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Q8RQN5

- PFKA_COREF

UniProt

Q8RQN5 - PFKA_COREF

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Protein

ATP-dependent 6-phosphofructokinase

Gene
pfkA, pfk, CE1348
Organism
Corynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei13 – 131ATP; via amide nitrogen By similarity
Metal bindingi107 – 1071Magnesium; catalytic By similarity
Sitei108 – 1081Important for substrate specificity; cannot use PPi as phosphoryl donor By similarity
Active sitei131 – 1311Proton acceptor By similarity
Binding sitei166 – 1661Substrate; shared with dimeric partner By similarity
Binding sitei226 – 2261Substrate By similarity
Binding sitei270 – 2701Substrate; shared with dimeric partner By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi76 – 772ATP By similarity
Nucleotide bindingi106 – 1094ATP By similarity

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase (EC:2.7.1.11)
Short name:
ATP-PFK
Short name:
Phosphofructokinase
Alternative name(s):
Phosphohexokinase
Gene namesi
Name:pfkA
Synonyms:pfk
Ordered Locus Names:CE1348
OrganismiCorynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
Taxonomic identifieri196164 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
ProteomesiUP000001409: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 346346ATP-dependent 6-phosphofructokinaseUniRule annotationPRO_0000111947Add
BLAST

Interactioni

Subunit structurei

Homodimer or homotetramer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi196164.CE1348.

Structurei

3D structure databases

ProteinModelPortaliQ8RQN5.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni129 – 1313Substrate binding By similarity
Regioni173 – 1753Substrate binding By similarity
Regioni276 – 2794Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000248869.
KOiK00850.
OMAiISHRIAT.
OrthoDBiEOG644ZRM.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III.
InterProiIPR012003. ATP_PFK_prok.
IPR012829. PFK.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02483. PFK_mixed. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8RQN5-1 [UniParc]FASTAAdd to Basket

« Hide

MGAMRIATLT SGGDCPGLNA VIRGIVRTAS NEFGSTVVGY QDGWEGLLAD    50
RRVQLYDDED IDRILLRGGT ILGTGRLHPD KFRAGIDQVK ANLADAGIDA 100
LIPIGGEGTL KGAKWLADNG IPVVGVPKTI DNDVNGTDFT FGFDSAVSVA 150
TDAIDRLHTT AESHNRVMIV EVMGRHVGWI ALHAGMAGGA HYTVIPEVPF 200
DISEICKRME RRFQMGEKYG IIVVAEGALP KEGTMELREG EVDQFGHKTF 250
TGIGQQIADE VHRRLGHDVR TTVLGHIQRG GTPTAFDRVL ATRYGVRAAR 300
ACHEGQFNTV VALKGERIRM ISFDEAVGTL KKVPMERWVT AQAMFG 346
Length:346
Mass (Da):37,476
Last modified:June 1, 2002 - v1
Checksum:i0F233545F77922A7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB083051 Genomic DNA. Translation: BAB88667.1.
BA000035 Genomic DNA. Translation: BAC18158.1.
RefSeqiNP_737958.1. NC_004369.1.
WP_006769312.1. NZ_GG700686.1.

Genome annotation databases

EnsemblBacteriaiBAC18158; BAC18158; BAC18158.
GeneIDi1033713.
KEGGicef:CE1348.
PATRICi21488828. VBICorEff9312_1347.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB083051 Genomic DNA. Translation: BAB88667.1 .
BA000035 Genomic DNA. Translation: BAC18158.1 .
RefSeqi NP_737958.1. NC_004369.1.
WP_006769312.1. NZ_GG700686.1.

3D structure databases

ProteinModelPortali Q8RQN5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 196164.CE1348.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAC18158 ; BAC18158 ; BAC18158 .
GeneIDi 1033713.
KEGGi cef:CE1348.
PATRICi 21488828. VBICorEff9312_1347.

Phylogenomic databases

HOGENOMi HOG000248869.
KOi K00850.
OMAi ISHRIAT.
OrthoDBi EOG644ZRM.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .

Family and domain databases

HAMAPi MF_01976. Phosphofructokinase_III.
InterProi IPR012003. ATP_PFK_prok.
IPR012829. PFK.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 1 hit.
TIGRFAMsi TIGR02483. PFK_mixed. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "pfk of Corynebacterium efficiens."
    Hirano S., Kimura E., Kawahara Y., Sugimoto S.
    Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.
  2. "Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens."
    Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T.
    Genome Res. 13:1572-1579(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Entry informationi

Entry nameiPFKA_COREF
AccessioniPrimary (citable) accession number: Q8RQN5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 2002
Last sequence update: June 1, 2002
Last modified: September 3, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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