Q8RT53 (Q8RT53_GEOSE) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Endonuclease Bse634IR EMBL AAL03948.1

Gene names

Name:

bse634IR EMBL AAL03948.1

Organism

Geobacillus stearothermophilus (Bacillus stearothermophilus) EMBL AAL03948.1

Taxonomic identifier

1422 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	293 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Molecular function	Endonuclease EMBL AAL03948.1 Hydrolase Nuclease
Technical term	3D-structure PDB 1KNV PDB 3V1Z PDB 3V20 PDB 3V21
Gene Ontology (GO)
Biological_process	DNA restriction-modification system Inferred from electronic annotation. Source: InterPro
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q8RT53 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: A8C81C0E6320F6A0	FASTA	293	33,799
	10 20 30 40 50 60 MTTNLTNSNC VEEYKENGKT KIRIKPFNAL IELYHHQTPT GSIKENLDKL ENYVKDVVKA 70 80 90 100 110 120 KGLAIPTSGA FSNTRGTWFE VMIAIQSWNY RVKRELNDYL IIKMPNVKTL HFRKIFDNET 130 140 150 160 170 180 REKLHQLEKT LLTHKQQVRL ITSNPDLLII RQKDLIKSEY NLPINKLTHE NIDVALTLFK 190 200 210 220 230 240 DIEGKCKWDS LVAGVGLKTS LRPDRRLQLV HEGNILKSLF AHLKMRYWNP KAEFKYYGAS 250 260 270 280 290 SEPVSKADDD ALQTAATHTI VNVNSTPERA VDDIFSLTSF EDIDKMLDQI IKK

References

[1]	"Crystal structure of the Bse634I restriction endonuclease: comparison of two enzymes recognizing the same DNA sequence." Grazulis S., Deibert M., Rimseliene R., Skirgaila R., Sasnauskas G., Lagunavicius A., Repin V., Urbanke C., Huber R., Siksnys V. Nucleic Acids Res. 30:876-885(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"Structural mechanisms of the degenerate sequence recognition by Bse634I restriction endonuclease." Manakova E., Grazulis S., Zaremba M., Tamulaitiene G., Golovenko D., Siksnys V. Nucleic Acids Res. 40:6741-6751(2012) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AY046877 Genomic DNA. Translation: AAL03948.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1KNV	X-ray	2.17	A/B	1-293	[»]
3V1Z	X-ray	2.20	A/B	1-293	[»]
3V20	X-ray	2.35	A/B	1-293	[»]
3V21	X-ray	2.70	A/B/C/D/E/F/G/H	1-293	[»]

ProteinModelPortal

Q8RT53.

SMR

Q8RT53. Positions 3-293.

ModBase

Search...

Protein family/group databases

REBASE

2800. Bse634I.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

3.40.91.10. 1 hit.

InterPro

IPR011335. Restrct_endonuc-II-like.
IPR011544. Restrct_endonuc_II_Cfr10/BsoB1.
IPR012415. Restrct_endonuc_II_Cfr10I.
[Graphical view]

Pfam

PF07832. Bse634I. 1 hit.
[Graphical view]

SUPFAM

SSF52980. Restrict_endonuc_II-like_core. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

Q8RT53.

Entry information

Entry name

Q8RT53_GEOSE

Accession

Primary (citable) accession number: Q8RT53

Entry history

Integrated into UniProtKB/TrEMBL:	June 1, 2002
Last sequence update:	June 1, 2002
Last modified:	April 3, 2013
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)