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Q8RJN5

- Q8RJN5_STEMA

UniProt

Q8RJN5 - Q8RJN5_STEMA

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Protein
Submitted name:

Peptide amidase

Gene

pam

Organism
Stenotrophomonas maltophilia (Pseudomonas maltophilia) (Xanthomonas maltophilia)
Status
Unreviewed - Annotation score: 1 out of 5- Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei123 – 1231Important for catalytic activityImported
Active sitei202 – 2021Proton donor/acceptorImported
Active sitei226 – 2261NucleophileImported

GO - Molecular functioni

  1. carbon-nitrogen ligase activity, with glutamine as amido-N-donor Source: InterPro
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Submitted name:
Peptide amidaseImported
Gene namesi
Name:pamImported
OrganismiStenotrophomonas maltophilia (Pseudomonas maltophilia) (Xanthomonas maltophilia)Imported
Taxonomic identifieri40324 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeStenotrophomonasStenotrophomonas maltophilia group

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3737 PotentialImportedAdd
BLAST
Chaini38 – 540503peptide amidaseImportedPRO_5000067990Add
BLAST

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1M21X-ray1.80A/B38-540[»]
1M22X-ray1.40A/B38-540[»]
ProteinModelPortaliQ8RJN5.
SMRiQ8RJN5. Positions 43-529.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8RJN5.

Family & Domainsi

Keywords - Domaini

SignalImported

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
InterProiIPR000120. Amidase.
IPR023631. Amidase_dom.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8RJN5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRPSLPPLLT CLLAALPALL SAGCSPATPS AHAAEPASRN VPFPYAETDV
60 70 80 90 100
ADLQARMTAG ELDSTTLTQA YLQRIAALDR TGPRLRAVIE LNPDALKEAA
110 120 130 140 150
ERDRERRDGR LRGPLHGIPL LLKDNINAAP MATSAGSLAL QGFRPDDAYL
160 170 180 190 200
VRRLRDAGAV VLGKTNLSEW ANFRGNDSIS GWSARGGQTR NPYRISHSPC
210 220 230 240 250
GSSSGSAVAV AANLASVAIG TETDGSIVCP AAINGVVGLK PTVGLVSRDG
260 270 280 290 300
IIPISFSQDT AGPMARSVAD AAAVLTAIAG RDDADPATAT MPGRAVYDYT
310 320 330 340 350
ARLDPQGLRG KRIGLLQTPL LKYRGMPPLI EQAATELRRA GAVVVPVELP
360 370 380 390 400
NQGAWAEAER TLLLYEFKAG LERYFNTHRA PLRSLADLIA FNQAHSKQEL
410 420 430 440 450
GLFGQELLVE ADATAGLADP AYIRARSDAR RLAGPEGIDA ALAAHQLDAL
460 470 480 490 500
VAPTTGVAWP IRSEGDDFPG ESYSAAAVAG YPSLTVPMGQ IDGLPVGLLF
510 520 530 540
MGTAWSEPKL IEMAYAYEQR TRARRPPHFD TDALIDAGEP
Length:540
Mass (Da):57,080
Last modified:June 1, 2002 - v1
Checksum:iABBA31F903C3EA02
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ414049 Genomic DNA. Translation: CAC93616.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ414049 Genomic DNA. Translation: CAC93616.1 .

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1M21 X-ray 1.80 A/B 38-540 [» ]
1M22 X-ray 1.40 A/B 38-540 [» ]
ProteinModelPortali Q8RJN5.
SMRi Q8RJN5. Positions 43-529.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei Q8RJN5.

Family and domain databases

Gene3Di 3.90.1300.10. 1 hit.
InterProi IPR000120. Amidase.
IPR023631. Amidase_dom.
[Graphical view ]
PANTHERi PTHR11895. PTHR11895. 1 hit.
Pfami PF01425. Amidase. 1 hit.
[Graphical view ]
SUPFAMi SSF75304. SSF75304. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Gene cloning, overexpression and biochemical characterization of the peptide amidase from Stenotrophomonas maltophilia."
    Neumann S., Kula M.-R.
    Appl. Microbiol. Biotechnol. 58:772-780(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: DSM 9181Imported.
  2. "An alternative mechanism for amidase signature enzymes."
    Labahn J., Neumann S., Buldt G., Kula M.R., Granzin J.
    J. Mol. Biol. 322:1053-1064(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.40 ANGSTROMS) OF 38-540, ACTIVE SITE.

Entry informationi

Entry nameiQ8RJN5_STEMA
AccessioniPrimary (citable) accession number: Q8RJN5
Entry historyi
Integrated into UniProtKB/TrEMBL: June 1, 2002
Last sequence update: June 1, 2002
Last modified: October 29, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureImported

External Data

Dasty 3