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Q8RQE9 (RPOB_THET8) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA-directed RNA polymerase subunit beta
Short name=RNAP subunit beta
EC=2.7.7.6
Alternative name(s):
RNA polymerase subunit beta
Transcriptase subunit beta
Gene names
Name:rpoB
Ordered Locus Names:TTHA1813
OrganismThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Reference proteome] [HAMAP]
Taxonomic identifier300852 [NCBI]
Taxonomic lineageBacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus ›

Protein attributes

Sequence length1119 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. HAMAP-Rule MF_01321

Catalytic activity

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP-Rule MF_01321

Subunit structure

The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.

Sequence similarities

Belongs to the RNA polymerase beta chain family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11191119DNA-directed RNA polymerase subunit beta HAMAP-Rule MF_01321
PRO_0000047985

Secondary structure

1 ...................................................................................................................................................................................................................................................... 1119
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q8RQE9 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: C1260F31CEC6B40D

FASTA1,119125,264
        10         20         30         40         50         60 
MEIKRFGRIR EVIPLPPLTE IQVESYRRAL QADVPPEKRE NVGIQAAFRE TFPIEEEDKG 

        70         80         90        100        110        120 
KGGLVLDFLE YRLGEPPFPQ DECREKDLTY QAPLYARLQL IHKDTGLIKE DEVFLGHIPL 

       130        140        150        160        170        180 
MTEDGSFIIN GADRVIVSQI HRSPGVYFTP DPARPGRYIA SIIPLPKRGP WIDLEVEPNG 

       190        200        210        220        230        240 
VVSMKVNKRK FPLVLLLRVL GYDQETLARE LGAYGELVQG LMDESVFAMR PEEALIRLFT 

       250        260        270        280        290        300 
LLRPGDPPKR DKAVAYVYGL IADPRRYDLG EAGRYKAEEK LGIRLSGRTL ARFEDGEFKD 

       310        320        330        340        350        360 
EVFLPTLRYL FALTAGVPGH EVDDIDHLGN RRIRTVGELM TDQFRVGLAR LARGVRERML 

       370        380        390        400        410        420 
MGSEDSLTPA KLVNSRPLEA AIREFFSRSQ LSQFKDETNP LSSLRHKRRI SALGPGGLTR 

       430        440        450        460        470        480 
ERAGFDVRDV HRTHYGRICP VETPEGANIG LITSLAAYAR VDELGFIRTP YRRVVGGVVT 

       490        500        510        520        530        540 
DEVVYMTATE EDRYTIAQAN TPLEGNRIAA ERVVARRKGE PVIVSPEEVE FMDVSPKQVF 

       550        560        570        580        590        600 
SVNTNLIPFL EHDDANRALM GSNMQTQAVP LIRAQAPVVM TGLEERVVRD SLAALYAEED 

       610        620        630        640        650        660 
GEVAKVDGNR IVVRYEDGRL VEYPLRRFYR SNQGTALDQR PRVVVGQRVR KGDLLADGPA 

       670        680        690        700        710        720 
SENGFLALGQ NVLVAIMPFD GYNFEDAIVI SEELLKRDFY TSIHIERYEI EARDTKLGPE 

       730        740        750        760        770        780 
RITRDIPHLS EAALRDLDEE GVVRIGAEVK PGDILVGRTS FKGESEPTPE ERLLRSIFGE 

       790        800        810        820        830        840 
KARDVKDTSL RVPPGEGGIV VRTVRLRRGD PGVELKPGVR EVVRVYVAQK RKLQVGDKLA 

       850        860        870        880        890        900 
NRHGNKGVVA KILPVEDMPH LPDGTPVDVI LNPLGVPSRM NLGQILETHL GLAGYFLGQR 

       910        920        930        940        950        960 
YISPIFDGAK EPEIKELLAQ AFEVYFGKRK GEGFGVDKRE VEVLRRAEKL GLVTPGKTPE 

       970        980        990       1000       1010       1020 
EQLKELFLQG KVVLYDGRTG EPIEGPIVVG QMFIMKLYHM VEDKMHARST GPYSLITQQP 

      1030       1040       1050       1060       1070       1080 
LGGKAQFGGQ RFGEMEVWAL EAYGAAHTLQ EMLTLKSDDI EGRNAAYEAI IKGEDVPEPS 

      1090       1100       1110 
VPESFRVLVK ELQALALDVQ TLDEKDNPVD IFEGLASKR

« Hide

References

« Hide 'large scale' references
[1]"Purification, crystallization and initial crystallographic analysis of RNA polymerase holoenzyme from Thermus thermophilus."
Vassylyeva M.N., Lee J., Sekine S.I., Laptenko O., Kuramitsu S., Shibata T., Inoue Y., Borukhov S., Vassylyev D.G., Yokoyama S.
Acta Crystallogr. D 58:1497-1500(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete genome sequence of Thermus thermophilus HB8."
Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HB8 / ATCC 27634 / DSM 579.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AB083789 Genomic DNA. Translation: BAB89400.1.
AP008226 Genomic DNA. Translation: BAD71636.1.
RefSeqYP_145079.1. NC_006461.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1IW7X-ray2.60C/M1-1119[»]
1SMYX-ray2.70C/M1-1119[»]
1ZYRX-ray3.00C/M1-1119[»]
2A68X-ray2.50C/M1-1119[»]
2A69X-ray2.50C/M1-1119[»]
2A6EX-ray2.80C/M1-1119[»]
2A6HX-ray2.40C/M1-1119[»]
2BE5X-ray2.40C/M1-1119[»]
2CW0X-ray3.30C/M1-1119[»]
2O5IX-ray2.50C/M1-1119[»]
2O5JX-ray3.00C/M1-1119[»]
2PPBX-ray3.00C/M1-1119[»]
3AOHX-ray4.10C/H/M1-1119[»]
3AOIX-ray4.30C/H/M1-1119[»]
3DXJX-ray3.00C/M1-1119[»]
3EQLX-ray2.70C/M1-1119[»]
4G7HX-ray2.90C/M1-1119[»]
4G7OX-ray2.99C/M1-1119[»]
4G7ZX-ray3.82C/M1-1119[»]
4GZYX-ray3.51C1-1119[»]
4GZZX-ray4.29C1-1119[»]
ProteinModelPortalQ8RQE9.
SMRQ8RQE9. Positions 1-1119.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-47011N.
IntActQ8RQE9. 2 interactions.
STRING300852.TTHA1813.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD71636; BAD71636; BAD71636.
GeneID3169736.
KEGGttj:TTHA1813.
PATRIC23958595. VBITheThe93045_1784.

Phylogenomic databases

eggNOGCOG0085.
HOGENOMHOG000218611.
KOK03043.
OMAIGYIYML.
ProtClustDBPRK00405.

Family and domain databases

Gene3D2.30.150.10. 1 hit.
2.40.270.10. 2 hits.
2.40.50.150. 1 hit.
HAMAPMF_01321. RNApol_bact_RpoB.
InterProIPR010243. DNA-dir_RNA_pol_bsu.
IPR019462. DNA-dir_RNA_pol_bsu_external_1.
IPR015712. DNA-dir_RNA_pol_su2.
IPR007120. DNA-dir_RNA_pol_su2_6.
IPR007121. RNA_pol_bsu_CS.
IPR007644. RNA_pol_bsu_protrusion.
IPR007642. RNA_pol_Rpb2_2.
IPR007645. RNA_pol_Rpb2_3.
IPR007641. RNA_pol_Rpb2_7.
IPR014724. RNA_pol_RPB2_OB-fold.
[Graphical view]
PANTHERPTHR20856. PTHR20856. 1 hit.
PTHR20856:SF3. PTHR20856:SF3. 1 hit.
PfamPF04563. RNA_pol_Rpb2_1. 1 hit.
PF04561. RNA_pol_Rpb2_2. 1 hit.
PF04565. RNA_pol_Rpb2_3. 1 hit.
PF10385. RNA_pol_Rpb2_45. 1 hit.
PF00562. RNA_pol_Rpb2_6. 1 hit.
PF04560. RNA_pol_Rpb2_7. 1 hit.
[Graphical view]
TIGRFAMsTIGR02013. rpoB. 1 hit.
PROSITEPS01166. RNA_POL_BETA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ8RQE9.

Entry information

Entry nameRPOB_THET8
AccessionPrimary (citable) accession number: Q8RQE9
Secondary accession number(s): Q5SHB7
Entry history
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: June 1, 2002
Last modified: May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents