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Q8RQE7 (RPOZ_THET8) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA-directed RNA polymerase subunit omega
Short name=RNAP omega subunit
EC=2.7.7.6
Alternative name(s):
RNA polymerase omega subunit
Transcriptase subunit omega
Gene names
Name:rpoZ
Ordered Locus Names:TTHA1561
OrganismThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Reference proteome] [HAMAP]
Taxonomic identifier300852 [NCBI]
Taxonomic lineageBacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus ›

Protein attributes

Sequence length99 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Promotes RNA polymerase assembly. Latches the N- and C-terminal regions of the beta' subunit thereby facilitating its interaction with the beta and alpha subunits By similarity. HAMAP-Rule MF_00366

Catalytic activity

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP-Rule MF_00366

Subunit structure

The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.

Sequence similarities

Belongs to the RNA polymerase subunit omega family.

Ontologies

Keywords
   Biological processTranscription
   Cellular componentDNA-directed RNA polymerase
   Molecular functionNucleotidyltransferase
Transferase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtranscription, DNA-dependent

Inferred from electronic annotation. Source: HAMAP

   Cellular_componentRNA polymerase complex

Inferred from electronic annotation. Source: InterPro

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: HAMAP

DNA-directed RNA polymerase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 9999DNA-directed RNA polymerase subunit omega HAMAP-Rule MF_00366
PRO_0000129004

Experimental info

Sequence conflict611V → E in BAB89402. Ref.1
Sequence conflict921L → I in BAB89402. Ref.1
Sequence conflict951V → G in BAB89402. Ref.1

Secondary structure

1 .................... 99
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q8RQE7 [UniParc].

Last modified March 29, 2005. Version 2.
Checksum: 1D213933B0B98965

FASTA9911,517
        10         20         30         40         50         60 
MAEPGIDKLF GMVDSKYRLT VVVAKRAQQL LRHGFKNTVL EPEERPKMQT LEGLFDDPNA 

        70         80         90 
VTWAMKELLT GRLVFGENLV PEDRLQKEME RLYPVEREE

« Hide

References

« Hide 'large scale' references
[1]"Purification, crystallization and initial crystallographic analysis of RNA polymerase holoenzyme from Thermus thermophilus."
Vassylyeva M.N., Lee J., Sekine S.I., Laptenko O., Kuramitsu S., Shibata T., Inoue Y., Borukhov S., Vassylyev D.G., Yokoyama S.
Acta Crystallogr. D 58:1497-1500(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete genome sequence of Thermus thermophilus HB8."
Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HB8 / ATCC 27634 / DSM 579.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AB083791 Genomic DNA. Translation: BAB89402.1.
AP008226 Genomic DNA. Translation: BAD71384.1.
RefSeqYP_144827.1. NC_006461.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1IW7X-ray2.60E/O1-99[»]
1SMYX-ray2.70E/O1-99[»]
1ZYRX-ray3.00E/O1-99[»]
2A68X-ray2.50E/O1-99[»]
2A69X-ray2.50E/O1-99[»]
2A6EX-ray2.80E/O1-99[»]
2A6HX-ray2.40E/O1-99[»]
2BE5X-ray2.40E/O1-99[»]
2CW0X-ray3.30E/O1-99[»]
2O5IX-ray2.50E/O1-99[»]
2O5JX-ray3.00E/O1-99[»]
2PPBX-ray3.00E/O1-99[»]
3AOHX-ray4.10E/J/O1-99[»]
3AOIX-ray4.30E/J/O1-99[»]
3DXJX-ray3.00E/O1-99[»]
3EQLX-ray2.70E/O1-99[»]
4G7HX-ray2.90E/O1-99[»]
4G7OX-ray2.99E/O1-99[»]
4G7ZX-ray3.82E/O1-99[»]
4GZYX-ray3.51E1-99[»]
4GZZX-ray4.29E1-99[»]
ProteinModelPortalQ8RQE7.
SMRQ8RQE7. Positions 2-96.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-47008N.
STRING300852.TTHA1561.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD71384; BAD71384; BAD71384.
GeneID3169896.
KEGGttj:TTHA1561.
PATRIC23958079. VBITheThe93045_1532.

Phylogenomic databases

eggNOGCOG1758.
HOGENOMHOG000099539.
KOK03060.
OMADSKYRLS.
ProtClustDBPRK00392.

Family and domain databases

Gene3D3.90.940.10. 1 hit.
HAMAPMF_00366. RNApol_bact_RpoZ.
InterProIPR003716. DNA-dir_RNA_pol_omega.
IPR006110. RNA_poly_omega/K/RPABC2.
IPR012293. RNAP_RPB6_omega.
[Graphical view]
PfamPF01192. RNA_pol_Rpb6. 1 hit.
[Graphical view]
SUPFAMSSF63562. RNA_pol_omega. 1 hit.
TIGRFAMsTIGR00690. rpoZ. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceQ8RQE7.

Entry information

Entry nameRPOZ_THET8
AccessionPrimary (citable) accession number: Q8RQE7
Secondary accession number(s): Q5SI19
Entry history
Integrated into UniProtKB/Swiss-Prot: February 12, 2003
Last sequence update: March 29, 2005
Last modified: May 1, 2013
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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