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Q8RSY9 (GUN_PSEFS) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endoglucanase

EC=3.2.1.4
Alternative name(s):
Carboxymethylcellulase
Short name=CMCase
Cellulase
Endo-1,4-beta-glucanase
Gene names
Name:bcsZ
Synonyms:wssD
Ordered Locus Names:PFLU_0303
OrganismPseudomonas fluorescens (strain SBW25) [Complete proteome] [HAMAP]
Taxonomic identifier216595 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length398 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Hydrolyzes carboxymethylcellulose By similarity.

Catalytic activity

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Pathway

Glycan metabolism; bacterial cellulose biosynthesis.

Subcellular location

Secreted By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 8 (cellulase D) family.

Sequence caution

The sequence AAL71844.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Cellulose degradation
Polysaccharide degradation
   Cellular componentSecreted
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processcellulose catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncellulase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 398375Endoglucanase
PRO_0000007942

Sites

Active site581Proton donor By similarity
Active site1191Nucleophile Potential

Sequences

Sequence LengthMass (Da)Tools
Q8RSY9 [UniParc].

Last modified July 26, 2002. Version 2.
Checksum: 0DF6CD0671071FC9

FASTA39843,345
        10         20         30         40         50         60 
MSPLKCMALA ALGAVMFVGS AQAQTCDWPL WQNYAKRFVQ DDGRVLNSSM KPTESSSEGQ 

        70         80         90        100        110        120 
SYAMFFALVG NDRASFDKLW TWTKANMSGA DIGQNLPGWL WGKKADNTWG VIDPNSASDA 

       130        140        150        160        170        180 
DLWMAYALLE AARVWNAPQY RADAQLLLAN VERNLIVRVP GLGKMLLPGP VGYVHAGGLW 

       190        200        210        220        230        240 
RFNPSYQVLA QLRRFHKERP NAGWNEVADS NAKMLADTAS NPHGLAANWV GYRATSANTG 

       250        260        270        280        290        300 
LFVVDPFSDD LGSYDAIRTY MWAGMTAKGD PLAAPMLKSL GGMTRATAAS ATGYPPEKIH 

       310        320        330        340        350        360 
VLTGEVEKNN GYTPMGFSAS TVAFFQARGE TALAQLQKAK VDDALAKALA PSAPDTAQPI 

       370        380        390 
YYDYMLSLFS QGFADQKYRF EQDGTVKLSW EAACAVTR 

« Hide

References

« Hide 'large scale' references
[1]"Adaptive divergence in experimental populations of Pseudomonas fluorescens. I. Genetic and phenotypic bases of wrinkly spreader fitness."
Spiers A.J., Kahn S.G., Bohannon J., Travisano M., Rainey P.B.
Genetics 161:33-46(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Genomic and genetic analyses of diversity and plant interactions of Pseudomonas fluorescens."
Silby M.W., Cerdeno-Tarraga A.M., Vernikos G.S., Giddens S.R., Jackson R.W., Preston G.M., Zhang X.-X., Moon C.D., Gehrig S.M., Godfrey S.A.C., Knight C.G., Malone J.G., Robinson Z., Spiers A.J., Harris S., Challis G.L., Yaxley A.M., Harris D. expand/collapse author list , Seeger K., Murphy L., Rutter S., Squares R., Quail M.A., Saunders E., Mavromatis K., Brettin T.S., Bentley S.D., Hothersall J., Stephens E., Thomas C.M., Parkhill J., Levy S.B., Rainey P.B., Thomson N.R.
Genome Biol. 10:R51.1-R51.16(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SBW25.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY074776 Genomic DNA. Translation: AAL71844.1. Different initiation.
AM181176 Genomic DNA. Translation: CAY46580.1.
RefSeqYP_002869984.1. NC_012660.1.

3D structure databases

ProteinModelPortalQ8RSY9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING216595.PFLU0303.

Protein family/group databases

CAZyGH8. Glycoside Hydrolase Family 8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAY46580; CAY46580; PFLU_0303.
GeneID7816407.
KEGGpfs:PFLU0303.
PATRIC19894810. VBIPseFlu98510_0536.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG3405.
KOK01179.
OMADLWIAYT.
OrthoDBEOG6DG2PM.

Enzyme and pathway databases

UniPathwayUPA00694.

Family and domain databases

Gene3D1.50.10.10. 1 hit.
InterProIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR002037. Glyco_hydro_8.
IPR019834. Glyco_hydro_8_CS.
[Graphical view]
PfamPF01270. Glyco_hydro_8. 1 hit.
[Graphical view]
PRINTSPR00735. GLHYDRLASE8.
SUPFAMSSF48208. SSF48208. 1 hit.
PROSITEPS00812. GLYCOSYL_HYDROL_F8. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGUN_PSEFS
AccessionPrimary (citable) accession number: Q8RSY9
Secondary accession number(s): C3K5V6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 26, 2002
Last sequence update: July 26, 2002
Last modified: May 14, 2014
This is version 72 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries