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Q8RSU9

- ALR_CORGL

UniProt

Q8RSU9 - ALR_CORGL

Protein

Alanine racemase

Gene

alr

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
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    • Comment

    Functioni

    Catalyzes the interconversion of L-alanine and D-alanine. May also act on other amino acids.UniRule annotation

    Catalytic activityi

    L-alanine = D-alanine.UniRule annotation

    Cofactori

    Pyridoxal phosphate.1 PublicationUniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei34 – 341Proton acceptor; specific for D-alanineUniRule annotation
    Binding sitei129 – 1291SubstrateUniRule annotation
    Active sitei256 – 2561Proton acceptor; specific for L-alanineUniRule annotation
    Binding sitei304 – 3041Substrate; via amide nitrogenUniRule annotation

    GO - Molecular functioni

    1. alanine racemase activity Source: UniProtKB-HAMAP
    2. pyridoxal phosphate binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. D-alanine biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Isomerase

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    UniPathwayiUPA00042; UER00497.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alanine racemaseUniRule annotation (EC:5.1.1.1UniRule annotation)
    Gene namesi
    Name:alr
    Ordered Locus Names:Cgl0588, cg0681
    OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
    Taxonomic identifieri196627 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
    ProteomesiUP000000582: Chromosome, UP000001009: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 361361Alanine racemasePRO_0000114512Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei34 – 341N6-(pyridoxal phosphate)lysine

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    STRINGi196627.cg0681.

    Structurei

    Secondary structure

    1
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 97
    Helixi10 – 2415
    Beta strandi27 – 326
    Helixi34 – 396
    Helixi42 – 5110
    Beta strandi56 – 616
    Helixi62 – 709
    Beta strandi75 – 795
    Helixi88 – 925
    Turni93 – 953
    Beta strandi97 – 1004
    Helixi103 – 1108
    Beta strandi117 – 1237
    Beta strandi129 – 1324
    Helixi134 – 14512
    Beta strandi150 – 1567
    Helixi170 – 18617
    Helixi199 – 2046
    Helixi206 – 2083
    Helixi217 – 2204
    Beta strandi236 – 2416
    Beta strandi244 – 2474
    Beta strandi267 – 2726
    Turni275 – 2784
    Helixi281 – 2833
    Turni284 – 2863
    Beta strandi288 – 2914
    Beta strandi294 – 3007
    Beta strandi307 – 3126
    Beta strandi323 – 3286
    Helixi334 – 3407
    Helixi345 – 3506
    Beta strandi357 – 3615

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2DY3X-ray2.10A/B/C/D1-361[»]
    ProteinModelPortaliQ8RSU9.
    SMRiQ8RSU9. Positions 1-361.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ8RSU9.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the alanine racemase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0787.
    HOGENOMiHOG000031444.
    KOiK01775.
    OMAiPSECAGV.
    OrthoDBiEOG6PP9NJ.

    Family and domain databases

    Gene3Di2.40.37.10. 1 hit.
    3.20.20.10. 1 hit.
    HAMAPiMF_01201. Ala_racemase.
    InterProiIPR000821. Ala_racemase.
    IPR009006. Ala_racemase/Decarboxylase_C.
    IPR011079. Ala_racemase_C.
    IPR001608. Ala_racemase_N.
    IPR029066. PLP-binding_barrel.
    [Graphical view]
    PfamiPF00842. Ala_racemase_C. 1 hit.
    PF01168. Ala_racemase_N. 1 hit.
    [Graphical view]
    PRINTSiPR00992. ALARACEMASE.
    SMARTiSM01005. Ala_racemase_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF50621. SSF50621. 1 hit.
    SSF51419. SSF51419. 1 hit.
    TIGRFAMsiTIGR00492. alr. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8RSU9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNLLTTKIDL DAIAHNTRVL KQMAGPAKLM AVVKANAYNH GVEKVAPVIA    50
    AHGADAFGVA TLAEAMQLRD IGISQEVLCW IWTPEQDFRA AIDRNIDLAV 100
    ISPAHAKALI ETDAEHIRVS IKIDSGLHRS GVDEQEWEGV FSALAAAPHI 150
    EVTGMFTHLA CADEPENPET DRQIIAFRRA LALARKHGLE CPVNHVCNSP 200
    AFLTRSDLHM EMVRPGLAFY GLEPVAGLEH GLKPAMTWEA KVSVVKQIEA 250
    GQGTSYGLTW RAEDRGFVAV VPAGYADGMP RHAQGKFSVT IDGLDYPQVG 300
    RVCMDQFVIS LGDNPHGVEA GAKAVIFGEN GHDATDFAER LDTINYEVVC 350
    RPTGRTVRAY V 361
    Length:361
    Mass (Da):39,174
    Last modified:June 1, 2002 - v1
    Checksum:i6E95CAF6456B6037
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY077456 Genomic DNA. Translation: AAL77207.1.
    BA000036 Genomic DNA. Translation: BAB97981.1.
    BX927149 Genomic DNA. Translation: CAF19293.1.
    RefSeqiNP_599824.1. NC_003450.3.
    WP_011013748.1. NC_006958.1.
    YP_224879.1. NC_006958.1.

    Genome annotation databases

    EnsemblBacteriaiBAB97981; BAB97981; BAB97981.
    CAF19293; CAF19293; cg0681.
    GeneIDi1018592.
    KEGGicgb:cg0681.
    cgl:NCgl0563.
    PATRICi21493226. VBICorGlu203724_0579.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY077456 Genomic DNA. Translation: AAL77207.1 .
    BA000036 Genomic DNA. Translation: BAB97981.1 .
    BX927149 Genomic DNA. Translation: CAF19293.1 .
    RefSeqi NP_599824.1. NC_003450.3.
    WP_011013748.1. NC_006958.1.
    YP_224879.1. NC_006958.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2DY3 X-ray 2.10 A/B/C/D 1-361 [» ]
    ProteinModelPortali Q8RSU9.
    SMRi Q8RSU9. Positions 1-361.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 196627.cg0681.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB97981 ; BAB97981 ; BAB97981 .
    CAF19293 ; CAF19293 ; cg0681 .
    GeneIDi 1018592.
    KEGGi cgb:cg0681.
    cgl:NCgl0563.
    PATRICi 21493226. VBICorGlu203724_0579.

    Phylogenomic databases

    eggNOGi COG0787.
    HOGENOMi HOG000031444.
    KOi K01775.
    OMAi PSECAGV.
    OrthoDBi EOG6PP9NJ.

    Enzyme and pathway databases

    UniPathwayi UPA00042 ; UER00497 .

    Miscellaneous databases

    EvolutionaryTracei Q8RSU9.

    Family and domain databases

    Gene3Di 2.40.37.10. 1 hit.
    3.20.20.10. 1 hit.
    HAMAPi MF_01201. Ala_racemase.
    InterProi IPR000821. Ala_racemase.
    IPR009006. Ala_racemase/Decarboxylase_C.
    IPR011079. Ala_racemase_C.
    IPR001608. Ala_racemase_N.
    IPR029066. PLP-binding_barrel.
    [Graphical view ]
    Pfami PF00842. Ala_racemase_C. 1 hit.
    PF01168. Ala_racemase_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00992. ALARACEMASE.
    SMARTi SM01005. Ala_racemase_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50621. SSF50621. 1 hit.
    SSF51419. SSF51419. 1 hit.
    TIGRFAMsi TIGR00492. alr. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The alanine racemase gene alr is an alternative to antibiotic resistance genes in cloning systems for industrial Corynebacterium glutamicum strains."
      Tauch A., Goetker S., Puehler A., Kalinowski J., Thierbach G.
      J. Biotechnol. 99:79-91(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
    2. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
      Ikeda M., Nakagawa S.
      Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
    4. "Crystal structure of alanine racemase from Corynebacterium glutamicum at 2.1 A resolution."
      Miyaguchi I., Sasaki C., Kato R., Oikawa T., Sugio S.
      Submitted (SEP-2006) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) IN COMPLEX WITH PYRIDOXAL PHOSPHATE, COFACTOR, SUBUNIT, PYRIDOXAL PHOSPHATE AT LYS-34.

    Entry informationi

    Entry nameiALR_CORGL
    AccessioniPrimary (citable) accession number: Q8RSU9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 10, 2002
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3