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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Corynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi)
  3. ATP-dependent 6-phosphofructokinase (pfkA)
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei13ATP; via amide nitrogenUniRule annotation1
Metal bindingi107Magnesium; catalyticUniRule annotation1
Sitei108Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation1
Active sitei131Proton acceptorUniRule annotation1
Binding sitei166Substrate; shared with dimeric partnerUniRule annotation1
Binding sitei226SubstrateUniRule annotation1
Binding sitei270Substrate; shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi76 – 77ATPUniRule annotation2
Nucleotide bindingi106 – 109ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotation
Synonyms:pfk
Ordered Locus Names:CE1348
OrganismiCorynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
Taxonomic identifieri196164 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000001409 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001119471 – 346ATP-dependent 6-phosphofructokinaseAdd BLAST346

Proteomic databases

PRIDEiQ8RQN5

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi196164.HMPREF0290_0720

Structurei

3D structure databases

ProteinModelPortaliQ8RQN5
SMRiQ8RQN5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni129 – 131Substrate bindingUniRule annotation3
Regioni173 – 175Substrate bindingUniRule annotation3
Regioni276 – 279Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CTQ Bacteria
COG0205 LUCA
HOGENOMiHOG000248869
KOiK21071
OMAiGKLHSII
OrthoDBiPOG091H01AC

Family and domain databases

HAMAPiMF_01976 Phosphofructokinase_III, 1 hit
InterProiView protein in InterPro
IPR022953 ATP_PFK
IPR012003 ATP_PFK_prok-type
IPR015912 Phosphofructokinase_CS
IPR000023 Phosphofructokinase_dom
IPR012829 Phosphofructokinase_III
IPR035966 PKF_sf
PfamiView protein in Pfam
PF00365 PFK, 1 hit
PIRSFiPIRSF000532 ATP_PFK_prok, 1 hit
PRINTSiPR00476 PHFRCTKINASE
SUPFAMiSSF53784 SSF53784, 1 hit
TIGRFAMsiTIGR02483 PFK_mixed, 1 hit
PROSITEiView protein in PROSITE
PS00433 PHOSPHOFRUCTOKINASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q8RQN5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGAMRIATLT SGGDCPGLNA VIRGIVRTAS NEFGSTVVGY QDGWEGLLAD
60 70 80 90 100
RRVQLYDDED IDRILLRGGT ILGTGRLHPD KFRAGIDQVK ANLADAGIDA
110 120 130 140 150
LIPIGGEGTL KGAKWLADNG IPVVGVPKTI DNDVNGTDFT FGFDSAVSVA
160 170 180 190 200
TDAIDRLHTT AESHNRVMIV EVMGRHVGWI ALHAGMAGGA HYTVIPEVPF
210 220 230 240 250
DISEICKRME RRFQMGEKYG IIVVAEGALP KEGTMELREG EVDQFGHKTF
260 270 280 290 300
TGIGQQIADE VHRRLGHDVR TTVLGHIQRG GTPTAFDRVL ATRYGVRAAR
310 320 330 340
ACHEGQFNTV VALKGERIRM ISFDEAVGTL KKVPMERWVT AQAMFG
Length:346
Mass (Da):37,476
Last modified:June 1, 2002 - v1
Checksum:i0F233545F77922A7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB083051 Genomic DNA Translation: BAB88667.1
BA000035 Genomic DNA Translation: BAC18158.1

Genome annotation databases

EnsemblBacteriaiBAC18158; BAC18158; BAC18158
KEGGicef:CE1348

Similar proteinsi

Entry informationi

Entry nameiPFKA_COREF
AccessioniPrimary (citable) accession number: Q8RQN5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 2002
Last sequence update: June 1, 2002
Last modified: May 23, 2018
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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