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Q8RQE8

- RPOC_THET8

UniProt

Q8RQE8 - RPOC_THET8

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Protein

DNA-directed RNA polymerase subunit beta'

Gene

rpoC

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).UniRule annotation

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-HAMAP
  2. DNA-directed RNA polymerase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. transcription, DNA-templated Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Transcription

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-1857-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase subunit beta'UniRule annotation (EC:2.7.7.6UniRule annotation)
Short name:
RNAP subunit beta'UniRule annotation
Alternative name(s):
RNA polymerase subunit beta'UniRule annotation
Transcriptase subunit beta'UniRule annotation
Gene namesi
Name:rpoCUniRule annotation
Ordered Locus Names:TTHA1812
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
ProteomesiUP000000532: Chromosome

Subcellular locationi

Keywords - Cellular componenti

DNA-directed RNA polymerase

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15241524DNA-directed RNA polymerase subunit beta'PRO_0000067820Add
BLAST

Interactioni

Subunit structurei

The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.

Protein-protein interaction databases

DIPiDIP-47007N.
IntActiQ8RQE8. 1 interaction.
STRINGi300852.TTHA1812.

Structurei

Secondary structure

1
1524
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 115Combined sources
Helixi15 – 217Combined sources
Beta strandi22 – 254Combined sources
Beta strandi36 – 394Combined sources
Beta strandi41 – 433Combined sources
Turni47 – 504Combined sources
Beta strandi53 – 564Combined sources
Beta strandi59 – 635Combined sources
Turni67 – 704Combined sources
Beta strandi74 – 763Combined sources
Beta strandi81 – 833Combined sources
Helixi84 – 885Combined sources
Beta strandi90 – 10011Combined sources
Helixi102 – 1054Combined sources
Beta strandi106 – 1094Combined sources
Helixi111 – 1155Combined sources
Helixi122 – 1298Combined sources
Beta strandi133 – 1353Combined sources
Beta strandi143 – 1464Combined sources
Helixi155 – 1628Combined sources
Beta strandi167 – 1704Combined sources
Beta strandi173 – 1764Combined sources
Helixi177 – 18610Combined sources
Beta strandi187 – 1893Combined sources
Beta strandi191 – 1944Combined sources
Beta strandi195 – 1973Combined sources
Beta strandi199 – 2013Combined sources
Beta strandi204 – 2063Combined sources
Beta strandi207 – 2093Combined sources
Beta strandi211 – 2133Combined sources
Beta strandi217 – 2226Combined sources
Turni226 – 2316Combined sources
Helixi233 – 2364Combined sources
Beta strandi237 – 2393Combined sources
Helixi241 – 2444Combined sources
Beta strandi245 – 2473Combined sources
Helixi248 – 2503Combined sources
Beta strandi257 – 2637Combined sources
Beta strandi268 – 28417Combined sources
Beta strandi286 – 2905Combined sources
Beta strandi297 – 3004Combined sources
Beta strandi303 – 31210Combined sources
Beta strandi315 – 32713Combined sources
Beta strandi330 – 34617Combined sources
Beta strandi359 – 3613Combined sources
Beta strandi366 – 3694Combined sources
Helixi373 – 3753Combined sources
Turni378 – 3814Combined sources
Beta strandi383 – 3875Combined sources
Beta strandi391 – 3944Combined sources
Beta strandi396 – 4016Combined sources
Beta strandi407 – 4093Combined sources
Beta strandi415 – 4173Combined sources
Beta strandi420 – 4223Combined sources
Beta strandi423 – 4253Combined sources
Beta strandi426 – 4283Combined sources
Beta strandi434 – 4385Combined sources
Turni439 – 4424Combined sources
Beta strandi443 – 4475Combined sources
Beta strandi452 – 4554Combined sources
Helixi458 – 46710Combined sources
Helixi470 – 48213Combined sources
Helixi486 – 50419Combined sources
Turni509 – 5124Combined sources
Beta strandi513 – 5197Combined sources
Turni522 – 5243Combined sources
Beta strandi527 – 5293Combined sources
Beta strandi531 – 5333Combined sources
Beta strandi535 – 5373Combined sources
Helixi540 – 56021Combined sources
Helixi564 – 58219Combined sources
Beta strandi584 – 5885Combined sources
Beta strandi593 – 5953Combined sources
Beta strandi596 – 5994Combined sources
Helixi603 – 6075Combined sources
Turni609 – 6113Combined sources
Helixi613 – 6197Combined sources
Beta strandi621 – 63313Combined sources
Beta strandi635 – 6373Combined sources
Beta strandi641 – 6455Combined sources
Helixi646 – 6527Combined sources
Helixi654 – 66310Combined sources
Beta strandi664 – 6663Combined sources
Beta strandi667 – 6693Combined sources
Helixi670 – 6778Combined sources
Helixi680 – 6823Combined sources
Helixi685 – 69410Combined sources
Turni695 – 6973Combined sources
Beta strandi700 – 7034Combined sources
Helixi710 – 7123Combined sources
Beta strandi713 – 72412Combined sources
Beta strandi726 – 7283Combined sources
Helixi731 – 7333Combined sources
Turni734 – 7374Combined sources
Turni740 – 7423Combined sources
Beta strandi744 – 7485Combined sources
Helixi753 – 76210Combined sources
Turni765 – 7673Combined sources
Beta strandi772 – 7798Combined sources
Helixi783 – 79311Combined sources
Beta strandi799 – 8013Combined sources
Beta strandi805 – 8084Combined sources
Helixi810 – 82112Combined sources
Beta strandi823 – 8253Combined sources
Beta strandi826 – 8294Combined sources
Beta strandi832 – 8354Combined sources
Helixi836 – 8405Combined sources
Beta strandi842 – 8454Combined sources
Helixi846 – 8549Combined sources
Beta strandi860 – 8623Combined sources
Beta strandi865 – 8673Combined sources
Beta strandi871 – 8733Combined sources
Helixi877 – 88913Combined sources
Helixi893 – 8986Combined sources
Helixi908 – 92114Combined sources
Helixi924 – 94522Combined sources
Turni951 – 9533Combined sources
Helixi960 – 97920Combined sources
Beta strandi980 – 9834Combined sources
Helixi985 – 101329Combined sources
Helixi1019 – 10257Combined sources
Beta strandi1026 – 10294Combined sources
Helixi1032 – 10387Combined sources
Beta strandi1048 – 10503Combined sources
Beta strandi1061 – 10633Combined sources
Helixi1068 – 110235Combined sources
Beta strandi1106 – 11105Combined sources
Beta strandi1118 – 11236Combined sources
Beta strandi1127 – 11304Combined sources
Beta strandi1131 – 11344Combined sources
Helixi1137 – 11448Combined sources
Beta strandi1148 – 11514Combined sources
Beta strandi1153 – 11553Combined sources
Beta strandi1156 – 11605Combined sources
Turni1168 – 11725Combined sources
Helixi1173 – 11808Combined sources
Beta strandi1184 – 11885Combined sources
Helixi1191 – 11933Combined sources
Beta strandi1197 – 12004Combined sources
Helixi1202 – 12054Combined sources
Beta strandi1209 – 12146Combined sources
Helixi1221 – 123010Combined sources
Helixi1232 – 12343Combined sources
Helixi1240 – 12434Combined sources
Beta strandi1246 – 12483Combined sources
Helixi1256 – 12638Combined sources
Beta strandi1269 – 12713Combined sources
Beta strandi1279 – 12835Combined sources
Beta strandi1290 – 12956Combined sources
Beta strandi1300 – 13056Combined sources
Beta strandi1317 – 13193Combined sources
Beta strandi1324 – 13296Combined sources
Helixi1332 – 13398Combined sources
Helixi1341 – 135717Combined sources
Turni1358 – 13603Combined sources
Helixi1365 – 137410Combined sources
Beta strandi1377 – 13837Combined sources
Beta strandi1385 – 13873Combined sources
Beta strandi1392 – 13943Combined sources
Helixi1395 – 140612Combined sources
Turni1409 – 14113Combined sources
Beta strandi1416 – 14194Combined sources
Helixi1424 – 14296Combined sources
Helixi1434 – 14396Combined sources
Helixi1443 – 145210Combined sources
Beta strandi1455 – 14584Combined sources
Helixi1462 – 14676Combined sources
Beta strandi1473 – 14753Combined sources
Turni1479 – 14813Combined sources
Beta strandi1485 – 14873Combined sources
Helixi1489 – 150012Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1IW7X-ray2.60D/N1-1524[»]
1SMYX-ray2.70D/N1-1524[»]
1ZYRX-ray3.00D/N1-1524[»]
2A68X-ray2.50D/N1-1524[»]
2A69X-ray2.50D/N1-1524[»]
2A6EX-ray2.80D/N1-1524[»]
2A6HX-ray2.40D/N1-1524[»]
2BE5X-ray2.40D/N1-1524[»]
2CW0X-ray3.30D/N1-1524[»]
2O5IX-ray2.50D/N1-1524[»]
2O5JX-ray3.00D/N1-1524[»]
2PPBX-ray3.00D/N1-1524[»]
3AOHX-ray4.10D/I/N1-1524[»]
3AOIX-ray4.30D/I/N1-1524[»]
3DXJX-ray3.00D/N1-1524[»]
3EQLX-ray2.70D/N1-1524[»]
3WODX-ray3.60D1-1524[»]
4G7HX-ray2.90D/N1-1524[»]
4G7OX-ray2.99D/N1-1524[»]
4G7ZX-ray3.82D/N1-1524[»]
4GZYX-ray3.51D1-1524[»]
4GZZX-ray4.29D1-1524[»]
4MQ9X-ray3.35D1-1524[»]
4OINX-ray2.80D1-1524[»]
4OIOX-ray3.10D1-1524[»]
4OIPX-ray3.40D1-1524[»]
4OIQX-ray3.62D1-1524[»]
4OIRX-ray3.10D1-1524[»]
4Q4ZX-ray2.90D1-1524[»]
4Q5SX-ray3.00D1-1524[»]
ProteinModelPortaliQ8RQE8.
SMRiQ8RQE8. Positions 2-207.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8RQE8.

Family & Domainsi

Sequence similaritiesi

Belongs to the RNA polymerase beta' chain family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0086.
HOGENOMiHOG000218387.
KOiK03046.
OMAiAYDSEFL.
OrthoDBiEOG6M9DS6.
PhylomeDBiQ8RQE8.

Family and domain databases

HAMAPiMF_01322. RNApol_bact_RpoC.
InterProiIPR012754. DNA-dir_RpoC_beta_prime.
IPR000722. RNA_pol_asu.
IPR006592. RNA_pol_N.
IPR007080. RNA_pol_Rpb1_1.
IPR007066. RNA_pol_Rpb1_3.
IPR007083. RNA_pol_Rpb1_4.
IPR007081. RNA_pol_Rpb1_5.
[Graphical view]
PfamiPF04997. RNA_pol_Rpb1_1. 2 hits.
PF00623. RNA_pol_Rpb1_2. 1 hit.
PF04983. RNA_pol_Rpb1_3. 1 hit.
PF05000. RNA_pol_Rpb1_4. 1 hit.
PF04998. RNA_pol_Rpb1_5. 1 hit.
[Graphical view]
SMARTiSM00663. RPOLA_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8RQE8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKKEVRKVRI ALASPEKIRS WSYGEVEKPE TINYRTLKPE RDGLFDERIF
60 70 80 90 100
GPIKDYECAC GKYKRQRFEG KVCERCGVEV TKSIVRRYRM GHIELATPAA
110 120 130 140 150
HIWFVKDVPS KIGTLLDLSA TELEQVLYFS KYIVLDPKGA ILNGVPVEKR
160 170 180 190 200
QLLTDEEYRE LRYGKQETYP LPPGVDALVK DGEEVVKGQE LAPGVVSRLD
210 220 230 240 250
GVALYRFPRR VRVEYVKKER AGLRLPLAAW VEKEAYKPGE ILAELPEPYL
260 270 280 290 300
FRAEEEGVVE LKELEEGAFL VLRREDEPVA TYFLPVGMTP LVVHGEIVEK
310 320 330 340 350
GQPLAEAKGL LRMPRQVRAA QVEAEEEGET VYLTLFLEWT EPKDYRVQPH
360 370 380 390 400
MNVVVPEGAR VEAGDKIVAA IDPEEEVIAE AEGVVHLHEP ASILVVKARV
410 420 430 440 450
YPFEDDVEVS TGDRVAPGDV LADGGKVKSD VYGRVEVDLV RNVVRVVESY
460 470 480 490 500
DIDARMGAEA IQQLLKELDL EALEKELLEE MKHPSRARRA KARKRLEVVR
510 520 530 540 550
AFLDSGNRPE WMILEAVPVL PPDLRPMVQV DGGRFATSDL NDLYRRLINR
560 570 580 590 600
NNRLKKLLAQ GAPEIIIRNE KRMLQEAVDA LLDNGRRGAP VTNPGSDRPL
610 620 630 640 650
RSLTDILSGK QGRFRQNLLG KRVDYSGRSV IVVGPQLKLH QCGLPKRMAL
660 670 680 690 700
ELFKPFLLKK MEEKGIAPNV KAARRMLERQ RDIKDEVWDA LEEVIHGKVV
710 720 730 740 750
LLNRAPTLHR LGIQAFQPVL VEGQSIQLHP LVCEAFNADF DGDQMAVHVP
760 770 780 790 800
LSSFAQAEAR IQMLSAHNLL SPASGEPLAK PSRDIILGLY YITQVRKEKK
810 820 830 840 850
GAGLEFATPE EALAAHERGE VALNAPIKVA GRETSVGRLK YVFANPDEAL
860 870 880 890 900
LAVAHGIVDL QDVVTVRYMG KRLETSPGRI LFARIVAEAV EDEKVAWELI
910 920 930 940 950
QLDVPQEKNS LKDLVYQAFL RLGMEKTARL LDALKYYGFT FSTTSGITIG
960 970 980 990 1000
IDDAVIPEEK KQYLEEADRK LLQIEQAYEM GFLTDRERYD QILQLWTETT
1010 1020 1030 1040 1050
EKVTQAVFKN FEENYPFNPL YVMAQSGARG NPQQIRQLCG LRGLMQKPSG
1060 1070 1080 1090 1100
ETFEVPVRSS FREGLTVLEY FISSHGARKG GADTALRTAD SGYLTRKLVD
1110 1120 1130 1140 1150
VTHEIVVREA DCGTTNYISV PLFQPDEVTR SLRLRKRADI EAGLYGRVLA
1160 1170 1180 1190 1200
REVEVLGVRL EEGRYLSMDD VHLLIKAAEA GEIQEVPVRS PLTCQTRYGV
1210 1220 1230 1240 1250
CQKCYGYDLS MARPVSIGEA VGIVAAQSIG EPGTQLTMRT FHTGGVAGAA
1260 1270 1280 1290 1300
DITQGLPRVI ELFEARRPKA KAVISEIDGV VRIEETEEKL SVFVESEGFS
1310 1320 1330 1340 1350
KEYKLPKEAR LLVKDGDYVE AGQPLTRGAI DPHQLLEAKG PEAVERYLVE
1360 1370 1380 1390 1400
EIQKVYRAQG VKLHDKHIEI VVRQMMKYVE VTDPGDSRLL EGQVLEKWDV
1410 1420 1430 1440 1450
EALNERLIAE GKTPVAWKPL LMGVTKSALS TKSWLSAASF QNTTHVLTEA
1460 1470 1480 1490 1500
AIAGKKDELI GLKENVILGR LIPAGTGSDF VRFTQVVDQK TLKAIEEARK
1510 1520
EAVEAKERPA ARRGVKREQP GKQA
Length:1,524
Mass (Da):170,756
Last modified:June 1, 2002 - v1
Checksum:iA7B335945B08E3A7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB083790 Genomic DNA. Translation: BAB89401.1.
AP008226 Genomic DNA. Translation: BAD71635.1.
RefSeqiWP_011228934.1. NC_006461.1.
YP_145078.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD71635; BAD71635; BAD71635.
GeneIDi3169754.
KEGGittj:TTHA1812.
PATRICi23958593. VBITheThe93045_1783.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB083790 Genomic DNA. Translation: BAB89401.1 .
AP008226 Genomic DNA. Translation: BAD71635.1 .
RefSeqi WP_011228934.1. NC_006461.1.
YP_145078.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1IW7 X-ray 2.60 D/N 1-1524 [» ]
1SMY X-ray 2.70 D/N 1-1524 [» ]
1ZYR X-ray 3.00 D/N 1-1524 [» ]
2A68 X-ray 2.50 D/N 1-1524 [» ]
2A69 X-ray 2.50 D/N 1-1524 [» ]
2A6E X-ray 2.80 D/N 1-1524 [» ]
2A6H X-ray 2.40 D/N 1-1524 [» ]
2BE5 X-ray 2.40 D/N 1-1524 [» ]
2CW0 X-ray 3.30 D/N 1-1524 [» ]
2O5I X-ray 2.50 D/N 1-1524 [» ]
2O5J X-ray 3.00 D/N 1-1524 [» ]
2PPB X-ray 3.00 D/N 1-1524 [» ]
3AOH X-ray 4.10 D/I/N 1-1524 [» ]
3AOI X-ray 4.30 D/I/N 1-1524 [» ]
3DXJ X-ray 3.00 D/N 1-1524 [» ]
3EQL X-ray 2.70 D/N 1-1524 [» ]
3WOD X-ray 3.60 D 1-1524 [» ]
4G7H X-ray 2.90 D/N 1-1524 [» ]
4G7O X-ray 2.99 D/N 1-1524 [» ]
4G7Z X-ray 3.82 D/N 1-1524 [» ]
4GZY X-ray 3.51 D 1-1524 [» ]
4GZZ X-ray 4.29 D 1-1524 [» ]
4MQ9 X-ray 3.35 D 1-1524 [» ]
4OIN X-ray 2.80 D 1-1524 [» ]
4OIO X-ray 3.10 D 1-1524 [» ]
4OIP X-ray 3.40 D 1-1524 [» ]
4OIQ X-ray 3.62 D 1-1524 [» ]
4OIR X-ray 3.10 D 1-1524 [» ]
4Q4Z X-ray 2.90 D 1-1524 [» ]
4Q5S X-ray 3.00 D 1-1524 [» ]
ProteinModelPortali Q8RQE8.
SMRi Q8RQE8. Positions 2-207.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-47007N.
IntActi Q8RQE8. 1 interaction.
STRINGi 300852.TTHA1812.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAD71635 ; BAD71635 ; BAD71635 .
GeneIDi 3169754.
KEGGi ttj:TTHA1812.
PATRICi 23958593. VBITheThe93045_1783.

Phylogenomic databases

eggNOGi COG0086.
HOGENOMi HOG000218387.
KOi K03046.
OMAi AYDSEFL.
OrthoDBi EOG6M9DS6.
PhylomeDBi Q8RQE8.

Enzyme and pathway databases

BioCyci TTHE300852:GH8R-1857-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q8RQE8.

Family and domain databases

HAMAPi MF_01322. RNApol_bact_RpoC.
InterProi IPR012754. DNA-dir_RpoC_beta_prime.
IPR000722. RNA_pol_asu.
IPR006592. RNA_pol_N.
IPR007080. RNA_pol_Rpb1_1.
IPR007066. RNA_pol_Rpb1_3.
IPR007083. RNA_pol_Rpb1_4.
IPR007081. RNA_pol_Rpb1_5.
[Graphical view ]
Pfami PF04997. RNA_pol_Rpb1_1. 2 hits.
PF00623. RNA_pol_Rpb1_2. 1 hit.
PF04983. RNA_pol_Rpb1_3. 1 hit.
PF05000. RNA_pol_Rpb1_4. 1 hit.
PF04998. RNA_pol_Rpb1_5. 1 hit.
[Graphical view ]
SMARTi SM00663. RPOLA_N. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Purification, crystallization and initial crystallographic analysis of RNA polymerase holoenzyme from Thermus thermophilus."
    Vassylyeva M.N., Lee J., Sekine S.I., Laptenko O., Kuramitsu S., Shibata T., Inoue Y., Borukhov S., Vassylyev D.G., Yokoyama S.
    Acta Crystallogr. D 58:1497-1500(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete genome sequence of Thermus thermophilus HB8."
    Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HB8 / ATCC 27634 / DSM 579.

Entry informationi

Entry nameiRPOC_THET8
AccessioniPrimary (citable) accession number: Q8RQE8
Secondary accession number(s): Q5SHB8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: June 1, 2002
Last modified: November 26, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3