Q8RQN6 (ACEA_COREF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isocitrate lyase
Short name=ICL
Short name=Isocitrase
Short name=Isocitratase
EC=4.1.3.1

Gene names

Name:	aceA
Ordered Locus Names:	CE2232

Organism

Corynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395) [Complete proteome] [HAMAP]

Taxonomic identifier

196164 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium › Corynebacterium efficiens

Protein attributes

Sequence length	431 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle. May be involved in the assimilation of one-carbon compounds via the isocitrate lyase-positive serine pathway By similarity.
Catalytic activity	Isocitrate = succinate + glyoxylate.
Cofactor	Divalent cations. Magnesium, manganese and cobalt can be used By similarity.
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 1/2.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-UniPathway tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	isocitrate lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 431	430	Isocitrate lyase		PRO_0000068772
Sites
	Active site	193	1	Probable
Experimental info
	Sequence conflict	428	1	G → C in BAB88666. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q8RQN6 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: A6ACDA9530A6B207	FASTA	431	47,031
	10 20 30 40 50 60 MSNVGTPRTA QEIQQDWDTN PRWNGITRDY TAEQVAELQG SVVEEHTLAK RGAEILWDAV 70 80 90 100 110 120 SAEGDDYINA LGALTGNQAV QQVRAGLKAV YLSGWQVAGD ANLAGHTYPD QSLYPANSVP 130 140 150 160 170 180 NVVRRINNAL LRADEIARVE GDTSVDNWLV PIVADGEAGF GGALNVYELQ KGMITAGAAG 190 200 210 220 230 240 THWEDQLASE KKCGHLGGKV LIPTQQHIRT LNSARLAADV ANTPTVVIAR TDAEAATLIT 250 260 270 280 290 300 SDVDERDRPF ITGERTAEGY YHVKPGLEPC IARAKSYAPY ADMIWMETGT PDLELAKKFA 310 320 330 340 350 360 EGVRSEFPDQ LLSYNCSPSF NWSAHLEADE IAKFQKELGA MGFKFQFITL AGFHSLNYGM 370 380 390 400 410 420 FDLAYGYARE GMPAFVDLQN REFKAAEERG FTAVKHQREV GAGYFDTIAT TVDPNSSTTA 430 LKGSTEEGQF H

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"aceA of Corynebacterium efficiens." Hirano S., Kimura E., Kawahara Y., Sugimoto S. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.
[2]	"Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens." Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T. Genome Res. 13:1572-1579(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB083050 Genomic DNA. Translation: BAB88666.1. BA000035 Genomic DNA. Translation: BAC19042.1.
RefSeq	NP_738842.1. NC_004369.1.
3D structure databases
ProteinModelPortal	Q8RQN6.
SMR	Q8RQN6. Positions 2-430.
ModBase	Search...
Protein-protein interaction databases
STRING	196164.CE2232.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAC19042; BAC19042; BAC19042.
GeneID	1032083.
KEGG	cef:CE2232.
PATRIC	21490593. VBICorEff9312_2208.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000238475.
KO	K01637.
OMA	QAVQQVK.
ProtClustDB	PRK15063.
Enzyme and pathway databases
UniPathway	UPA00703; UER00719.
Family and domain databases
Gene3D	3.20.20.60. 1 hit.
InterPro	IPR006254. Isocitrate_lyase. IPR000918. Isocitrate_lyase/Pmutase. IPR018523. Isocitrate_lyase_ph_CS. IPR015813. Pyrv/PenolPyrv_Kinase. [Graphical view]
PANTHER	PTHR21631:SF3. PTHR21631:SF3. 1 hit.
Pfam	PF00463. ICL. 1 hit. [Graphical view]
PIRSF	PIRSF001362. Isocit_lyase. 1 hit.
SUPFAM	SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMs	TIGR01346. isocit_lyase. 2 hits.
PROSITE	PS00161. ISOCITRATE_LYASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACEA_COREF

Accession

Primary (citable) accession number: Q8RQN6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 2002
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 78 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program