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Q8RQN5 (PFKA_COREF) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATP-dependent 6-phosphofructokinase

Short name=ATP-PFK
Short name=Phosphofructokinase
EC=2.7.1.11
Alternative name(s):
Phosphohexokinase
Gene names
Name:pfkA
Synonyms:pfk
Ordered Locus Names:CE1348
OrganismCorynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395) [Complete proteome] [HAMAP]
Taxonomic identifier196164 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium

Protein attributes

Sequence length346 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity. HAMAP-Rule MF_01976

Catalytic activity

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP-Rule MF_01976

Cofactor

Magnesium By similarity. HAMAP-Rule MF_01976

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP-Rule MF_01976

Subunit structure

Homodimer or homotetramer By similarity. HAMAP-Rule MF_01976

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01976.

Sequence similarities

Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processfructose 6-phosphate metabolic process

Inferred from electronic annotation. Source: InterPro

glycolytic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_component6-phosphofructokinase complex

Inferred from electronic annotation. Source: InterPro

   Molecular_function6-phosphofructokinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

ATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 346346ATP-dependent 6-phosphofructokinase HAMAP-Rule MF_01976
PRO_0000111947

Regions

Nucleotide binding76 – 772ATP By similarity
Nucleotide binding106 – 1094ATP By similarity
Region129 – 1313Substrate binding By similarity
Region173 – 1753Substrate binding By similarity
Region276 – 2794Substrate binding By similarity

Sites

Active site1311Proton acceptor By similarity
Metal binding1071Magnesium; catalytic By similarity
Binding site131ATP; via amide nitrogen By similarity
Binding site1661Substrate; shared with dimeric partner By similarity
Binding site2261Substrate By similarity
Binding site2701Substrate; shared with dimeric partner By similarity
Site1081Important for substrate specificity; cannot use PPi as phosphoryl donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8RQN5 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 0F233545F77922A7

FASTA34637,476
        10         20         30         40         50         60 
MGAMRIATLT SGGDCPGLNA VIRGIVRTAS NEFGSTVVGY QDGWEGLLAD RRVQLYDDED 

        70         80         90        100        110        120 
IDRILLRGGT ILGTGRLHPD KFRAGIDQVK ANLADAGIDA LIPIGGEGTL KGAKWLADNG 

       130        140        150        160        170        180 
IPVVGVPKTI DNDVNGTDFT FGFDSAVSVA TDAIDRLHTT AESHNRVMIV EVMGRHVGWI 

       190        200        210        220        230        240 
ALHAGMAGGA HYTVIPEVPF DISEICKRME RRFQMGEKYG IIVVAEGALP KEGTMELREG 

       250        260        270        280        290        300 
EVDQFGHKTF TGIGQQIADE VHRRLGHDVR TTVLGHIQRG GTPTAFDRVL ATRYGVRAAR 

       310        320        330        340 
ACHEGQFNTV VALKGERIRM ISFDEAVGTL KKVPMERWVT AQAMFG 

« Hide

References

« Hide 'large scale' references
[1]"pfk of Corynebacterium efficiens."
Hirano S., Kimura E., Kawahara Y., Sugimoto S.
Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.
[2]"Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens."
Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T.
Genome Res. 13:1572-1579(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB083051 Genomic DNA. Translation: BAB88667.1.
BA000035 Genomic DNA. Translation: BAC18158.1.
RefSeqNP_737958.1. NC_004369.1.

3D structure databases

ProteinModelPortalQ8RQN5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING196164.CE1348.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC18158; BAC18158; BAC18158.
GeneID1033713.
KEGGcef:CE1348.
PATRIC21488828. VBICorEff9312_1347.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000248869.
KOK00850.
OMAISHRIAT.
OrthoDBEOG644ZRM.

Enzyme and pathway databases

UniPathwayUPA00109; UER00182.

Family and domain databases

HAMAPMF_01976. Phosphofructokinase_III.
InterProIPR012003. ATP_PFK_prok.
IPR012829. PFK.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamPF00365. PFK. 1 hit.
[Graphical view]
PIRSFPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSPR00476. PHFRCTKINASE.
SUPFAMSSF53784. SSF53784. 1 hit.
TIGRFAMsTIGR02483. PFK_mixed. 1 hit.
PROSITEPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePFKA_COREF
AccessionPrimary (citable) accession number: Q8RQN5
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 2002
Last sequence update: June 1, 2002
Last modified: July 9, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways