Q8RR57 (SSRP_THET8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
SsrA-binding protein

Gene names

Name:	smpB
Ordered Locus Names:	TTHA0902

Organism

Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Reference proteome] [HAMAP]

Taxonomic identifier

300852 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus › Thermus thermophilus

Protein attributes

Sequence length	144 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds specifically to the SsrA RNA (tmRNA) and is required for stable association of SsrA with ribosomes By similarity. HAMAP-Rule MF_00023
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00023.
Sequence similarities	Belongs to the SmpB family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	RNA-binding
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	RNA binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 144

144

SsrA-binding protein HAMAP-Rule MF_00023

PRO_0000103056

Secondary structure

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144

Helix Strand Turn

Details...

Helix

8 – 13

6

Beta strand

14 – 24

11

Helix

30 – 35

6

Beta strand

45 – 49

5

Beta strand

52 – 57

6

Beta strand

60 – 63

4

Turn

65 – 68

4

Beta strand

77 – 80

4

Helix

83 – 89

7

Turn

90 – 92

3

Turn

94 – 97

4

Beta strand

98 – 107

10

Turn

109 – 111

3

Beta strand

113 – 122

10

Helix

133 – 143

11

Sequences

	Sequence		Length	Mass (Da)
	Q8RR57 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 4691B4D48F1A2299	FASTA	144	16,750
	10 20 30 40 50 60 MAPVLENRRA RHDYEILETY EAGIALKGTE VKSLRAGKVD FTGSFARFED GELYLENLYI 70 80 90 100 110 120 APYEKGSYAN VDPRRKRKLL LHKHELRRLL GKVEQKGLTL VPLKIYFNER GYAKVLLGLA 130 140 RGKKAYEKRR EDKKEAVRRA LEEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Interaction of tmRNA with a tmRNA-binding protein, SmpB, from Thermus thermophilus." Nameki N., Kimoto M., Terada T., Shirouzu M., Suetsugu-Hanawa K., Takaku H., Himeno H., Muto A., Inoue Y., Shibata T., Kuramitsu S., Yokoyama S., Kawai G. Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete genome sequence of Thermus thermophilus HB8." Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S. Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HB8 / ATCC 27634 / DSM 579.
[3]	"Crystal structure of the tRNA domain of tmRNA from Thermus thermophilus HB8." RIKEN structural genomics initiative (RSGI) Submitted (OCT-2006) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (3.01 ANGSTROMS) OF 1-123.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB070601 Genomic DNA. Translation: BAB86918.1.
AP008226 Genomic DNA. Translation: BAD70725.1.

RefSeq

YP_144168.1. NC_006461.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1J1H	NMR	-	A	1-144	[»]
1WJX	X-ray	1.70	A	2-123	[»]
2CZJ	X-ray	3.01	A/C/E/G	1-123	[»]
3IYQ	electron microscopy	-	B	2-144	[»]
3IYR	electron microscopy	-	B	2-144	[»]
3IZ4	electron microscopy	-	B	2-123	[»]
4ABR	X-ray	3.10	2	1-144	[»]

ProteinModelPortal

Q8RR57.

SMR

Q8RR57. Positions 1-123.

ModBase

Search...

Protein-protein interaction databases

STRING

300852.TTHA0902.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

BAD70725; BAD70725; BAD70725.

GeneID

3169917.

KEGG

ttj:TTHA0902.

PATRIC

23956784. VBITheThe93045_0894.

Phylogenomic databases

eggNOG

COG0691.

HOGENOM

HOG000009628.

KO

K03664.

OMA

HVSALNS.

ProtClustDB

PRK05422.

Family and domain databases

Gene3D

2.40.280.10. 1 hit.

HAMAP

MF_00023. SmpB.

InterPro

IPR023620. SmpB.
IPR000037. SsrA-bd_prot.
IPR020081. SsrA-bd_prot_CS.
[Graphical view]

Pfam

PF01668. SmpB. 1 hit.
[Graphical view]

ProDom

PD004488. SmpB. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SUPFAM

SSF74982. SmpB. 1 hit.

TIGRFAMs

TIGR00086. smpB. 1 hit.

PROSITE

PS01317. SSRP. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q8RR57.

Entry information

Entry name

SSRP_THET8

Accession

Primary (citable) accession number: Q8RR57
Secondary accession number(s): Q5SMG1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 23, 2003
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program