Q8RJP2 (RHIE_DICD3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Rhamnogalacturonate lyase
Short name=Rhamnogalacturonase
EC=4.2.2.-

Gene names

Name:	rhiE
Ordered Locus Names:	Dda3937_01465

Organism

Dickeya dadantii (strain 3937) (Erwinia chrysanthemi (strain 3937)) [Complete proteome] [HAMAP]

Taxonomic identifier

198628 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Dickeya

Protein attributes

Sequence length	578 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Degrades the rhamnogalacturonan I (RG-I) backbone of pectin. Is required for the full virulence of E.chrysanthemi strain 3937 as it is involved in rotting of plant tissue.
Catalytic activity	Cleaves the alpha-1,4 linkage of RG-I backbone between a rhamnose and a galacturonate, thereby creating an unsaturated bond between carbons 4 and 5 of the galacturonate residue.
Subcellular location	Secreted. Note: Via the type II out secretion pathway.
Induction	By rhamnose.
Miscellaneous	Contrary to other proteins secreted by the Out pathway, rhiE contains no disulfide bond.
Sequence similarities	Belongs to the polysaccharide lyase 4 family.
Caution	Both Met-1 and Met-3 seem to be able to act as initiation codons.
Biophysicochemical properties	pH dependence: Optimum pH is 6.0.

Ontologies

Keywords
Biological process	Virulence
Cellular component	Secreted
Domain	Signal
Molecular function	Lyase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	carbohydrate binding Inferred from electronic annotation. Source: InterPro carboxypeptidase activity Inferred from electronic annotation. Source: InterPro lyase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27
	Chain	28 – 578	551	Rhamnogalacturonate lyase		PRO_0000024914

Sequences

	Sequence		Length	Mass (Da)
	Q8RJP2 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 82B177C96ABA1C47	FASTA	578	64,978
	10 20 30 40 50 60 MHMNKPLQAW RTPLLTLIFV LPLTATGAVK LTLDGMNSTL DNGLLKVRFG ADGSAKEVWK 70 80 90 100 110 120 GGTNLISRLS GAARDPDKNR SFYLDYYSGG VNEFVPERLE VIKQTPDQVH LAYIDDQNGK 130 140 150 160 170 180 LRLEYHLIMT RDVSGLYSYV VAANTGSAPV TVSELRNVYR FDATRLDTLF NSIRRGTPLL 190 200 210 220 230 240 YDELEQLPKV QDETWRLPDG SVYSKYDFAG YQRESRYWGV MGNGYGAWMV PASGEYYSGD 250 260 270 280 290 300 ALKQELLVHQ DAIILNYLTG SHFGTPDMVA QPGFEKLYGP WLLYINQGND RELVADVSRR 310 320 330 340 350 360 AEHERASWPY RWLDDARYPR QRATVSGRLR TEAPHATVVL NSSAENFDIQ TTGYLFSART 370 380 390 400 410 420 NRDGRFSLSN VPPGEYRLSA YADGGTQIGL LAQQTVRVEG KKTRLGQIDA RQPAPLAWAI 430 440 450 460 470 480 GQADRRADEF RFGDKPRQYR WQTEVPADLT FEIGKSRERK DWYYAQTQPG SWHILFNTRT 490 500 510 520 530 540 PEQPYTLNIA IAAASNNGMT TPASSPQLAV KLNGQLLTTL KYDNDKSIYR GAMQSGRYHE 550 560 570 AHIPLPAGAL QQGGNRITLE LLGGMVMYDA ITLTETPQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Rhamnogalacturonate lyase RhiE is secreted by the out system in Erwinia chrysanthemi." Laatu M., Condemine G. J. Bacteriol. 185:1642-1649(2003) [PubMed: 12591882] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF N-TERMINUS, CHARACTERIZATION. Strain: 3937.
[2]	"Complete genome sequence of Dickeya dadantii 3937." International Erwinia Consortium Submitted (SEP-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 3937.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ438339 Genomic DNA. Translation: CAD27359.1. CP002038 Genomic DNA. Translation: ADM97186.1.
RefSeq	YP_003881743.1. NC_014500.1.
3D structure databases
ProteinModelPortal	Q8RJP2.
ModBase	Search...
Protein family/group databases
CAZy	PL4. Polysaccharide Lyase Family 4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	9732388.
GenomeReviews	Gene locus Dda3937_01465 in contig CP002038_GR.
KEGG	ddd:Dda3937_01465.
PATRIC	42314307. VBIDicDad25310_0986.
Organism-specific databases
CMR	Search...
Family and domain databases
InterPro	IPR013784. Carb-bd-like_fold. IPR014766. CarboxyPept_regulatory_dom. IPR008979. Galactose-bd-like. IPR011013. Glyco_hydro-type_carb-bd. IPR010325. Rhamnogal_lyase. [Graphical view]
Gene3D	G3DSA:2.60.40.1120. CarboxyPept_regulatory. 1 hit.
Pfam	PF06045. Rhamnogal_lyase. 1 hit. [Graphical view]
SUPFAM	SSF49452. CBD_4. 1 hit. SSF49785. Gal_bind_like. 1 hit. SSF74650. Gal_mut_like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RHIE_DICD3

Accession

Primary (citable) accession number: Q8RJP2
Secondary accession number(s): E0SBN8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 28, 2003
Last sequence update:	June 1, 2002
Last modified:	January 25, 2012
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program