Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Serine carboxypeptidase-like 8

Gene

SCPL8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in plants secondary metabolism. Functions as acyltransferase to form the sinapate ester sinapoylmalate. Also capable of catalyzing the formation of 1,2-bis-O-sinapoyl beta-D-glucoside.5 Publications

Catalytic activityi

1-O-sinapoyl-beta-D-glucose + (S)-malate = D-glucose + sinapoyl-(S)-malate.
2 1-O-sinapoyl beta-D-glucoside = D-glucose + 1,2-bis-O-sinapoyl beta-D-glucoside.

Enzyme regulationi

95% inhibition by diisopropyl fluorophosphate (DFP) and 30% by phenylmethylsulfonyl fluoride (PMSF).2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei173By similarity1
Active sitei358By similarity1
Active sitei411By similarity1

GO - Molecular functioni

GO - Biological processi

  • phenylpropanoid metabolic process Source: TAIR
  • proteolysis Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

BioCyciARA:AT2G22990-MONOMER.
BRENDAi2.3.1.92. 399.

Protein family/group databases

ESTHERiarath-SCP8. Carboxypeptidase_S10.
MEROPSiS10.A14.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine carboxypeptidase-like 8
Alternative name(s):
Protein SINAPOYLGLUCOSE ACCUMULATOR 1
Sinapoylglucose--malate O-sinapoyltransferase (EC:2.3.1.92)
Short name:
SMT
Sinapoylglucose--sinapoylglucose O-sinapoyltransferase (EC:2.3.1.103)
Gene namesi
Name:SCPL8
Synonyms:SMT, SNG1
Ordered Locus Names:At2g22990
ORF Names:F21P24.5, T20K9.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G22990.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Vacuole

Pathology & Biotechi

Disruption phenotypei

Plants do not contain sinapoylmalate and accumulate its biosynthetic precursor, sinapoylglucose.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi73N → A: 87% reduction of activity. 1 Publication1
Mutagenesisi172 – 175DSYS → ESYA: 85% reduction of activity. 1 Publication4
Mutagenesisi173S → A: Total loss of activity. 1 Publication1
Mutagenesisi268K → E: 25% reduction of activity. 1 Publication1
Mutagenesisi272H → D: 78% reduction of activity. 1 Publication1
Mutagenesisi322R → E: 99% reduction of activity. 1 Publication1
Mutagenesisi358D → A: 80% reduction of activity. 1 Publication1
Mutagenesisi411H → A: Total loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 192 PublicationsAdd BLAST19
ChainiPRO_000027462220 – 433Serine carboxypeptidase-like 8Add BLAST414

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi78 ↔ 323By similarity
Glycosylationi99N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi241 ↔ 255By similarity
Disulfide bondi279 ↔ 289By similarity
Glycosylationi283N-linked (GlcNAc...)Sequence analysis1
Glycosylationi324N-linked (GlcNAc...)Sequence analysis1
Glycosylationi342N-linked (GlcNAc...)Sequence analysis1
Glycosylationi418N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8RUW5.

Expressioni

Tissue specificityi

Highly expressed in seedlings. Expressed in leaves, stems, flowers and siliques, and at low levels in roots.3 Publications

Gene expression databases

ExpressionAtlasiQ8RUW5. baseline and differential.
GenevisibleiQ8RUW5. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G22990.3.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DRFmodel-B20-433[»]
ProteinModelPortaliQ8RUW5.
SMRiQ8RUW5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S10 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1282. Eukaryota.
COG2939. LUCA.
HOGENOMiHOG000198297.
InParanoidiQ8RUW5.
KOiK09757.
OMAiECWANDE.
PhylomeDBiQ8RUW5.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR001563. Peptidase_S10.
[Graphical view]
PANTHERiPTHR11802. PTHR11802. 1 hit.
PfamiPF00450. Peptidase_S10. 1 hit.
[Graphical view]
PRINTSiPR00724. CRBOXYPTASEC.
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8RUW5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLKIKFLLL LVLYHHVDSA SIVKFLPGFE GPLPFELETG YIGIGEDENV
60 70 80 90 100
QFFYYFIKSE NNPKEDPLLI WLNGGPGCSC LGGIIFENGP VGLKFEVFNG
110 120 130 140 150
SAPSLFSTTY SWTKMANIIF LDQPVGSGFS YSKTPIDKTG DISEVKRTHE
160 170 180 190 200
FLQKWLSRHP QYFSNPLYVV GDSYSGMIVP ALVQEISQGN YICCEPPINL
210 220 230 240 250
QGYMLGNPVT YMDFEQNFRI PYAYGMGLIS DEIYEPMKRI CNGNYYNVDP
260 270 280 290 300
SNTQCLKLTE EYHKCTAKIN IHHILTPDCD VTNVTSPDCY YYPYHLIECW
310 320 330 340 350
ANDESVREAL HIEKGSKGKW ARCNRTIPYN HDIVSSIPYH MNNSISGYRS
360 370 380 390 400
LIYSGDHDIA VPFLATQAWI RSLNYSPIHN WRPWMINNQI AGYTRAYSNK
410 420 430
MTFATIKGGG HTAEYRPNET FIMFQRWISG QPL
Length:433
Mass (Da):49,439
Last modified:February 6, 2007 - v2
Checksum:i67234085A5FBAF0C
GO
Isoform 2 (identifier: Q8RUW5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     408-433: GGGHTAEYRPNETFIMFQRWISGQPL → ASVDTRQSID...NIHVKRSCLC

Note: Derived from EST data. May be due to a competing donor splice site. No experimental confirmation available.
Show »
Length:458
Mass (Da):52,122
Checksum:iDC1C8BB0FF1E94E8
GO

Sequence cautioni

The sequence AAC17816 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAK32769 differs from that shown. Reason: Frameshift at position 260.Curated
The sequence AAM15006 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAN28819 differs from that shown. Reason: Erroneous termination at position 260. Translated as Glu.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_027464408 – 433GGGHT…SGQPL → ASVDTRQSIDQTRPLSCSKG GSVANPCNKRLMTFTYNYLP TNIHVKRSCLC in isoform 2. CuratedAdd BLAST26

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF275313 mRNA. Translation: AAF78760.1.
AC004401 Genomic DNA. Translation: AAC17816.2. Sequence problems.
AC004786 Genomic DNA. Translation: AAM15006.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC07390.1.
CP002685 Genomic DNA. Translation: AEC07393.1.
AF361601 mRNA. Translation: AAK32769.1. Frameshift.
AY035052 mRNA. Translation: AAK59557.1.
AY051060 mRNA. Translation: AAK93737.1.
AY143880 mRNA. Translation: AAN28819.1. Sequence problems.
PIRiC84619.
RefSeqiNP_850034.1. NM_179703.2. [Q8RUW5-1]
NP_850035.1. NM_179704.1. [Q8RUW5-2]
UniGeneiAt.24365.

Genome annotation databases

EnsemblPlantsiAT2G22990.1; AT2G22990.1; AT2G22990. [Q8RUW5-1]
GeneIDi816830.
GrameneiAT2G22990.1; AT2G22990.1; AT2G22990.
KEGGiath:AT2G22990.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF275313 mRNA. Translation: AAF78760.1.
AC004401 Genomic DNA. Translation: AAC17816.2. Sequence problems.
AC004786 Genomic DNA. Translation: AAM15006.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC07390.1.
CP002685 Genomic DNA. Translation: AEC07393.1.
AF361601 mRNA. Translation: AAK32769.1. Frameshift.
AY035052 mRNA. Translation: AAK59557.1.
AY051060 mRNA. Translation: AAK93737.1.
AY143880 mRNA. Translation: AAN28819.1. Sequence problems.
PIRiC84619.
RefSeqiNP_850034.1. NM_179703.2. [Q8RUW5-1]
NP_850035.1. NM_179704.1. [Q8RUW5-2]
UniGeneiAt.24365.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DRFmodel-B20-433[»]
ProteinModelPortaliQ8RUW5.
SMRiQ8RUW5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G22990.3.

Protein family/group databases

ESTHERiarath-SCP8. Carboxypeptidase_S10.
MEROPSiS10.A14.

Proteomic databases

PaxDbiQ8RUW5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G22990.1; AT2G22990.1; AT2G22990. [Q8RUW5-1]
GeneIDi816830.
GrameneiAT2G22990.1; AT2G22990.1; AT2G22990.
KEGGiath:AT2G22990.

Organism-specific databases

TAIRiAT2G22990.

Phylogenomic databases

eggNOGiKOG1282. Eukaryota.
COG2939. LUCA.
HOGENOMiHOG000198297.
InParanoidiQ8RUW5.
KOiK09757.
OMAiECWANDE.
PhylomeDBiQ8RUW5.

Enzyme and pathway databases

BioCyciARA:AT2G22990-MONOMER.
BRENDAi2.3.1.92. 399.

Miscellaneous databases

PROiQ8RUW5.

Gene expression databases

ExpressionAtlasiQ8RUW5. baseline and differential.
GenevisibleiQ8RUW5. AT.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR001563. Peptidase_S10.
[Graphical view]
PANTHERiPTHR11802. PTHR11802. 1 hit.
PfamiPF00450. Peptidase_S10. 1 hit.
[Graphical view]
PRINTSiPR00724. CRBOXYPTASEC.
SUPFAMiSSF53474. SSF53474. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSCP8_ARATH
AccessioniPrimary (citable) accession number: Q8RUW5
Secondary accession number(s): O64809, Q3EBV9, Q9ASY4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: February 6, 2007
Last modified: November 30, 2016
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

In cv. Pna-10, this protein SCP8 and the adjacent SCP10 are not present due to a natural 13-kb deletion (PubMed:19969522).1 Publication

Caution

Was classified as a serine carboxypeptidase-like (SCPL) protein solely on the basis of the overall sequence similarity (PubMed:15908604) but it has been shown that it belongs to a class of enzymes that catalyze acyltransferase reactions (PubMed:17600138).2 Publications

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.