DNA-directed RNA polymerase subunit beta' - Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)

Sequence or UniProt identifier

>sp|Q8RQE8|RPOC_THET8 DNA-directed RNA polymerase subunit beta' OS=Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) GN=rpoC PE=1 SV=1 MKKEVRKVRIALASPEKIRSWSYGEVEKPETINYRTLKPERDGLFDERIFGPIKDYECAC GKYKRQRFEGKVCERCGVEVTKSIVRRYRMGHIELATPAAHIWFVKDVPSKIGTLLDLSA TELEQVLYFSKYIVLDPKGAILNGVPVEKRQLLTDEEYRELRYGKQETYPLPPGVDALVK DGEEVVKGQELAPGVVSRLDGVALYRFPRRVRVEYVKKERAGLRLPLAAWVEKEAYKPGE ILAELPEPYLFRAEEEGVVELKELEEGAFLVLRREDEPVATYFLPVGMTPLVVHGEIVEK GQPLAEAKGLLRMPRQVRAAQVEAEEEGETVYLTLFLEWTEPKDYRVQPHMNVVVPEGAR VEAGDKIVAAIDPEEEVIAEAEGVVHLHEPASILVVKARVYPFEDDVEVSTGDRVAPGDV LADGGKVKSDVYGRVEVDLVRNVVRVVESYDIDARMGAEAIQQLLKELDLEALEKELLEE MKHPSRARRAKARKRLEVVRAFLDSGNRPEWMILEAVPVLPPDLRPMVQVDGGRFATSDL NDLYRRLINRNNRLKKLLAQGAPEIIIRNEKRMLQEAVDALLDNGRRGAPVTNPGSDRPL RSLTDILSGKQGRFRQNLLGKRVDYSGRSVIVVGPQLKLHQCGLPKRMALELFKPFLLKK MEEKGIAPNVKAARRMLERQRDIKDEVWDALEEVIHGKVVLLNRAPTLHRLGIQAFQPVL VEGQSIQLHPLVCEAFNADFDGDQMAVHVPLSSFAQAEARIQMLSAHNLLSPASGEPLAK PSRDIILGLYYITQVRKEKKGAGLEFATPEEALAAHERGEVALNAPIKVAGRETSVGRLK YVFANPDEALLAVAHGIVDLQDVVTVRYMGKRLETSPGRILFARIVAEAVEDEKVAWELI QLDVPQEKNSLKDLVYQAFLRLGMEKTARLLDALKYYGFTFSTTSGITIGIDDAVIPEEK KQYLEEADRKLLQIEQAYEMGFLTDRERYDQILQLWTETTEKVTQAVFKNFEENYPFNPL YVMAQSGARGNPQQIRQLCGLRGLMQKPSGETFEVPVRSSFREGLTVLEYFISSHGARKG GADTALRTADSGYLTRKLVDVTHEIVVREADCGTTNYISVPLFQPDEVTRSLRLRKRADI EAGLYGRVLAREVEVLGVRLEEGRYLSMDDVHLLIKAAEAGEIQEVPVRSPLTCQTRYGV CQKCYGYDLSMARPVSIGEAVGIVAAQSIGEPGTQLTMRTFHTGGVAGAADITQGLPRVI ELFEARRPKAKAVISEIDGVVRIEETEEKLSVFVESEGFSKEYKLPKEARLLVKDGDYVE AGQPLTRGAIDPHQLLEAKGPEAVERYLVEEIQKVYRAQGVKLHDKHIEIVVRQMMKYVE VTDPGDSRLLEGQVLEKWDVEALNERLIAEGKTPVAWKPLLMGVTKSALSTKSWLSAASF QNTTHVLTEAAIAGKKDELIGLKENVILGRLIPAGTGSDFVRFTQVVDQKTLKAIEEARK EAVEAKERPAARRGVKREQPGKQA

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Q8RQE8 (RPOC_THET8) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 86. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit beta'
Short name=RNAP subunit beta'
EC=2.7.7.6

Alternative name(s):
RNA polymerase subunit beta'
Transcriptase subunit beta'

Gene names

Name:	rpoC
Ordered Locus Names:	TTHA1812

Organism

Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Reference proteome] [HAMAP]

Taxonomic identifier

300852 [NCBI]

Taxonomic lineage

Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus ›

Protein attributes

Sequence length	1524 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. HAMAP-Rule MF_01322
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP-Rule MF_01322
Subunit structure	The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.
Sequence similarities	Belongs to the RNA polymerase beta' chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	transcription, DNA-dependent Inferred from electronic annotation. Source: HAMAP
Molecular_function	DNA binding Inferred from electronic annotation. Source: HAMAP DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1524

1524

DNA-directed RNA polymerase subunit beta' HAMAP-Rule MF_01322

PRO_0000067820

Secondary structure

1524

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

Q8RQE8 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: A7B335945B08E3A7
Show »

FASTA

1,524

170,756

        10         20         30         40         50         60 
MKKEVRKVRI ALASPEKIRS WSYGEVEKPE TINYRTLKPE RDGLFDERIF GPIKDYECAC 

        70         80         90        100        110        120 
GKYKRQRFEG KVCERCGVEV TKSIVRRYRM GHIELATPAA HIWFVKDVPS KIGTLLDLSA 

       130        140        150        160        170        180 
TELEQVLYFS KYIVLDPKGA ILNGVPVEKR QLLTDEEYRE LRYGKQETYP LPPGVDALVK 

       190        200        210        220        230        240 
DGEEVVKGQE LAPGVVSRLD GVALYRFPRR VRVEYVKKER AGLRLPLAAW VEKEAYKPGE 

       250        260        270        280        290        300 
ILAELPEPYL FRAEEEGVVE LKELEEGAFL VLRREDEPVA TYFLPVGMTP LVVHGEIVEK 

       310        320        330        340        350        360 
GQPLAEAKGL LRMPRQVRAA QVEAEEEGET VYLTLFLEWT EPKDYRVQPH MNVVVPEGAR 

       370        380        390        400        410        420 
VEAGDKIVAA IDPEEEVIAE AEGVVHLHEP ASILVVKARV YPFEDDVEVS TGDRVAPGDV 

       430        440        450        460        470        480 
LADGGKVKSD VYGRVEVDLV RNVVRVVESY DIDARMGAEA IQQLLKELDL EALEKELLEE 

       490        500        510        520        530        540 
MKHPSRARRA KARKRLEVVR AFLDSGNRPE WMILEAVPVL PPDLRPMVQV DGGRFATSDL 

       550        560        570        580        590        600 
NDLYRRLINR NNRLKKLLAQ GAPEIIIRNE KRMLQEAVDA LLDNGRRGAP VTNPGSDRPL 

       610        620        630        640        650        660 
RSLTDILSGK QGRFRQNLLG KRVDYSGRSV IVVGPQLKLH QCGLPKRMAL ELFKPFLLKK 

       670        680        690        700        710        720 
MEEKGIAPNV KAARRMLERQ RDIKDEVWDA LEEVIHGKVV LLNRAPTLHR LGIQAFQPVL 

       730        740        750        760        770        780 
VEGQSIQLHP LVCEAFNADF DGDQMAVHVP LSSFAQAEAR IQMLSAHNLL SPASGEPLAK 

       790        800        810        820        830        840 
PSRDIILGLY YITQVRKEKK GAGLEFATPE EALAAHERGE VALNAPIKVA GRETSVGRLK 

       850        860        870        880        890        900 
YVFANPDEAL LAVAHGIVDL QDVVTVRYMG KRLETSPGRI LFARIVAEAV EDEKVAWELI 

       910        920        930        940        950        960 
QLDVPQEKNS LKDLVYQAFL RLGMEKTARL LDALKYYGFT FSTTSGITIG IDDAVIPEEK 

       970        980        990       1000       1010       1020 
KQYLEEADRK LLQIEQAYEM GFLTDRERYD QILQLWTETT EKVTQAVFKN FEENYPFNPL 

      1030       1040       1050       1060       1070       1080 
YVMAQSGARG NPQQIRQLCG LRGLMQKPSG ETFEVPVRSS FREGLTVLEY FISSHGARKG 

      1090       1100       1110       1120       1130       1140 
GADTALRTAD SGYLTRKLVD VTHEIVVREA DCGTTNYISV PLFQPDEVTR SLRLRKRADI 

      1150       1160       1170       1180       1190       1200 
EAGLYGRVLA REVEVLGVRL EEGRYLSMDD VHLLIKAAEA GEIQEVPVRS PLTCQTRYGV 

      1210       1220       1230       1240       1250       1260 
CQKCYGYDLS MARPVSIGEA VGIVAAQSIG EPGTQLTMRT FHTGGVAGAA DITQGLPRVI 

      1270       1280       1290       1300       1310       1320 
ELFEARRPKA KAVISEIDGV VRIEETEEKL SVFVESEGFS KEYKLPKEAR LLVKDGDYVE 

      1330       1340       1350       1360       1370       1380 
AGQPLTRGAI DPHQLLEAKG PEAVERYLVE EIQKVYRAQG VKLHDKHIEI VVRQMMKYVE 

      1390       1400       1410       1420       1430       1440 
VTDPGDSRLL EGQVLEKWDV EALNERLIAE GKTPVAWKPL LMGVTKSALS TKSWLSAASF 

      1450       1460       1470       1480       1490       1500 
QNTTHVLTEA AIAGKKDELI GLKENVILGR LIPAGTGSDF VRFTQVVDQK TLKAIEEARK 

      1510       1520 
EAVEAKERPA ARRGVKREQP GKQA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Purification, crystallization and initial crystallographic analysis of RNA polymerase holoenzyme from Thermus thermophilus." Vassylyeva M.N., Lee J., Sekine S.I., Laptenko O., Kuramitsu S., Shibata T., Inoue Y., Borukhov S., Vassylyev D.G., Yokoyama S. Acta Crystallogr. D 58:1497-1500(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete genome sequence of Thermus thermophilus HB8." Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S. Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HB8 / ATCC 27634 / DSM 579.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB083790 Genomic DNA. Translation: BAB89401.1.
AP008226 Genomic DNA. Translation: BAD71635.1.

RefSeq

YP_145078.1. NC_006461.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1IW7	X-ray	2.60	D/N	1-1524	[»]
1SMY	X-ray	2.70	D/N	1-1524	[»]
1ZYR	X-ray	3.00	D/N	1-1524	[»]
2A68	X-ray	2.50	D/N	1-1524	[»]
2A69	X-ray	2.50	D/N	1-1524	[»]
2A6E	X-ray	2.80	D/N	1-1524	[»]
2A6H	X-ray	2.40	D/N	1-1524	[»]
2BE5	X-ray	2.40	D/N	1-1524	[»]
2CW0	X-ray	3.30	D/N	1-1524	[»]
2O5I	X-ray	2.50	D/N	1-1524	[»]
2O5J	X-ray	3.00	D/N	1-1524	[»]
2PPB	X-ray	3.00	D/N	1-1524	[»]
3AOH	X-ray	4.10	D/I/N	1-1524	[»]
3AOI	X-ray	4.30	D/I/N	1-1524	[»]
3DXJ	X-ray	3.00	D/N	1-1524	[»]
3EQL	X-ray	2.70	D/N	1-1524	[»]
4G7H	X-ray	2.90	D/N	1-1524	[»]
4G7O	X-ray	2.99	D/N	1-1524	[»]
4G7Z	X-ray	3.82	D/N	1-1524	[»]
4GZY	X-ray	3.51	D	1-1524	[»]
4GZZ	X-ray	4.29	D	1-1524	[»]

ProteinModelPortal

Q8RQE8.

SMR

Q8RQE8. Positions 2-207.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-47007N.

STRING

300852.TTHA1812.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

BAD71635; BAD71635; BAD71635.

GeneID

3169754.

KEGG

ttj:TTHA1812.

PATRIC

23958593. VBITheThe93045_1783.

Phylogenomic databases

eggNOG

COG0086.

HOGENOM

HOG000218387.

K03046.

OMA

DINDKHI.

ProtClustDB

PRK00566.

Enzyme and pathway databases

BioCyc

TTHE300852:GH8R-1857-MONOMER.

Family and domain databases

Gene3D

2.40.40.20. 2 hits.

HAMAP

MF_01322. RNApol_bact_RpoC.

InterPro

IPR009010. Asp_de-COase-like_dom.
IPR012754. DNA-dir_RpoC_beta_prime.
IPR000722. RNA_pol_asu.
IPR006592. RNA_pol_N.
IPR007080. RNA_pol_Rpb1_1.
IPR007066. RNA_pol_Rpb1_3.
IPR007083. RNA_pol_Rpb1_4.
IPR007081. RNA_pol_Rpb1_5.
[Graphical view]

Pfam

PF04997. RNA_pol_Rpb1_1. 2 hits.
PF00623. RNA_pol_Rpb1_2. 1 hit.
PF04983. RNA_pol_Rpb1_3. 1 hit.
PF05000. RNA_pol_Rpb1_4. 1 hit.
PF04998. RNA_pol_Rpb1_5. 1 hit.
[Graphical view]

SMART

SM00663. RPOLA_N. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q8RQE8.

Entry information

Entry name

RPOC_THET8

Accession

Primary (citable) accession number: Q8RQE8
Secondary accession number(s): Q5SHB8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	June 1, 2002
Last modified:	May 29, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Other

Entry information

Relevant documents