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Protein

Ubiquitin-NEDD8-like protein RUB2

Gene

RUB2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, and DNA-damage responses. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling (By similarity).By similarity
NEDD8-like protein RUB2: Appears to function as a stable post-translational protein modifier.

Miscellaneous

Ubiquitin is encoded by 16 different genes. Ubiquitin is generally synthesized as a polyubiquitin precursor with tandem head to tail repeats. Often, there is one to three additional amino acids after the last repeat, removed in the mature protein. Alternatively, ubiquitin extension protein is synthesized as a single copy of ubiquitin fused to a ribosomal protein (either L40 or S27A) or to a ubiquitin-related protein (either RUB1 or RUB2). Following translation, extension protein is cleaved from ubiquitin.

GO - Biological processi

  • ubiquitin-dependent protein catabolic process Source: TAIR

Keywordsi

Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-ATH-5689603. UCH proteinases.
R-ATH-8951664. Neddylation.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-NEDD8-like protein RUB2
Cleaved into the following 2 chains:
Alternative name(s):
Ubiquitin-related protein 2
Short name:
AtRUB2
Gene namesi
Name:RUB2
Synonyms:UBQ7
Ordered Locus Names:At2g35635
ORF Names:T20F21.24
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G35635.
TAIRilocus:505006295. AT2G35635.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003969111 – 76UbiquitinAdd BLAST76
ChainiPRO_000003596777 – 152NEDD8-like protein RUB2Add BLAST76
PropeptideiPRO_0000035968153 – 154Curated2

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki76Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)PROSITE-ProRule annotation
Cross-linki152Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)PROSITE-ProRule annotation

Keywords - PTMi

Isopeptide bond

Proteomic databases

PaxDbiQ8RUC6.
PRIDEiQ8RUC6.

PTM databases

iPTMnetiQ8RUC6.

Expressioni

Tissue specificityi

Expressed in leaves, stems and flowers.1 Publication

Gene expression databases

ExpressionAtlasiQ8RUC6. baseline and differential.
GenevisibleiQ8RUC6. AT.

Interactioni

Subunit structurei

NEDD8-like protein RUB2: Forms a thiol ester with the heterodimer AXR1/ECR1.By similarity

Protein-protein interaction databases

BioGridi3477. 3 interactors.
STRINGi3702.AT2G35635.1.

Structurei

3D structure databases

ProteinModelPortaliQ8RUC6.
SMRiQ8RUC6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 76Ubiquitin-like 1PROSITE-ProRule annotationAdd BLAST76
Domaini77 – 152Ubiquitin-like 2PROSITE-ProRule annotationAdd BLAST76

Sequence similaritiesi

Belongs to the ubiquitin family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0001. Eukaryota.
COG5272. LUCA.
InParanoidiQ8RUC6.
KOiK12158.
OMAiINIEPND.
OrthoDBiEOG09360QBF.

Family and domain databases

InterProiView protein in InterPro
IPR019956. Ubiquitin.
IPR029071. Ubiquitin-rel_dom.
IPR019954. Ubiquitin_CS.
IPR000626. Ubiquitin_dom.
PfamiView protein in Pfam
PF00240. ubiquitin. 2 hits.
PRINTSiPR00348. UBIQUITIN.
SMARTiView protein in SMART
SM00213. UBQ. 2 hits.
SUPFAMiSSF54236. SSF54236. 2 hits.
PROSITEiView protein in PROSITE
PS00299. UBIQUITIN_1. 2 hits.
PS50053. UBIQUITIN_2. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8RUC6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQIFVKTLTG KTITLEVESS DTIDNVKAKI QDKEGIPPDQ QRLIFAGKQL
60 70 80 90 100
EDGRTLADYN IQKESTLHLV LRLRGGTMIK VKTLTGKEIE IDIEPTDTID
110 120 130 140 150
RIKERVEEKE GIPPVQQRLI YAGKQLADDK TAKDYAIEGG SVLHLVLALR

GGLL
Length:154
Mass (Da):17,150
Last modified:August 10, 2010 - v3
Checksum:iDF75521A63AEF7CA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006068 Genomic DNA. Translation: AAM15116.1.
CP002685 Genomic DNA. Translation: AEC09134.1.
AK118678 mRNA. Translation: BAC43273.1.
AY074850 mRNA. Translation: AAL75902.1.
AY093802 mRNA. Translation: AAM10418.1.
PIRiS55242.
RefSeqiNP_565812.1. NM_129118.5.
UniGeneiAt.37643.

Genome annotation databases

EnsemblPlantsiAT2G35635.1; AT2G35635.1; AT2G35635.
GeneIDi818132.
GrameneiAT2G35635.1; AT2G35635.1; AT2G35635.
KEGGiath:AT2G35635.

Similar proteinsi

Entry informationi

Entry nameiRUB2_ARATH
AccessioniPrimary (citable) accession number: Q8RUC6
Secondary accession number(s): O80715
, P59263, Q38875, Q9LDJ2, Q9LYW1, Q9M0W3, Q9M1P9, Q9S7X3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: August 10, 2010
Last modified: September 27, 2017
This is version 110 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families