Reviewed, UniProtKB/Swiss-Prot Q8RQD1 (GLND_AZOBR)

Names and origin

Protein names

Recommended name:
    [Protein-PII] uridylyltransferase
      Short name=PII uridylyl-transferase
    EC=2.7.7.59
Alternative name(s):
    Uridylyl-removing enzyme
    UTase

Gene names

Name:

glnD

Organism

Azospirillum brasilense

Taxonomic identifier

192 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Rhodospirillaceae › Azospirillum

Protein attributes

Sequence length	933 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Modifies, by uridylylation or deuridylylation the PII (glnB) regulatory protein. HAMAP MF_00277
Catalytic activity	UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII]. HAMAP MF_00277
Sequence similarities	Belongs to the glnD family.

Ontologies

Keywords
Biological process	Nitrogen fixation
Molecular function	Nucleotidyltransferase Transferase
Gene Ontology (GO)
Biological process	nitrogen fixation Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	[protein-PII] uridylyltransferase activity Inferred from electronic annotation. Source: HAMAP amino acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 933	933	[Protein-PII] uridylyltransferase HAMAP MF_00277		PRO_0000192716

Sequences

	Sequence		Length	Mass (Da)
	Q8RQD1-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 35E692E0411BB9E7	FASTA	933	104,602
	10 20 30 40 50 60 MLSTRAASAD ASDAKDAGTA NIPNKRAILS RRKLAEDLET LVAEHGTGDK LRPALIARLR 70 80 90 100 110 120 GALNDGRAEV RARFEAKGSG EDCVRQNCYL ADGVVRSLAD LTVTHIFPTP NPTSGEVFDI 130 140 150 160 170 180 VATGGYGRGE LAPFSDIDLL FLLPYKRTPR VEQVVEYMLY ILWDLGLKVG HAVRSVDDCI 190 200 210 220 230 240 RQSKADVTIR TAILESRYLW GPRKLFHRLR RRFDREVVAG TGPEFVEAKL AERDNRHLKL 250 260 270 280 290 300 GDSAYVLEPN LKDGKGGLRD LQTLFWIAKY LYRVEDVDDL VGKKVLLPEE AHGFAKAQNF 310 320 330 340 350 360 LWTARCHLHY LTGRMEDRMT FDVQTSIGNR MGYTDHAGTK GVERFMKHYF LVAKDVGDLT 370 380 390 400 410 420 RIFCAALEAE SKRPPKFNIL RLAALARRKD VDGFVVDGER LNVRSDRQFK DEPLDMIRLF 430 440 450 460 470 480 HTAQQNDIDI HPNALRAITR SLSVVGPKLR ADPEANRLFL EILTGRKDPE ITLRRMNEAG 490 500 510 520 530 540 VLARFIPDFG RVVAQMQYDM YHVYTVDEHT LFALGILHKI EMGELTDELP LSSEVIHKVV 550 560 570 580 590 600 SRRALYVAVL LHDIAKGRGG DHSILGARVA EKLCPRLGLT AEETETVAWL VRWHLAMSYT 610 620 630 640 650 660 AFKRDLEDDK TVRDFVSLVQ SPERLRLLLV LTVADIRAVG PQRWNNWKAT LLRELYNRSE 670 680 690 700 710 720 EVMSGGLSVE GRGRRIQAAQ AALRDELSDF DAADFERHLA LGYPAYWLAF DAETLGRQAR 730 740 750 760 770 780 LVRGRLRDER PLTVNTRIDR GRAITEVTIF ATDHHGLFSR LAGALAAAGA DIVDARIFTM 790 800 810 820 830 840 TNGMALDVFT VQDAAGGGAF ESGDKLAKLS VMIEKVLSGQ LKPLHDLTKR KAPHASRTRV 850 860 870 880 890 900 FHVPPRVLID NNASTTHTVI EVNGRDRPGL LYDLTRALTN LTLQISSAKI STYGEKAIDV 910 920 930 FYVKDVFGLK VTHENKLAQI RERLLHALAD PSA

References

[1]	"Cloning and characterization of the Azospirillum brasilense glnD gene and analysis of a glnD mutant." Van Dommelen A., Keijers V., Somers E., Vanderleyden J. Mol. Gen. Genet. 266:813-820(2002) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 29145 / Sp7 / DSM 1690 / IMET 11303.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF149716 Genomic DNA. Translation: AAL87737.1.
3D structure databases
SMR	Q8RQD1. Positions 76-358, 401-648.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.7.59. 1315.
Family and domain databases
HAMAP	MF_00277. PII_uridylyl-transf. [Tree]
InterPro	IPR002912. ACT_bd. IPR010043. GlnD_Uridyltrans. IPR003607. Metal-dep_PHydrolase_HD_dom. IPR006674. Metal-dep_PHydrolase_HD_sub. IPR002934. Nucleotidyltransferase. IPR013546. PII_UdlTrfase/GS_AdlTrfase. [Graphical view]
PANTHER	PTHR13734:SF1. GlnD_Uridyltrans. 1 hit.
Pfam	PF01842. ACT. 2 hits. PF08335. GlnD_UR_UTase. 1 hit. PF01966. HD. 1 hit. PF01909. NTP_transf_2. 1 hit. [Graphical view]
PIRSF	PIRSF006288. PII_uridyltransf. 1 hit.
SMART	SM00471. HDc. 1 hit. [Graphical view]
TIGRFAMs	TIGR01693. UTase_glnD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLND_AZOBR

Accession

Primary (citable) accession number: Q8RQD1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 23, 2003
Last sequence update:	June 1, 2002
Last modified:	February 9, 2010
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)