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Q8RQP4

- DHE4_COREF

UniProt

Q8RQP4 - DHE4_COREF

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Protein
NADP-specific glutamate dehydrogenase
Gene
gdh, CE1982
Organism
Corynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the reversible oxidative deamination of glutamate to alpha-ketoglutarate and ammonia By similarity.

Catalytic activityi

L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei92 – 921Substrate By similarity
Binding sitei113 – 1131Substrate By similarity
Binding sitei116 – 1161Substrate By similarity
Active sitei128 – 1281Proton donor By similarity
Binding sitei167 – 1671Substrate; via carbonyl oxygen By similarity
Sitei168 – 1681Important for catalysis By similarity
Binding sitei212 – 2121NADP By similarity
Binding sitei243 – 2431NADP By similarity
Binding sitei379 – 3791Substrate By similarity

GO - Molecular functioni

  1. glutamate dehydrogenase (NADP+) activity Source: UniProtKB

GO - Biological processi

  1. glutamate biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciCEFF196164:GJW8-2018-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NADP-specific glutamate dehydrogenase (EC:1.4.1.4)
Short name:
NADP-GDH
Gene namesi
Name:gdh
Ordered Locus Names:CE1982
OrganismiCorynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
Taxonomic identifieri196164 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
ProteomesiUP000001409: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 447447NADP-specific glutamate dehydrogenase
PRO_0000182767Add
BLAST

Proteomic databases

PRIDEiQ8RQP4.

Interactioni

Subunit structurei

Homohexamer By similarity.

Protein-protein interaction databases

STRINGi196164.CE1982.

Structurei

3D structure databases

ProteinModelPortaliQ8RQP4.
SMRiQ8RQP4. Positions 3-446.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000243799.
KOiK00262.
OrthoDBiEOG65XN4D.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR006095. Glu/Leu/Phe/Val_DH.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000185. Glu_DH. 1 hit.
PRINTSiPR00082. GLFDHDRGNASE.
SMARTiSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8RQP4-1 [UniParc]FASTAAdd to Basket

« Hide

MTVDEQVSNY YDMLLKRNAG EPEFHQAVAE VLESLKIVLE KDPHYADYGL    50
IQRLCEPERQ LIFRVPWVDD NGQVHVNRGF RVQFNSALGP YKGGLRFHPS 100
VNLGIVKFLG FEQIFKNSLT GLPIGGGKGG SDFDPKGKSE LEIMRFCQSF 150
MTELHRHIGE YRDVPAGDIG VGGREIGYLF GHYRRLANQH ESGVLTGKGL 200
TWGGSLVRTE ATGFGTVYFV QEMIKAEGET LEGKKVIVSG SGNVATYAIQ 250
KVQELGAVVV GFSDSSGWVS TPNGVDVAKL REIKEVRRAR VSSYADEVEG 300
AEYHTDGSIW DLTADIALPC ATQNELDGDN ARTLADNGCR FVAEGANMPS 350
TPEAIDVFRE RGVLFGPGKA ANAGGVATSA LEMQQNASRD SWSFEYTDER 400
LHRIMKNIFK SCADTAKEYG HEKNYVVGAN IAGFKKVADA MLAQGVI 447
Length:447
Mass (Da):48,962
Last modified:November 1, 2002 - v2
Checksum:iB2B320AAE3EA70A3
GO

Sequence cautioni

The sequence BAC18792.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB082375 Genomic DNA. Translation: BAB86838.1.
BA000035 Genomic DNA. Translation: BAC18792.1. Different initiation.
RefSeqiNP_738592.1. NC_004369.1.

Genome annotation databases

EnsemblBacteriaiBAC18792; BAC18792; BAC18792.
GeneIDi1032052.
KEGGicef:CE1982.
PATRICi21490097. VBICorEff9312_1963.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB082375 Genomic DNA. Translation: BAB86838.1 .
BA000035 Genomic DNA. Translation: BAC18792.1 . Different initiation.
RefSeqi NP_738592.1. NC_004369.1.

3D structure databases

ProteinModelPortali Q8RQP4.
SMRi Q8RQP4. Positions 3-446.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 196164.CE1982.

Proteomic databases

PRIDEi Q8RQP4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAC18792 ; BAC18792 ; BAC18792 .
GeneIDi 1032052.
KEGGi cef:CE1982.
PATRICi 21490097. VBICorEff9312_1963.

Phylogenomic databases

HOGENOMi HOG000243799.
KOi K00262.
OrthoDBi EOG65XN4D.

Enzyme and pathway databases

BioCyci CEFF196164:GJW8-2018-MONOMER.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR006095. Glu/Leu/Phe/Val_DH.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000185. Glu_DH. 1 hit.
PRINTSi PR00082. GLFDHDRGNASE.
SMARTi SM00839. ELFV_dehydrog. 1 hit.
[Graphical view ]
PROSITEi PS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Corynebacterium efficiens gdh gene encoding glutamate dehydrogenase NADP dependent."
    Matsuzaki Y., Kimura E., Nakamura K., Kawahara Y., Sugimoto S.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.
  2. "Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens."
    Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T.
    Genome Res. 13:1572-1579(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Entry informationi

Entry nameiDHE4_COREF
AccessioniPrimary (citable) accession number: Q8RQP4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 2002
Last sequence update: November 1, 2002
Last modified: May 14, 2014
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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