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Q8RQE8 (RPOC_THET8) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA-directed RNA polymerase subunit beta'
Short name=RNAP subunit beta'
EC=2.7.7.6
Alternative name(s):
RNA polymerase subunit beta'
Transcriptase subunit beta'
Gene names
Name:rpoC
Ordered Locus Names:TTHA1812
OrganismThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifier300852 [NCBI]
Taxonomic lineageBacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus

Protein attributes

Sequence length1524 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. HAMAP MF_01322

Catalytic activity

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP MF_01322

Subunit structure

The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.

Sequence similarities

Belongs to the RNA polymerase beta' chain family.

Ontologies

Keywords
   Biological processTranscription
   Cellular componentDNA-directed RNA polymerase
   Molecular functionNucleotidyltransferase
Transferase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Molecular functionDNA binding

Inferred from electronic annotation. Source: InterPro

DNA-directed RNA polymerase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 15241524DNA-directed RNA polymerase subunit beta' HAMAP MF_01322
PRO_0000067820

Secondary structure

1 ................................................................................................................................................................................................................................. 1524
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q8RQE8 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: A7B335945B08E3A7

FASTA1,524170,756
        10         20         30         40         50         60 
MKKEVRKVRI ALASPEKIRS WSYGEVEKPE TINYRTLKPE RDGLFDERIF GPIKDYECAC 

        70         80         90        100        110        120 
GKYKRQRFEG KVCERCGVEV TKSIVRRYRM GHIELATPAA HIWFVKDVPS KIGTLLDLSA 

       130        140        150        160        170        180 
TELEQVLYFS KYIVLDPKGA ILNGVPVEKR QLLTDEEYRE LRYGKQETYP LPPGVDALVK 

       190        200        210        220        230        240 
DGEEVVKGQE LAPGVVSRLD GVALYRFPRR VRVEYVKKER AGLRLPLAAW VEKEAYKPGE 

       250        260        270        280        290        300 
ILAELPEPYL FRAEEEGVVE LKELEEGAFL VLRREDEPVA TYFLPVGMTP LVVHGEIVEK 

       310        320        330        340        350        360 
GQPLAEAKGL LRMPRQVRAA QVEAEEEGET VYLTLFLEWT EPKDYRVQPH MNVVVPEGAR 

       370        380        390        400        410        420 
VEAGDKIVAA IDPEEEVIAE AEGVVHLHEP ASILVVKARV YPFEDDVEVS TGDRVAPGDV 

       430        440        450        460        470        480 
LADGGKVKSD VYGRVEVDLV RNVVRVVESY DIDARMGAEA IQQLLKELDL EALEKELLEE 

       490        500        510        520        530        540 
MKHPSRARRA KARKRLEVVR AFLDSGNRPE WMILEAVPVL PPDLRPMVQV DGGRFATSDL 

       550        560        570        580        590        600 
NDLYRRLINR NNRLKKLLAQ GAPEIIIRNE KRMLQEAVDA LLDNGRRGAP VTNPGSDRPL 

       610        620        630        640        650        660 
RSLTDILSGK QGRFRQNLLG KRVDYSGRSV IVVGPQLKLH QCGLPKRMAL ELFKPFLLKK 

       670        680        690        700        710        720 
MEEKGIAPNV KAARRMLERQ RDIKDEVWDA LEEVIHGKVV LLNRAPTLHR LGIQAFQPVL 

       730        740        750        760        770        780 
VEGQSIQLHP LVCEAFNADF DGDQMAVHVP LSSFAQAEAR IQMLSAHNLL SPASGEPLAK 

       790        800        810        820        830        840 
PSRDIILGLY YITQVRKEKK GAGLEFATPE EALAAHERGE VALNAPIKVA GRETSVGRLK 

       850        860        870        880        890        900 
YVFANPDEAL LAVAHGIVDL QDVVTVRYMG KRLETSPGRI LFARIVAEAV EDEKVAWELI 

       910        920        930        940        950        960 
QLDVPQEKNS LKDLVYQAFL RLGMEKTARL LDALKYYGFT FSTTSGITIG IDDAVIPEEK 

       970        980        990       1000       1010       1020 
KQYLEEADRK LLQIEQAYEM GFLTDRERYD QILQLWTETT EKVTQAVFKN FEENYPFNPL 

      1030       1040       1050       1060       1070       1080 
YVMAQSGARG NPQQIRQLCG LRGLMQKPSG ETFEVPVRSS FREGLTVLEY FISSHGARKG 

      1090       1100       1110       1120       1130       1140 
GADTALRTAD SGYLTRKLVD VTHEIVVREA DCGTTNYISV PLFQPDEVTR SLRLRKRADI 

      1150       1160       1170       1180       1190       1200 
EAGLYGRVLA REVEVLGVRL EEGRYLSMDD VHLLIKAAEA GEIQEVPVRS PLTCQTRYGV 

      1210       1220       1230       1240       1250       1260 
CQKCYGYDLS MARPVSIGEA VGIVAAQSIG EPGTQLTMRT FHTGGVAGAA DITQGLPRVI 

      1270       1280       1290       1300       1310       1320 
ELFEARRPKA KAVISEIDGV VRIEETEEKL SVFVESEGFS KEYKLPKEAR LLVKDGDYVE 

      1330       1340       1350       1360       1370       1380 
AGQPLTRGAI DPHQLLEAKG PEAVERYLVE EIQKVYRAQG VKLHDKHIEI VVRQMMKYVE 

      1390       1400       1410       1420       1430       1440 
VTDPGDSRLL EGQVLEKWDV EALNERLIAE GKTPVAWKPL LMGVTKSALS TKSWLSAASF 

      1450       1460       1470       1480       1490       1500 
QNTTHVLTEA AIAGKKDELI GLKENVILGR LIPAGTGSDF VRFTQVVDQK TLKAIEEARK 

      1510       1520 
EAVEAKERPA ARRGVKREQP GKQA

« Hide

References

« Hide 'large scale' references
[1]"Purification, crystallization and initial crystallographic analysis of RNA polymerase holoenzyme from Thermus thermophilus."
Vassylyeva M.N., Lee J., Sekine S.I., Laptenko O., Kuramitsu S., Shibata T., Inoue Y., Borukhov S., Vassylyev D.G., Yokoyama S.
Acta Crystallogr. D 58:1497-1500(2002) [PubMed: 12198314] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete genome sequence of Thermus thermophilus HB8."
Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HB8 / ATCC 27634 / DSM 579.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AB083790 Genomic DNA. Translation: BAB89401.1.
AP008226 Genomic DNA. Translation: BAD71635.1.
RefSeqYP_145078.1. NC_006461.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1IW7X-ray2.60D/N1-1524[»]
1SMYX-ray2.70D/N1-1524[»]
1ZYRX-ray3.00D/N1-1524[»]
2A68X-ray2.50D/N1-1524[»]
2A69X-ray2.50D/N1-1524[»]
2A6EX-ray2.80D/N1-1524[»]
2A6HX-ray2.40D/N1-1524[»]
2BE5X-ray2.40D/N1-1524[»]
2CW0X-ray3.30D/N1-1524[»]
2O5IX-ray2.50D/N1-1524[»]
2O5JX-ray3.00D/N1-1524[»]
2PPBX-ray3.00D/N1-1524[»]
3AOHX-ray4.10D/I/N1-1524[»]
3AOIX-ray4.30D/I/N1-1524[»]
3DXJX-ray3.00D/N1-1524[»]
3EQLX-ray2.70D/N1-1524[»]
ProteinModelPortalQ8RQE8.
SMRQ8RQE8. Positions 2-207.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ8RQE8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3169754.
GenomeReviewsGene locus TTHA1812 in contig AP008226_GR.
KEGGttj:TTHA1812.
NMPDRfig|300852.3.peg.1221.
PATRIC23958593. VBITheThe93045_1783.

Phylogenomic databases

eggNOGCOG0086.
HOGENOMHBG621785.
OMADINDKHI.
PhylomeDBQ8RQE8.
ProtClustDBPRK00566.

Enzyme and pathway databases

BioCycTTHE300852:TTHA1812-MONOMER.

Family and domain databases

HAMAPMF_01322. RNApol_bact_RpoC.
[Tree]
InterProIPR012754. DNA-dir_RpoC_beta_prime.
IPR000722. RNA_pol_asu.
IPR006592. RNA_pol_N.
IPR007080. RNA_pol_Rpb1_1.
IPR007066. RNA_pol_Rpb1_3.
IPR007083. RNA_pol_Rpb1_4.
IPR007081. RNA_pol_Rpb1_5.
[Graphical view]
KOK03046.
PfamPF04997. RNA_pol_Rpb1_1. 2 hits.
PF00623. RNA_pol_Rpb1_2. 1 hit.
PF04983. RNA_pol_Rpb1_3. 1 hit.
PF05000. RNA_pol_Rpb1_4. 1 hit.
PF04998. RNA_pol_Rpb1_5. 1 hit.
[Graphical view]
SMARTSM00663. RPOLA_N. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRPOC_THET8
AccessionPrimary (citable) accession number: Q8RQE8
Secondary accession number(s): Q5SHB8
Entry history
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: June 1, 2002
Last modified: January 25, 2012
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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