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Protein
Submitted name:

Peptide amidase

Gene

pam

Organism
Stenotrophomonas maltophilia (Pseudomonas maltophilia) (Xanthomonas maltophilia)
Status
Unreviewed-Annotation score: Annotation score: 1 out of 5-Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei123 – 1231Important for catalytic activityCombined sources
Active sitei202 – 2021Proton donor/acceptorCombined sources
Active sitei226 – 2261NucleophileCombined sources

GO - Molecular functioni

  1. carbon-nitrogen ligase activity, with glutamine as amido-N-donor Source: InterPro
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Submitted name:
Peptide amidaseImported
Gene namesi
Name:pamImported
OrganismiStenotrophomonas maltophilia (Pseudomonas maltophilia) (Xanthomonas maltophilia)Imported
Taxonomic identifieri40324 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeStenotrophomonasStenotrophomonas maltophilia group

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3737 PotentialImportedAdd
BLAST
Chaini38 – 540503peptide amidaseImportedPRO_5000067990Add
BLAST

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1M21X-ray1.80A/B38-540[»]
1M22X-ray1.40A/B38-540[»]
SMRiQ8RJN5. Positions 43-529.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8RJN5.

Family & Domainsi

Keywords - Domaini

SignalImported

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
InterProiIPR000120. Amidase.
IPR023631. Amidase_dom.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8RJN5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRPSLPPLLT CLLAALPALL SAGCSPATPS AHAAEPASRN VPFPYAETDV
60 70 80 90 100
ADLQARMTAG ELDSTTLTQA YLQRIAALDR TGPRLRAVIE LNPDALKEAA
110 120 130 140 150
ERDRERRDGR LRGPLHGIPL LLKDNINAAP MATSAGSLAL QGFRPDDAYL
160 170 180 190 200
VRRLRDAGAV VLGKTNLSEW ANFRGNDSIS GWSARGGQTR NPYRISHSPC
210 220 230 240 250
GSSSGSAVAV AANLASVAIG TETDGSIVCP AAINGVVGLK PTVGLVSRDG
260 270 280 290 300
IIPISFSQDT AGPMARSVAD AAAVLTAIAG RDDADPATAT MPGRAVYDYT
310 320 330 340 350
ARLDPQGLRG KRIGLLQTPL LKYRGMPPLI EQAATELRRA GAVVVPVELP
360 370 380 390 400
NQGAWAEAER TLLLYEFKAG LERYFNTHRA PLRSLADLIA FNQAHSKQEL
410 420 430 440 450
GLFGQELLVE ADATAGLADP AYIRARSDAR RLAGPEGIDA ALAAHQLDAL
460 470 480 490 500
VAPTTGVAWP IRSEGDDFPG ESYSAAAVAG YPSLTVPMGQ IDGLPVGLLF
510 520 530 540
MGTAWSEPKL IEMAYAYEQR TRARRPPHFD TDALIDAGEP
Length:540
Mass (Da):57,080
Last modified:May 31, 2002 - v1
Checksum:iABBA31F903C3EA02
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ414049 Genomic DNA. Translation: CAC93616.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ414049 Genomic DNA. Translation: CAC93616.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1M21X-ray1.80A/B38-540[»]
1M22X-ray1.40A/B38-540[»]
SMRiQ8RJN5. Positions 43-529.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiQ8RJN5.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
InterProiIPR000120. Amidase.
IPR023631. Amidase_dom.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Gene cloning, overexpression and biochemical characterization of the peptide amidase from Stenotrophomonas maltophilia."
    Neumann S., Kula M.-R.
    Appl. Microbiol. Biotechnol. 58:772-780(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: DSM 9181Imported.
  2. "An alternative mechanism for amidase signature enzymes."
    Labahn J., Neumann S., Buldt G., Kula M.R., Granzin J.
    J. Mol. Biol. 322:1053-1064(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.40 ANGSTROMS) OF 38-540, ACTIVE SITE.

Entry informationi

Entry nameiQ8RJN5_STEMA
AccessioniPrimary (citable) accession number: Q8RJN5
Entry historyi
Integrated into UniProtKB/TrEMBL: May 31, 2002
Last sequence update: May 31, 2002
Last modified: March 3, 2015
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.