Reviewed, UniProtKB/Swiss-Prot Q8RMG1 (PROD1_BACNA)

Names and origin

Protein names

Recommended name:
    Proline dehydrogenase 1
      Short name=PRODH 1
    EC=1.5.99.8
Alternative name(s):
    Proline oxidase 1

Gene names

Name:	fadM
Synonyms:	yusM

Organism

Bacillus subtilis subsp. natto

Taxonomic identifier

86029 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	302 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the oxidation of L-proline to delta-1-pyrroline-5-carboxylate (P5C). Ref.1
Catalytic activity	L-proline + acceptor = (S)-1-pyrroline-5-carboxylate + reduced acceptor. Ref.1
Cofactor	FAD By similarity.
Pathway	Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 1/2.
Sequence similarities	Belongs to the proline oxidase family.
Biophysicochemical properties	pH dependence: Optimum pH is 7.5-8. Temperature dependence: Optimum temperature is 25 degrees Celsius. Loses 70% of activity upon activation at 45 degrees Celsius for 15 minutes.

Ontologies

Keywords
Biological process	Proline metabolism
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	glutamate biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	proline dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 302	302	Proline dehydrogenase 1		PRO_0000361667

Sequences

	Sequence		Length	Mass (Da)
	Q8RMG1-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: B39468A37A1C873E	FASTA	302	34,510
	10 20 30 40 50 60 MLRHVFLFLS QNKTLTKFAK AYGTRLGARR FVAGDTIESA VKTVKRLNRS GLCATIDYLG 70 80 90 100 110 120 EYAASEKEAN QVAEECKKAI QAIAEHQLDS ELSLKLTSIG LDLSEELALT HLRAILSVAK 130 140 150 160 170 180 QYDVAVTIDM EDYSHYEQTL SIYRQCKQEF EKLGTVIQAY LYRAAEDIKK MRDLKPNLRL 190 200 210 220 230 240 VKGAYKESAA VAFPDKRGTD LHFQSLIKLQ LLSGNYTAVA THDDDIIKFT KQLVAEHRIP 250 260 270 280 290 300 ASQFEFQMLY GIRPERQKEL AKEGYRMRVY VPYGTDWFSY FMRRIAERPA NAAFVLKGIL KK

References

[1]

"Delta1-pyrroline-5-carboxylic acid formed by proline dehydrogenase from the Bacillus subtilis ssp. natto expressed in Escherichia coli as a precursor for 2-acetyl-1-pyrroline." Huang T.-C., Huang Y.-W., Hung H.-J., Ho C.-T., Wu M.-L. J. Agric. Food Chem. 55:5097-5102(2007) [PubMed: 17536821] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES. Strain: CCRC 14716.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF497244 Genomic DNA. Translation: AAM16152.1.
3D structure databases
HSSP	HSSP built from PDB template 1TJ1 based on UniProtKB P09546.
SMR	Q8RMG1. Positions 2-288.
ModBase	Search...
Family and domain databases
InterPro	IPR015590. Aldehyde_DH. IPR002872. Proline_DH. IPR008219. Proline_DH_prd. [Graphical view]
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF01619. Pro_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000196. Pro_dehydrog. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PROD1_BACNA

Accession

Primary (citable) accession number: Q8RMG1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	June 1, 2002
Last modified:	January 19, 2010
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)