Q8RQN5 (K6PF_COREF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6-phosphofructokinase
Short name=Phosphofructokinase
EC=2.7.1.11

Alternative name(s):
Phosphohexokinase

Gene names

Name:	pfkA
Synonyms:	pfk
Ordered Locus Names:	CE1348

Organism

Corynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395) [Complete proteome] [HAMAP]

Taxonomic identifier

196164 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium › Corynebacterium efficiens

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP-Rule MF_00339
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP-Rule MF_00339
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the phosphofructokinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Allosteric enzyme Complete proteome
Gene Ontology (GO)
Biological_process	fructose 6-phosphate metabolic process Inferred from electronic annotation. Source: InterPro glycolysis Inferred from electronic annotation. Source: HAMAP
Cellular_component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	6-phosphofructokinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	6-phosphofructokinase HAMAP-Rule MF_00339		PRO_0000111947
Regions
	Nucleotide binding	23 – 27	5	ATP By similarity
	Nucleotide binding	158 – 162	5	ATP By similarity
	Nucleotide binding	175 – 191	17	ATP By similarity
Sites
	Active site	131	1	Proton acceptor By similarity
	Metal binding	189	1	Magnesium; via carbonyl oxygen By similarity
	Binding site	166	1	Substrate By similarity
	Binding site	270	1	Substrate By similarity
	Binding site	276	1	Substrate By similarity
	Binding site	279	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8RQN5 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 0F233545F77922A7	FASTA	346	37,476
	10 20 30 40 50 60 MGAMRIATLT SGGDCPGLNA VIRGIVRTAS NEFGSTVVGY QDGWEGLLAD RRVQLYDDED 70 80 90 100 110 120 IDRILLRGGT ILGTGRLHPD KFRAGIDQVK ANLADAGIDA LIPIGGEGTL KGAKWLADNG 130 140 150 160 170 180 IPVVGVPKTI DNDVNGTDFT FGFDSAVSVA TDAIDRLHTT AESHNRVMIV EVMGRHVGWI 190 200 210 220 230 240 ALHAGMAGGA HYTVIPEVPF DISEICKRME RRFQMGEKYG IIVVAEGALP KEGTMELREG 250 260 270 280 290 300 EVDQFGHKTF TGIGQQIADE VHRRLGHDVR TTVLGHIQRG GTPTAFDRVL ATRYGVRAAR 310 320 330 340 ACHEGQFNTV VALKGERIRM ISFDEAVGTL KKVPMERWVT AQAMFG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"pfk of Corynebacterium efficiens." Hirano S., Kimura E., Kawahara Y., Sugimoto S. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.
[2]	"Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens." Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T. Genome Res. 13:1572-1579(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB083051 Genomic DNA. Translation: BAB88667.1. BA000035 Genomic DNA. Translation: BAC18158.1.
RefSeq	NP_737958.1. NC_004369.1.
3D structure databases
ProteinModelPortal	Q8RQN5.
ModBase	Search...
Protein-protein interaction databases
STRING	196164.CE1348.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAC18158; BAC18158; BAC18158.
GeneID	1033713.
KEGG	cef:CE1348.
PATRIC	21488828. VBICorEff9312_1347.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000248869.
KO	K00850.
OMA	DRRVQLY.
ProtClustDB	PRK03202.
Enzyme and pathway databases
UniPathway	UPA00109; UER00182.
Family and domain databases
HAMAP	MF_00339. Phosphofructokinase.
InterPro	IPR012003. ATP_PFK_prok. IPR012829. PFK. IPR022953. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. IPR000023. Phosphofructokinase_dom. [Graphical view]
Pfam	PF00365. PFK. 1 hit. [Graphical view]
PIRSF	PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
SUPFAM	SSF53784. Ppfruckinase. 1 hit.
TIGRFAMs	TIGR02483. PFK_mixed. 1 hit.
PROSITE	PS00433. PHOSPHOFRUCTOKINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

K6PF_COREF

Accession

Primary (citable) accession number: Q8RQN5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 2002
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program