ID   GUN_PSEFS               Reviewed;         398 AA.
AC   Q8RSY9; C3K5V6;
DT   26-JUL-2002, integrated into UniProtKB/Swiss-Prot.
DT   26-JUL-2002, sequence version 2.
DT   27-MAR-2024, entry version 131.
DE   RecName: Full=Endoglucanase;
DE            EC=3.2.1.4;
DE   AltName: Full=Carboxymethylcellulase;
DE            Short=CMCase;
DE   AltName: Full=Cellulase;
DE   AltName: Full=Endo-1,4-beta-glucanase;
DE   Flags: Precursor;
GN   Name=bcsZ; Synonyms=wssD; OrderedLocusNames=PFLU_0303;
OS   Pseudomonas fluorescens (strain SBW25).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=216595;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=12019221; DOI=10.1093/genetics/161.1.33;
RA   Spiers A.J., Kahn S.G., Bohannon J., Travisano M., Rainey P.B.;
RT   "Adaptive divergence in experimental populations of Pseudomonas
RT   fluorescens. I. Genetic and phenotypic bases of wrinkly spreader fitness.";
RL   Genetics 161:33-46(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SBW25;
RX   PubMed=19432983; DOI=10.1186/gb-2009-10-5-r51;
RA   Silby M.W., Cerdeno-Tarraga A.M., Vernikos G.S., Giddens S.R.,
RA   Jackson R.W., Preston G.M., Zhang X.-X., Moon C.D., Gehrig S.M.,
RA   Godfrey S.A.C., Knight C.G., Malone J.G., Robinson Z., Spiers A.J.,
RA   Harris S., Challis G.L., Yaxley A.M., Harris D., Seeger K., Murphy L.,
RA   Rutter S., Squares R., Quail M.A., Saunders E., Mavromatis K.,
RA   Brettin T.S., Bentley S.D., Hothersall J., Stephens E., Thomas C.M.,
RA   Parkhill J., Levy S.B., Rainey P.B., Thomson N.R.;
RT   "Genomic and genetic analyses of diversity and plant interactions of
RT   Pseudomonas fluorescens.";
RL   Genome Biol. 10:R51.1-R51.16(2009).
CC   -!- FUNCTION: Hydrolyzes carboxymethylcellulose. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC         cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC   -!- PATHWAY: Glycan metabolism; bacterial cellulose biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 8 (cellulase D) family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAL71844.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AY074776; AAL71844.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AM181176; CAY46580.1; -; Genomic_DNA.
DR   RefSeq; WP_012721724.1; NC_012660.1.
DR   AlphaFoldDB; Q8RSY9; -.
DR   SMR; Q8RSY9; -.
DR   CAZy; GH8; Glycoside Hydrolase Family 8.
DR   PATRIC; fig|216595.4.peg.536; -.
DR   eggNOG; COG3405; Bacteria.
DR   HOGENOM; CLU_037297_0_0_6; -.
DR   OrthoDB; 9766708at2; -.
DR   UniPathway; UPA00694; -.
DR   Proteomes; UP000002332; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 1.50.10.10; -; 1.
DR   InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR   InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR   InterPro; IPR002037; Glyco_hydro_8.
DR   InterPro; IPR019834; Glyco_hydro_8_CS.
DR   Pfam; PF01270; Glyco_hydro_8; 1.
DR   PRINTS; PR00735; GLHYDRLASE8.
DR   SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR   PROSITE; PS00812; GLYCOSYL_HYDROL_F8; 1.
PE   3: Inferred from homology;
KW   Carbohydrate metabolism; Cellulose degradation; Glycosidase; Hydrolase;
KW   Polysaccharide degradation; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..398
FT                   /note="Endoglucanase"
FT                   /id="PRO_0000007942"
FT   ACT_SITE        58
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        119
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10058"
SQ   SEQUENCE   398 AA;  43345 MW;  0DF6CD0671071FC9 CRC64;
     MSPLKCMALA ALGAVMFVGS AQAQTCDWPL WQNYAKRFVQ DDGRVLNSSM KPTESSSEGQ
     SYAMFFALVG NDRASFDKLW TWTKANMSGA DIGQNLPGWL WGKKADNTWG VIDPNSASDA
     DLWMAYALLE AARVWNAPQY RADAQLLLAN VERNLIVRVP GLGKMLLPGP VGYVHAGGLW
     RFNPSYQVLA QLRRFHKERP NAGWNEVADS NAKMLADTAS NPHGLAANWV GYRATSANTG
     LFVVDPFSDD LGSYDAIRTY MWAGMTAKGD PLAAPMLKSL GGMTRATAAS ATGYPPEKIH
     VLTGEVEKNN GYTPMGFSAS TVAFFQARGE TALAQLQKAK VDDALAKALA PSAPDTAQPI
     YYDYMLSLFS QGFADQKYRF EQDGTVKLSW EAACAVTR
//