Q8RUV9 (BGAL1_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-galactosidase 1
Short name=Lactase 1
EC=3.2.1.23

Gene names

Ordered Locus Names:	Os01g0533400, LOC_Os01g34920
ORF Names:	OJ1029_F04.5, OJ1619_F12.26

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	827 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.
Subcellular location	Secreted › extracellular space › apoplast Potential.
Sequence similarities	Belongs to the glycosyl hydrolase 35 family. Contains 1 SUEL-type lectin domain.

Ontologies

Keywords
Cellular component	Apoplast Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-galactosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro sugar binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 827	802	Beta-galactosidase 1		PRO_0000294152
Regions
	Domain	746 – 827	82	SUEL-type lectin
Sites
	Active site	187	1	Proton donor Potential
	Active site	259	1	Nucleophile Potential
Amino acid modifications
	Glycosylation	198	1	N-linked (GlcNAc...) Potential
	Glycosylation	249	1	N-linked (GlcNAc...) Potential
	Glycosylation	260	1	N-linked (GlcNAc...) Potential
	Glycosylation	366	1	N-linked (GlcNAc...) Potential
	Glycosylation	392	1	N-linked (GlcNAc...) Potential
	Glycosylation	502	1	N-linked (GlcNAc...) Potential
	Glycosylation	520	1	N-linked (GlcNAc...) Potential
	Glycosylation	578	1	N-linked (GlcNAc...) Potential
	Glycosylation	586	1	N-linked (GlcNAc...) Potential
	Glycosylation	615	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8RUV9 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 749E7C4D35FEA80B	FASTA	827	91,711
	10 20 30 40 50 60 MMGRRGSSWC RWWVALLVLA VAADAVGCTS VSYDDRSLVI DGQRRIILSG SIHYPRSTPE 70 80 90 100 110 120 MWPDLIKKAK EGGLDAIETY IFWNGHEPHR RQYNFEGNYD VVRFFKEIQN AGMYAILRIG 130 140 150 160 170 180 PYICGEWNYG GLPAWLRDIP GMQFRLHNEP FENEMETFTT LIVNKMKDSK MFAEQGGPII 190 200 210 220 230 240 LAQIENEYGN IMGKLNNNQS ASEYIHWCAD MANKQNVGVP WIMCQQDDDV PHNVVNTCNG 250 260 270 280 290 300 FYCHDWFPNR TGIPKIWTEN WTGWFKAWDK PDFHRSAEDI AFAVAMFFQK RGSLQNYYMY 310 320 330 340 350 360 HGGTNFGRTS GGPYITTSYD YDAPLDEYGN LRQPKYGHLK ELHSVLKSME KTLVHGEYFD 370 380 390 400 410 420 TNYGDNITVT KYTLDSSSAC FINNRFDDKD VNVTLDGATH LLPAWSVSIL PDCKTVAFNS 430 440 450 460 470 480 AKIKTQTSVM VKKPNTAEQE QESLKWSWMP ENLSPFMTDE KGNFRKNELL EQIVTSTDQS 490 500 510 520 530 540 DYLWYRTSLN HKGEGSYKLY VNTTGHELYA FVNGKLIGKN HSADGDFVFQ LESPVKLHDG 550 560 570 580 590 600 KNYISLLSAT VGLKNYGPSF EKMPTGIVGG PVKLIDSNGT AIDLSNSSWS YKAGLASEYR 610 620 630 640 650 660 QIHLDKPGYK WNGNNGTIPI NRPFTWYKAT FEAPSGEDAV VVDLLGLNKG VAWVNGNNLG 670 680 690 700 710 720 RYWPSYTAAE MAGCHRCDYR GAFQAEGDGT RCLTGCGEPS QRYYHVPRSF LAAGEPNTLL 730 740 750 760 770 780 LFEEAGGDPS GVALRTVVPG AVCTSGEAGD AVTLSCGGGH AVSSVDVASF GVGRGRCGGY 790 800 810 820 EGGCESKAAY EAFTAACVGK ESCTVEITGA FAGAGCLSGV LTVQATC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed: 18089549] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP003445 Genomic DNA. Translation: BAB89138.1. AP003447 Genomic DNA. Translation: BAB90329.1. AP008207 Genomic DNA. Translation: BAF05166.1. AK119447 mRNA. No translation available.
RefSeq	NP_001043252.1. NM_001049787.1.
UniGene	Os.41043.
3D structure databases
ProteinModelPortal	Q8RUV9.
ModBase	Search...
Protein family/group databases
CAZy	GH35. Glycoside Hydrolase Family 35.
Proteomic databases
PRIDE	Q8RUV9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4327808.
KEGG	osa:4327808.
Organism-specific databases
Gramene	Q8RUV9.
Phylogenomic databases
HOGENOM	HBG318019.
OMA	TSVMVKK.
PhylomeDB	Q8RUV9.
ProtClustDB	CLSN2691488.
Family and domain databases
InterPro	IPR008979. Galactose-bd-like. IPR019801. Glyco_hydro_35_CS. IPR013781. Glyco_hydro_subgr_catalytic. IPR001944. Glycoside_Hdrlase_35. IPR017853. Glycoside_hydrolase_SF. IPR000922. Lectin_gal-bd_dom. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR23421. Glyco_hydro_35. 1 hit.
Pfam	PF02140. Gal_Lectin. 1 hit. PF01301. Glyco_hydro_35. 1 hit. [Graphical view]
PRINTS	PR00742. GLHYDRLASE35.
SUPFAM	SSF49785. Gal_bind_like. 2 hits. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS01182. GLYCOSYL_HYDROL_F35. 1 hit. PS50228. SUEL_LECTIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGAL1_ORYSJ

Accession

Primary (citable) accession number: Q8RUV9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	June 1, 2002
Last modified:	December 14, 2011
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program