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Protein

Effector protein HopAB2

Gene

hopAB2

Organism
Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Effector protein involved in gene-for-gene resistance in tomato plants. It is recognized by the host Pto resistance protein and elicits Pto and Prf-dependent hypersensitive response (HR) and programmed cell death (PCD), resulting in host immunity. In susceptible plants, acts as a virulence factor by suppressing PCD and HR-based plant immunity. This function requires its E3 ubiquitin ligase activity probably by recruiting E2 enzymes and transferring ubiquitin molecules to cellular proteins involved in regulation of PCD and targeting them for degradation. Also, induces expression of host genes involved in ethylene biosynthesis and signaling, in particular ACO1 and ACO2, encoding the ethylene-forming enzyme ACC oxidase.5 Publications

Miscellaneous

Unlike many effector proteins, it is widely conserved among diverse genera of plant pathogens including Xanthomonas, Erwinia and many strains of Pseudomonas.
Acts as a general eukaryotic PCD inhibitor in plants and yeast.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei479E2-bindingCurated1
Sitei525E2-bindingCurated1
Sitei533E2-bindingCurated1

GO - Molecular functioni

GO - Biological processi

  • modulation by symbiont of host defense-related programmed cell death Source: UniProtKB-KW
  • modulation by symbiont of RNA levels in host Source: JCVI
  • pathogenesis Source: CACAO

Keywordsi

Molecular functionTransferase
Biological processHypersensitive response elicitation, Ubl conjugation pathway, Virulence

Enzyme and pathway databases

BioCyciPSYR223283:G1G0D-3136-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Effector protein HopAB2
Alternative name(s):
Avirulence protein AvrPtoB
Including the following 1 domains:
E3 ubiquitin-protein ligase (EC:2.3.2.-)
Alternative name(s):
E3 ubiquitin-protein transferaseCurated
Gene namesi
Name:hopAB2
Synonyms:avrPtoB
Ordered Locus Names:PSPTO_3087
OrganismiPseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)
Taxonomic identifieri223283 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002515 Componenti: Chromosome

Subcellular locationi

  • Secreted 1 Publication
  • Note: Secreted via type III secretion system (TTSS). Localized to the plant cell cytoplasm.

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi325G → A: Reduces interaction with Pto and fails to elicit Pto-dependent defense response in tomato leaves. 1 Publication1
Mutagenesisi326I → T: Reduces interaction with Pto and fails to elicit Pto-dependent defense response in tomato leaves. 1 Publication1
Mutagenesisi327N → A: Reduces interaction with Pto. 1 Publication1
Mutagenesisi331D → A: No effect in the interaction with Pto. 1 Publication1
Mutagenesisi333G → A: No effect in the interaction with Pto and no effect in the induction of Pto-dependent defense response. 1 Publication1
Mutagenesisi453K → R: No effect in ubiquitination. 1 Publication1
Mutagenesisi464K → R: No effect in ubiquitination. 1 Publication1
Mutagenesisi479F → A: Loses ubiquitination activity. 1
Mutagenesisi512K → R: 5- to 50-fold reduction in ubiquitin interactions. Suppress PCD in susceptible tomato leaves. 1 Publication1
Mutagenesisi520 – 521KK → RR: 5- to 50-fold reduction in ubiquitin interactions. Suppress PCD in susceptible tomato leaves. 1 Publication2
Mutagenesisi525F → A: Loses ubiquitination activity. 1
Mutagenesisi529K → R: 5- to 50-fold reduction in ubiquitin interactions. Suppress PCD in susceptible tomato leaves. 1 Publication1
Mutagenesisi533P → A: Loses ubiquitination activity. 1 Publication1
Mutagenesisi546K → R: No effect in ubiquitination. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002340811 – 553Effector protein HopAB2Add BLAST553

Post-translational modificationi

Auto-ubiquitinated.

Keywords - PTMi

Ubl conjugation

Expressioni

Inductioni

Transcriptionally induced by HrpL.1 Publication

Interactioni

Subunit structurei

Interacts physically with plant cell Pto.

Binary interactionsi

WithEntry#Exp.IntActNotes
UBI3P629803EBI-15569263,EBI-15569288From Solanum lycopersicum.

Protein-protein interaction databases

DIPiDIP-60253N
IntActiQ8RSY1, 4 interactors
STRINGi223283.PSPTO_3087

Structurei

Secondary structure

1553
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi125 – 138Combined sources14
Helixi143 – 154Combined sources12
Helixi163 – 172Combined sources10
Helixi176 – 179Combined sources4
Helixi186 – 200Combined sources15
Helixi271 – 291Combined sources21
Helixi296 – 307Combined sources12
Helixi315 – 323Combined sources9
Helixi340 – 351Combined sources12
Helixi442 – 447Combined sources6
Beta strandi448 – 452Combined sources5
Beta strandi480 – 482Combined sources3
Beta strandi492 – 498Combined sources7
Beta strandi514 – 518Combined sources5
Helixi519 – 528Combined sources10
Turni533 – 535Combined sources3
Turni541 – 543Combined sources3
Helixi544 – 547Combined sources4
Beta strandi548 – 552Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FD4X-ray1.80A436-553[»]
3HGKX-ray3.30E/F/G/H121-205[»]
3HGLX-ray1.90A121-205[»]
3TL8X-ray2.50B/F/K/L250-359[»]
ProteinModelPortaliQ8RSY1
SMRiQ8RSY1
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8RSY1

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 308Host recognition; Pto interactionAdd BLAST308
Regioni309 – 553E3 ubiquitin-protein ligaseAdd BLAST245
Regioni512 – 529Required for E3 ubiquitin-protein ligase and anti-PCD activities and pathogenesisAdd BLAST18

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi325 – 328Interaction with Pto-kinase4

Sequence similaritiesi

Belongs to the HopAB family.Curated

Phylogenomic databases

HOGENOMiHOG000112938
KOiK13452
OMAiDNNAVAF
OrthoDBiPOG091H3TF5

Family and domain databases

CDDicd12803 HopAB_BID, 1 hit
cd12802 HopAB_PID, 1 hit
Gene3Di1.20.1280.220, 1 hit
3.30.40.110, 1 hit
InterProiView protein in InterPro
IPR015133 E3_ubiquit_lig_AvrPtoB
IPR031759 HopAB_BAK-bd
IPR038342 HopAB_BAK-bd_sf
IPR038448 HopAB_E3_ubiquit_lig_sf
IPR033743 HopAB_PID
PfamiView protein in Pfam
PF09046 AvrPtoB-E3_ubiq, 1 hit
PF16847 AvrPtoB_bdg, 1 hit

Sequencei

Sequence statusi: Complete.

Q8RSY1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGINRAGPS GAYFVGHTDP EPVSGQAHGS GSGASSSNSP QVQPRPSNTP
60 70 80 90 100
PSNAPAPPPT GRERLSRSTA LSRQTREWLE QGMPTAEDAS VRRRPQVTAD
110 120 130 140 150
AATPRAEARR TPEATADASA PRRGAVAHAN SIVQQLVSEG ADISHTRNML
160 170 180 190 200
RNAMNGDAVA FSRVEQNIFR QHFPNMPMHG ISRDSELAIE LRGALRRAVH
210 220 230 240 250
QQAASAPVRS PTPTPASPAA SSSGSSQRSL FGRFARLMAP NQGRSSNTAA
260 270 280 290 300
SQTPVDRSPP RVNQRPIRVD RAAMRNRGND EADAALRGLV QQGVNLEHLR
310 320 330 340 350
TALERHVMQR LPIPLDIGSA LQNVGINPSI DLGESLVQHP LLNLNVALNR
360 370 380 390 400
MLGLRPSAER APRPAVPVAP ATASRRPDGT RATRLRVMPE REDYENNVAY
410 420 430 440 450
GVRLLNLNPG VGVRQAVAAF VTDRAERPAV VANIRAALDP IASQFSQLRT
460 470 480 490 500
ISKADAESEE LGFKDAADHH TDDVTHCLFG GELSLSNPDQ QVIGLAGNPT
510 520 530 540 550
DTSQPYSQEG NKDLAFMDMK KLAQFLAGKP EHPMTRETLN AENIAKYAFR

IVP
Length:553
Mass (Da):59,453
Last modified:June 1, 2002 - v1
Checksum:i6C638B7DF2D7B399
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY074795 Genomic DNA Translation: AAL71883.1
AE016853 Genomic DNA Translation: AAO56576.1
RefSeqiNP_792881.1, NC_004578.1
WP_011104378.1, NC_004578.1

Genome annotation databases

EnsemblBacteriaiAAO56576; AAO56576; PSPTO_3087
GeneIDi1184744
KEGGipst:PSPTO_3087
PATRICifig|223283.9.peg.3153

Similar proteinsi

Entry informationi

Entry nameiHPAB2_PSESM
AccessioniPrimary (citable) accession number: Q8RSY1
Secondary accession number(s): Q7C4D6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: June 1, 2002
Last modified: April 25, 2018
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
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