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Q8RSU9

- ALR_CORGL

UniProt

Q8RSU9 - ALR_CORGL

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Protein

Alanine racemase

Gene

alr

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the interconversion of L-alanine and D-alanine. May also act on other amino acids.UniRule annotation

Catalytic activityi

L-alanine = D-alanine.UniRule annotation

Cofactori

Pyridoxal phosphate.1 PublicationUniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei34 – 341Proton acceptor; specific for D-alanineUniRule annotation
Binding sitei129 – 1291SubstrateUniRule annotation
Active sitei256 – 2561Proton acceptor; specific for L-alanineUniRule annotation
Binding sitei304 – 3041Substrate; via amide nitrogenUniRule annotation

GO - Molecular functioni

  1. alanine racemase activity Source: UniProtKB-HAMAP
  2. pyridoxal phosphate binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. D-alanine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00042; UER00497.

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine racemaseUniRule annotation (EC:5.1.1.1UniRule annotation)
Gene namesi
Name:alr
Ordered Locus Names:Cgl0588, cg0681
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
ProteomesiUP000000582: Chromosome, UP000001009: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 361361Alanine racemasePRO_0000114512Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei34 – 341N6-(pyridoxal phosphate)lysine

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi196627.cg0681.

Structurei

Secondary structure

1
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 97
Helixi10 – 2415
Beta strandi27 – 326
Helixi34 – 396
Helixi42 – 5110
Beta strandi56 – 616
Helixi62 – 709
Beta strandi75 – 795
Helixi88 – 925
Turni93 – 953
Beta strandi97 – 1004
Helixi103 – 1108
Beta strandi117 – 1237
Beta strandi129 – 1324
Helixi134 – 14512
Beta strandi150 – 1567
Helixi170 – 18617
Helixi199 – 2046
Helixi206 – 2083
Helixi217 – 2204
Beta strandi236 – 2416
Beta strandi244 – 2474
Beta strandi267 – 2726
Turni275 – 2784
Helixi281 – 2833
Turni284 – 2863
Beta strandi288 – 2914
Beta strandi294 – 3007
Beta strandi307 – 3126
Beta strandi323 – 3286
Helixi334 – 3407
Helixi345 – 3506
Beta strandi357 – 3615

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DY3X-ray2.10A/B/C/D1-361[»]
ProteinModelPortaliQ8RSU9.
SMRiQ8RSU9. Positions 1-361.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8RSU9.

Family & Domainsi

Sequence similaritiesi

Belongs to the alanine racemase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0787.
HOGENOMiHOG000031444.
KOiK01775.
OMAiPSECAGV.
OrthoDBiEOG6PP9NJ.

Family and domain databases

Gene3Di2.40.37.10. 1 hit.
3.20.20.10. 1 hit.
HAMAPiMF_01201. Ala_racemase.
InterProiIPR000821. Ala_racemase.
IPR009006. Ala_racemase/Decarboxylase_C.
IPR011079. Ala_racemase_C.
IPR001608. Ala_racemase_N.
IPR029066. PLP-binding_barrel.
[Graphical view]
PfamiPF00842. Ala_racemase_C. 1 hit.
PF01168. Ala_racemase_N. 1 hit.
[Graphical view]
PRINTSiPR00992. ALARACEMASE.
SMARTiSM01005. Ala_racemase_C. 1 hit.
[Graphical view]
SUPFAMiSSF50621. SSF50621. 1 hit.
SSF51419. SSF51419. 1 hit.
TIGRFAMsiTIGR00492. alr. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8RSU9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNLLTTKIDL DAIAHNTRVL KQMAGPAKLM AVVKANAYNH GVEKVAPVIA
60 70 80 90 100
AHGADAFGVA TLAEAMQLRD IGISQEVLCW IWTPEQDFRA AIDRNIDLAV
110 120 130 140 150
ISPAHAKALI ETDAEHIRVS IKIDSGLHRS GVDEQEWEGV FSALAAAPHI
160 170 180 190 200
EVTGMFTHLA CADEPENPET DRQIIAFRRA LALARKHGLE CPVNHVCNSP
210 220 230 240 250
AFLTRSDLHM EMVRPGLAFY GLEPVAGLEH GLKPAMTWEA KVSVVKQIEA
260 270 280 290 300
GQGTSYGLTW RAEDRGFVAV VPAGYADGMP RHAQGKFSVT IDGLDYPQVG
310 320 330 340 350
RVCMDQFVIS LGDNPHGVEA GAKAVIFGEN GHDATDFAER LDTINYEVVC
360
RPTGRTVRAY V
Length:361
Mass (Da):39,174
Last modified:June 1, 2002 - v1
Checksum:i6E95CAF6456B6037
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY077456 Genomic DNA. Translation: AAL77207.1.
BA000036 Genomic DNA. Translation: BAB97981.1.
BX927149 Genomic DNA. Translation: CAF19293.1.
RefSeqiNP_599824.1. NC_003450.3.
WP_011013748.1. NC_006958.1.
YP_224879.1. NC_006958.1.

Genome annotation databases

EnsemblBacteriaiBAB97981; BAB97981; BAB97981.
CAF19293; CAF19293; cg0681.
GeneIDi1018592.
3343955.
KEGGicgb:cg0681.
cgl:NCgl0563.
PATRICi21493226. VBICorGlu203724_0579.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY077456 Genomic DNA. Translation: AAL77207.1 .
BA000036 Genomic DNA. Translation: BAB97981.1 .
BX927149 Genomic DNA. Translation: CAF19293.1 .
RefSeqi NP_599824.1. NC_003450.3.
WP_011013748.1. NC_006958.1.
YP_224879.1. NC_006958.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2DY3 X-ray 2.10 A/B/C/D 1-361 [» ]
ProteinModelPortali Q8RSU9.
SMRi Q8RSU9. Positions 1-361.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 196627.cg0681.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB97981 ; BAB97981 ; BAB97981 .
CAF19293 ; CAF19293 ; cg0681 .
GeneIDi 1018592.
3343955.
KEGGi cgb:cg0681.
cgl:NCgl0563.
PATRICi 21493226. VBICorGlu203724_0579.

Phylogenomic databases

eggNOGi COG0787.
HOGENOMi HOG000031444.
KOi K01775.
OMAi PSECAGV.
OrthoDBi EOG6PP9NJ.

Enzyme and pathway databases

UniPathwayi UPA00042 ; UER00497 .

Miscellaneous databases

EvolutionaryTracei Q8RSU9.

Family and domain databases

Gene3Di 2.40.37.10. 1 hit.
3.20.20.10. 1 hit.
HAMAPi MF_01201. Ala_racemase.
InterProi IPR000821. Ala_racemase.
IPR009006. Ala_racemase/Decarboxylase_C.
IPR011079. Ala_racemase_C.
IPR001608. Ala_racemase_N.
IPR029066. PLP-binding_barrel.
[Graphical view ]
Pfami PF00842. Ala_racemase_C. 1 hit.
PF01168. Ala_racemase_N. 1 hit.
[Graphical view ]
PRINTSi PR00992. ALARACEMASE.
SMARTi SM01005. Ala_racemase_C. 1 hit.
[Graphical view ]
SUPFAMi SSF50621. SSF50621. 1 hit.
SSF51419. SSF51419. 1 hit.
TIGRFAMsi TIGR00492. alr. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The alanine racemase gene alr is an alternative to antibiotic resistance genes in cloning systems for industrial Corynebacterium glutamicum strains."
    Tauch A., Goetker S., Puehler A., Kalinowski J., Thierbach G.
    J. Biotechnol. 99:79-91(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  2. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
    Ikeda M., Nakagawa S.
    Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  4. "Crystal structure of alanine racemase from Corynebacterium glutamicum at 2.1 A resolution."
    Miyaguchi I., Sasaki C., Kato R., Oikawa T., Sugio S.
    Submitted (SEP-2006) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) IN COMPLEX WITH PYRIDOXAL PHOSPHATE, COFACTOR, SUBUNIT, PYRIDOXAL PHOSPHATE AT LYS-34.

Entry informationi

Entry nameiALR_CORGL
AccessioniPrimary (citable) accession number: Q8RSU9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2002
Last sequence update: June 1, 2002
Last modified: October 29, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3