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Protein

DNA-directed RNA polymerase subunit beta'

Gene

rpoC

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Transcription

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase subunit beta'UniRule annotation (EC:2.7.7.6UniRule annotation)
Short name:
RNAP subunit beta'UniRule annotation
Alternative name(s):
RNA polymerase subunit beta'UniRule annotation
Transcriptase subunit beta'UniRule annotation
Gene namesi
Name:rpoCUniRule annotation
Ordered Locus Names:TTHA1812
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Subcellular locationi

Keywords - Cellular componenti

DNA-directed RNA polymerase

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000678201 – 1524DNA-directed RNA polymerase subunit beta'Add BLAST1524

Interactioni

Subunit structurei

The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.

Protein-protein interaction databases

DIPiDIP-47007N.
IntActiQ8RQE8. 1 interactor.
STRINGi300852.TTHA1812.

Structurei

Secondary structure

11524
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 11Combined sources5
Helixi15 – 21Combined sources7
Beta strandi22 – 25Combined sources4
Beta strandi36 – 39Combined sources4
Beta strandi41 – 43Combined sources3
Turni47 – 50Combined sources4
Beta strandi53 – 56Combined sources4
Beta strandi59 – 63Combined sources5
Turni67 – 70Combined sources4
Beta strandi74 – 76Combined sources3
Beta strandi81 – 83Combined sources3
Helixi84 – 88Combined sources5
Beta strandi90 – 100Combined sources11
Helixi102 – 105Combined sources4
Beta strandi106 – 109Combined sources4
Helixi111 – 115Combined sources5
Helixi122 – 129Combined sources8
Beta strandi133 – 135Combined sources3
Beta strandi143 – 146Combined sources4
Helixi155 – 162Combined sources8
Beta strandi167 – 170Combined sources4
Beta strandi173 – 176Combined sources4
Helixi177 – 186Combined sources10
Beta strandi187 – 189Combined sources3
Beta strandi191 – 194Combined sources4
Beta strandi195 – 197Combined sources3
Beta strandi199 – 201Combined sources3
Beta strandi204 – 206Combined sources3
Beta strandi207 – 209Combined sources3
Beta strandi211 – 213Combined sources3
Beta strandi217 – 222Combined sources6
Turni226 – 231Combined sources6
Helixi233 – 236Combined sources4
Beta strandi237 – 239Combined sources3
Helixi241 – 244Combined sources4
Beta strandi245 – 247Combined sources3
Helixi248 – 250Combined sources3
Beta strandi257 – 263Combined sources7
Beta strandi268 – 284Combined sources17
Beta strandi286 – 290Combined sources5
Beta strandi297 – 300Combined sources4
Beta strandi303 – 312Combined sources10
Beta strandi315 – 327Combined sources13
Beta strandi330 – 346Combined sources17
Beta strandi359 – 361Combined sources3
Beta strandi366 – 369Combined sources4
Helixi373 – 375Combined sources3
Turni378 – 381Combined sources4
Beta strandi383 – 387Combined sources5
Beta strandi391 – 394Combined sources4
Beta strandi396 – 401Combined sources6
Beta strandi407 – 409Combined sources3
Beta strandi415 – 417Combined sources3
Beta strandi420 – 422Combined sources3
Beta strandi423 – 425Combined sources3
Beta strandi426 – 428Combined sources3
Beta strandi434 – 438Combined sources5
Turni439 – 442Combined sources4
Beta strandi443 – 447Combined sources5
Beta strandi452 – 455Combined sources4
Helixi458 – 467Combined sources10
Helixi470 – 482Combined sources13
Helixi486 – 504Combined sources19
Turni509 – 512Combined sources4
Beta strandi513 – 519Combined sources7
Turni522 – 524Combined sources3
Beta strandi527 – 529Combined sources3
Beta strandi531 – 533Combined sources3
Beta strandi535 – 537Combined sources3
Helixi540 – 560Combined sources21
Helixi564 – 582Combined sources19
Beta strandi584 – 588Combined sources5
Beta strandi593 – 595Combined sources3
Beta strandi596 – 599Combined sources4
Helixi603 – 607Combined sources5
Turni609 – 611Combined sources3
Helixi613 – 619Combined sources7
Beta strandi621 – 633Combined sources13
Beta strandi635 – 637Combined sources3
Beta strandi641 – 645Combined sources5
Helixi646 – 652Combined sources7
Helixi654 – 663Combined sources10
Beta strandi664 – 666Combined sources3
Beta strandi667 – 669Combined sources3
Helixi670 – 677Combined sources8
Helixi680 – 682Combined sources3
Helixi685 – 694Combined sources10
Turni695 – 697Combined sources3
Beta strandi700 – 703Combined sources4
Helixi710 – 712Combined sources3
Beta strandi713 – 724Combined sources12
Beta strandi726 – 728Combined sources3
Helixi731 – 733Combined sources3
Turni734 – 737Combined sources4
Turni740 – 742Combined sources3
Beta strandi744 – 748Combined sources5
Helixi753 – 762Combined sources10
Turni765 – 767Combined sources3
Beta strandi772 – 779Combined sources8
Helixi783 – 793Combined sources11
Beta strandi799 – 801Combined sources3
Beta strandi805 – 808Combined sources4
Helixi810 – 821Combined sources12
Beta strandi823 – 825Combined sources3
Beta strandi826 – 829Combined sources4
Beta strandi832 – 835Combined sources4
Helixi836 – 840Combined sources5
Beta strandi842 – 845Combined sources4
Helixi846 – 854Combined sources9
Beta strandi860 – 862Combined sources3
Beta strandi865 – 867Combined sources3
Beta strandi871 – 873Combined sources3
Helixi877 – 889Combined sources13
Helixi893 – 898Combined sources6
Beta strandi902 – 904Combined sources3
Helixi908 – 921Combined sources14
Helixi924 – 945Combined sources22
Turni951 – 953Combined sources3
Helixi960 – 979Combined sources20
Beta strandi980 – 983Combined sources4
Helixi985 – 1013Combined sources29
Helixi1019 – 1025Combined sources7
Beta strandi1026 – 1029Combined sources4
Helixi1032 – 1038Combined sources7
Beta strandi1048 – 1050Combined sources3
Beta strandi1061 – 1063Combined sources3
Helixi1068 – 1102Combined sources35
Beta strandi1106 – 1110Combined sources5
Beta strandi1118 – 1123Combined sources6
Beta strandi1127 – 1130Combined sources4
Beta strandi1131 – 1134Combined sources4
Helixi1137 – 1144Combined sources8
Beta strandi1148 – 1151Combined sources4
Beta strandi1153 – 1155Combined sources3
Beta strandi1156 – 1160Combined sources5
Turni1168 – 1172Combined sources5
Helixi1173 – 1180Combined sources8
Beta strandi1184 – 1188Combined sources5
Helixi1191 – 1193Combined sources3
Beta strandi1197 – 1200Combined sources4
Helixi1202 – 1205Combined sources4
Beta strandi1209 – 1214Combined sources6
Helixi1221 – 1230Combined sources10
Helixi1232 – 1234Combined sources3
Helixi1240 – 1243Combined sources4
Beta strandi1246 – 1248Combined sources3
Helixi1256 – 1263Combined sources8
Beta strandi1269 – 1271Combined sources3
Beta strandi1279 – 1283Combined sources5
Beta strandi1290 – 1295Combined sources6
Beta strandi1300 – 1305Combined sources6
Beta strandi1317 – 1319Combined sources3
Beta strandi1324 – 1329Combined sources6
Helixi1332 – 1339Combined sources8
Helixi1341 – 1357Combined sources17
Turni1358 – 1360Combined sources3
Helixi1365 – 1374Combined sources10
Beta strandi1377 – 1383Combined sources7
Beta strandi1385 – 1387Combined sources3
Beta strandi1392 – 1394Combined sources3
Helixi1395 – 1406Combined sources12
Turni1409 – 1411Combined sources3
Beta strandi1416 – 1419Combined sources4
Helixi1424 – 1429Combined sources6
Helixi1434 – 1439Combined sources6
Helixi1443 – 1452Combined sources10
Beta strandi1455 – 1458Combined sources4
Helixi1462 – 1467Combined sources6
Beta strandi1473 – 1475Combined sources3
Turni1479 – 1481Combined sources3
Beta strandi1485 – 1487Combined sources3
Helixi1489 – 1500Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IW7X-ray2.60D/N1-1524[»]
1SMYX-ray2.70D/N1-1524[»]
1ZYRX-ray3.00D/N1-1524[»]
2A68X-ray2.50D/N1-1524[»]
2A69X-ray2.50D/N1-1524[»]
2A6EX-ray2.80D/N1-1524[»]
2A6HX-ray2.40D/N1-1524[»]
2BE5X-ray2.40D/N1-1524[»]
2CW0X-ray3.30D/N1-1524[»]
2O5IX-ray2.50D/N1-1524[»]
2O5JX-ray3.00D/N1-1524[»]
2PPBX-ray3.00D/N1-1524[»]
3AOHX-ray4.10D/I/N1-1524[»]
3AOIX-ray4.30D/I/N1-1524[»]
3DXJX-ray3.00D/N1-1524[»]
3EQLX-ray2.70D/N1-1524[»]
3WODX-ray3.60D1-1524[»]
4G7HX-ray2.90D/N1-1524[»]
4G7OX-ray2.99D/N1-1524[»]
4G7ZX-ray3.82D/N1-1524[»]
4GZYX-ray3.51D1-1524[»]
4GZZX-ray4.29D1-1524[»]
4MQ9X-ray3.35D1-1524[»]
4OINX-ray2.80D1-1524[»]
4OIOX-ray3.10D1-1524[»]
4OIPX-ray3.40D1-1524[»]
4OIQX-ray3.62D1-1524[»]
4OIRX-ray3.10D1-1524[»]
4Q4ZX-ray2.90D1-1524[»]
4Q5SX-ray3.00D1-1524[»]
4WQSX-ray4.31D/N1-1524[»]
4WQTX-ray4.40D/I/N1-1524[»]
5D4CX-ray3.28D/N1-1524[»]
5D4DX-ray3.00D/N1-1524[»]
5D4EX-ray3.08D/N1-1524[»]
5E17X-ray3.20D1-1524[»]
5E18X-ray3.30D1-1524[»]
5I2DX-ray4.41D/O1-1524[»]
ProteinModelPortaliQ8RQE8.
SMRiQ8RQE8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8RQE8.

Family & Domainsi

Sequence similaritiesi

Belongs to the RNA polymerase beta' chain family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D27. Bacteria.
COG0086. LUCA.
HOGENOMiHOG000218387.
KOiK03046.
OMAiYFAAYMI.
PhylomeDBiQ8RQE8.

Family and domain databases

HAMAPiMF_01322. RNApol_bact_RpoC. 1 hit.
InterProiIPR012754. DNA-dir_RpoC_beta_prime.
IPR000722. RNA_pol_asu.
IPR006592. RNA_pol_N.
IPR007080. RNA_pol_Rpb1_1.
IPR007066. RNA_pol_Rpb1_3.
IPR007083. RNA_pol_Rpb1_4.
IPR007081. RNA_pol_Rpb1_5.
[Graphical view]
PfamiPF04997. RNA_pol_Rpb1_1. 2 hits.
PF00623. RNA_pol_Rpb1_2. 1 hit.
PF04983. RNA_pol_Rpb1_3. 1 hit.
PF05000. RNA_pol_Rpb1_4. 1 hit.
PF04998. RNA_pol_Rpb1_5. 2 hits.
[Graphical view]
SMARTiSM00663. RPOLA_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8RQE8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKEVRKVRI ALASPEKIRS WSYGEVEKPE TINYRTLKPE RDGLFDERIF
60 70 80 90 100
GPIKDYECAC GKYKRQRFEG KVCERCGVEV TKSIVRRYRM GHIELATPAA
110 120 130 140 150
HIWFVKDVPS KIGTLLDLSA TELEQVLYFS KYIVLDPKGA ILNGVPVEKR
160 170 180 190 200
QLLTDEEYRE LRYGKQETYP LPPGVDALVK DGEEVVKGQE LAPGVVSRLD
210 220 230 240 250
GVALYRFPRR VRVEYVKKER AGLRLPLAAW VEKEAYKPGE ILAELPEPYL
260 270 280 290 300
FRAEEEGVVE LKELEEGAFL VLRREDEPVA TYFLPVGMTP LVVHGEIVEK
310 320 330 340 350
GQPLAEAKGL LRMPRQVRAA QVEAEEEGET VYLTLFLEWT EPKDYRVQPH
360 370 380 390 400
MNVVVPEGAR VEAGDKIVAA IDPEEEVIAE AEGVVHLHEP ASILVVKARV
410 420 430 440 450
YPFEDDVEVS TGDRVAPGDV LADGGKVKSD VYGRVEVDLV RNVVRVVESY
460 470 480 490 500
DIDARMGAEA IQQLLKELDL EALEKELLEE MKHPSRARRA KARKRLEVVR
510 520 530 540 550
AFLDSGNRPE WMILEAVPVL PPDLRPMVQV DGGRFATSDL NDLYRRLINR
560 570 580 590 600
NNRLKKLLAQ GAPEIIIRNE KRMLQEAVDA LLDNGRRGAP VTNPGSDRPL
610 620 630 640 650
RSLTDILSGK QGRFRQNLLG KRVDYSGRSV IVVGPQLKLH QCGLPKRMAL
660 670 680 690 700
ELFKPFLLKK MEEKGIAPNV KAARRMLERQ RDIKDEVWDA LEEVIHGKVV
710 720 730 740 750
LLNRAPTLHR LGIQAFQPVL VEGQSIQLHP LVCEAFNADF DGDQMAVHVP
760 770 780 790 800
LSSFAQAEAR IQMLSAHNLL SPASGEPLAK PSRDIILGLY YITQVRKEKK
810 820 830 840 850
GAGLEFATPE EALAAHERGE VALNAPIKVA GRETSVGRLK YVFANPDEAL
860 870 880 890 900
LAVAHGIVDL QDVVTVRYMG KRLETSPGRI LFARIVAEAV EDEKVAWELI
910 920 930 940 950
QLDVPQEKNS LKDLVYQAFL RLGMEKTARL LDALKYYGFT FSTTSGITIG
960 970 980 990 1000
IDDAVIPEEK KQYLEEADRK LLQIEQAYEM GFLTDRERYD QILQLWTETT
1010 1020 1030 1040 1050
EKVTQAVFKN FEENYPFNPL YVMAQSGARG NPQQIRQLCG LRGLMQKPSG
1060 1070 1080 1090 1100
ETFEVPVRSS FREGLTVLEY FISSHGARKG GADTALRTAD SGYLTRKLVD
1110 1120 1130 1140 1150
VTHEIVVREA DCGTTNYISV PLFQPDEVTR SLRLRKRADI EAGLYGRVLA
1160 1170 1180 1190 1200
REVEVLGVRL EEGRYLSMDD VHLLIKAAEA GEIQEVPVRS PLTCQTRYGV
1210 1220 1230 1240 1250
CQKCYGYDLS MARPVSIGEA VGIVAAQSIG EPGTQLTMRT FHTGGVAGAA
1260 1270 1280 1290 1300
DITQGLPRVI ELFEARRPKA KAVISEIDGV VRIEETEEKL SVFVESEGFS
1310 1320 1330 1340 1350
KEYKLPKEAR LLVKDGDYVE AGQPLTRGAI DPHQLLEAKG PEAVERYLVE
1360 1370 1380 1390 1400
EIQKVYRAQG VKLHDKHIEI VVRQMMKYVE VTDPGDSRLL EGQVLEKWDV
1410 1420 1430 1440 1450
EALNERLIAE GKTPVAWKPL LMGVTKSALS TKSWLSAASF QNTTHVLTEA
1460 1470 1480 1490 1500
AIAGKKDELI GLKENVILGR LIPAGTGSDF VRFTQVVDQK TLKAIEEARK
1510 1520
EAVEAKERPA ARRGVKREQP GKQA
Length:1,524
Mass (Da):170,756
Last modified:June 1, 2002 - v1
Checksum:iA7B335945B08E3A7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB083790 Genomic DNA. Translation: BAB89401.1.
AP008226 Genomic DNA. Translation: BAD71635.1.
RefSeqiWP_011228934.1. NC_006461.1.
YP_145078.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD71635; BAD71635; BAD71635.
GeneIDi3169754.
KEGGittj:TTHA1812.
PATRICi23958593. VBITheThe93045_1783.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB083790 Genomic DNA. Translation: BAB89401.1.
AP008226 Genomic DNA. Translation: BAD71635.1.
RefSeqiWP_011228934.1. NC_006461.1.
YP_145078.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IW7X-ray2.60D/N1-1524[»]
1SMYX-ray2.70D/N1-1524[»]
1ZYRX-ray3.00D/N1-1524[»]
2A68X-ray2.50D/N1-1524[»]
2A69X-ray2.50D/N1-1524[»]
2A6EX-ray2.80D/N1-1524[»]
2A6HX-ray2.40D/N1-1524[»]
2BE5X-ray2.40D/N1-1524[»]
2CW0X-ray3.30D/N1-1524[»]
2O5IX-ray2.50D/N1-1524[»]
2O5JX-ray3.00D/N1-1524[»]
2PPBX-ray3.00D/N1-1524[»]
3AOHX-ray4.10D/I/N1-1524[»]
3AOIX-ray4.30D/I/N1-1524[»]
3DXJX-ray3.00D/N1-1524[»]
3EQLX-ray2.70D/N1-1524[»]
3WODX-ray3.60D1-1524[»]
4G7HX-ray2.90D/N1-1524[»]
4G7OX-ray2.99D/N1-1524[»]
4G7ZX-ray3.82D/N1-1524[»]
4GZYX-ray3.51D1-1524[»]
4GZZX-ray4.29D1-1524[»]
4MQ9X-ray3.35D1-1524[»]
4OINX-ray2.80D1-1524[»]
4OIOX-ray3.10D1-1524[»]
4OIPX-ray3.40D1-1524[»]
4OIQX-ray3.62D1-1524[»]
4OIRX-ray3.10D1-1524[»]
4Q4ZX-ray2.90D1-1524[»]
4Q5SX-ray3.00D1-1524[»]
4WQSX-ray4.31D/N1-1524[»]
4WQTX-ray4.40D/I/N1-1524[»]
5D4CX-ray3.28D/N1-1524[»]
5D4DX-ray3.00D/N1-1524[»]
5D4EX-ray3.08D/N1-1524[»]
5E17X-ray3.20D1-1524[»]
5E18X-ray3.30D1-1524[»]
5I2DX-ray4.41D/O1-1524[»]
ProteinModelPortaliQ8RQE8.
SMRiQ8RQE8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-47007N.
IntActiQ8RQE8. 1 interactor.
STRINGi300852.TTHA1812.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD71635; BAD71635; BAD71635.
GeneIDi3169754.
KEGGittj:TTHA1812.
PATRICi23958593. VBITheThe93045_1783.

Phylogenomic databases

eggNOGiENOG4105D27. Bacteria.
COG0086. LUCA.
HOGENOMiHOG000218387.
KOiK03046.
OMAiYFAAYMI.
PhylomeDBiQ8RQE8.

Miscellaneous databases

EvolutionaryTraceiQ8RQE8.
PROiQ8RQE8.

Family and domain databases

HAMAPiMF_01322. RNApol_bact_RpoC. 1 hit.
InterProiIPR012754. DNA-dir_RpoC_beta_prime.
IPR000722. RNA_pol_asu.
IPR006592. RNA_pol_N.
IPR007080. RNA_pol_Rpb1_1.
IPR007066. RNA_pol_Rpb1_3.
IPR007083. RNA_pol_Rpb1_4.
IPR007081. RNA_pol_Rpb1_5.
[Graphical view]
PfamiPF04997. RNA_pol_Rpb1_1. 2 hits.
PF00623. RNA_pol_Rpb1_2. 1 hit.
PF04983. RNA_pol_Rpb1_3. 1 hit.
PF05000. RNA_pol_Rpb1_4. 1 hit.
PF04998. RNA_pol_Rpb1_5. 2 hits.
[Graphical view]
SMARTiSM00663. RPOLA_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRPOC_THET8
AccessioniPrimary (citable) accession number: Q8RQE8
Secondary accession number(s): Q5SHB8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: June 1, 2002
Last modified: November 2, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.