Q8RSY9 (GUN_PSEFS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoglucanase
EC=3.2.1.4

Alternative name(s):
Carboxymethylcellulase
Short name=CMCase
Cellulase
Endo-1,4-beta-glucanase

Gene names

Name:	bcsZ
Synonyms:	wssD
Ordered Locus Names:	PFLU_0303

Organism

Pseudomonas fluorescens (strain SBW25) [Complete proteome] [HAMAP]

Taxonomic identifier

216595 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas fluorescens group › Pseudomonas fluorescens

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Hydrolyzes carboxymethylcellulose By similarity.
Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Pathway	Glycan metabolism; bacterial cellulose biosynthesis.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 8 (cellulase D) family.
Sequence caution	The sequence AAL71844.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 398	375	Endoglucanase		PRO_0000007942
Sites
	Active site	58	1	Proton donor By similarity
	Active site	119	1	Nucleophile Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8RSY9 [UniParc]. Last modified July 26, 2002. Version 2. Checksum: 0DF6CD0671071FC9	FASTA	398	43,345
	10 20 30 40 50 60 MSPLKCMALA ALGAVMFVGS AQAQTCDWPL WQNYAKRFVQ DDGRVLNSSM KPTESSSEGQ 70 80 90 100 110 120 SYAMFFALVG NDRASFDKLW TWTKANMSGA DIGQNLPGWL WGKKADNTWG VIDPNSASDA 130 140 150 160 170 180 DLWMAYALLE AARVWNAPQY RADAQLLLAN VERNLIVRVP GLGKMLLPGP VGYVHAGGLW 190 200 210 220 230 240 RFNPSYQVLA QLRRFHKERP NAGWNEVADS NAKMLADTAS NPHGLAANWV GYRATSANTG 250 260 270 280 290 300 LFVVDPFSDD LGSYDAIRTY MWAGMTAKGD PLAAPMLKSL GGMTRATAAS ATGYPPEKIH 310 320 330 340 350 360 VLTGEVEKNN GYTPMGFSAS TVAFFQARGE TALAQLQKAK VDDALAKALA PSAPDTAQPI 370 380 390 YYDYMLSLFS QGFADQKYRF EQDGTVKLSW EAACAVTR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Adaptive divergence in experimental populations of Pseudomonas fluorescens. I. Genetic and phenotypic bases of wrinkly spreader fitness." Spiers A.J., Kahn S.G., Bohannon J., Travisano M., Rainey P.B. Genetics 161:33-46(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Genomic and genetic analyses of diversity and plant interactions of Pseudomonas fluorescens." Silby M.W., Cerdeno-Tarraga A.M., Vernikos G.S., Giddens S.R., Jackson R.W., Preston G.M., Zhang X.-X., Moon C.D., Gehrig S.M., Godfrey S.A.C., Knight C.G., Malone J.G., Robinson Z., Spiers A.J., Harris S., Challis G.L., Yaxley A.M., Harris D. , Seeger K., Murphy L., Rutter S., Squares R., Quail M.A., Saunders E., Mavromatis K., Brettin T.S., Bentley S.D., Hothersall J., Stephens E., Thomas C.M., Parkhill J., Levy S.B., Rainey P.B., Thomson N.R. Genome Biol. 10:R51.1-R51.16(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SBW25.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY074776 Genomic DNA. Translation: AAL71844.1. Different initiation. AM181176 Genomic DNA. Translation: CAY46580.1.
RefSeq	YP_002869984.1. NC_012660.1.
3D structure databases
ProteinModelPortal	Q8RSY9.
ModBase	Search...
Protein-protein interaction databases
STRING	216595.PFLU0303.
Protein family/group databases
CAZy	GH8. Glycoside Hydrolase Family 8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAY46580; CAY46580; PFLU_0303.
GeneID	7816407.
KEGG	pfs:PFLU0303.
PATRIC	19894810. VBIPseFlu98510_0536.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3405.
KO	K01179.
OMA	IRVYLWV.
ProtClustDB	PRK11097.
Enzyme and pathway databases
UniPathway	UPA00694.
Family and domain databases
Gene3D	1.50.10.10. 1 hit.
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR012341. 6hp_glycosidase. IPR002037. Glyco_hydro_8. IPR019834. Glyco_hydro_8_CS. [Graphical view]
Pfam	PF01270. Glyco_hydro_8. 1 hit. [Graphical view]
PRINTS	PR00735. GLHYDRLASE8.
SUPFAM	SSF48208. Glyco_trans_6hp. 1 hit.
PROSITE	PS00812. GLYCOSYL_HYDROL_F8. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN_PSEFS

Accession

Primary (citable) accession number: Q8RSY9
Secondary accession number(s): C3K5V6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 26, 2002
Last sequence update:	July 26, 2002
Last modified:	May 1, 2013
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program