Q8RQL3 (CAPP_COREF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoenolpyruvate carboxylase
Short name=PEPC
Short name=PEPCase
EC=4.1.1.31

Gene names

Name:	ppc
Ordered Locus Names:	CE1703

Organism

Corynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395) [Complete proteome] [HAMAP]

Taxonomic identifier

196164 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium › Corynebacterium efficiens

Protein attributes

Sequence length	919 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Forms oxaloacetate, a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle By similarity. HAMAP-Rule MF_00595
Catalytic activity	Phosphate + oxaloacetate = H2O + phosphoenolpyruvate + HCO3-. HAMAP-Rule MF_00595
Cofactor	Magnesium By similarity.
Sequence similarities	Belongs to the PEPCase type 1 family.

Ontologies

Keywords
Biological process	Carbon dioxide fixation
Ligand	Magnesium
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	carbon fixation Inferred from electronic annotation. Source: HAMAP oxaloacetate metabolic process Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: InterPro
Molecular_function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP phosphoenolpyruvate carboxylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 919	919	Phosphoenolpyruvate carboxylase HAMAP-Rule MF_00595		PRO_0000166590
Sites
	Active site	138	1	By similarity
	Active site	579	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8RQL3 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: E2D8FE8DD5BC6E51	FASTA	919	103,096
	10 20 30 40 50 60 MNELLRDDIR YLGRILGEVI SEQEGHHVFE LVERARRTSF DIAKGRAEMD SLVEVFAGID 70 80 90 100 110 120 PEDATPVARA FTHFALLANL AEDLHDAAQR EQALNSGEPA PDSTLEATWV KLDDAGVGSG 130 140 150 160 170 180 EVAAVIRNAL VAPVLTAHPT ETRRRTVFDA QKHITALMEE RHLLLALPTH ARTQSKLDDI 190 200 210 220 230 240 ERNIRRRITI LWQTALIRVA RPRIEDEVEV GLRYYKLSLL AEIPRINHDV TVELARRFGG 250 260 270 280 290 300 DIPTTAMVRP GSWIGGDHDG NPFVTAETVT YATHRAAETV LKYYVKQLHA LEHELSLSDR 310 320 330 340 350 360 MNVISDELRV LADAGQNDMP SRVDEPYRRA IHGMRGRMLA TTAALIGEEA VEGTWFKTFT 370 380 390 400 410 420 PYTDTHEFKR DLDIVDGSLR MSRDDIIADD RLAMLRSALD SFGFNLYSLD LRQNSDGFED 430 440 450 460 470 480 VLTELFATAQ TEKNYRGLTE AEKLDLLIRE LSTPRPLIPH GDPDYSEATN RELGIFSKAA 490 500 510 520 530 540 EAVRKFGPLM VPHCIISMAS SVTDILEPMV LLKEFGLIRA NGKNPTGSVD VIPLFETIDD 550 560 570 580 590 600 LQRGAGILEE LWDIDLYRNY LEQRDNVQEV MLGYSDSNKD GGYFAANWAL YDAELRLVEL 610 620 630 640 650 660 CRGRNVKLRL FHGRGGTVGR GGGPSYDAIL AQPKGAVRGA VRVTEQGEII SAKYGNPDTA 670 680 690 700 710 720 RRNLEALVSA TLEASLLDDV ELPNRERAHQ IMGEISELSF RRYSSLVHED PGFIQYFTQS 730 740 750 760 770 780 TPLQEIGSLN IGSRPSSRKQ TNTVEDLRAI PWVLSWSQSR VMLPGWFGVG TALREWIGEG 790 800 810 820 830 840 EGAAERIAEL QELNRCWPFF TSVLDNMAQV MSKAELRLAR LYADLIPDRE VADRIYETIF 850 860 870 880 890 900 GEYFLTKEMF CTITGSQDLL DDNPALARSV RSRFPYLLPL NVIQVEMMRR YRSGDEGTAV 910 PRNIRLTMNG LSTALRNSG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Corynebacterium efficiens ppc gene for phosphoenolpyruvate carboxylase, complete CDS." Akiyoshi N., Nonaka G., Kimura E., Kawahara Y., Sugimoto S. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.
[2]	"Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens." Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T. Genome Res. 13:1572-1579(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB083298 Genomic DNA. Translation: BAB88902.1. BA000035 Genomic DNA. Translation: BAC18513.1.
RefSeq	NP_738313.1. NC_004369.1.
3D structure databases
ProteinModelPortal	Q8RQL3.
ModBase	Search...
Protein-protein interaction databases
STRING	196164.CE1703.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAC18513; BAC18513; BAC18513.
GeneID	1034200.
KEGG	cef:CE1703.
PATRIC	21489547. VBICorEff9312_1694.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000238647.
KO	K01595.
OMA	AIPWVFG.
ProtClustDB	PRK00009.
Family and domain databases
HAMAP	MF_00595. PEPcase_type1.
InterPro	IPR021135. PEP_COase. IPR018129. PEP_COase_AS. IPR022805. PEP_COase_bac/pln-type. IPR015813. Pyrv/PenolPyrv_Kinase. [Graphical view]
Pfam	PF00311. PEPcase. 1 hit. [Graphical view]
PRINTS	PR00150. PEPCARBXLASE.
SUPFAM	SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
PROSITE	PS00781. PEPCASE_1. 1 hit. PS00393. PEPCASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CAPP_COREF

Accession

Primary (citable) accession number: Q8RQL3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 2002
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program