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Q8RVK9

- CHS_CANSA

UniProt

Q8RVK9 - CHS_CANSA

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Protein
Naringenin-chalcone synthase
Gene
CHS, CAN1069
Organism
Cannabis sativa (Hemp) (Marijuana)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Chalcone synthase that can also use isovaleryl-CoA, isobutyryl-CoA or hexanoyl-CoA as substrates, but that is unable produce olivetol or olivetolic acid.

Catalytic activityi

3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei164 – 1641 By similarity

GO - Molecular functioni

  1. naringenin-chalcone synthase activity Source: UniProtKB

GO - Biological processi

  1. cannabinoid biosynthetic process Source: UniProtKB
  2. chalcone biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-12039.

Names & Taxonomyi

Protein namesi
Recommended name:
Naringenin-chalcone synthase (EC:2.3.1.74)
Gene namesi
Name:CHS
Synonyms:CAN1069
OrganismiCannabis sativa (Hemp) (Marijuana)
Taxonomic identifieri3483 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsRosalesCannabaceaeCannabis

Subcellular locationi

Cytoplasm Inferred

GO - Cellular componenti

  1. polyketide synthase complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 389389Naringenin-chalcone synthase
PRO_0000421153Add
BLAST

Expressioni

Tissue specificityi

Expressed in glandular trichomes. Detected at low levels in female flowers, stems, seeds, leaves and roots.2 Publications

Structurei

3D structure databases

ProteinModelPortaliQ8RVK9.
SMRiQ8RVK9. Positions 3-389.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.47.10. 2 hits.
InterProiIPR012328. Chalcone/stilbene_synth_C.
IPR018088. Chalcone/stilbene_synthase_AS.
IPR001099. Chalcone/stilbene_synthase_N.
IPR011141. Polyketide_synthase_type-III.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamiPF02797. Chal_sti_synt_C. 1 hit.
PF00195. Chal_sti_synt_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000451. PKS_III. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
PROSITEiPS00441. CHALCONE_SYNTH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8RVK9-1 [UniParc]FASTAAdd to Basket

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MVTVEEFRKA QRAEGPATIM AIGTATPANC VLQSEYPDYY FRITNSEHKT    50
ELKEKFKRMC DKSMIRKRYM HLTEEILKEN PNLCAYEAPS LDARQDMVVV 100
EVPKLGKEAA TKAIKEWGQP KSKITHLVFC TTSGVDMPGA DYQLTKLLGL 150
RPSVKRLMMY QQGCFAGGTV LRLAKDLAEN NKGARVLVVC SEITAVTFRG 200
PNDTHLDSLV GQALFGDGSA ALIVGSDPIP EVEKPIFELV SAAQTILPDS 250
DGAIDGHLRE VGLTFHLLKD VPGLISKNIE KSLNEAFKPL GISDWNSLFW 300
IAHPGGPAIL DQVESKLALK TEKLRATRHV LSEYGNMSSA CVLFILDEMR 350
RKCVEDGLNT TGEGLEWGVL FGFGPGLTVE TVVLHSVAI 389
Length:389
Mass (Da):42,720
Last modified:June 1, 2002 - v1
Checksum:i51C5C0C87EFF6279
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY082343 mRNA. Translation: AAL92879.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY082343 mRNA. Translation: AAL92879.1 .

3D structure databases

ProteinModelPortali Q8RVK9.
SMRi Q8RVK9. Positions 3-389.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-12039.

Family and domain databases

Gene3Di 3.40.47.10. 2 hits.
InterProi IPR012328. Chalcone/stilbene_synth_C.
IPR018088. Chalcone/stilbene_synthase_AS.
IPR001099. Chalcone/stilbene_synthase_N.
IPR011141. Polyketide_synthase_type-III.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view ]
Pfami PF02797. Chal_sti_synt_C. 1 hit.
PF00195. Chal_sti_synt_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000451. PKS_III. 1 hit.
SUPFAMi SSF53901. SSF53901. 2 hits.
PROSITEi PS00441. CHALCONE_SYNTH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and over-expression of a cDNA encoding a polyketide synthase from Cannabis sativa."
    Raharjo T.J., Chang W.T., Verberne M.C., Peltenburg-Looman A.M., Linthorst H.J., Verpoorte R.
    Plant Physiol. Biochem. 42:291-297(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, TISSUE SPECIFICITY.
    Strain: cv. Four-way.
    Tissue: Leaf.
  2. "Identification of candidate genes affecting Delta9-tetrahydrocannabinol biosynthesis in Cannabis sativa."
    Marks M.D., Tian L., Wenger J.P., Omburo S.N., Soto-Fuentes W., He J., Gang D.R., Weiblen G.D., Dixon R.A.
    J. Exp. Bot. 60:3715-3726(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, TISSUE SPECIFICITY.

Entry informationi

Entry nameiCHS_CANSA
AccessioniPrimary (citable) accession number: Q8RVK9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 6, 2013
Last sequence update: June 1, 2002
Last modified: February 19, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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