Reviewed, UniProtKB/Swiss-Prot Q8RSS4 (CARA_HALER)

Names and origin

Protein names

Recommended name:
    Carbamoyl-phosphate synthase small chain
    EC=6.3.5.5
Alternative name(s):
    Carbamoyl-phosphate synthetase glutamine chain

Gene names

Name:

carA

Organism

Halomonas eurihalina

Taxonomic identifier

42566 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Oceanospirillales › Halomonadaceae › Halomonas

Protein attributes

Sequence length	381 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP MF_01209
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1. HAMAP MF_01209 Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3. HAMAP MF_01209
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity.
Sequence similarities	Belongs to the carA family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 381	381	Carbamoyl-phosphate synthase small chain HAMAP MF_01209		PRO_0000112280
Regions
	Domain	196 – 381	186	Glutamine amidotransferase type-1
	Region	1 – 192	192	CPSase HAMAP MF_01209
Sites
	Active site	272	1	Nucleophile By similarity
	Active site	356	1	By similarity
	Active site	358	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8RSS4-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 77F6BC2821917CCB	FASTA	381	41,183
	10 20 30 40 50 60 MSKPAILALE DGSVFHGTAI GADGQTSGEV VFNTAMTGYQ EILTDPSYSR QIVTLTYPHI 70 80 90 100 110 120 GNTGVNSEDV ESSSIAAAGL VIRDLPLLAS SFRCEQSMSD YLAQQNVLGI ADMHTRRLTR 130 140 150 160 170 180 LLRDKGSQNG AILAGPDAEG EDAEARALEA ARAFPGLKGM DLAKVVSCAE PYEWSEGEWT 190 200 210 220 230 240 LGSGYADVSQ GDRPYHVVAY DYGMKRNILR MLAARGCRLT VVPAQTPAEE VLAMNPDGIF 250 260 270 280 290 300 LSNGPGDPEP CDYAISAIQA FLETEIPVFG ICLGHQMLAL ASGAQTVKMN HGHHGANHPV 310 320 330 340 350 360 QDLDSGRVMI TSQNHGFAVE ESTLPDNLRA IHRSLFDGTL QGIERTDRPA FSFQGHPEAS 370 380 PGPRDVAPMF DRFVEMMRHR R

References

[1]

"Identification and characterization of the carAB genes responsible for encoding carbamoylphosphate synthetase in Halomonas eurihalina." Llamas I., Suarez A., Quesada E., Bejar V., del Moral A. Extremophiles 7:205-211(2003) [PubMed: 12768451] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: F2-7.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ431666 Genomic DNA. Translation: CAD24314.1.
3D structure databases
HSSP	HSSP built from PDB template 1M6V based on UniProtKB P00907.
ModBase	Search...
Enzyme and pathway databases
BRENDA	6.3.5.5. 288616.
Family and domain databases
HAMAP	MF_01209. [Tree]
InterPro	IPR001317. CarbamoylP_synth_GATase. IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11405:SF4. CarA_synth_small. 1 hit.
Pfam	PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
PRINTS	PR00099. CPSGATASE. PR00096. GATASE.
TIGRFAMs	TIGR01368. CPSaseIIsmall. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARA_HALER

Accession

Primary (citable) accession number: Q8RSS4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2002
Last sequence update:	June 1, 2002
Last modified:	September 22, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)