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Protein

Effector protein HopAB2

Gene

hopAB2

Organism
Pseudomonas syringae pv. tomato (strain DC3000)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Effector protein involved in gene-for-gene resistance in tomato plants. It is recognized by the host Pto resistance protein and elicits Pto and Prf-dependent hypersensitive response (HR) and programmed cell death (PCD), resulting in host immunity. In susceptible plants, acts as a virulence factor by suppressing PCD and HR-based plant immunity. This function requires its E3 ubiquitin ligase activity probably by recruiting E2 enzymes and transferring ubiquitin molecules to cellular proteins involved in regulation of PCD and targeting them for degradation. Also, induces expression of host genes involved in ethylene biosynthesis and signaling, in particular ACO1 and ACO2, encoding the ethylene-forming enzyme ACC oxidase.5 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei479 – 4791E2-bindingCurated
Sitei525 – 5251E2-bindingCurated
Sitei533 – 5331E2-bindingCurated

GO - Molecular functioni

GO - Biological processi

  • modulation by symbiont of host defense-related programmed cell death Source: UniProtKB-KW
  • modulation by symbiont of RNA levels in host Source: JCVI
  • pathogenesis Source: CACAO
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Hypersensitive response elicitation, Ubl conjugation pathway, Virulence

Enzyme and pathway databases

BioCyciPSYR223283:GJIX-3135-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Effector protein HopAB2
Alternative name(s):
Avirulence protein AvrPtoB
Including the following 1 domains:
E3 ubiquitin-protein ligase (EC:6.3.2.-)
Gene namesi
Name:hopAB2
Synonyms:avrPtoB
Ordered Locus Names:PSPTO_3087
OrganismiPseudomonas syringae pv. tomato (strain DC3000)
Taxonomic identifieri223283 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002515 Componenti: Chromosome

Subcellular locationi

  • Secreted 1 Publication

  • Note: Secreted via type III secretion system (TTSS). Localized to the plant cell cytoplasm.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi325 – 3251G → A: Reduces interaction with Pto and fails to elicit Pto-dependent defense response in tomato leaves. 1 Publication
Mutagenesisi326 – 3261I → T: Reduces interaction with Pto and fails to elicit Pto-dependent defense response in tomato leaves. 1 Publication
Mutagenesisi327 – 3271N → A: Reduces interaction with Pto. 1 Publication
Mutagenesisi331 – 3311D → A: No effect in the interaction with Pto. 1 Publication
Mutagenesisi333 – 3331G → A: No effect in the interaction with Pto and no effect in the induction of Pto-dependent defense response. 1 Publication
Mutagenesisi453 – 4531K → R: No effect in ubiquitination. 1 Publication
Mutagenesisi464 – 4641K → R: No effect in ubiquitination. 1 Publication
Mutagenesisi479 – 4791F → A: Loses ubiquitination activity.
Mutagenesisi512 – 5121K → R: 5- to 50-fold reduction in ubiquitin interactions. Suppress PCD in susceptible tomato leaves. 1 Publication
Mutagenesisi520 – 5212KK → RR: 5- to 50-fold reduction in ubiquitin interactions. Suppress PCD in susceptible tomato leaves. 1 Publication
Mutagenesisi525 – 5251F → A: Loses ubiquitination activity.
Mutagenesisi529 – 5291K → R: 5- to 50-fold reduction in ubiquitin interactions. Suppress PCD in susceptible tomato leaves. 1 Publication
Mutagenesisi533 – 5331P → A: Loses ubiquitination activity. 1 Publication
Mutagenesisi546 – 5461K → R: No effect in ubiquitination. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 553553Effector protein HopAB2PRO_0000234081Add
BLAST

Post-translational modificationi

Auto-ubiquitinated.

Keywords - PTMi

Ubl conjugation

Expressioni

Inductioni

Transcriptionally induced by HrpL.1 Publication

Interactioni

Subunit structurei

Interacts physically with plant cell Pto.

Protein-protein interaction databases

DIPiDIP-60253N.
STRINGi223283.PSPTO_3087.

Structurei

Secondary structure

1
553
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi125 – 13814Combined sources
Helixi143 – 15412Combined sources
Helixi163 – 17210Combined sources
Helixi176 – 1794Combined sources
Helixi186 – 20015Combined sources
Helixi271 – 29121Combined sources
Helixi296 – 30712Combined sources
Helixi315 – 3239Combined sources
Helixi340 – 35112Combined sources
Helixi442 – 4476Combined sources
Beta strandi448 – 4525Combined sources
Beta strandi480 – 4823Combined sources
Beta strandi492 – 4987Combined sources
Beta strandi514 – 5185Combined sources
Helixi519 – 52810Combined sources
Turni533 – 5353Combined sources
Turni541 – 5433Combined sources
Helixi544 – 5474Combined sources
Beta strandi548 – 5525Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2FD4X-ray1.80A436-553[»]
3HGKX-ray3.30E/F/G/H121-205[»]
3HGLX-ray1.90A121-205[»]
3TL8X-ray2.50B/F/K/L250-359[»]
ProteinModelPortaliQ8RSY1.
SMRiQ8RSY1. Positions 436-553.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8RSY1.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 308308Host recognition; Pto interactionAdd
BLAST
Regioni309 – 553245E3 ubiquitin-protein ligaseAdd
BLAST
Regioni512 – 52918Required for E3 ubiquitin-protein ligase and anti-PCD activities and pathogenesisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi325 – 3284Interaction with Pto-kinase

Sequence similaritiesi

Belongs to the HopAB family.Curated

Phylogenomic databases

HOGENOMiHOG000112938.
KOiK13452.
OMAiVAFSRVE.
OrthoDBiEOG6384HK.

Family and domain databases

InterProiIPR031759. AvrPtoB_BAK-bd.
IPR015133. E3_ubiquit_lig_AvrPtoB.
[Graphical view]
PfamiPF09046. AvrPtoB-E3_ubiq. 1 hit.
PF16847. AvrPtoB_bdg. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8RSY1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGINRAGPS GAYFVGHTDP EPVSGQAHGS GSGASSSNSP QVQPRPSNTP
60 70 80 90 100
PSNAPAPPPT GRERLSRSTA LSRQTREWLE QGMPTAEDAS VRRRPQVTAD
110 120 130 140 150
AATPRAEARR TPEATADASA PRRGAVAHAN SIVQQLVSEG ADISHTRNML
160 170 180 190 200
RNAMNGDAVA FSRVEQNIFR QHFPNMPMHG ISRDSELAIE LRGALRRAVH
210 220 230 240 250
QQAASAPVRS PTPTPASPAA SSSGSSQRSL FGRFARLMAP NQGRSSNTAA
260 270 280 290 300
SQTPVDRSPP RVNQRPIRVD RAAMRNRGND EADAALRGLV QQGVNLEHLR
310 320 330 340 350
TALERHVMQR LPIPLDIGSA LQNVGINPSI DLGESLVQHP LLNLNVALNR
360 370 380 390 400
MLGLRPSAER APRPAVPVAP ATASRRPDGT RATRLRVMPE REDYENNVAY
410 420 430 440 450
GVRLLNLNPG VGVRQAVAAF VTDRAERPAV VANIRAALDP IASQFSQLRT
460 470 480 490 500
ISKADAESEE LGFKDAADHH TDDVTHCLFG GELSLSNPDQ QVIGLAGNPT
510 520 530 540 550
DTSQPYSQEG NKDLAFMDMK KLAQFLAGKP EHPMTRETLN AENIAKYAFR

IVP
Length:553
Mass (Da):59,453
Last modified:June 1, 2002 - v1
Checksum:i6C638B7DF2D7B399
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY074795 Genomic DNA. Translation: AAL71883.1.
AE016853 Genomic DNA. Translation: AAO56576.1.
RefSeqiNP_792881.1. NC_004578.1.
WP_011104378.1. NC_004578.1.

Genome annotation databases

EnsemblBacteriaiAAO56576; AAO56576; PSPTO_3087.
GeneIDi1184744.
KEGGipst:PSPTO_3087.
PATRICi19997547. VBIPseSyr93040_3153.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY074795 Genomic DNA. Translation: AAL71883.1.
AE016853 Genomic DNA. Translation: AAO56576.1.
RefSeqiNP_792881.1. NC_004578.1.
WP_011104378.1. NC_004578.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2FD4X-ray1.80A436-553[»]
3HGKX-ray3.30E/F/G/H121-205[»]
3HGLX-ray1.90A121-205[»]
3TL8X-ray2.50B/F/K/L250-359[»]
ProteinModelPortaliQ8RSY1.
SMRiQ8RSY1. Positions 436-553.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60253N.
STRINGi223283.PSPTO_3087.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO56576; AAO56576; PSPTO_3087.
GeneIDi1184744.
KEGGipst:PSPTO_3087.
PATRICi19997547. VBIPseSyr93040_3153.

Phylogenomic databases

HOGENOMiHOG000112938.
KOiK13452.
OMAiVAFSRVE.
OrthoDBiEOG6384HK.

Enzyme and pathway databases

BioCyciPSYR223283:GJIX-3135-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ8RSY1.

Family and domain databases

InterProiIPR031759. AvrPtoB_BAK-bd.
IPR015133. E3_ubiquit_lig_AvrPtoB.
[Graphical view]
PfamiPF09046. AvrPtoB-E3_ubiq. 1 hit.
PF16847. AvrPtoB_bdg. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Two distinct Pseudomonas effector proteins interact with the Pto kinase and activate plant immunity."
    Kim Y.-J., Lin N.-C., Martin G.B.
    Cell 109:589-598(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN AVIRULENCE, SUBCELLULAR LOCATION, MUTAGENESIS OF GLY-325; ILE-326; ASN-327; ASP-331 AND GLY-333.
    Strain: DC3000.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DC3000.
  3. Cited for: INDUCTION BY HRPL.
    Strain: DC3000.
  4. "Pseudomonas type III effector AvrPtoB induces plant disease susceptibility by inhibition of host programmed cell death."
    Abramovitch R.B., Kim Y.-J., Chen S., Dickman M.B., Martin G.B.
    EMBO J. 22:60-69(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN VIRULENCE.
    Strain: DC3000.
  5. "Pseudomonas syringae pv. tomato type III effectors AvrPto and AvrPtoB promote ethylene-dependent cell death in tomato."
    Cohn J.R., Martin G.B.
    Plant J. 44:139-154(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN ETHYLENE-DEPENDENT CELL DEATH.
    Strain: DC3000.
  6. "Type III effector AvrPtoB requires intrinsic E3 ubiquitin ligase activity to suppress plant cell death and immunity."
    Abramovitch R.B., Janjusevic R., Stebbins C.E., Martin G.B.
    Proc. Natl. Acad. Sci. U.S.A. 103:2851-2856(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS AN E3 UBIQUITIN-PROTEIN LIGASE.
    Strain: DC3000.
  7. "A bacterial inhibitor of host programmed cell death defenses is an E3 ubiquitin ligase."
    Janjusevic R., Abramovitch R.B., Martin G.B., Stebbins C.E.
    Science 311:222-226(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 436-553, MUTAGENESIS OF LYS-453; LYS-464; LYS-512; 520-LYS-LYS-521; LYS-529; PRO-533 AND LYS-546, FUNCTION AS AN E3 UBIQUITIN-PROTEIN LIGASE.
    Strain: DC3000.

Entry informationi

Entry nameiHPAB2_PSESM
AccessioniPrimary (citable) accession number: Q8RSY1
Secondary accession number(s): Q7C4D6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: June 1, 2002
Last modified: December 9, 2015
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Unlike many effector proteins, it is widely conserved among diverse genera of plant pathogens including Xanthomonas, Erwinia and many strains of Pseudomonas.
Acts as a general eukaryotic PCD inhibitor in plants and yeast.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.