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Protein

Endoglucanase

Gene

bcsZ

Organism
Pseudomonas fluorescens (strain SBW25)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Hydrolyzes carboxymethylcellulose.By similarity

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Pathwayi: bacterial cellulose biosynthesis

This protein is involved in the pathway bacterial cellulose biosynthesis, which is part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the pathway bacterial cellulose biosynthesis and in Glycan metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei58Proton donorBy similarity1
Active sitei119NucleophilePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

UniPathwayiUPA00694.

Protein family/group databases

CAZyiGH8. Glycoside Hydrolase Family 8.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase (EC:3.2.1.4)
Alternative name(s):
Carboxymethylcellulase
Short name:
CMCase
Cellulase
Endo-1,4-beta-glucanase
Gene namesi
Name:bcsZ
Synonyms:wssD
Ordered Locus Names:PFLU_0303
OrganismiPseudomonas fluorescens (strain SBW25)
Taxonomic identifieri216595 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002332 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000000794224 – 398EndoglucanaseAdd BLAST375

Proteomic databases

PRIDEiQ8RSY9.

Interactioni

Protein-protein interaction databases

STRINGi216595.PFLU0303.

Structurei

3D structure databases

ProteinModelPortaliQ8RSY9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105K2Z. Bacteria.
COG3405. LUCA.
KOiK20542.
OMAiIRVYLWV.
OrthoDBiPOG091H07QZ.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR002037. Glyco_hydro_8.
IPR019834. Glyco_hydro_8_CS.
[Graphical view]
PfamiPF01270. Glyco_hydro_8. 1 hit.
[Graphical view]
PRINTSiPR00735. GLHYDRLASE8.
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00812. GLYCOSYL_HYDROL_F8. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8RSY9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPLKCMALA ALGAVMFVGS AQAQTCDWPL WQNYAKRFVQ DDGRVLNSSM
60 70 80 90 100
KPTESSSEGQ SYAMFFALVG NDRASFDKLW TWTKANMSGA DIGQNLPGWL
110 120 130 140 150
WGKKADNTWG VIDPNSASDA DLWMAYALLE AARVWNAPQY RADAQLLLAN
160 170 180 190 200
VERNLIVRVP GLGKMLLPGP VGYVHAGGLW RFNPSYQVLA QLRRFHKERP
210 220 230 240 250
NAGWNEVADS NAKMLADTAS NPHGLAANWV GYRATSANTG LFVVDPFSDD
260 270 280 290 300
LGSYDAIRTY MWAGMTAKGD PLAAPMLKSL GGMTRATAAS ATGYPPEKIH
310 320 330 340 350
VLTGEVEKNN GYTPMGFSAS TVAFFQARGE TALAQLQKAK VDDALAKALA
360 370 380 390
PSAPDTAQPI YYDYMLSLFS QGFADQKYRF EQDGTVKLSW EAACAVTR
Length:398
Mass (Da):43,345
Last modified:July 26, 2002 - v2
Checksum:i0DF6CD0671071FC9
GO

Sequence cautioni

The sequence AAL71844 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY074776 Genomic DNA. Translation: AAL71844.1. Different initiation.
AM181176 Genomic DNA. Translation: CAY46580.1.
RefSeqiWP_012721724.1. NC_012660.1.

Genome annotation databases

EnsemblBacteriaiCAY46580; CAY46580; PFLU_0303.
GeneIDi7816407.
KEGGipfs:PFLU_0303.
PATRICi19894810. VBIPseFlu98510_0536.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY074776 Genomic DNA. Translation: AAL71844.1. Different initiation.
AM181176 Genomic DNA. Translation: CAY46580.1.
RefSeqiWP_012721724.1. NC_012660.1.

3D structure databases

ProteinModelPortaliQ8RSY9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi216595.PFLU0303.

Protein family/group databases

CAZyiGH8. Glycoside Hydrolase Family 8.

Proteomic databases

PRIDEiQ8RSY9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAY46580; CAY46580; PFLU_0303.
GeneIDi7816407.
KEGGipfs:PFLU_0303.
PATRICi19894810. VBIPseFlu98510_0536.

Phylogenomic databases

eggNOGiENOG4105K2Z. Bacteria.
COG3405. LUCA.
KOiK20542.
OMAiIRVYLWV.
OrthoDBiPOG091H07QZ.

Enzyme and pathway databases

UniPathwayiUPA00694.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR002037. Glyco_hydro_8.
IPR019834. Glyco_hydro_8_CS.
[Graphical view]
PfamiPF01270. Glyco_hydro_8. 1 hit.
[Graphical view]
PRINTSiPR00735. GLHYDRLASE8.
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00812. GLYCOSYL_HYDROL_F8. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGUN_PSEFS
AccessioniPrimary (citable) accession number: Q8RSY9
Secondary accession number(s): C3K5V6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 26, 2002
Last sequence update: July 26, 2002
Last modified: November 2, 2016
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.