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Reviewed, UniProtKB/Swiss-Prot Q8RQL9 (IDH_COREF)

Last modified February 9, 2010. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Isocitrate dehydrogenase [NADP]
      Short name=IDH
    EC=1.1.1.42
Alternative name(s):
    Oxalosuccinate decarboxylase
Gene names
Name: icd
Ordered Locus Names: CE0682
OrganismCorynebacterium efficiens [Complete proteome] [HAMAP]
Taxonomic identifier152794 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

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Protein attributes

Sequence length729 AA.
Sequence statusComplete.
Protein existenceInferred from homology.

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General annotation (Comments)

Catalytic activity

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

Oxalosuccinate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Enzyme regulation

Weakly inhibited by oxaloacetate, 2-oxoglutarate, and citrate. Severely inhibited by oxaloacetate plus glyoxylate By similarity.

Subunit structure

Monomer By similarity.

Sequence similarities

Belongs to the monomeric-type IDH family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 729729Isocitrate dehydrogenase [NADP]
PRO_0000083594

Regions

Nucleotide binding80 – 856NADP By similarity
Nucleotide binding571 – 5722NADP By similarity
Nucleotide binding587 – 5893NADP By similarity
Region121 – 1288Substrate binding By similarity

Sites

Metal binding3371Magnesium or manganese By similarity
Metal binding5351Magnesium or manganese By similarity
Binding site1241NADP By similarity
Binding site1341Substrate By similarity
Binding site5341Substrate By similarity
Binding site5761NADP By similarity
Binding site6361NADP By similarity
Site2441Critical for catalysis By similarity
Site4071Critical for catalysis By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q8RQL9-1 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 84BCDECA8687627B

FASTA72979,285
        10         20         30         40         50         60 
MAKIIWTRTD EAPLLATYSL KPVVEAFAAT AGIEVETRDI SLAGRILAQF ADQLPEEQKV 

        70         80         90        100        110        120 
SDALAELGEL AKTPEANIIK LPNISASVPQ LKAAVKELQE QGYDLPEYED AKDRYAAVIG 

       130        140        150        160        170        180 
SNVNPVLREG NSDRRAPVAV KNFVKKFPHR MGEWSADSKT NVATMGADDF RSNEKSVIMD 

       190        200        210        220        230        240 
EADTVVIKHV AADGTETVLK DSLPLLKGEV IDGTFISAKA LDAFLLDQVK RAKEEGILFS 

       250        260        270        280        290        300 
AHMKATMMKV SDPIIFGHIV RAYFADVYAQ YGEQLLAAGL NGENGLAAIY AGLDKLDNGA 

       310        320        330        340        350        360 
EIKAAFDKGL EEGPDLAMVN SAKGITNLHV PSDVIIDASM PAMIRTSGKM WNKDDQTQDA 

       370        380        390        400        410        420 
LAVIPDSSYA GVYQTVIEDC RKNGAFDPTT MGTVPNVGLM AQKAEEYGSH DKTFRIEADG 

       430        440        450        460        470        480 
KVQVVASNGD VLIEHDVEKG DIWRACQTKD APIQDWVKLA VNRARLSGMP AVFWLDPARA 

       490        500        510        520        530        540 
HDRNLTTLVE KYLADHDTEG LDIQILSPVE ATQHAIDRIR RGEDTISVTG NVLRDYNTDL 

       550        560        570        580        590        600 
FPILELGTSA KMLSVVPLMA GGGLFETGAG GSAPKHVQQV IEENHLRWDS LGEFLALAES 

       610        620        630        640        650        660 
FRHELNTRNN TKAGVLADAL DRATEKLLNE EKSPSRKVGE IDNRGSHFWL ATYWADELAN 

       670        680        690        700        710        720 
QTEDAELAET FAPVAEALNN QAADIDAALI GEQGKPVDLG GYYAPSDEKT SAIMRPVAAF 


NEIIDSLKK

« Hide

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References

« Hide 'large scale' references
[1]"Corynebacterium efficiens icd gene for isocitrate dehydrogenase, complete cds."
Nonaka G., Kimura E., Kawahara Y., Sugimoto S.
Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.
[2]"Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens."
Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T.
Genome Res. 13:1572-1579(2003) [PubMed: 12840036] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AB083179 Genomic DNA. Translation: BAB88832.1.
BA000035 Genomic DNA. Translation: BAC17492.1. Different initiation.
RefSeqNP_737292.1.

3D structure databases

SMRQ8RQL9. Positions 2-727.
ModBaseSearch...

Genome annotation databases

GeneID1033167.
GenomeReviewsGene locus CE0682 in contig BA000035_GR.
KEGGcef:CE0682.
NMPDRfig|196164.1.peg.682.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG286619.

Enzyme and pathway databases

BioCycCEFF196164:CE0682-MONOMER.
BRENDA1.1.1.42. 277326.

Family and domain databases

InterProIPR004436. Isocitrate_DH_NADP_mono.
[Graphical view]
PfamPF03971. IDH. 1 hit.
[Graphical view]
PIRSFPIRSF009407. IDH_monmr. 1 hit.
TIGRFAMsTIGR00178. monomer_idh. 1 hit.
ProtoNetSearch...

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Entry information

Entry nameIDH_COREF
AccessionPrimary (citable) accession number: Q8RQL9
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 2002
Last sequence update: June 1, 2002
Last modified: February 9, 2010
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents