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Q8RQE9

- RPOB_THET8

UniProt

Q8RQE9 - RPOB_THET8

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Protein

DNA-directed RNA polymerase subunit beta

Gene

rpoB

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).UniRule annotation

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-HAMAP
  2. DNA-directed RNA polymerase activity Source: UniProtKB-HAMAP
  3. ribonucleoside binding Source: InterPro

GO - Biological processi

  1. transcription, DNA-templated Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Transcription

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-1858-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase subunit betaUniRule annotation (EC:2.7.7.6UniRule annotation)
Short name:
RNAP subunit betaUniRule annotation
Alternative name(s):
RNA polymerase subunit betaUniRule annotation
Transcriptase subunit betaUniRule annotation
Gene namesi
Name:rpoBUniRule annotation
Ordered Locus Names:TTHA1813
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
ProteomesiUP000000532: Chromosome

Subcellular locationi

Keywords - Cellular componenti

DNA-directed RNA polymerase

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11191119DNA-directed RNA polymerase subunit betaPRO_0000047985Add
BLAST

Interactioni

Subunit structurei

The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.

Protein-protein interaction databases

DIPiDIP-47011N.
IntActiQ8RQE9. 3 interactions.
STRINGi300852.TTHA1813.

Structurei

Secondary structure

1
1119
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 54Combined sources
Helixi20 – 2910Combined sources
Helixi31 – 333Combined sources
Helixi36 – 383Combined sources
Helixi43 – 519Combined sources
Beta strandi53 – 564Combined sources
Beta strandi59 – 613Combined sources
Beta strandi64 – 685Combined sources
Helixi80 – 856Combined sources
Beta strandi91 – 944Combined sources
Beta strandi98 – 1003Combined sources
Beta strandi103 – 1053Combined sources
Beta strandi107 – 1093Combined sources
Beta strandi117 – 1193Combined sources
Beta strandi123 – 1253Combined sources
Beta strandi129 – 1324Combined sources
Beta strandi134 – 1363Combined sources
Beta strandi138 – 1425Combined sources
Beta strandi146 – 1494Combined sources
Beta strandi152 – 1565Combined sources
Beta strandi159 – 1624Combined sources
Beta strandi166 – 1694Combined sources
Beta strandi172 – 1776Combined sources
Turni178 – 1803Combined sources
Beta strandi181 – 19111Combined sources
Helixi193 – 2008Combined sources
Helixi204 – 2129Combined sources
Turni215 – 2173Combined sources
Helixi218 – 2214Combined sources
Helixi225 – 2284Combined sources
Helixi231 – 24111Combined sources
Beta strandi242 – 2443Combined sources
Turni250 – 2523Combined sources
Helixi253 – 2575Combined sources
Beta strandi259 – 2646Combined sources
Turni269 – 2713Combined sources
Helixi274 – 2785Combined sources
Beta strandi281 – 2833Combined sources
Turni287 – 2904Combined sources
Beta strandi294 – 2963Combined sources
Beta strandi297 – 3026Combined sources
Helixi303 – 31412Combined sources
Beta strandi325 – 3273Combined sources
Turni328 – 3303Combined sources
Beta strandi331 – 3344Combined sources
Helixi336 – 36126Combined sources
Beta strandi364 – 3663Combined sources
Turni370 – 3723Combined sources
Helixi376 – 38712Combined sources
Beta strandi390 – 3945Combined sources
Helixi401 – 4088Combined sources
Beta strandi409 – 41810Combined sources
Turni420 – 4223Combined sources
Helixi425 – 4284Combined sources
Helixi432 – 4343Combined sources
Turni435 – 4373Combined sources
Beta strandi440 – 4434Combined sources
Beta strandi445 – 4484Combined sources
Beta strandi450 – 4556Combined sources
Beta strandi456 – 4583Combined sources
Beta strandi460 – 4623Combined sources
Beta strandi463 – 4653Combined sources
Beta strandi467 – 4759Combined sources
Beta strandi478 – 4869Combined sources
Helixi488 – 4936Combined sources
Beta strandi494 – 4974Combined sources
Beta strandi499 – 5013Combined sources
Beta strandi503 – 5086Combined sources
Beta strandi511 – 52414Combined sources
Turni526 – 5283Combined sources
Beta strandi531 – 5333Combined sources
Turni536 – 5394Combined sources
Helixi542 – 5454Combined sources
Helixi550 – 5523Combined sources
Helixi555 – 56511Combined sources
Beta strandi571 – 5733Combined sources
Beta strandi578 – 5803Combined sources
Helixi584 – 5907Combined sources
Beta strandi594 – 5963Combined sources
Beta strandi598 – 6069Combined sources
Beta strandi608 – 6158Combined sources
Turni616 – 6183Combined sources
Beta strandi619 – 6213Combined sources
Beta strandi628 – 6303Combined sources
Beta strandi634 – 6374Combined sources
Beta strandi654 – 6574Combined sources
Beta strandi661 – 6666Combined sources
Beta strandi669 – 6757Combined sources
Turni680 – 6834Combined sources
Beta strandi684 – 6918Combined sources
Helixi693 – 6964Combined sources
Turni697 – 7004Combined sources
Beta strandi703 – 71311Combined sources
Beta strandi727 – 7293Combined sources
Helixi731 – 7344Combined sources
Helixi735 – 7373Combined sources
Turni738 – 7403Combined sources
Beta strandi741 – 7433Combined sources
Beta strandi754 – 7563Combined sources
Beta strandi758 – 7614Combined sources
Beta strandi762 – 7665Combined sources
Helixi769 – 77810Combined sources
Beta strandi785 – 7873Combined sources
Beta strandi798 – 80710Combined sources
Beta strandi808 – 8103Combined sources
Beta strandi819 – 82911Combined sources
Beta strandi838 – 8403Combined sources
Beta strandi842 – 8443Combined sources
Beta strandi846 – 8538Combined sources
Turni855 – 8573Combined sources
Beta strandi862 – 8643Combined sources
Beta strandi868 – 8703Combined sources
Turni874 – 8807Combined sources
Helixi883 – 89614Combined sources
Beta strandi899 – 9024Combined sources
Turni905 – 9073Combined sources
Helixi911 – 93222Combined sources
Helixi938 – 94811Combined sources
Turni949 – 9513Combined sources
Beta strandi955 – 9573Combined sources
Helixi959 – 96810Combined sources
Beta strandi977 – 9793Combined sources
Beta strandi980 – 9823Combined sources
Beta strandi987 – 99711Combined sources
Turni1001 – 10033Combined sources
Beta strandi1006 – 10105Combined sources
Beta strandi1015 – 10173Combined sources
Beta strandi1024 – 10263Combined sources
Beta strandi1030 – 10323Combined sources
Helixi1034 – 10429Combined sources
Helixi1047 – 10537Combined sources
Turni1054 – 10585Combined sources
Helixi1060 – 107112Combined sources
Helixi1083 – 109412Combined sources
Beta strandi1098 – 11025Combined sources
Beta strandi1104 – 11063Combined sources
Beta strandi1114 – 11174Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1IW7X-ray2.60C/M1-1119[»]
1SMYX-ray2.70C/M1-1119[»]
1ZYRX-ray3.00C/M1-1119[»]
2A68X-ray2.50C/M1-1119[»]
2A69X-ray2.50C/M1-1119[»]
2A6EX-ray2.80C/M1-1119[»]
2A6HX-ray2.40C/M1-1119[»]
2BE5X-ray2.40C/M1-1119[»]
2CW0X-ray3.30C/M1-1119[»]
2O5IX-ray2.50C/M1-1119[»]
2O5JX-ray3.00C/M1-1119[»]
2PPBX-ray3.00C/M1-1119[»]
3AOHX-ray4.10C/H/M1-1119[»]
3AOIX-ray4.30C/H/M1-1119[»]
3DXJX-ray3.00C/M1-1119[»]
3EQLX-ray2.70C/M1-1119[»]
3WODX-ray3.60C1-1119[»]
3WOEX-ray2.35A/C703-830[»]
3WOFX-ray3.30A/C/E/G/I/K/M/O/Q/S/U/W703-830[»]
4G7HX-ray2.90C/M1-1119[»]
4G7OX-ray2.99C/M1-1119[»]
4G7ZX-ray3.82C/M1-1119[»]
4GZYX-ray3.51C1-1119[»]
4GZZX-ray4.29C1-1119[»]
4MQ9X-ray3.35C1-1119[»]
4OINX-ray2.80C1-1119[»]
4OIOX-ray3.10C1-1119[»]
4OIPX-ray3.40C1-1119[»]
4OIQX-ray3.62C1-1119[»]
4OIRX-ray3.10C1-1119[»]
4Q4ZX-ray2.90C1-1119[»]
4Q5SX-ray3.00C1-1119[»]
ProteinModelPortaliQ8RQE9.
SMRiQ8RQE9. Positions 1-1119.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8RQE9.

Family & Domainsi

Sequence similaritiesi

Belongs to the RNA polymerase beta chain family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0085.
HOGENOMiHOG000218611.
KOiK03043.
OMAiFPITDFS.
OrthoDBiEOG6M9DS6.
PhylomeDBiQ8RQE9.

Family and domain databases

Gene3Di2.30.150.10. 1 hit.
2.40.270.10. 2 hits.
2.40.50.150. 1 hit.
3.90.1110.10. 1 hit.
HAMAPiMF_01321. RNApol_bact_RpoB.
InterProiIPR010243. DNA-dir_RNA_pol_bsu.
IPR019462. DNA-dir_RNA_pol_bsu_external_1.
IPR015712. DNA-dir_RNA_pol_su2.
IPR007120. DNA-dir_RNA_pol_su2_6.
IPR007121. RNA_pol_bsu_CS.
IPR007644. RNA_pol_bsu_protrusion.
IPR007642. RNA_pol_Rpb2_2.
IPR007645. RNA_pol_Rpb2_3.
IPR007641. RNA_pol_Rpb2_7.
IPR014724. RNA_pol_RPB2_OB-fold.
[Graphical view]
PANTHERiPTHR20856. PTHR20856. 1 hit.
PfamiPF04563. RNA_pol_Rpb2_1. 1 hit.
PF04561. RNA_pol_Rpb2_2. 1 hit.
PF04565. RNA_pol_Rpb2_3. 1 hit.
PF10385. RNA_pol_Rpb2_45. 1 hit.
PF00562. RNA_pol_Rpb2_6. 1 hit.
PF04560. RNA_pol_Rpb2_7. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02013. rpoB. 1 hit.
PROSITEiPS01166. RNA_POL_BETA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8RQE9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEIKRFGRIR EVIPLPPLTE IQVESYRRAL QADVPPEKRE NVGIQAAFRE
60 70 80 90 100
TFPIEEEDKG KGGLVLDFLE YRLGEPPFPQ DECREKDLTY QAPLYARLQL
110 120 130 140 150
IHKDTGLIKE DEVFLGHIPL MTEDGSFIIN GADRVIVSQI HRSPGVYFTP
160 170 180 190 200
DPARPGRYIA SIIPLPKRGP WIDLEVEPNG VVSMKVNKRK FPLVLLLRVL
210 220 230 240 250
GYDQETLARE LGAYGELVQG LMDESVFAMR PEEALIRLFT LLRPGDPPKR
260 270 280 290 300
DKAVAYVYGL IADPRRYDLG EAGRYKAEEK LGIRLSGRTL ARFEDGEFKD
310 320 330 340 350
EVFLPTLRYL FALTAGVPGH EVDDIDHLGN RRIRTVGELM TDQFRVGLAR
360 370 380 390 400
LARGVRERML MGSEDSLTPA KLVNSRPLEA AIREFFSRSQ LSQFKDETNP
410 420 430 440 450
LSSLRHKRRI SALGPGGLTR ERAGFDVRDV HRTHYGRICP VETPEGANIG
460 470 480 490 500
LITSLAAYAR VDELGFIRTP YRRVVGGVVT DEVVYMTATE EDRYTIAQAN
510 520 530 540 550
TPLEGNRIAA ERVVARRKGE PVIVSPEEVE FMDVSPKQVF SVNTNLIPFL
560 570 580 590 600
EHDDANRALM GSNMQTQAVP LIRAQAPVVM TGLEERVVRD SLAALYAEED
610 620 630 640 650
GEVAKVDGNR IVVRYEDGRL VEYPLRRFYR SNQGTALDQR PRVVVGQRVR
660 670 680 690 700
KGDLLADGPA SENGFLALGQ NVLVAIMPFD GYNFEDAIVI SEELLKRDFY
710 720 730 740 750
TSIHIERYEI EARDTKLGPE RITRDIPHLS EAALRDLDEE GVVRIGAEVK
760 770 780 790 800
PGDILVGRTS FKGESEPTPE ERLLRSIFGE KARDVKDTSL RVPPGEGGIV
810 820 830 840 850
VRTVRLRRGD PGVELKPGVR EVVRVYVAQK RKLQVGDKLA NRHGNKGVVA
860 870 880 890 900
KILPVEDMPH LPDGTPVDVI LNPLGVPSRM NLGQILETHL GLAGYFLGQR
910 920 930 940 950
YISPIFDGAK EPEIKELLAQ AFEVYFGKRK GEGFGVDKRE VEVLRRAEKL
960 970 980 990 1000
GLVTPGKTPE EQLKELFLQG KVVLYDGRTG EPIEGPIVVG QMFIMKLYHM
1010 1020 1030 1040 1050
VEDKMHARST GPYSLITQQP LGGKAQFGGQ RFGEMEVWAL EAYGAAHTLQ
1060 1070 1080 1090 1100
EMLTLKSDDI EGRNAAYEAI IKGEDVPEPS VPESFRVLVK ELQALALDVQ
1110
TLDEKDNPVD IFEGLASKR
Length:1,119
Mass (Da):125,264
Last modified:June 1, 2002 - v1
Checksum:iC1260F31CEC6B40D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB083789 Genomic DNA. Translation: BAB89400.1.
AP008226 Genomic DNA. Translation: BAD71636.1.
RefSeqiWP_011228935.1. NC_006461.1.
YP_145079.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD71636; BAD71636; BAD71636.
GeneIDi3169736.
KEGGittj:TTHA1813.
PATRICi23958595. VBITheThe93045_1784.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB083789 Genomic DNA. Translation: BAB89400.1 .
AP008226 Genomic DNA. Translation: BAD71636.1 .
RefSeqi WP_011228935.1. NC_006461.1.
YP_145079.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1IW7 X-ray 2.60 C/M 1-1119 [» ]
1SMY X-ray 2.70 C/M 1-1119 [» ]
1ZYR X-ray 3.00 C/M 1-1119 [» ]
2A68 X-ray 2.50 C/M 1-1119 [» ]
2A69 X-ray 2.50 C/M 1-1119 [» ]
2A6E X-ray 2.80 C/M 1-1119 [» ]
2A6H X-ray 2.40 C/M 1-1119 [» ]
2BE5 X-ray 2.40 C/M 1-1119 [» ]
2CW0 X-ray 3.30 C/M 1-1119 [» ]
2O5I X-ray 2.50 C/M 1-1119 [» ]
2O5J X-ray 3.00 C/M 1-1119 [» ]
2PPB X-ray 3.00 C/M 1-1119 [» ]
3AOH X-ray 4.10 C/H/M 1-1119 [» ]
3AOI X-ray 4.30 C/H/M 1-1119 [» ]
3DXJ X-ray 3.00 C/M 1-1119 [» ]
3EQL X-ray 2.70 C/M 1-1119 [» ]
3WOD X-ray 3.60 C 1-1119 [» ]
3WOE X-ray 2.35 A/C 703-830 [» ]
3WOF X-ray 3.30 A/C/E/G/I/K/M/O/Q/S/U/W 703-830 [» ]
4G7H X-ray 2.90 C/M 1-1119 [» ]
4G7O X-ray 2.99 C/M 1-1119 [» ]
4G7Z X-ray 3.82 C/M 1-1119 [» ]
4GZY X-ray 3.51 C 1-1119 [» ]
4GZZ X-ray 4.29 C 1-1119 [» ]
4MQ9 X-ray 3.35 C 1-1119 [» ]
4OIN X-ray 2.80 C 1-1119 [» ]
4OIO X-ray 3.10 C 1-1119 [» ]
4OIP X-ray 3.40 C 1-1119 [» ]
4OIQ X-ray 3.62 C 1-1119 [» ]
4OIR X-ray 3.10 C 1-1119 [» ]
4Q4Z X-ray 2.90 C 1-1119 [» ]
4Q5S X-ray 3.00 C 1-1119 [» ]
ProteinModelPortali Q8RQE9.
SMRi Q8RQE9. Positions 1-1119.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-47011N.
IntActi Q8RQE9. 3 interactions.
STRINGi 300852.TTHA1813.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAD71636 ; BAD71636 ; BAD71636 .
GeneIDi 3169736.
KEGGi ttj:TTHA1813.
PATRICi 23958595. VBITheThe93045_1784.

Phylogenomic databases

eggNOGi COG0085.
HOGENOMi HOG000218611.
KOi K03043.
OMAi FPITDFS.
OrthoDBi EOG6M9DS6.
PhylomeDBi Q8RQE9.

Enzyme and pathway databases

BioCyci TTHE300852:GH8R-1858-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q8RQE9.

Family and domain databases

Gene3Di 2.30.150.10. 1 hit.
2.40.270.10. 2 hits.
2.40.50.150. 1 hit.
3.90.1110.10. 1 hit.
HAMAPi MF_01321. RNApol_bact_RpoB.
InterProi IPR010243. DNA-dir_RNA_pol_bsu.
IPR019462. DNA-dir_RNA_pol_bsu_external_1.
IPR015712. DNA-dir_RNA_pol_su2.
IPR007120. DNA-dir_RNA_pol_su2_6.
IPR007121. RNA_pol_bsu_CS.
IPR007644. RNA_pol_bsu_protrusion.
IPR007642. RNA_pol_Rpb2_2.
IPR007645. RNA_pol_Rpb2_3.
IPR007641. RNA_pol_Rpb2_7.
IPR014724. RNA_pol_RPB2_OB-fold.
[Graphical view ]
PANTHERi PTHR20856. PTHR20856. 1 hit.
Pfami PF04563. RNA_pol_Rpb2_1. 1 hit.
PF04561. RNA_pol_Rpb2_2. 1 hit.
PF04565. RNA_pol_Rpb2_3. 1 hit.
PF10385. RNA_pol_Rpb2_45. 1 hit.
PF00562. RNA_pol_Rpb2_6. 1 hit.
PF04560. RNA_pol_Rpb2_7. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR02013. rpoB. 1 hit.
PROSITEi PS01166. RNA_POL_BETA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Purification, crystallization and initial crystallographic analysis of RNA polymerase holoenzyme from Thermus thermophilus."
    Vassylyeva M.N., Lee J., Sekine S.I., Laptenko O., Kuramitsu S., Shibata T., Inoue Y., Borukhov S., Vassylyev D.G., Yokoyama S.
    Acta Crystallogr. D 58:1497-1500(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete genome sequence of Thermus thermophilus HB8."
    Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HB8 / ATCC 27634 / DSM 579.

Entry informationi

Entry nameiRPOB_THET8
AccessioniPrimary (citable) accession number: Q8RQE9
Secondary accession number(s): Q5SHB7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: June 1, 2002
Last modified: November 26, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3