Reviewed, UniProtKB/Swiss-Prot Q8RQP4 (DHE4_COREF)

Names and origin

Protein names

Recommended name:
    NADP-specific glutamate dehydrogenase
      Short name=NADP-GDH
    EC=1.4.1.4

Gene names

Name:	gdh
Ordered Locus Names:	CE1982

Organism

Corynebacterium efficiens [Complete proteome] [HAMAP]

Taxonomic identifier

152794 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	447 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.
Subunit structure	Homohexamer By similarity.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	amino acid metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro glutamate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 447	447	NADP-specific glutamate dehydrogenase		PRO_0000182767
Sites
	Active site	128	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8RQP4-1 [UniParc]. Last modified November 1, 2002. Version 2. Checksum: B2B320AAE3EA70A3	FASTA	447	48,962
	10 20 30 40 50 60 MTVDEQVSNY YDMLLKRNAG EPEFHQAVAE VLESLKIVLE KDPHYADYGL IQRLCEPERQ 70 80 90 100 110 120 LIFRVPWVDD NGQVHVNRGF RVQFNSALGP YKGGLRFHPS VNLGIVKFLG FEQIFKNSLT 130 140 150 160 170 180 GLPIGGGKGG SDFDPKGKSE LEIMRFCQSF MTELHRHIGE YRDVPAGDIG VGGREIGYLF 190 200 210 220 230 240 GHYRRLANQH ESGVLTGKGL TWGGSLVRTE ATGFGTVYFV QEMIKAEGET LEGKKVIVSG 250 260 270 280 290 300 SGNVATYAIQ KVQELGAVVV GFSDSSGWVS TPNGVDVAKL REIKEVRRAR VSSYADEVEG 310 320 330 340 350 360 AEYHTDGSIW DLTADIALPC ATQNELDGDN ARTLADNGCR FVAEGANMPS TPEAIDVFRE 370 380 390 400 410 420 RGVLFGPGKA ANAGGVATSA LEMQQNASRD SWSFEYTDER LHRIMKNIFK SCADTAKEYG 430 440 HEKNYVVGAN IAGFKKVADA MLAQGVI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Corynebacterium efficiens gdh gene encoding glutamate dehydrogenase NADP dependent." Matsuzaki Y., Kimura E., Nakamura K., Kawahara Y., Sugimoto S. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.
[2]	"Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens." Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T. Genome Res. 13:1572-1579(2003) [PubMed: 12840036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB082375 Genomic DNA. Translation: BAB86838.1. BA000035 Genomic DNA. Translation: BAC18792.1. Different initiation.
RefSeq	NP_738592.1.
3D structure databases
HSSP	HSSP built from PDB template 1AUP based on UniProtKB P24295.
ModBase	Search...
Genome annotation databases
GeneID	1032052.
GenomeReviews	Gene locus CE1982 in contig BA000035_GR.
KEGG	cef:CE1982.
NMPDR	fig|196164.1.peg.1982.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8RQP4.
Enzyme and pathway databases
BioCyc	CEFF196164:CE1982-MON.
BRENDA	1.4.1.4. 277326.
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DH. IPR006096. Glu/Leu/Phe/Val_DH_C. IPR006097. Glu/Leu/Phe/Val_DH_dimer. IPR014362. Glu_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11606:SF2. GLFV_DH. 1 hit.
Pfam	PF00208. ELFV_dehydrog. 1 hit. PF02812. ELFV_dehydrog_N. 1 hit. [Graphical view]
PIRSF	PIRSF000185. Glu_DH. 1 hit.
PRINTS	PR00082. GLFDHDRGNASE.
PROSITE	PS00074. GLFV_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHE4_COREF

Accession

Primary (citable) accession number: Q8RQP4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 2002
Last sequence update:	November 1, 2002
Last modified:	June 16, 2009
This is version 42 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)