Q8RVK9 (CHS_CANSA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Naringenin-chalcone synthase
EC=2.3.1.74

Gene names

Name:	CHS
Synonyms:	CAN1069

Organism

Cannabis sativa (Hemp) (Marijuana)

Taxonomic identifier

3483 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Rosales › Cannabaceae › Cannabis

Protein attributes

Sequence length	389 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Chalcone synthase that can also use isovaleryl-CoA, isobutyryl-CoA or hexanoyl-CoA as substrates, but that is unable produce olivetol or olivetolic acid.
Catalytic activity	3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2. Ref.1 Ref.2
Subcellular location	Cytoplasm Probable.
Tissue specificity	Expressed in glandular trichomes. Detected at low levels in female flowers, stems, seeds, leaves and roots. Ref.1 Ref.2
Sequence similarities	Belongs to the chalcone/stilbene synthases family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological_process	cannabinoid biosynthetic process Non-traceable author statement Ref.1. Source: UniProtKB chalcone biosynthetic process Inferred from direct assay Ref.1. Source: UniProtKB
Cellular_component	polyketide synthase complex Inferred from direct assay Ref.1. Source: UniProtKB
Molecular_function	naringenin-chalcone synthase activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 389	389	Naringenin-chalcone synthase		PRO_0000421153
Sites
	Active site	164	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8RVK9 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 51C5C0C87EFF6279	FASTA	389	42,720
	10 20 30 40 50 60 MVTVEEFRKA QRAEGPATIM AIGTATPANC VLQSEYPDYY FRITNSEHKT ELKEKFKRMC 70 80 90 100 110 120 DKSMIRKRYM HLTEEILKEN PNLCAYEAPS LDARQDMVVV EVPKLGKEAA TKAIKEWGQP 130 140 150 160 170 180 KSKITHLVFC TTSGVDMPGA DYQLTKLLGL RPSVKRLMMY QQGCFAGGTV LRLAKDLAEN 190 200 210 220 230 240 NKGARVLVVC SEITAVTFRG PNDTHLDSLV GQALFGDGSA ALIVGSDPIP EVEKPIFELV 250 260 270 280 290 300 SAAQTILPDS DGAIDGHLRE VGLTFHLLKD VPGLISKNIE KSLNEAFKPL GISDWNSLFW 310 320 330 340 350 360 IAHPGGPAIL DQVESKLALK TEKLRATRHV LSEYGNMSSA CVLFILDEMR RKCVEDGLNT 370 380 TGEGLEWGVL FGFGPGLTVE TVVLHSVAI

References

[1]	"Cloning and over-expression of a cDNA encoding a polyketide synthase from Cannabis sativa." Raharjo T.J., Chang W.T., Verberne M.C., Peltenburg-Looman A.M., Linthorst H.J., Verpoorte R. Plant Physiol. Biochem. 42:291-297(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, TISSUE SPECIFICITY. Strain: cv. Four-way. Tissue: Leaf.
[2]	"Identification of candidate genes affecting Delta9-tetrahydrocannabinol biosynthesis in Cannabis sativa." Marks M.D., Tian L., Wenger J.P., Omburo S.N., Soto-Fuentes W., He J., Gang D.R., Weiblen G.D., Dixon R.A. J. Exp. Bot. 60:3715-3726(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY082343 mRNA. Translation: AAL92879.1.
3D structure databases
HSSP	HSSP built from PDB template 1BI5 based on UniProtKB P30074.
ProteinModelPortal	Q8RVK9.
SMR	Q8RVK9. Positions 3-389.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-12039.
Family and domain databases
Gene3D	3.40.47.10. 2 hits.
InterPro	IPR012328. Chalcone/stilbene_synth_C. IPR018088. Chalcone/stilbene_synthase_AS. IPR001099. Chalcone/stilbene_synthase_N. IPR011141. Polyketide_synthase_type-III. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. [Graphical view]
Pfam	PF02797. Chal_sti_synt_C. 1 hit. PF00195. Chal_sti_synt_N. 1 hit. [Graphical view]
PIRSF	PIRSF000451. PKS_III. 1 hit.
SUPFAM	SSF53901. Thiolase-like. 2 hits.
PROSITE	PS00441. CHALCONE_SYNTH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHS_CANSA

Accession

Primary (citable) accession number: Q8RVK9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 6, 2013
Last sequence update:	June 1, 2002
Last modified:	April 3, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program