 42:291-297(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, TISSUE SPECIFICITY.
Strain: cv. Four-way.
Tissue: Leaf.
[2]"Identification of candidate genes affecting Delta9-tetrahydrocannabinol biosynthesis in Cannabis sativa."
Marks M.D., Tian L., Wenger J.P., Omburo S.N., Soto-Fuentes W., He J., Gang D.R., Weiblen G.D., Dixon R.A.
J. Exp. Bot. 60:3715-3726(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, TISSUE SPECIFICITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY082343 mRNA. Translation: AAL92879.1.

3D structure databases

ProteinModelPortalQ8RVK9.
SMRQ8RVK9. Positions 3-389.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-12039.

Family and domain databases

Gene3D3.40.47.10. 2 hits.
InterProIPR012328. Chalcone/stilbene_synth_C.
IPR018088. Chalcone/stilbene_synthase_AS.
IPR001099. Chalcone/stilbene_synthase_N.
IPR011141. Polyketide_synthase_type-III.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamPF02797. Chal_sti_synt_C. 1 hit.
PF00195. Chal_sti_synt_N. 1 hit.
[Graphical view]
PIRSFPIRSF000451. PKS_III. 1 hit.
SUPFAMSSF53901. SSF53901. 2 hits.
PROSITEPS00441. CHALCONE_SYNTH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCHS_CANSA
AccessionPrimary (citable) accession number: Q8RVK9
Entry history
Integrated into UniProtKB/Swiss-Prot: February 6, 2013
Last sequence update: June 1, 2002
Last modified: February 19, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families