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Protein

Cytochrome P450 165B3

Gene

cyp165B3

Organism
Amycolatopsis orientalis (Nocardia orientalis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the coupling of aromatic side chains of the heptapeptide of vancomycin.

Cofactori

Pathwayi: vancomycin biosynthesis

This protein is involved in the pathway vancomycin biosynthesis, which is part of Antibiotic biosynthesis.
View all proteins of this organism that are known to be involved in the pathway vancomycin biosynthesis and in Antibiotic biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi347 – 3471Iron (heme axial ligand)

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 165B3 (EC:1.14.-.-)
Alternative name(s):
Vancomycin biosynthesis protein OxyB
Gene namesi
Name:cyp165B3
Synonyms:oxyB
OrganismiAmycolatopsis orientalis (Nocardia orientalis)Imported
Taxonomic identifieri31958 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaPseudonocardialesPseudonocardiaceaeAmycolatopsis

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL1075039.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 398398Cytochrome P450 165B3PRO_0000052239Add
BLAST

Interactioni

Protein-protein interaction databases

DIPiDIP-61594N.

Chemistry

BindingDBiQ8RN04.

Structurei

Secondary structure

1
398
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi9 – 113Combined sources
Helixi20 – 234Combined sources
Beta strandi27 – 315Combined sources
Turni36 – 383Combined sources
Beta strandi42 – 454Combined sources
Helixi48 – 569Combined sources
Turni58 – 603Combined sources
Beta strandi61 – 633Combined sources
Helixi88 – 903Combined sources
Helixi95 – 1039Combined sources
Helixi104 – 1074Combined sources
Helixi109 – 13325Combined sources
Beta strandi135 – 1384Combined sources
Helixi139 – 1424Combined sources
Turni143 – 1464Combined sources
Helixi147 – 15610Combined sources
Helixi160 – 1623Combined sources
Helixi163 – 17210Combined sources
Helixi180 – 20324Combined sources
Helixi209 – 2179Combined sources
Helixi218 – 2203Combined sources
Helixi223 – 23614Combined sources
Helixi239 – 25416Combined sources
Helixi256 – 2627Combined sources
Helixi266 – 28015Combined sources
Beta strandi289 – 2935Combined sources
Beta strandi295 – 2973Combined sources
Beta strandi300 – 3023Combined sources
Beta strandi307 – 3104Combined sources
Helixi312 – 3154Combined sources
Turni319 – 3213Combined sources
Turni323 – 3264Combined sources
Helixi350 – 36718Combined sources
Beta strandi372 – 3754Combined sources
Beta strandi381 – 3833Combined sources
Beta strandi385 – 3884Combined sources
Beta strandi390 – 3934Combined sources
Beta strandi395 – 3973Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1LFKX-ray1.70A1-398[»]
1LG9X-ray2.00A1-398[»]
1LGFX-ray2.20A1-398[»]
ProteinModelPortaliQ8RN04.
SMRiQ8RN04. Positions 5-398.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8RN04.

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR002397. Cyt_P450_B.
IPR017972. Cyt_P450_CS.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00359. BP450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8RN04-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEDDPRPLH IRRQGLDPAD ELLAAGALTR VTIGSGADAE THWMATAHAV
60 70 80 90 100
VRQVMGDHQQ FSTRRRWDPR DEIGGKGIFR PRELVGNLMD YDPPEHTRLR
110 120 130 140 150
RKLTPGFTLR KMQRMAPYIE QIVNDRLDEM ERAGSPADLI AFVADKVPGA
160 170 180 190 200
VLCELVGVPR DDRDMFMKLC HGHLDASLSQ KRRAALGDKF SRYLLAMIAR
210 220 230 240 250
ERKEPGEGMI GAVVAEYGDD ATDEELRGFC VQVMLAGDDN ISGMIGLGVL
260 270 280 290 300
AMLRHPEQID AFRGDEQSAQ RAVDELIRYL TVPYSPTPRI AREDLTLAGQ
310 320 330 340 350
EIKKGDSVIC SLPAANRDPA LAPDVDRLDV TREPIPHVAF GHGVHHCLGA
360 370 380 390
ALARLELRTV FTELWRRFPA LRLADPAQDT EFRLTTPAYG LTELMVAW
Length:398
Mass (Da):44,367
Last modified:June 1, 2002 - v1
Checksum:iF91FEA7FB0E91783
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF486630 Genomic DNA. Translation: AAL90878.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF486630 Genomic DNA. Translation: AAL90878.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1LFKX-ray1.70A1-398[»]
1LG9X-ray2.00A1-398[»]
1LGFX-ray2.20A1-398[»]
ProteinModelPortaliQ8RN04.
SMRiQ8RN04. Positions 5-398.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-61594N.

Chemistry

BindingDBiQ8RN04.
ChEMBLiCHEMBL1075039.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00162.

Miscellaneous databases

EvolutionaryTraceiQ8RN04.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR002397. Cyt_P450_B.
IPR017972. Cyt_P450_CS.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00359. BP450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Crystal structure of OxyB, a cytochrome P450 implicated in an oxidative phenol coupling reaction during vancomycin biosynthesis."
    Zerbe K., Pylypenko O., Vitali F., Zhang W., Rousett S., Heck M., Vrijbloed J.W., Bischoff D., Bister B., Suessmuth R.D., Pelzer S., Wohlleben W., Robinson J.A., Schlichting I.
    J. Biol. Chem. 277:47476-47485(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS).

Entry informationi

Entry nameiC5B3_AMYOR
AccessioniPrimary (citable) accession number: Q8RN04
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: June 1, 2002
Last modified: January 20, 2016
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.