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Q8RQP4

- DHE4_COREF

UniProt

Q8RQP4 - DHE4_COREF

Protein

NADP-specific glutamate dehydrogenase

Gene

gdh

Organism
Corynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 2 (01 Nov 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible oxidative deamination of glutamate to alpha-ketoglutarate and ammonia.By similarity

    Catalytic activityi

    L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei92 – 921SubstrateBy similarity
    Binding sitei113 – 1131SubstrateBy similarity
    Binding sitei116 – 1161SubstrateBy similarity
    Active sitei128 – 1281Proton donorPROSITE-ProRule annotation
    Binding sitei167 – 1671Substrate; via carbonyl oxygenBy similarity
    Sitei168 – 1681Important for catalysisBy similarity
    Binding sitei212 – 2121NADPBy similarity
    Binding sitei243 – 2431NADPBy similarity
    Binding sitei379 – 3791SubstrateBy similarity

    GO - Molecular functioni

    1. glutamate dehydrogenase (NADP+) activity Source: UniProtKB

    GO - Biological processi

    1. glutamate biosynthetic process Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    BioCyciCEFF196164:GJW8-2018-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADP-specific glutamate dehydrogenase (EC:1.4.1.4)
    Short name:
    NADP-GDH
    Gene namesi
    Name:gdh
    Ordered Locus Names:CE1982
    OrganismiCorynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
    Taxonomic identifieri196164 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
    ProteomesiUP000001409: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 447447NADP-specific glutamate dehydrogenasePRO_0000182767Add
    BLAST

    Proteomic databases

    PRIDEiQ8RQP4.

    Interactioni

    Subunit structurei

    Homohexamer.By similarity

    Protein-protein interaction databases

    STRINGi196164.CE1982.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8RQP4.
    SMRiQ8RQP4. Positions 3-446.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    HOGENOMiHOG000243799.
    KOiK00262.
    OrthoDBiEOG65XN4D.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR006095. Glu/Leu/Phe/Val_DH.
    IPR006096. Glu/Leu/Phe/Val_DH_C.
    IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
    IPR014362. Glu_DH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF00208. ELFV_dehydrog. 1 hit.
    PF02812. ELFV_dehydrog_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000185. Glu_DH. 1 hit.
    PRINTSiPR00082. GLFDHDRGNASE.
    SMARTiSM00839. ELFV_dehydrog. 1 hit.
    [Graphical view]
    PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8RQP4-1 [UniParc]FASTAAdd to Basket

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    MTVDEQVSNY YDMLLKRNAG EPEFHQAVAE VLESLKIVLE KDPHYADYGL    50
    IQRLCEPERQ LIFRVPWVDD NGQVHVNRGF RVQFNSALGP YKGGLRFHPS 100
    VNLGIVKFLG FEQIFKNSLT GLPIGGGKGG SDFDPKGKSE LEIMRFCQSF 150
    MTELHRHIGE YRDVPAGDIG VGGREIGYLF GHYRRLANQH ESGVLTGKGL 200
    TWGGSLVRTE ATGFGTVYFV QEMIKAEGET LEGKKVIVSG SGNVATYAIQ 250
    KVQELGAVVV GFSDSSGWVS TPNGVDVAKL REIKEVRRAR VSSYADEVEG 300
    AEYHTDGSIW DLTADIALPC ATQNELDGDN ARTLADNGCR FVAEGANMPS 350
    TPEAIDVFRE RGVLFGPGKA ANAGGVATSA LEMQQNASRD SWSFEYTDER 400
    LHRIMKNIFK SCADTAKEYG HEKNYVVGAN IAGFKKVADA MLAQGVI 447
    Length:447
    Mass (Da):48,962
    Last modified:November 1, 2002 - v2
    Checksum:iB2B320AAE3EA70A3
    GO

    Sequence cautioni

    The sequence BAC18792.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB082375 Genomic DNA. Translation: BAB86838.1.
    BA000035 Genomic DNA. Translation: BAC18792.1. Different initiation.
    RefSeqiNP_738592.1. NC_004369.1.

    Genome annotation databases

    EnsemblBacteriaiBAC18792; BAC18792; BAC18792.
    GeneIDi1032052.
    KEGGicef:CE1982.
    PATRICi21490097. VBICorEff9312_1963.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB082375 Genomic DNA. Translation: BAB86838.1 .
    BA000035 Genomic DNA. Translation: BAC18792.1 . Different initiation.
    RefSeqi NP_738592.1. NC_004369.1.

    3D structure databases

    ProteinModelPortali Q8RQP4.
    SMRi Q8RQP4. Positions 3-446.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 196164.CE1982.

    Proteomic databases

    PRIDEi Q8RQP4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAC18792 ; BAC18792 ; BAC18792 .
    GeneIDi 1032052.
    KEGGi cef:CE1982.
    PATRICi 21490097. VBICorEff9312_1963.

    Phylogenomic databases

    HOGENOMi HOG000243799.
    KOi K00262.
    OrthoDBi EOG65XN4D.

    Enzyme and pathway databases

    BioCyci CEFF196164:GJW8-2018-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR006095. Glu/Leu/Phe/Val_DH.
    IPR006096. Glu/Leu/Phe/Val_DH_C.
    IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
    IPR014362. Glu_DH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF00208. ELFV_dehydrog. 1 hit.
    PF02812. ELFV_dehydrog_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000185. Glu_DH. 1 hit.
    PRINTSi PR00082. GLFDHDRGNASE.
    SMARTi SM00839. ELFV_dehydrog. 1 hit.
    [Graphical view ]
    PROSITEi PS00074. GLFV_DEHYDROGENASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Corynebacterium efficiens gdh gene encoding glutamate dehydrogenase NADP dependent."
      Matsuzaki Y., Kimura E., Nakamura K., Kawahara Y., Sugimoto S.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.
    2. "Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens."
      Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T.
      Genome Res. 13:1572-1579(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

    Entry informationi

    Entry nameiDHE4_COREF
    AccessioniPrimary (citable) accession number: Q8RQP4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 2002
    Last sequence update: November 1, 2002
    Last modified: October 1, 2014
    This is version 74 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3