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Protein

Alanine racemase

Gene

alr

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the interconversion of L-alanine and D-alanine. May also act on other amino acids.UniRule annotation

Catalytic activityi

L-alanine = D-alanine.UniRule annotation

Cofactori

pyridoxal 5'-phosphateUniRule annotation1 Publication

Pathway: D-alanine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes D-alanine from L-alanine.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Alanine racemase (alr)
This subpathway is part of the pathway D-alanine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-alanine from L-alanine, the pathway D-alanine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei34 – 341Proton acceptor; specific for D-alanineUniRule annotation
Binding sitei129 – 1291SubstrateUniRule annotation
Active sitei256 – 2561Proton acceptor; specific for L-alanineUniRule annotation
Binding sitei304 – 3041Substrate; via amide nitrogenUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00042; UER00497.

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine racemaseUniRule annotation (EC:5.1.1.1UniRule annotation)
Gene namesi
Name:alr
Ordered Locus Names:Cgl0588, cg0681
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
ProteomesiUP000000582 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 361361Alanine racemasePRO_0000114512Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei34 – 341N6-(pyridoxal phosphate)lysine

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi196627.cg0681.

Structurei

Secondary structure

1
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 97Combined sources
Helixi10 – 2415Combined sources
Beta strandi27 – 326Combined sources
Helixi34 – 396Combined sources
Helixi42 – 5110Combined sources
Beta strandi56 – 616Combined sources
Helixi62 – 709Combined sources
Beta strandi75 – 795Combined sources
Helixi88 – 925Combined sources
Turni93 – 953Combined sources
Beta strandi97 – 1004Combined sources
Helixi103 – 1108Combined sources
Beta strandi117 – 1237Combined sources
Beta strandi129 – 1324Combined sources
Helixi134 – 14512Combined sources
Beta strandi150 – 1567Combined sources
Helixi170 – 18617Combined sources
Helixi199 – 2046Combined sources
Helixi206 – 2083Combined sources
Helixi217 – 2204Combined sources
Beta strandi236 – 2416Combined sources
Beta strandi244 – 2474Combined sources
Beta strandi267 – 2726Combined sources
Turni275 – 2784Combined sources
Helixi281 – 2833Combined sources
Turni284 – 2863Combined sources
Beta strandi288 – 2914Combined sources
Beta strandi294 – 3007Combined sources
Beta strandi307 – 3126Combined sources
Beta strandi323 – 3286Combined sources
Helixi334 – 3407Combined sources
Helixi345 – 3506Combined sources
Beta strandi357 – 3615Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DY3X-ray2.10A/B/C/D1-361[»]
ProteinModelPortaliQ8RSU9.
SMRiQ8RSU9. Positions 1-361.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8RSU9.

Family & Domainsi

Sequence similaritiesi

Belongs to the alanine racemase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0787.
HOGENOMiHOG000031444.
KOiK01775.
OMAiPSDWVRP.
OrthoDBiEOG6PP9NJ.

Family and domain databases

Gene3Di2.40.37.10. 1 hit.
3.20.20.10. 1 hit.
HAMAPiMF_01201. Ala_racemase.
InterProiIPR000821. Ala_racemase.
IPR009006. Ala_racemase/Decarboxylase_C.
IPR011079. Ala_racemase_C.
IPR001608. Ala_racemase_N.
IPR029066. PLP-binding_barrel.
[Graphical view]
PfamiPF00842. Ala_racemase_C. 1 hit.
PF01168. Ala_racemase_N. 1 hit.
[Graphical view]
PRINTSiPR00992. ALARACEMASE.
SMARTiSM01005. Ala_racemase_C. 1 hit.
[Graphical view]
SUPFAMiSSF50621. SSF50621. 1 hit.
SSF51419. SSF51419. 1 hit.
TIGRFAMsiTIGR00492. alr. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8RSU9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLLTTKIDL DAIAHNTRVL KQMAGPAKLM AVVKANAYNH GVEKVAPVIA
60 70 80 90 100
AHGADAFGVA TLAEAMQLRD IGISQEVLCW IWTPEQDFRA AIDRNIDLAV
110 120 130 140 150
ISPAHAKALI ETDAEHIRVS IKIDSGLHRS GVDEQEWEGV FSALAAAPHI
160 170 180 190 200
EVTGMFTHLA CADEPENPET DRQIIAFRRA LALARKHGLE CPVNHVCNSP
210 220 230 240 250
AFLTRSDLHM EMVRPGLAFY GLEPVAGLEH GLKPAMTWEA KVSVVKQIEA
260 270 280 290 300
GQGTSYGLTW RAEDRGFVAV VPAGYADGMP RHAQGKFSVT IDGLDYPQVG
310 320 330 340 350
RVCMDQFVIS LGDNPHGVEA GAKAVIFGEN GHDATDFAER LDTINYEVVC
360
RPTGRTVRAY V
Length:361
Mass (Da):39,174
Last modified:June 1, 2002 - v1
Checksum:i6E95CAF6456B6037
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY077456 Genomic DNA. Translation: AAL77207.1.
BA000036 Genomic DNA. Translation: BAB97981.1.
BX927149 Genomic DNA. Translation: CAF19293.1.
RefSeqiNP_599824.1. NC_003450.3.
WP_011013748.1. NC_006958.1.
YP_224879.1. NC_006958.1.

Genome annotation databases

EnsemblBacteriaiBAB97981; BAB97981; BAB97981.
CAF19293; CAF19293; cg0681.
GeneIDi1018592.
KEGGicgb:cg0681.
cgl:NCgl0563.
PATRICi21493226. VBICorGlu203724_0579.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY077456 Genomic DNA. Translation: AAL77207.1.
BA000036 Genomic DNA. Translation: BAB97981.1.
BX927149 Genomic DNA. Translation: CAF19293.1.
RefSeqiNP_599824.1. NC_003450.3.
WP_011013748.1. NC_006958.1.
YP_224879.1. NC_006958.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DY3X-ray2.10A/B/C/D1-361[»]
ProteinModelPortaliQ8RSU9.
SMRiQ8RSU9. Positions 1-361.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi196627.cg0681.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB97981; BAB97981; BAB97981.
CAF19293; CAF19293; cg0681.
GeneIDi1018592.
KEGGicgb:cg0681.
cgl:NCgl0563.
PATRICi21493226. VBICorGlu203724_0579.

Phylogenomic databases

eggNOGiCOG0787.
HOGENOMiHOG000031444.
KOiK01775.
OMAiPSDWVRP.
OrthoDBiEOG6PP9NJ.

Enzyme and pathway databases

UniPathwayiUPA00042; UER00497.

Miscellaneous databases

EvolutionaryTraceiQ8RSU9.

Family and domain databases

Gene3Di2.40.37.10. 1 hit.
3.20.20.10. 1 hit.
HAMAPiMF_01201. Ala_racemase.
InterProiIPR000821. Ala_racemase.
IPR009006. Ala_racemase/Decarboxylase_C.
IPR011079. Ala_racemase_C.
IPR001608. Ala_racemase_N.
IPR029066. PLP-binding_barrel.
[Graphical view]
PfamiPF00842. Ala_racemase_C. 1 hit.
PF01168. Ala_racemase_N. 1 hit.
[Graphical view]
PRINTSiPR00992. ALARACEMASE.
SMARTiSM01005. Ala_racemase_C. 1 hit.
[Graphical view]
SUPFAMiSSF50621. SSF50621. 1 hit.
SSF51419. SSF51419. 1 hit.
TIGRFAMsiTIGR00492. alr. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The alanine racemase gene alr is an alternative to antibiotic resistance genes in cloning systems for industrial Corynebacterium glutamicum strains."
    Tauch A., Goetker S., Puehler A., Kalinowski J., Thierbach G.
    J. Biotechnol. 99:79-91(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  2. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
    Ikeda M., Nakagawa S.
    Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  4. "Crystal structure of alanine racemase from Corynebacterium glutamicum at 2.1 A resolution."
    Miyaguchi I., Sasaki C., Kato R., Oikawa T., Sugio S.
    Submitted (SEP-2006) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) IN COMPLEX WITH PYRIDOXAL PHOSPHATE, COFACTOR, SUBUNIT, PYRIDOXAL PHOSPHATE AT LYS-34.

Entry informationi

Entry nameiALR_CORGL
AccessioniPrimary (citable) accession number: Q8RSU9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2002
Last sequence update: June 1, 2002
Last modified: June 24, 2015
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.