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Protein

DNA-directed RNA polymerase subunit beta

Gene

rpoB

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Transcription

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase subunit betaUniRule annotation (EC:2.7.7.6UniRule annotation)
Short name:
RNAP subunit betaUniRule annotation
Alternative name(s):
RNA polymerase subunit betaUniRule annotation
Transcriptase subunit betaUniRule annotation
Gene namesi
Name:rpoBUniRule annotation
Ordered Locus Names:TTHA1813
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Subcellular locationi

Keywords - Cellular componenti

DNA-directed RNA polymerase

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000479851 – 1119DNA-directed RNA polymerase subunit betaAdd BLAST1119

Interactioni

Subunit structurei

The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.

Protein-protein interaction databases

DIPiDIP-47011N.
IntActiQ8RQE9. 3 interactors.
STRINGi300852.TTHA1813.

Structurei

Secondary structure

11119
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 5Combined sources4
Helixi20 – 29Combined sources10
Helixi31 – 33Combined sources3
Helixi36 – 38Combined sources3
Helixi43 – 51Combined sources9
Beta strandi53 – 56Combined sources4
Beta strandi59 – 61Combined sources3
Beta strandi64 – 68Combined sources5
Helixi80 – 85Combined sources6
Beta strandi91 – 94Combined sources4
Beta strandi98 – 100Combined sources3
Beta strandi103 – 105Combined sources3
Beta strandi107 – 109Combined sources3
Beta strandi117 – 119Combined sources3
Beta strandi123 – 125Combined sources3
Beta strandi129 – 132Combined sources4
Beta strandi134 – 136Combined sources3
Beta strandi138 – 142Combined sources5
Beta strandi146 – 149Combined sources4
Beta strandi152 – 156Combined sources5
Beta strandi159 – 162Combined sources4
Beta strandi166 – 169Combined sources4
Beta strandi172 – 177Combined sources6
Turni178 – 180Combined sources3
Beta strandi181 – 191Combined sources11
Helixi193 – 200Combined sources8
Helixi204 – 212Combined sources9
Turni215 – 217Combined sources3
Helixi218 – 221Combined sources4
Helixi225 – 228Combined sources4
Helixi231 – 241Combined sources11
Beta strandi242 – 244Combined sources3
Turni250 – 252Combined sources3
Helixi253 – 257Combined sources5
Beta strandi259 – 264Combined sources6
Turni269 – 271Combined sources3
Helixi274 – 278Combined sources5
Beta strandi281 – 283Combined sources3
Turni287 – 290Combined sources4
Beta strandi294 – 296Combined sources3
Beta strandi297 – 302Combined sources6
Helixi303 – 314Combined sources12
Beta strandi325 – 327Combined sources3
Turni328 – 330Combined sources3
Beta strandi331 – 334Combined sources4
Helixi336 – 361Combined sources26
Beta strandi364 – 366Combined sources3
Turni370 – 372Combined sources3
Helixi376 – 387Combined sources12
Beta strandi390 – 394Combined sources5
Helixi401 – 408Combined sources8
Beta strandi409 – 418Combined sources10
Turni420 – 422Combined sources3
Helixi425 – 428Combined sources4
Helixi432 – 434Combined sources3
Turni435 – 437Combined sources3
Beta strandi440 – 443Combined sources4
Beta strandi445 – 448Combined sources4
Beta strandi450 – 455Combined sources6
Beta strandi456 – 458Combined sources3
Beta strandi460 – 462Combined sources3
Beta strandi463 – 465Combined sources3
Beta strandi467 – 475Combined sources9
Beta strandi478 – 486Combined sources9
Helixi488 – 493Combined sources6
Beta strandi494 – 497Combined sources4
Beta strandi499 – 501Combined sources3
Beta strandi503 – 508Combined sources6
Beta strandi511 – 524Combined sources14
Turni526 – 528Combined sources3
Beta strandi531 – 533Combined sources3
Turni536 – 539Combined sources4
Helixi542 – 545Combined sources4
Helixi550 – 552Combined sources3
Helixi555 – 565Combined sources11
Beta strandi571 – 573Combined sources3
Beta strandi578 – 580Combined sources3
Helixi584 – 590Combined sources7
Beta strandi594 – 596Combined sources3
Beta strandi598 – 606Combined sources9
Beta strandi608 – 615Combined sources8
Turni616 – 618Combined sources3
Beta strandi619 – 621Combined sources3
Beta strandi628 – 630Combined sources3
Beta strandi634 – 637Combined sources4
Beta strandi654 – 657Combined sources4
Beta strandi661 – 666Combined sources6
Beta strandi669 – 675Combined sources7
Turni680 – 683Combined sources4
Beta strandi684 – 691Combined sources8
Helixi693 – 696Combined sources4
Turni697 – 700Combined sources4
Beta strandi703 – 713Combined sources11
Beta strandi727 – 729Combined sources3
Helixi731 – 734Combined sources4
Helixi735 – 737Combined sources3
Turni738 – 740Combined sources3
Beta strandi741 – 743Combined sources3
Beta strandi754 – 756Combined sources3
Beta strandi758 – 761Combined sources4
Beta strandi762 – 766Combined sources5
Helixi769 – 778Combined sources10
Beta strandi785 – 787Combined sources3
Beta strandi798 – 807Combined sources10
Beta strandi808 – 810Combined sources3
Beta strandi819 – 829Combined sources11
Beta strandi838 – 840Combined sources3
Beta strandi842 – 844Combined sources3
Beta strandi846 – 853Combined sources8
Turni855 – 857Combined sources3
Beta strandi862 – 864Combined sources3
Beta strandi868 – 870Combined sources3
Turni874 – 880Combined sources7
Helixi883 – 896Combined sources14
Beta strandi899 – 902Combined sources4
Turni905 – 907Combined sources3
Helixi911 – 932Combined sources22
Helixi938 – 948Combined sources11
Turni949 – 951Combined sources3
Beta strandi955 – 957Combined sources3
Helixi959 – 968Combined sources10
Beta strandi977 – 979Combined sources3
Beta strandi980 – 982Combined sources3
Beta strandi987 – 997Combined sources11
Turni1001 – 1003Combined sources3
Beta strandi1006 – 1010Combined sources5
Beta strandi1015 – 1017Combined sources3
Beta strandi1024 – 1026Combined sources3
Beta strandi1030 – 1032Combined sources3
Helixi1034 – 1042Combined sources9
Helixi1047 – 1053Combined sources7
Turni1054 – 1058Combined sources5
Helixi1060 – 1071Combined sources12
Helixi1083 – 1094Combined sources12
Beta strandi1098 – 1102Combined sources5
Beta strandi1104 – 1106Combined sources3
Beta strandi1114 – 1117Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IW7X-ray2.60C/M1-1119[»]
1SMYX-ray2.70C/M1-1119[»]
1ZYRX-ray3.00C/M1-1119[»]
2A68X-ray2.50C/M1-1119[»]
2A69X-ray2.50C/M1-1119[»]
2A6EX-ray2.80C/M1-1119[»]
2A6HX-ray2.40C/M1-1119[»]
2BE5X-ray2.40C/M1-1119[»]
2CW0X-ray3.30C/M1-1119[»]
2O5IX-ray2.50C/M1-1119[»]
2O5JX-ray3.00C/M1-1119[»]
2PPBX-ray3.00C/M1-1119[»]
3AOHX-ray4.10C/H/M1-1119[»]
3AOIX-ray4.30C/H/M1-1119[»]
3DXJX-ray3.00C/M1-1119[»]
3EQLX-ray2.70C/M1-1119[»]
3WODX-ray3.60C1-1119[»]
3WOEX-ray2.35A/C703-830[»]
3WOFX-ray3.30A/C/E/G/I/K/M/O/Q/S/U/W703-830[»]
4G7HX-ray2.90C/M1-1119[»]
4G7OX-ray2.99C/M1-1119[»]
4G7ZX-ray3.82C/M1-1119[»]
4GZYX-ray3.51C1-1119[»]
4GZZX-ray4.29C1-1119[»]
4MQ9X-ray3.35C1-1119[»]
4OINX-ray2.80C1-1119[»]
4OIOX-ray3.10C1-1119[»]
4OIPX-ray3.40C1-1119[»]
4OIQX-ray3.62C1-1119[»]
4OIRX-ray3.10C1-1119[»]
4Q4ZX-ray2.90C1-1119[»]
4Q5SX-ray3.00C1-1119[»]
4WQSX-ray4.31C/M1-1119[»]
4WQTX-ray4.40C/H/M1-1119[»]
5D4CX-ray3.28C/M1-1119[»]
5D4DX-ray3.00C/M1-1119[»]
5D4EX-ray3.08C/M1-1119[»]
5E17X-ray3.20C1-1119[»]
5E18X-ray3.30C1-1119[»]
5I2DX-ray4.41C/N1-1119[»]
ProteinModelPortaliQ8RQE9.
SMRiQ8RQE9.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8RQE9.

Family & Domainsi

Sequence similaritiesi

Belongs to the RNA polymerase beta chain family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108IIJ. Bacteria.
COG0085. LUCA.
HOGENOMiHOG000218611.
KOiK03043.
OMAiWWMFAVE.
PhylomeDBiQ8RQE9.

Family and domain databases

CDDicd00653. RNA_pol_B_RPB2. 1 hit.
Gene3Di2.30.150.10. 1 hit.
2.40.270.10. 2 hits.
2.40.50.150. 1 hit.
3.90.1110.10. 1 hit.
HAMAPiMF_01321. RNApol_bact_RpoB. 1 hit.
InterProiIPR019462. DNA-dir_RNA_pol_bsu_external_1.
IPR015712. DNA-dir_RNA_pol_su2.
IPR007120. DNA-dir_RNA_pol_su2_6.
IPR010243. RNA_pol_bsu_bac.
IPR007121. RNA_pol_bsu_CS.
IPR007644. RNA_pol_bsu_protrusion.
IPR007642. RNA_pol_Rpb2_2.
IPR007645. RNA_pol_Rpb2_3.
IPR007641. RNA_pol_Rpb2_7.
IPR014724. RNA_pol_RPB2_OB-fold.
[Graphical view]
PANTHERiPTHR20856. PTHR20856. 2 hits.
PfamiPF04563. RNA_pol_Rpb2_1. 1 hit.
PF04561. RNA_pol_Rpb2_2. 1 hit.
PF04565. RNA_pol_Rpb2_3. 1 hit.
PF10385. RNA_pol_Rpb2_45. 1 hit.
PF00562. RNA_pol_Rpb2_6. 1 hit.
PF04560. RNA_pol_Rpb2_7. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02013. rpoB. 1 hit.
PROSITEiPS01166. RNA_POL_BETA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8RQE9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEIKRFGRIR EVIPLPPLTE IQVESYRRAL QADVPPEKRE NVGIQAAFRE
60 70 80 90 100
TFPIEEEDKG KGGLVLDFLE YRLGEPPFPQ DECREKDLTY QAPLYARLQL
110 120 130 140 150
IHKDTGLIKE DEVFLGHIPL MTEDGSFIIN GADRVIVSQI HRSPGVYFTP
160 170 180 190 200
DPARPGRYIA SIIPLPKRGP WIDLEVEPNG VVSMKVNKRK FPLVLLLRVL
210 220 230 240 250
GYDQETLARE LGAYGELVQG LMDESVFAMR PEEALIRLFT LLRPGDPPKR
260 270 280 290 300
DKAVAYVYGL IADPRRYDLG EAGRYKAEEK LGIRLSGRTL ARFEDGEFKD
310 320 330 340 350
EVFLPTLRYL FALTAGVPGH EVDDIDHLGN RRIRTVGELM TDQFRVGLAR
360 370 380 390 400
LARGVRERML MGSEDSLTPA KLVNSRPLEA AIREFFSRSQ LSQFKDETNP
410 420 430 440 450
LSSLRHKRRI SALGPGGLTR ERAGFDVRDV HRTHYGRICP VETPEGANIG
460 470 480 490 500
LITSLAAYAR VDELGFIRTP YRRVVGGVVT DEVVYMTATE EDRYTIAQAN
510 520 530 540 550
TPLEGNRIAA ERVVARRKGE PVIVSPEEVE FMDVSPKQVF SVNTNLIPFL
560 570 580 590 600
EHDDANRALM GSNMQTQAVP LIRAQAPVVM TGLEERVVRD SLAALYAEED
610 620 630 640 650
GEVAKVDGNR IVVRYEDGRL VEYPLRRFYR SNQGTALDQR PRVVVGQRVR
660 670 680 690 700
KGDLLADGPA SENGFLALGQ NVLVAIMPFD GYNFEDAIVI SEELLKRDFY
710 720 730 740 750
TSIHIERYEI EARDTKLGPE RITRDIPHLS EAALRDLDEE GVVRIGAEVK
760 770 780 790 800
PGDILVGRTS FKGESEPTPE ERLLRSIFGE KARDVKDTSL RVPPGEGGIV
810 820 830 840 850
VRTVRLRRGD PGVELKPGVR EVVRVYVAQK RKLQVGDKLA NRHGNKGVVA
860 870 880 890 900
KILPVEDMPH LPDGTPVDVI LNPLGVPSRM NLGQILETHL GLAGYFLGQR
910 920 930 940 950
YISPIFDGAK EPEIKELLAQ AFEVYFGKRK GEGFGVDKRE VEVLRRAEKL
960 970 980 990 1000
GLVTPGKTPE EQLKELFLQG KVVLYDGRTG EPIEGPIVVG QMFIMKLYHM
1010 1020 1030 1040 1050
VEDKMHARST GPYSLITQQP LGGKAQFGGQ RFGEMEVWAL EAYGAAHTLQ
1060 1070 1080 1090 1100
EMLTLKSDDI EGRNAAYEAI IKGEDVPEPS VPESFRVLVK ELQALALDVQ
1110
TLDEKDNPVD IFEGLASKR
Length:1,119
Mass (Da):125,264
Last modified:June 1, 2002 - v1
Checksum:iC1260F31CEC6B40D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB083789 Genomic DNA. Translation: BAB89400.1.
AP008226 Genomic DNA. Translation: BAD71636.1.
RefSeqiWP_011228935.1. NC_006461.1.
YP_145079.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD71636; BAD71636; BAD71636.
GeneIDi3169736.
KEGGittj:TTHA1813.
PATRICi23958595. VBITheThe93045_1784.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB083789 Genomic DNA. Translation: BAB89400.1.
AP008226 Genomic DNA. Translation: BAD71636.1.
RefSeqiWP_011228935.1. NC_006461.1.
YP_145079.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IW7X-ray2.60C/M1-1119[»]
1SMYX-ray2.70C/M1-1119[»]
1ZYRX-ray3.00C/M1-1119[»]
2A68X-ray2.50C/M1-1119[»]
2A69X-ray2.50C/M1-1119[»]
2A6EX-ray2.80C/M1-1119[»]
2A6HX-ray2.40C/M1-1119[»]
2BE5X-ray2.40C/M1-1119[»]
2CW0X-ray3.30C/M1-1119[»]
2O5IX-ray2.50C/M1-1119[»]
2O5JX-ray3.00C/M1-1119[»]
2PPBX-ray3.00C/M1-1119[»]
3AOHX-ray4.10C/H/M1-1119[»]
3AOIX-ray4.30C/H/M1-1119[»]
3DXJX-ray3.00C/M1-1119[»]
3EQLX-ray2.70C/M1-1119[»]
3WODX-ray3.60C1-1119[»]
3WOEX-ray2.35A/C703-830[»]
3WOFX-ray3.30A/C/E/G/I/K/M/O/Q/S/U/W703-830[»]
4G7HX-ray2.90C/M1-1119[»]
4G7OX-ray2.99C/M1-1119[»]
4G7ZX-ray3.82C/M1-1119[»]
4GZYX-ray3.51C1-1119[»]
4GZZX-ray4.29C1-1119[»]
4MQ9X-ray3.35C1-1119[»]
4OINX-ray2.80C1-1119[»]
4OIOX-ray3.10C1-1119[»]
4OIPX-ray3.40C1-1119[»]
4OIQX-ray3.62C1-1119[»]
4OIRX-ray3.10C1-1119[»]
4Q4ZX-ray2.90C1-1119[»]
4Q5SX-ray3.00C1-1119[»]
4WQSX-ray4.31C/M1-1119[»]
4WQTX-ray4.40C/H/M1-1119[»]
5D4CX-ray3.28C/M1-1119[»]
5D4DX-ray3.00C/M1-1119[»]
5D4EX-ray3.08C/M1-1119[»]
5E17X-ray3.20C1-1119[»]
5E18X-ray3.30C1-1119[»]
5I2DX-ray4.41C/N1-1119[»]
ProteinModelPortaliQ8RQE9.
SMRiQ8RQE9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-47011N.
IntActiQ8RQE9. 3 interactors.
STRINGi300852.TTHA1813.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD71636; BAD71636; BAD71636.
GeneIDi3169736.
KEGGittj:TTHA1813.
PATRICi23958595. VBITheThe93045_1784.

Phylogenomic databases

eggNOGiENOG4108IIJ. Bacteria.
COG0085. LUCA.
HOGENOMiHOG000218611.
KOiK03043.
OMAiWWMFAVE.
PhylomeDBiQ8RQE9.

Miscellaneous databases

EvolutionaryTraceiQ8RQE9.
PROiQ8RQE9.

Family and domain databases

CDDicd00653. RNA_pol_B_RPB2. 1 hit.
Gene3Di2.30.150.10. 1 hit.
2.40.270.10. 2 hits.
2.40.50.150. 1 hit.
3.90.1110.10. 1 hit.
HAMAPiMF_01321. RNApol_bact_RpoB. 1 hit.
InterProiIPR019462. DNA-dir_RNA_pol_bsu_external_1.
IPR015712. DNA-dir_RNA_pol_su2.
IPR007120. DNA-dir_RNA_pol_su2_6.
IPR010243. RNA_pol_bsu_bac.
IPR007121. RNA_pol_bsu_CS.
IPR007644. RNA_pol_bsu_protrusion.
IPR007642. RNA_pol_Rpb2_2.
IPR007645. RNA_pol_Rpb2_3.
IPR007641. RNA_pol_Rpb2_7.
IPR014724. RNA_pol_RPB2_OB-fold.
[Graphical view]
PANTHERiPTHR20856. PTHR20856. 2 hits.
PfamiPF04563. RNA_pol_Rpb2_1. 1 hit.
PF04561. RNA_pol_Rpb2_2. 1 hit.
PF04565. RNA_pol_Rpb2_3. 1 hit.
PF10385. RNA_pol_Rpb2_45. 1 hit.
PF00562. RNA_pol_Rpb2_6. 1 hit.
PF04560. RNA_pol_Rpb2_7. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02013. rpoB. 1 hit.
PROSITEiPS01166. RNA_POL_BETA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRPOB_THET8
AccessioniPrimary (citable) accession number: Q8RQE9
Secondary accession number(s): Q5SHB7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: June 1, 2002
Last modified: November 2, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.