Q8RTL4 (Q8RTL4_9BURK) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Oxygenase-alpha NBDO EMBL AAL76202.1

Gene names

Name:

nbzAc EMBL AAL76202.1

Organism

Comamonas sp. JS765 EMBL AAL76202.1

Taxonomic identifier

58226 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Comamonas

Protein attributes

Sequence length	447 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Sequence similarities

Contains 1 Rieske domain. RuleBase RU004493

Ontologies

Keywords
Ligand	2Fe-2S RuleBase RU004492 PDB 2BMO PDB 2BMQ PDB 2BMR Iron Iron-sulfur Metal-binding
Technical term	3D-structure PDB 2BMO PDB 2BMQ PDB 2BMR
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NADH or NADPH as one donor, and incorporation of two atoms of oxygen into one donor Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction Ref.2. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
nbzAd	Q8RTL3	3	EBI-1042204,EBI-1042214

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	79	1	Iron-sulfur (2Fe-2S) PDB 2BMO PDB 2BMQ PDB 2BMR
	Metal binding	81	1	Iron-sulfur (2Fe-2S); via pros nitrogen PDB 2BMO PDB 2BMQ PDB 2BMR
	Metal binding	99	1	Iron-sulfur (2Fe-2S) PDB 2BMO PDB 2BMQ PDB 2BMR
	Metal binding	102	1	Iron-sulfur (2Fe-2S); via pros nitrogen PDB 2BMO PDB 2BMQ PDB 2BMR
	Metal binding	206	1	Iron; via tele nitrogen PDB 2BMO PDB 2BMQ PDB 2BMR
	Metal binding	211	1	Iron; via tele nitrogen PDB 2BMO PDB 2BMQ PDB 2BMR
	Metal binding	360	1	Iron PDB 2BMO PDB 2BMQ PDB 2BMR

Sequences

	Sequence		Length	Mass (Da)
	Q8RTL4 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 4CA961F99A37DF3A	FASTA	447	49,557
	10 20 30 40 50 60 MSYQNLVSEA GLTQKLLIHG DKELFQHELK TIFARNWLFL THDSLIPSPG DYVKAKMGVD 70 80 90 100 110 120 EVIVSRQNDG SVRAFLNVCR HRGKTLVHAE AGNAKGFVCG YHGWGYGSNG ELQSVPFEKE 130 140 150 160 170 180 LYGDAIKKKC LGLKEVPRIE SFHGFIYGCF DAEAPPLIDY LGDAAWYLEP TFKYSGGLEL 190 200 210 220 230 240 VGPPGKVVVK ANWKSFAENF VGDGYHVGWT HAAALRAGQS VFSSIAGNAK LPPEGAGLQM 250 260 270 280 290 300 TSKYGSGMGV FWGYYSGNFS ADMIPDLMAF GAAKQEKLAK EIGDVRARIY RSFLNGTIFP 310 320 330 340 350 360 NNSFLTGSAA FRVWNPIDEN TTEVWTYAFV EKDMPEDLKR RVADAVQRSI GPAGFWESDD 370 380 390 400 410 420 NENMETMSQN GKKYQSSNID QIASLGFGKD VYGDECYPGV VGKSAIGETS YRGFYRAYQA 430 440 HISSSNWAEF ENASRNWHIE HTKTTDR

References

[1]	"Molecular characterization and substrate specificity of nitrobenzene dioxygenase from Comamonas sp. strain JS765." Lessner D.J., Johnson G.R., Parales R.E., Spain J.C., Gibson D.T. Appl. Environ. Microbiol. 68:634-641(2002) [PubMed: 11823201] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: JS765 EMBL AAL76202.1.
[2]	"Structural insight into the dioxygenation of nitroarene compounds: the crystal structure of nitrobenzene dioxygenase." Friemann R., Ivkovic-Jensen M.M., Lessner D.J., Yu C.L., Gibson D.T., Parales R.E., Eklund H., Ramaswamy S. J. Mol. Biol. 348:1139-1151(2005) [PubMed: 15854650] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.20 ANGSTROMS) IN COMPLEX WITH IRON AND IRON-SULFUR (2FE-2S).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF379638 Genomic DNA. Translation: AAL76202.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2BMO	X-ray	1.20	A	1-447	[»]
2BMQ	X-ray	1.55	A	1-447	[»]
2BMR	X-ray	1.50	A	1-447	[»]

ProteinModelPortal

Q8RTL4.

SMR

Q8RTL4. Positions 3-439.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q8RTL4. 1 interaction.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR017941. Rieske_2Fe-2S.
IPR015881. Ring-hydroxy_dOase_2Fe2S_BS.
IPR015879. Ring_hydroxy_dOase_asu_C.
IPR001663. Rng_hydr_dOase-A.
[Graphical view]

Gene3D

G3DSA:2.102.10.10. Rieske_reg. 1 hit.

Pfam

PF00355. Rieske. 1 hit.
PF00848. Ring_hydroxyl_A. 1 hit.
[Graphical view]

PRINTS

PR00090. RNGDIOXGNASE.

SUPFAM

SSF50022. Rieske_dom. 1 hit.

PROSITE

PS51296. RIESKE. 1 hit.
PS00570. RING_HYDROXYL_ALPHA. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

Q8RTL4_9BURK

Accession

Primary (citable) accession number: Q8RTL4

Entry history

Integrated into UniProtKB/TrEMBL:	June 1, 2002
Last sequence update:	June 1, 2002
Last modified:	October 19, 2011
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)