Reviewed, UniProtKB/Swiss-Prot Q8RQN1 (AK_COREF)

Names and origin

Protein names

Recommended name:
    Aspartokinase
    EC=2.7.2.4
Alternative name(s):
    Aspartate kinase

Gene names

Name:	lysC
Synonyms:	ask
Ordered Locus Names:	CE0220

Organism

Corynebacterium efficiens [Complete proteome] [HAMAP]

Taxonomic identifier

152794 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate = ADP + 4-phospho-L-aspartate.
Enzyme regulation	Feedback inhibition by lysine and threonine.
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 1/4. Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 1/3. Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5.
Subunit structure	Tetramer consisting of 2 isoforms Alpha (catalytic) and 2 isoforms Beta (function not known).
Sequence similarities	Belongs to the aspartokinase family. Contains 2 ACT domains.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Coding sequence diversity	Alternative initiation
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW amino acid binding Inferred from electronic annotation. Source: InterPro aspartate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]

Isoform Alpha (identifier: Q8RQN1-1)

Also known as: Aspartokinase subunit alpha;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Beta (identifier: Q8RQN1-2)

Also known as: Aspartokinase subunit beta;

The sequence of this isoform differs from the canonical sequence as follows:
     1-249: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 421	421	Aspartokinase		PRO_0000002375
Regions
	Domain	266 – 341	76	ACT 1
	Domain	348 – 413	66	ACT 2
Natural variations
	Alternative sequence	1 – 249	249	Missing in isoform Beta.		VSP_018656

Sequences

	Sequence		Length	Mass (Da)
	Isoform Alpha (Aspartokinase subunit alpha) [UniParc]. Last modified June 1, 2002. Version 1. Checksum: F0D638306983CB5F	FASTA	421	44,794
	10 20 30 40 50 60 MALVVQKYGG SSLESAERIR NVAERIVATK KAGNDVVVVC SAMGDTTDEL LDLAAAVNPV 70 80 90 100 110 120 PPAREMDMLL TAGERISNAL VAMAIESLGA EAQSFTGSQA GVLTTERHGN ARIVDVTPGR 130 140 150 160 170 180 VREALDEGKI CIVAGFQGVN KETRDVTTLG RGGSDTTAVA LAAALGADVC EIYSDVDGVY 190 200 210 220 230 240 TADPRIVPNA QKLERLSFEE MLELAAVGSK ILVLRSVEYA RAFNVPMRVR SSYSNDPGTL 250 260 270 280 290 300 IAGSMEDIPM EEAVLTGVAT DKSEAKVTVL GIPDKPGEAA KVFRALADAE INIDMVLQNV 310 320 330 340 350 360 SSVEDGTTDI TFTCPRSDGP RAMELLKKMQ QQGDWTNVLY DDQVGKVSLV GAGMKSHPGV 370 380 390 400 410 420 TAEFMEALRD VNVNVELIST SEIRISVLIR EDDLDKSAKA LHEKFQLGGD EEATVYAGTG R
	Isoform Beta (Aspartokinase subunit beta). Checksum: 9AB776664DCB172E	FASTA	172	18,569
	10 20 30 40 50 60 MEEAVLTGVA TDKSEAKVTV LGIPDKPGEA AKVFRALADA EINIDMVLQN VSSVEDGTTD 70 80 90 100 110 120 ITFTCPRSDG PRAMELLKKM QQQGDWTNVL YDDQVGKVSL VGAGMKSHPG VTAEFMEALR 130 140 150 160 170 DVNVNVELIS TSEIRISVLI REDDLDKSAK ALHEKFQLGG DEEATVYAGT GR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"lysC of Corynebacterium efficiens." Itaya H., Kimura E., Kawahara Y., Sugimoto S. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.
[2]	"Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens." Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T. Genome Res. 13:1572-1579(2003) [PubMed: 12840036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB083129 Genomic DNA. Translation: BAB88820.1. BA000035 Genomic DNA. Translation: BAC17030.1. Different initiation.
RefSeq	NP_736830.1.
3D structure databases
SMR	Q8RQN1. Positions 4-406, 253-416.
ModBase	Search...
Genome annotation databases
GeneID	1034892.
GenomeReviews	Gene locus CE0220 in contig BA000035_GR.
KEGG	cef:CE0220.
NMPDR	fig|196164.1.peg.220.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG724395.
PhylomeDB	Q8RQN1.
Enzyme and pathway databases
BioCyc	CEFF196164:CE0220-MONOMER.
BRENDA	2.7.2.4. 277326.
Family and domain databases
InterPro	IPR002912. ACT_bd. IPR001048. Asp/Glu/Uridylate_kinase. IPR005260. Asp_kin_monofn. IPR001341. Asp_kinase_dom. IPR018042. Aspartate_kinase_CS. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. PF01842. ACT. 2 hits. [Graphical view]
TIGRFAMs	TIGR00656. asp_kin_monofn. 1 hit. TIGR00657. asp_kinases. 1 hit.
PROSITE	PS00324. ASPARTOKINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AK_COREF

Accession

Primary (citable) accession number: Q8RQN1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 2002
Last sequence update:	June 1, 2002
Last modified:	February 9, 2010
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)