Reviewed, UniProtKB/Swiss-Prot Q8RHJ2 (HPPA_FUSNN)

Names and origin

Protein names

Recommended name:
    Pyrophosphate-energized proton pump
    EC=3.6.1.1
Alternative name(s):
    Pyrophosphate-energized inorganic pyrophosphatase
      Short name=H(+)-PPase
    Membrane-bound proton-translocating pyrophosphatase

Gene names

Name:	hppA
Ordered Locus Names:	FN2030

Organism

Fusobacterium nucleatum subsp. nucleatum [Complete proteome] [HAMAP]

Taxonomic identifier

76856 [NCBI]

Taxonomic lineage

Bacteria › Fusobacteria › Fusobacterales › Fusobacteriaceae › Fusobacterium

Protein attributes

Sequence length	671 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Generates a proton motive force; it probably catalyzes a fully reversible reaction, thus being able to synthesize pyrophosphate when the proton motive force is sufficient By similarity.
Catalytic activity	Diphosphate + H2O = 2 phosphate. HAMAP MF_01129
Cofactor	Magnesium By similarity. Potassium By similarity.
Subunit structure	Homodimer Potential.
Subcellular location	Cell membrane; Multi-pass membrane protein Probable.
Sequence similarities	Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. Type 1 subfamily. [View classification]

Ontologies

Keywords
Biological process	Hydrogen ion transport Ion transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	Magnesium Potassium
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proton transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	hydrogen-translocating pyrophosphatase activity Inferred from electronic annotation. Source: InterPro inorganic diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP potassium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 671	671	Pyrophosphate-energized proton pump HAMAP MF_01129		PRO_0000217003
Regions
	Transmembrane	4 – 26	23	Potential
	Transmembrane	60 – 94	35	Potential
	Transmembrane	126 – 148	23	Potential
	Transmembrane	161 – 183	23	Potential
	Transmembrane	225 – 247	23	Potential
	Transmembrane	254 – 276	23	Potential
	Transmembrane	291 – 313	23	Potential
	Transmembrane	315 – 337	23	Potential
	Transmembrane	361 – 383	23	Potential
	Transmembrane	390 – 412	23	Potential
	Transmembrane	450 – 472	23	Potential
	Transmembrane	493 – 515	23	Potential
	Transmembrane	560 – 579	20	Potential
	Transmembrane	581 – 603	23	Potential
	Transmembrane	646 – 668	23	Potential
Sites
	Site	450	1	Determinant of potassium dependence By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8RHJ2-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: B9519DE6D3554ACB	FASTA	671	68,989
	10 20 30 40 50 60 MDLLTQVMYI GIAVGIISLL AAFYYAKKVE HYQINIPKVE EITAAIREGA MAFLAAEYKI 70 80 90 100 110 120 LIVFVIVVAV ALGIFISVPT AGAFILGAIT SAIAGNAGMR IATKANGRTA IAAKEGGLAK 130 140 150 160 170 180 ALNVAFSGGA VMGLTVVGLG MFMLSLILLV SRTVGISVND VTGFGMGASS IALFARVGGG 190 200 210 220 230 240 IYTKAADVGA DLVGKVEAGI PEDDPRNPAT IADNVGDNVG DVAGMGADLF ESYVGSIIAT 250 260 270 280 290 300 ITLAFLLPVD DATPYVAAPL LISAFGIISS IIATLTVKTD DGSKVHAKLE MGTRIAGILT 310 320 330 340 350 360 IIASFGIIKY LGLDMGIFYA IVAGLVAGLV IAYFTGVYTD TGRRAVNRVS DAAGTGAATA 370 380 390 400 410 420 IIEGLAIGME STVAPLIVIA IAIIISFKTG GLYGISIAAV GMLATTGMVV AVDAYGPVAD 430 440 450 460 470 480 NAGGIAEMSE LPPEVRETTD KLDAVGNSTA AVGKGFAIGS AALTALSLFA AYKEAVDKLT 490 500 510 520 530 540 SEALVIDVTD PEVIAGLFIG GMLTFLFSAL TMTAVGKAAI EMVEEVRRQF REFPGIMDRT 550 560 570 580 590 600 QKPDYKRCVE ISTHSSLKQM ILPGVLAIIV PVIIGLWSVK ALGGLLAGAL VTGVLMAIMM 610 620 630 640 650 660 ANAGGAWDNG KKQIEGGYKG DKKGSDRHKA AVVGDTVGDP FKDTSGPSLN ILIKLMSIVS 670 LVLVPLFVKF M

References

[1]

"Genome sequence and analysis of the oral bacterium Fusobacterium nucleatum strain ATCC 25586." Kapatral V., Anderson I., Ivanova N., Reznik G., Los T., Lykidis A., Bhattacharyya A., Bartman A., Gardner W., Grechkin G., Zhu L., Vasieva O., Chu L., Kogan Y., Chaga O., Goltsman E., Bernal A., Larsen N. , D'Souza M., Walunas T., Pusch G., Haselkorn R., Fonstein M., Kyrpides N.C., Overbeek R. J. Bacteriol. 184:2005-2018(2002) [PubMed: 11889109] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE009951 Genomic DNA. Translation: AAL94115.1.
RefSeq	NP_602816.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	992205.
GenomeReviews	Gene locus FN2030 in contig AE009951_GR.
KEGG	fnu:FN2030.
NMPDR	fig|190304.1.peg.495.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8RHJ2.
OMA	PYLFGGI.
Enzyme and pathway databases
BioCyc	FNUC190304:FN2030-MON.
BRENDA	3.6.1.1. 289963.
Family and domain databases
HAMAP	MF_01129. [Tree]
InterPro	IPR004131. H_PPase. [Graphical view]
Pfam	PF03030. H_PPase. 1 hit. [Graphical view]
PIRSF	PIRSF001265. H+-PPase. 1 hit.
TIGRFAMs	TIGR01104. V_PPase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HPPA_FUSNN

Accession

Primary (citable) accession number: Q8RHJ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2003
Last sequence update:	June 1, 2002
Last modified:	November 3, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)