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Protein

Isocitrate dehydrogenase [NADP]

Gene

icd

Organism
Corynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 1 Mg2+ or Mn2+ ion per subunit.By similarity

Enzyme regulationi

Weakly inhibited by oxaloacetate, 2-oxoglutarate, and citrate. Severely inhibited by oxaloacetate plus glyoxylate (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei124NADPBy similarity1
Binding sitei134SubstrateBy similarity1
Sitei244Critical for catalysisBy similarity1
Metal bindingi337Magnesium or manganeseBy similarity1
Sitei407Critical for catalysisBy similarity1
Binding sitei534SubstrateBy similarity1
Metal bindingi535Magnesium or manganeseBy similarity1
Metal bindingi539Magnesium or manganeseBy similarity1
Binding sitei576NADPBy similarity1
Binding sitei636NADPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi80 – 85NADPBy similarity6
Nucleotide bindingi571 – 572NADPBy similarity2
Nucleotide bindingi587 – 589NADPBy similarity3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NADP] (EC:1.1.1.42)
Short name:
IDH
Alternative name(s):
Oxalosuccinate decarboxylase
Gene namesi
Name:icd
Ordered Locus Names:CE0682
OrganismiCorynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
Taxonomic identifieri196164 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000001409 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000835941 – 729Isocitrate dehydrogenase [NADP]Add BLAST729

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi196164.HMPREF0290_1047.

Structurei

3D structure databases

ProteinModelPortaliQ8RQL9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni121 – 128Substrate bindingBy similarity8

Sequence similaritiesi

Belongs to the monomeric-type IDH family.Curated

Phylogenomic databases

eggNOGiCOG2838. LUCA.
HOGENOMiHOG000240860.
KOiK00031.
OrthoDBiPOG091H0B3N.

Family and domain databases

Gene3Di3.40.718.10. 3 hits.
InterProiIPR004436. Isocitrate_DH_NADP_mono.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PfamiPF03971. IDH. 1 hit.
[Graphical view]
PIRSFiPIRSF009407. IDH_monmr. 1 hit.
TIGRFAMsiTIGR00178. monomer_idh. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8RQL9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKIIWTRTD EAPLLATYSL KPVVEAFAAT AGIEVETRDI SLAGRILAQF
60 70 80 90 100
ADQLPEEQKV SDALAELGEL AKTPEANIIK LPNISASVPQ LKAAVKELQE
110 120 130 140 150
QGYDLPEYED AKDRYAAVIG SNVNPVLREG NSDRRAPVAV KNFVKKFPHR
160 170 180 190 200
MGEWSADSKT NVATMGADDF RSNEKSVIMD EADTVVIKHV AADGTETVLK
210 220 230 240 250
DSLPLLKGEV IDGTFISAKA LDAFLLDQVK RAKEEGILFS AHMKATMMKV
260 270 280 290 300
SDPIIFGHIV RAYFADVYAQ YGEQLLAAGL NGENGLAAIY AGLDKLDNGA
310 320 330 340 350
EIKAAFDKGL EEGPDLAMVN SAKGITNLHV PSDVIIDASM PAMIRTSGKM
360 370 380 390 400
WNKDDQTQDA LAVIPDSSYA GVYQTVIEDC RKNGAFDPTT MGTVPNVGLM
410 420 430 440 450
AQKAEEYGSH DKTFRIEADG KVQVVASNGD VLIEHDVEKG DIWRACQTKD
460 470 480 490 500
APIQDWVKLA VNRARLSGMP AVFWLDPARA HDRNLTTLVE KYLADHDTEG
510 520 530 540 550
LDIQILSPVE ATQHAIDRIR RGEDTISVTG NVLRDYNTDL FPILELGTSA
560 570 580 590 600
KMLSVVPLMA GGGLFETGAG GSAPKHVQQV IEENHLRWDS LGEFLALAES
610 620 630 640 650
FRHELNTRNN TKAGVLADAL DRATEKLLNE EKSPSRKVGE IDNRGSHFWL
660 670 680 690 700
ATYWADELAN QTEDAELAET FAPVAEALNN QAADIDAALI GEQGKPVDLG
710 720
GYYAPSDEKT SAIMRPVAAF NEIIDSLKK
Length:729
Mass (Da):79,285
Last modified:June 1, 2002 - v1
Checksum:i84BCDECA8687627B
GO

Sequence cautioni

The sequence BAC17492 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB083179 Genomic DNA. Translation: BAB88832.1.
BA000035 Genomic DNA. Translation: BAC17492.1. Different initiation.
RefSeqiWP_006769639.1. NZ_GG700687.1.

Genome annotation databases

EnsemblBacteriaiBAC17492; BAC17492; BAC17492.
KEGGicef:CE0682.
PATRICi21487524. VBICorEff9312_0704.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB083179 Genomic DNA. Translation: BAB88832.1.
BA000035 Genomic DNA. Translation: BAC17492.1. Different initiation.
RefSeqiWP_006769639.1. NZ_GG700687.1.

3D structure databases

ProteinModelPortaliQ8RQL9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi196164.HMPREF0290_1047.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC17492; BAC17492; BAC17492.
KEGGicef:CE0682.
PATRICi21487524. VBICorEff9312_0704.

Phylogenomic databases

eggNOGiCOG2838. LUCA.
HOGENOMiHOG000240860.
KOiK00031.
OrthoDBiPOG091H0B3N.

Family and domain databases

Gene3Di3.40.718.10. 3 hits.
InterProiIPR004436. Isocitrate_DH_NADP_mono.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PfamiPF03971. IDH. 1 hit.
[Graphical view]
PIRSFiPIRSF009407. IDH_monmr. 1 hit.
TIGRFAMsiTIGR00178. monomer_idh. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiIDH_COREF
AccessioniPrimary (citable) accession number: Q8RQL9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 2002
Last sequence update: June 1, 2002
Last modified: November 2, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.