Reviewed, UniProtKB/Swiss-Prot Q8RKI6 (TARD_BACSU)

Names and origin

Protein names

Recommended name:
    Glycerol-3-phosphate cytidylyltransferase
      Short name=GCT
      Short name=Gro-PCT
    EC=2.7.7.39
Alternative name(s):
    CDP-glycerol pyrophosphorylase

Gene names

Name:

tarD

Organism

Bacillus subtilis

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	129 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides activated glycerol phosphate for teichoic acid synthesis, via incorporation into the linkage unit by tarB and tarF Probable.
Catalytic activity	CTP + sn-glycerol 3-phosphate = diphosphate + CDP-glycerol.
Cofactor	Divalent metal cations. Prefers cobalt, magnesium, manganese or iron By similarity.
Pathway	Cell wall biogenesis; poly(ribitol phosphate) teichoic acid biosynthesis.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the cytidylyltransferase family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation Teichoic acid biosynthesis
Cellular component	Cytoplasm
Ligand	Cobalt Iron Magnesium Manganese
Molecular function	Nucleotidyltransferase Transferase
Gene Ontology (GO)
Biological process	cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW teichoic acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cobalt ion binding Inferred from electronic annotation. Source: UniProtKB-KW glycerol-3-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 129	129	Glycerol-3-phosphate cytidylyltransferase		PRO_0000208467
Sites
	Active site	14	1	By similarity
	Active site	17	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8RKI6-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: F2317D5F8A00247E	FASTA	129	15,257
	10 20 30 40 50 60 MKKVITYGTF DLFHYGHMKL LERAKNLGDY LIVGLSTDEF NLQKQKKSHH SYEHRKFILE 70 80 90 100 110 120 TIDLVNEVIP EKNWEQKISD IQKHDIDTFV IGDDWKGKFD FLKEYCEVIY LPRTDGISTT QIKKDMASL

References

[1]

"Comparison of ribitol and glycerol teichoic acid genes in Bacillus subtilis W23 and 168: identical function, similar divergent organization, but different regulation." Lazarevic V., Abellan F.-X., Beggah Moeller S., Karamata D., Maueel C. Microbiology 148:815-824(2002) [PubMed: 11882717] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROBABLE FUNCTION. Strain: W23.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ313428 Genomic DNA. Translation: CAC86112.1.
3D structure databases
HSSP	HSSP built from PDB template 1COZ based on UniProtKB P27623.
SMR	Q8RKI6. Positions 1-126.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.7.39. 150.
Family and domain databases
InterPro	IPR004821. Cyt_trans_rel. IPR004820. Cytidylyltransf. IPR006409. G3P_cytidyltrfase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF01467. CTP_transf_2. 1 hit. [Graphical view]
TIGRFAMs	TIGR00125. cyt_tran_rel. 1 hit. TIGR01518. g3p_cytidyltrns. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TARD_BACSU

Accession

Primary (citable) accession number: Q8RKI6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2003
Last sequence update:	June 1, 2002
Last modified:	June 16, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)