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Q8RQN5

- PFKA_COREF

UniProt

Q8RQN5 - PFKA_COREF

Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Corynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei13 – 131ATP; via amide nitrogenUniRule annotation
    Metal bindingi107 – 1071Magnesium; catalyticUniRule annotation
    Sitei108 – 1081Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation
    Active sitei131 – 1311Proton acceptorUniRule annotation
    Binding sitei166 – 1661Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei226 – 2261SubstrateUniRule annotation
    Binding sitei270 – 2701Substrate; shared with dimeric partnerUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi76 – 772ATPUniRule annotation
    Nucleotide bindingi106 – 1094ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: UniProtKB-EC
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. fructose 6-phosphate metabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
    Short name:
    ATP-PFKUniRule annotation
    Short name:
    PhosphofructokinaseUniRule annotation
    Alternative name(s):
    PhosphohexokinaseUniRule annotation
    Gene namesi
    Name:pfkAUniRule annotation
    Synonyms:pfk
    Ordered Locus Names:CE1348
    OrganismiCorynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
    Taxonomic identifieri196164 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
    ProteomesiUP000001409: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 346346ATP-dependent 6-phosphofructokinasePRO_0000111947Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer or homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi196164.CE1348.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8RQN5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni129 – 1313Substrate bindingUniRule annotation
    Regioni173 – 1753Substrate bindingUniRule annotation
    Regioni276 – 2794Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000248869.
    KOiK00850.
    OMAiISHRIAT.
    OrthoDBiEOG644ZRM.

    Family and domain databases

    HAMAPiMF_01976. Phosphofructokinase_III.
    InterProiIPR012003. ATP_PFK_prok.
    IPR012829. PFK.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 1 hit.
    TIGRFAMsiTIGR02483. PFK_mixed. 1 hit.
    PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8RQN5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGAMRIATLT SGGDCPGLNA VIRGIVRTAS NEFGSTVVGY QDGWEGLLAD    50
    RRVQLYDDED IDRILLRGGT ILGTGRLHPD KFRAGIDQVK ANLADAGIDA 100
    LIPIGGEGTL KGAKWLADNG IPVVGVPKTI DNDVNGTDFT FGFDSAVSVA 150
    TDAIDRLHTT AESHNRVMIV EVMGRHVGWI ALHAGMAGGA HYTVIPEVPF 200
    DISEICKRME RRFQMGEKYG IIVVAEGALP KEGTMELREG EVDQFGHKTF 250
    TGIGQQIADE VHRRLGHDVR TTVLGHIQRG GTPTAFDRVL ATRYGVRAAR 300
    ACHEGQFNTV VALKGERIRM ISFDEAVGTL KKVPMERWVT AQAMFG 346
    Length:346
    Mass (Da):37,476
    Last modified:June 1, 2002 - v1
    Checksum:i0F233545F77922A7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB083051 Genomic DNA. Translation: BAB88667.1.
    BA000035 Genomic DNA. Translation: BAC18158.1.
    RefSeqiNP_737958.1. NC_004369.1.
    WP_006769312.1. NZ_GG700686.1.

    Genome annotation databases

    EnsemblBacteriaiBAC18158; BAC18158; BAC18158.
    GeneIDi1033713.
    KEGGicef:CE1348.
    PATRICi21488828. VBICorEff9312_1347.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB083051 Genomic DNA. Translation: BAB88667.1 .
    BA000035 Genomic DNA. Translation: BAC18158.1 .
    RefSeqi NP_737958.1. NC_004369.1.
    WP_006769312.1. NZ_GG700686.1.

    3D structure databases

    ProteinModelPortali Q8RQN5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 196164.CE1348.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAC18158 ; BAC18158 ; BAC18158 .
    GeneIDi 1033713.
    KEGGi cef:CE1348.
    PATRICi 21488828. VBICorEff9312_1347.

    Phylogenomic databases

    HOGENOMi HOG000248869.
    KOi K00850.
    OMAi ISHRIAT.
    OrthoDBi EOG644ZRM.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .

    Family and domain databases

    HAMAPi MF_01976. Phosphofructokinase_III.
    InterProi IPR012003. ATP_PFK_prok.
    IPR012829. PFK.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 1 hit.
    TIGRFAMsi TIGR02483. PFK_mixed. 1 hit.
    PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "pfk of Corynebacterium efficiens."
      Hirano S., Kimura E., Kawahara Y., Sugimoto S.
      Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.
    2. "Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens."
      Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T.
      Genome Res. 13:1572-1579(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

    Entry informationi

    Entry nameiPFKA_COREF
    AccessioniPrimary (citable) accession number: Q8RQN5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 2002
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3