Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q8RVK9

- CHS_CANSA

UniProt

Q8RVK9 - CHS_CANSA

Protein

Naringenin-chalcone synthase

Gene

CHS

Organism
Cannabis sativa (Hemp) (Marijuana)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 59 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Chalcone synthase that can also use isovaleryl-CoA, isobutyryl-CoA or hexanoyl-CoA as substrates, but that is unable produce olivetol or olivetolic acid.

    Catalytic activityi

    3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2.2 PublicationsPROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei164 – 1641PROSITE-ProRule annotation

    GO - Molecular functioni

    1. naringenin-chalcone synthase activity Source: UniProtKB

    GO - Biological processi

    1. cannabinoid biosynthetic process Source: UniProtKB
    2. chalcone biosynthetic process Source: UniProtKB

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-12039.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Naringenin-chalcone synthase (EC:2.3.1.74)
    Gene namesi
    Name:CHS
    Synonyms:CAN1069
    OrganismiCannabis sativa (Hemp) (Marijuana)
    Taxonomic identifieri3483 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsRosalesCannabaceaeCannabis

    Subcellular locationi

    Cytoplasm Curated

    GO - Cellular componenti

    1. polyketide synthase complex Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 389389Naringenin-chalcone synthasePRO_0000421153Add
    BLAST

    Expressioni

    Tissue specificityi

    Expressed in glandular trichomes. Detected at low levels in female flowers, stems, seeds, leaves and roots.2 Publications

    Structurei

    3D structure databases

    ProteinModelPortaliQ8RVK9.
    SMRiQ8RVK9. Positions 3-389.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the chalcone/stilbene synthases family.Curated

    Family and domain databases

    Gene3Di3.40.47.10. 2 hits.
    InterProiIPR012328. Chalcone/stilbene_synth_C.
    IPR018088. Chalcone/stilbene_synthase_AS.
    IPR001099. Chalcone/stilbene_synthase_N.
    IPR011141. Polyketide_synthase_type-III.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    [Graphical view]
    PfamiPF02797. Chal_sti_synt_C. 1 hit.
    PF00195. Chal_sti_synt_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000451. PKS_III. 1 hit.
    SUPFAMiSSF53901. SSF53901. 2 hits.
    PROSITEiPS00441. CHALCONE_SYNTH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8RVK9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVTVEEFRKA QRAEGPATIM AIGTATPANC VLQSEYPDYY FRITNSEHKT    50
    ELKEKFKRMC DKSMIRKRYM HLTEEILKEN PNLCAYEAPS LDARQDMVVV 100
    EVPKLGKEAA TKAIKEWGQP KSKITHLVFC TTSGVDMPGA DYQLTKLLGL 150
    RPSVKRLMMY QQGCFAGGTV LRLAKDLAEN NKGARVLVVC SEITAVTFRG 200
    PNDTHLDSLV GQALFGDGSA ALIVGSDPIP EVEKPIFELV SAAQTILPDS 250
    DGAIDGHLRE VGLTFHLLKD VPGLISKNIE KSLNEAFKPL GISDWNSLFW 300
    IAHPGGPAIL DQVESKLALK TEKLRATRHV LSEYGNMSSA CVLFILDEMR 350
    RKCVEDGLNT TGEGLEWGVL FGFGPGLTVE TVVLHSVAI 389
    Length:389
    Mass (Da):42,720
    Last modified:June 1, 2002 - v1
    Checksum:i51C5C0C87EFF6279
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY082343 mRNA. Translation: AAL92879.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY082343 mRNA. Translation: AAL92879.1 .

    3D structure databases

    ProteinModelPortali Q8RVK9.
    SMRi Q8RVK9. Positions 3-389.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-12039.

    Family and domain databases

    Gene3Di 3.40.47.10. 2 hits.
    InterProi IPR012328. Chalcone/stilbene_synth_C.
    IPR018088. Chalcone/stilbene_synthase_AS.
    IPR001099. Chalcone/stilbene_synthase_N.
    IPR011141. Polyketide_synthase_type-III.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    [Graphical view ]
    Pfami PF02797. Chal_sti_synt_C. 1 hit.
    PF00195. Chal_sti_synt_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000451. PKS_III. 1 hit.
    SUPFAMi SSF53901. SSF53901. 2 hits.
    PROSITEi PS00441. CHALCONE_SYNTH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and over-expression of a cDNA encoding a polyketide synthase from Cannabis sativa."
      Raharjo T.J., Chang W.T., Verberne M.C., Peltenburg-Looman A.M., Linthorst H.J., Verpoorte R.
      Plant Physiol. Biochem. 42:291-297(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, TISSUE SPECIFICITY.
      Strain: cv. Four-way.
      Tissue: Leaf.
    2. "Identification of candidate genes affecting Delta9-tetrahydrocannabinol biosynthesis in Cannabis sativa."
      Marks M.D., Tian L., Wenger J.P., Omburo S.N., Soto-Fuentes W., He J., Gang D.R., Weiblen G.D., Dixon R.A.
      J. Exp. Bot. 60:3715-3726(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiCHS_CANSA
    AccessioniPrimary (citable) accession number: Q8RVK9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 6, 2013
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 59 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3