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Q8RQE7

- RPOZ_THET8

UniProt

Q8RQE7 - RPOZ_THET8

Protein

DNA-directed RNA polymerase subunit omega

Gene

rpoZ

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Promotes RNA polymerase assembly. Latches the N- and C-terminal regions of the beta' subunit thereby facilitating its interaction with the beta and alpha subunits.UniRule annotation

    Catalytic activityi

    Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).UniRule annotation

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-HAMAP
    2. DNA-directed RNA polymerase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. transcription, DNA-templated Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    Transcription

    Enzyme and pathway databases

    BioCyciTTHE300852:GH8R-1600-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA-directed RNA polymerase subunit omegaUniRule annotation (EC:2.7.7.6UniRule annotation)
    Short name:
    RNAP omega subunitUniRule annotation
    Alternative name(s):
    RNA polymerase omega subunitUniRule annotation
    Transcriptase subunit omegaUniRule annotation
    Gene namesi
    Name:rpoZUniRule annotation
    Ordered Locus Names:TTHA1561
    OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
    Taxonomic identifieri300852 [NCBI]
    Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
    ProteomesiUP000000532: Chromosome

    Subcellular locationi

    Keywords - Cellular componenti

    DNA-directed RNA polymerase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 9999DNA-directed RNA polymerase subunit omegaPRO_0000129004Add
    BLAST

    Interactioni

    Subunit structurei

    The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.UniRule annotation

    Protein-protein interaction databases

    DIPiDIP-47008N.
    IntActiQ8RQE7. 1 interaction.
    STRINGi300852.TTHA1561.

    Structurei

    Secondary structure

    1
    99
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi6 – 127
    Beta strandi13 – 153
    Helixi16 – 3217
    Helixi35 – 373
    Beta strandi42 – 443
    Beta strandi49 – 524
    Helixi53 – 553
    Beta strandi56 – 583
    Helixi61 – 688
    Turni69 – 713
    Beta strandi74 – 807
    Helixi84 – 929

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1IW7X-ray2.60E/O1-99[»]
    1SMYX-ray2.70E/O1-99[»]
    1ZYRX-ray3.00E/O1-99[»]
    2A68X-ray2.50E/O1-99[»]
    2A69X-ray2.50E/O1-99[»]
    2A6EX-ray2.80E/O1-99[»]
    2A6HX-ray2.40E/O1-99[»]
    2BE5X-ray2.40E/O1-99[»]
    2CW0X-ray3.30E/O1-99[»]
    2O5IX-ray2.50E/O1-99[»]
    2O5JX-ray3.00E/O1-99[»]
    2PPBX-ray3.00E/O1-99[»]
    3AOHX-ray4.10E/J/O1-99[»]
    3AOIX-ray4.30E/J/O1-99[»]
    3DXJX-ray3.00E/O1-99[»]
    3EQLX-ray2.70E/O1-99[»]
    3WODX-ray3.60E1-99[»]
    4G7HX-ray2.90E/O1-99[»]
    4G7OX-ray2.99E/O1-99[»]
    4G7ZX-ray3.82E/O1-99[»]
    4GZYX-ray3.51E1-99[»]
    4GZZX-ray4.29E1-99[»]
    4MQ9X-ray3.35E1-99[»]
    4OINX-ray2.80E1-99[»]
    4OIOX-ray3.10E1-99[»]
    4OIPX-ray3.40E1-99[»]
    4OIQX-ray3.62E1-99[»]
    4OIRX-ray3.10E1-99[»]
    ProteinModelPortaliQ8RQE7.
    SMRiQ8RQE7. Positions 2-96.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ8RQE7.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the RNA polymerase subunit omega family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1758.
    HOGENOMiHOG000099539.
    KOiK03060.
    OMAiDSKYRLS.
    OrthoDBiEOG6Q5NV5.

    Family and domain databases

    Gene3Di3.90.940.10. 1 hit.
    HAMAPiMF_00366. RNApol_bact_RpoZ.
    InterProiIPR003716. DNA-dir_RNA_pol_omega.
    IPR006110. Pol_omega/K/RPB6.
    IPR012293. RNAP_RPB6_omega.
    [Graphical view]
    PfamiPF01192. RNA_pol_Rpb6. 1 hit.
    [Graphical view]
    SUPFAMiSSF63562. SSF63562. 1 hit.
    TIGRFAMsiTIGR00690. rpoZ. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8RQE7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAEPGIDKLF GMVDSKYRLT VVVAKRAQQL LRHGFKNTVL EPEERPKMQT   50
    LEGLFDDPNA VTWAMKELLT GRLVFGENLV PEDRLQKEME RLYPVEREE 99
    Length:99
    Mass (Da):11,517
    Last modified:March 29, 2005 - v2
    Checksum:i1D213933B0B98965
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti61 – 611V → E in BAB89402. (PubMed:12198314)Curated
    Sequence conflicti92 – 921L → I in BAB89402. (PubMed:12198314)Curated
    Sequence conflicti95 – 951V → G in BAB89402. (PubMed:12198314)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB083791 Genomic DNA. Translation: BAB89402.1.
    AP008226 Genomic DNA. Translation: BAD71384.1.
    RefSeqiYP_144827.1. NC_006461.1.

    Genome annotation databases

    EnsemblBacteriaiBAD71384; BAD71384; BAD71384.
    GeneIDi3169896.
    KEGGittj:TTHA1561.
    PATRICi23958079. VBITheThe93045_1532.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB083791 Genomic DNA. Translation: BAB89402.1 .
    AP008226 Genomic DNA. Translation: BAD71384.1 .
    RefSeqi YP_144827.1. NC_006461.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1IW7 X-ray 2.60 E/O 1-99 [» ]
    1SMY X-ray 2.70 E/O 1-99 [» ]
    1ZYR X-ray 3.00 E/O 1-99 [» ]
    2A68 X-ray 2.50 E/O 1-99 [» ]
    2A69 X-ray 2.50 E/O 1-99 [» ]
    2A6E X-ray 2.80 E/O 1-99 [» ]
    2A6H X-ray 2.40 E/O 1-99 [» ]
    2BE5 X-ray 2.40 E/O 1-99 [» ]
    2CW0 X-ray 3.30 E/O 1-99 [» ]
    2O5I X-ray 2.50 E/O 1-99 [» ]
    2O5J X-ray 3.00 E/O 1-99 [» ]
    2PPB X-ray 3.00 E/O 1-99 [» ]
    3AOH X-ray 4.10 E/J/O 1-99 [» ]
    3AOI X-ray 4.30 E/J/O 1-99 [» ]
    3DXJ X-ray 3.00 E/O 1-99 [» ]
    3EQL X-ray 2.70 E/O 1-99 [» ]
    3WOD X-ray 3.60 E 1-99 [» ]
    4G7H X-ray 2.90 E/O 1-99 [» ]
    4G7O X-ray 2.99 E/O 1-99 [» ]
    4G7Z X-ray 3.82 E/O 1-99 [» ]
    4GZY X-ray 3.51 E 1-99 [» ]
    4GZZ X-ray 4.29 E 1-99 [» ]
    4MQ9 X-ray 3.35 E 1-99 [» ]
    4OIN X-ray 2.80 E 1-99 [» ]
    4OIO X-ray 3.10 E 1-99 [» ]
    4OIP X-ray 3.40 E 1-99 [» ]
    4OIQ X-ray 3.62 E 1-99 [» ]
    4OIR X-ray 3.10 E 1-99 [» ]
    ProteinModelPortali Q8RQE7.
    SMRi Q8RQE7. Positions 2-96.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-47008N.
    IntActi Q8RQE7. 1 interaction.
    STRINGi 300852.TTHA1561.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAD71384 ; BAD71384 ; BAD71384 .
    GeneIDi 3169896.
    KEGGi ttj:TTHA1561.
    PATRICi 23958079. VBITheThe93045_1532.

    Phylogenomic databases

    eggNOGi COG1758.
    HOGENOMi HOG000099539.
    KOi K03060.
    OMAi DSKYRLS.
    OrthoDBi EOG6Q5NV5.

    Enzyme and pathway databases

    BioCyci TTHE300852:GH8R-1600-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q8RQE7.

    Family and domain databases

    Gene3Di 3.90.940.10. 1 hit.
    HAMAPi MF_00366. RNApol_bact_RpoZ.
    InterProi IPR003716. DNA-dir_RNA_pol_omega.
    IPR006110. Pol_omega/K/RPB6.
    IPR012293. RNAP_RPB6_omega.
    [Graphical view ]
    Pfami PF01192. RNA_pol_Rpb6. 1 hit.
    [Graphical view ]
    SUPFAMi SSF63562. SSF63562. 1 hit.
    TIGRFAMsi TIGR00690. rpoZ. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Purification, crystallization and initial crystallographic analysis of RNA polymerase holoenzyme from Thermus thermophilus."
      Vassylyeva M.N., Lee J., Sekine S.I., Laptenko O., Kuramitsu S., Shibata T., Inoue Y., Borukhov S., Vassylyev D.G., Yokoyama S.
      Acta Crystallogr. D 58:1497-1500(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Complete genome sequence of Thermus thermophilus HB8."
      Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
      Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: HB8 / ATCC 27634 / DSM 579.

    Entry informationi

    Entry nameiRPOZ_THET8
    AccessioniPrimary (citable) accession number: Q8RQE7
    Secondary accession number(s): Q5SI19
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 12, 2003
    Last sequence update: March 29, 2005
    Last modified: October 1, 2014
    This is version 88 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3