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Q8RJN5

- Q8RJN5_STEMA

UniProt

Q8RJN5 - Q8RJN5_STEMA

Protein
Submitted name:

Peptide amidase

Gene

pam

Organism
Stenotrophomonas maltophilia (Pseudomonas maltophilia) (Xanthomonas maltophilia)
Status
Unreviewed - Annotation score: 1 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 57 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei123 – 1231Important for catalytic activityImported
    Active sitei202 – 2021Proton donor/acceptorImported
    Active sitei226 – 2261NucleophileImported

    GO - Molecular functioni

    1. carbon-nitrogen ligase activity, with glutamine as amido-N-donor Source: InterPro

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    Peptide amidaseImported
    Gene namesi
    Name:pamImported
    OrganismiStenotrophomonas maltophilia (Pseudomonas maltophilia) (Xanthomonas maltophilia)Imported
    Taxonomic identifieri40324 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeStenotrophomonasStenotrophomonas maltophilia group

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3737 PotentialImportedAdd
    BLAST
    Chaini38 – 540503peptide amidaseImportedPRO_5000067990Add
    BLAST

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1M21X-ray1.80A/B38-540[»]
    1M22X-ray1.40A/B38-540[»]
    ProteinModelPortaliQ8RJN5.
    SMRiQ8RJN5. Positions 43-529.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ8RJN5.

    Family & Domainsi

    Keywords - Domaini

    SignalImported

    Family and domain databases

    Gene3Di3.90.1300.10. 1 hit.
    InterProiIPR000120. Amidase.
    IPR023631. Amidase_dom.
    [Graphical view]
    PANTHERiPTHR11895. PTHR11895. 1 hit.
    PfamiPF01425. Amidase. 1 hit.
    [Graphical view]
    SUPFAMiSSF75304. SSF75304. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8RJN5-1 [UniParc]FASTAAdd to Basket

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    MRPSLPPLLT CLLAALPALL SAGCSPATPS AHAAEPASRN VPFPYAETDV    50
    ADLQARMTAG ELDSTTLTQA YLQRIAALDR TGPRLRAVIE LNPDALKEAA 100
    ERDRERRDGR LRGPLHGIPL LLKDNINAAP MATSAGSLAL QGFRPDDAYL 150
    VRRLRDAGAV VLGKTNLSEW ANFRGNDSIS GWSARGGQTR NPYRISHSPC 200
    GSSSGSAVAV AANLASVAIG TETDGSIVCP AAINGVVGLK PTVGLVSRDG 250
    IIPISFSQDT AGPMARSVAD AAAVLTAIAG RDDADPATAT MPGRAVYDYT 300
    ARLDPQGLRG KRIGLLQTPL LKYRGMPPLI EQAATELRRA GAVVVPVELP 350
    NQGAWAEAER TLLLYEFKAG LERYFNTHRA PLRSLADLIA FNQAHSKQEL 400
    GLFGQELLVE ADATAGLADP AYIRARSDAR RLAGPEGIDA ALAAHQLDAL 450
    VAPTTGVAWP IRSEGDDFPG ESYSAAAVAG YPSLTVPMGQ IDGLPVGLLF 500
    MGTAWSEPKL IEMAYAYEQR TRARRPPHFD TDALIDAGEP 540
    Length:540
    Mass (Da):57,080
    Last modified:June 1, 2002 - v1
    Checksum:iABBA31F903C3EA02
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ414049 Genomic DNA. Translation: CAC93616.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ414049 Genomic DNA. Translation: CAC93616.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1M21 X-ray 1.80 A/B 38-540 [» ]
    1M22 X-ray 1.40 A/B 38-540 [» ]
    ProteinModelPortali Q8RJN5.
    SMRi Q8RJN5. Positions 43-529.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei Q8RJN5.

    Family and domain databases

    Gene3Di 3.90.1300.10. 1 hit.
    InterProi IPR000120. Amidase.
    IPR023631. Amidase_dom.
    [Graphical view ]
    PANTHERi PTHR11895. PTHR11895. 1 hit.
    Pfami PF01425. Amidase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF75304. SSF75304. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Gene cloning, overexpression and biochemical characterization of the peptide amidase from Stenotrophomonas maltophilia."
      Neumann S., Kula M.-R.
      Appl. Microbiol. Biotechnol. 58:772-780(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: DSM 9181Imported.
    2. "An alternative mechanism for amidase signature enzymes."
      Labahn J., Neumann S., Buldt G., Kula M.R., Granzin J.
      J. Mol. Biol. 322:1053-1064(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.40 ANGSTROMS) OF 38-540, ACTIVE SITE.

    Entry informationi

    Entry nameiQ8RJN5_STEMA
    AccessioniPrimary (citable) accession number: Q8RJN5
    Entry historyi
    Integrated into UniProtKB/TrEMBL: June 1, 2002
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 57 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported

    External Data

    Dasty 3