Q8RUN1 (ACA3_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Calcium-transporting ATPase 3, plasma membrane-type
EC=3.6.3.8

Alternative name(s):
Ca(2+)-ATPase isoform 3

Gene names

Ordered Locus Names:	Os01g0939100, LOC_Os01g71240
ORF Names:	B1150F11.11, P0504E02.35

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	1043 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol out of the cell, into the endoplasmic reticulum, or into organelles By similarity.
Catalytic activity	ATP + H2O + Ca2+[side 1] = ADP + phosphate + Ca2+[side 2].
Enzyme regulation	Activated by calmodulin By similarity.
Subcellular location	Membrane; Multi-pass membrane protein By similarity.
Domain	The N-terminus contains an autoinhibitory calmodulin-binding domain, which binds calmodulin in a calcium-dependent fashion By similarity.
Sequence similarities	Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily. [View classification]

Ontologies

Keywords
Biological process	Calcium transport Ion transport Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Calcium Calmodulin-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	ATP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calcium-transporting ATPase activity Inferred from electronic annotation. Source: EC calmodulin binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1043	1043	Calcium-transporting ATPase 3, plasma membrane-type		PRO_0000247302
Regions
	Topological domain	1 – 178	178	Cytoplasmic Potential
	Transmembrane	179 – 199	21	Helical; Potential
	Transmembrane	202 – 222	21	Helical; Potential
	Topological domain	223 – 258	36	Cytoplasmic Potential
	Transmembrane	259 – 279	21	Helical; Potential
	Transmembrane	356 – 376	21	Helical; Potential
	Topological domain	377 – 395	19	Cytoplasmic Potential
	Transmembrane	396 – 416	21	Helical; Potential
	Transmembrane	824 – 844	21	Helical; Potential
	Topological domain	845	1	Cytoplasmic Potential
	Transmembrane	846 – 866	21	Helical; Potential
	Transmembrane	891 – 911	21	Helical; Potential
	Topological domain	912 – 955	44	Cytoplasmic Potential
	Transmembrane	956 – 976	21	Helical; Potential
	Transmembrane	998 – 1018	21	Helical; Potential
	Topological domain	1019 – 1043	25	Cytoplasmic Potential
Sites
	Active site	460	1	4-aspartylphosphate intermediate By similarity
	Metal binding	761	1	Magnesium By similarity
	Metal binding	765	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8RUN1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: A16B82561803AF56	FASTA	1,043	112,842
	10 20 30 40 50 60 MSFIRKKSME FLKSFEVPAK NPSEEAQRRW RDAVGTLVKN RRRRFRMVPD LDKRSQAETQ 70 80 90 100 110 120 RRKIQEKLRV ALFVQKAALQ FIDAVRKTEH PLPELARQCG FSVSAEELAS IVRGHDTKSL 130 140 150 160 170 180 RFHNGVDGIA RKVAVSLADG VKSDDAGLRA EVYGANQYTE KPPRTFWMFL WDASQDMTLL 190 200 210 220 230 240 LLAFCAAVSV AIGLATEGWP SGMYDGVGIM LTILLVVMIT AASDYKQSLQ FRDLDKEKKK 250 260 270 280 290 300 IDVQVTRDGY RQKVSIYDIV VGDIVHLSIG DQVPADGLFI DGYSFVVDES NLSGESEPVH 310 320 330 340 350 360 VSTANRFLLG GTKVQDGSAR MLVTAVGMRT EWGNLMETLS QGGEDETPLQ VKLNGVATII 370 380 390 400 410 420 GKIGLAFAVL TFTVLMARFL LGKAGAPGGL LRWRMVDALA VLNFFAVAVT IIVVAVPEGL 430 440 450 460 470 480 PLAVTLSLAF AMKKLMQERA LVRHLSACET MGSASCICTD KTGTLTTNHM VVEKIWASGA 490 500 510 520 530 540 AQTMSNAKGF DQLTSSMSET FAKVLLEGVF HCSGSEVVRG KDGRHTIMGT PTETAILEFG 550 560 570 580 590 600 LAVEKRARIE HTGAGKLKVE PFNSVKKTMA VVIASPSAGG RPRAFLKGAS EVVLSRCSLV 610 620 630 640 650 660 LDGTGNVEKL TDAKAKRVAS AIDAFACEAL RTLCLAYQDV DGGGGDIPGE GYTLIAVFGI 670 680 690 700 710 720 KDPLRPGVRE AVATCHAAGI NVRMVTGDNI NTAKAIAREC GILTDDGIAI EGPEFRNKDP 730 740 750 760 770 780 DQMREIIPKI QVMARSLPLD KHTLVTNLRG MFNEVVAVTG DGTNDAPALH EADIGLAMGI 790 800 810 820 830 840 AGTEVAKENA DVIIMDDNFS TIINVAKWGR SVYINIQKFV QFQLTVNVVA LMVNFISASF 850 860 870 880 890 900 TGSAPLTIVQ LLWVNLIMDT LGALALATEP PNDAMMKRPP VGRGDNFITK VMWRNIVGQS 910 920 930 940 950 960 IYQLVVLGVL LLRGKSLLQI NGPQADSLLN TFVFNTFVFC QVFNEVNSRE MEKINVFSGI 970 980 990 1000 1010 1020 FSSWIFSAVV GVTAGFQVIM VELLGTFANT VHLSGKLWLT SVLIGSVGLV IGAILKCIPV 1030 1040 ESGSDASDRH DGYRPIPTGP SAV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed: 18089549] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP003269 Genomic DNA. Translation: BAB89725.1. AP003412 Genomic DNA. Translation: BAB90248.1. AP008207 Genomic DNA. Translation: BAF07259.1. AK070064 mRNA. Translation: BAG91749.1.
RefSeq	NP_001045345.1. NM_001051880.1.
UniGene	Os.34808.
3D structure databases
ProteinModelPortal	Q8RUN1.
ModBase	Search...
Proteomic databases
PRIDE	Q8RUN1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4326507.
KEGG	osa:4326507.
NMPDR	fig|39947.1.peg.7045.
Organism-specific databases
Gramene	Q8RUN1.
Phylogenomic databases
HOGENOM	HBG456486.
OMA	HIESEIV.
PhylomeDB	Q8RUN1.
ProtClustDB	CLSN2692275.
Family and domain databases
InterPro	IPR008250. ATPase_P-typ_ATPase-assoc-dom. IPR006408. ATPase_P-typ_Ca-transp_PMCA. IPR006068. ATPase_P-typ_cation-transptr_C. IPR004014. ATPase_P-typ_cation-transptr_N. IPR023300. ATPase_P-typ_cyto_domA. IPR023299. ATPase_P-typ_cyto_domN. IPR000695. ATPase_P-typ_H-transp. IPR001757. ATPase_P-typ_ion-transptr. IPR018303. ATPase_P-typ_P_site. IPR023298. ATPase_P-typ_TM_dom. IPR024750. Ca_ATPase_N_dom. IPR005834. Dehalogen-like_hydro. IPR023214. HAD-like_dom. [Graphical view]
Gene3D	G3DSA:2.70.150.10. ATPase_P-typ_cyto_domA. 2 hits. G3DSA:3.40.1110.10. ATPase_P-typ_cyto_domN. 1 hit. G3DSA:1.20.1110.10. ATPase_P-typ_TM_dom. 1 hit.
Pfam	PF12515. CaATP_NAI. 1 hit. PF00689. Cation_ATPase_C. 1 hit. PF00690. Cation_ATPase_N. 1 hit. PF00122. E1-E2_ATPase. 1 hit. PF00702. Hydrolase. 1 hit. [Graphical view]
PRINTS	PR00119. CATATPASE. PR00120. HATPASE.
SMART	SM00831. Cation_ATPase_N. 1 hit. [Graphical view]
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01517. ATPase-IIB_Ca. 1 hit. TIGR01494. ATPase_P-type. 4 hits.
PROSITE	PS00154. ATPASE_E1_E2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACA3_ORYSJ

Accession

Primary (citable) accession number: Q8RUN1
Secondary accession number(s): Q0JG69

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	June 1, 2002
Last modified:	January 25, 2012
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program