Q8RHF4 (FTHS_FUSNN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Formate--tetrahydrofolate ligase
EC=6.3.4.3

Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS

Gene names

Name:	fhs
Ordered Locus Names:	FN2082

Organism

Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131) [Reference proteome] [HAMAP]

Taxonomic identifier

190304 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Fusobacteria › Fusobacteriia › Fusobacteriales › Fusobacteriaceae › Fusobacterium › Fusobacterium nucleatum › Fusobacterium nucleatum subsp. nucleatum

Protein attributes

Sequence length	544 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP-Rule MF_01543
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_01543
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	folic acid-containing compound biosynthetic process Inferred from electronic annotation. Source: InterPro tetrahydrofolate interconversion Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 544	544	Formate--tetrahydrofolate ligase HAMAP-Rule MF_01543		PRO_0000199349
Regions
	Nucleotide binding	63 – 70	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8RHF4 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: C3CAEF3D15625B75	FASTA	544	58,321
	10 20 30 40 50 60 MTDIQIAQAA KKENIVEIAK KLGLTEDDIE QYGKYKAKVN LDVLQKNKRP NGKLILVTAI 70 80 90 100 110 120 TPTPAGEGKS TVTIGLTQAL NKMGKLSAAA IREPSLGPVF GMKGGAAGGG YAQVVPMEDI 130 140 150 160 170 180 NLHFTGDMHA IGIAHNLISA CIDNHINSGN ALGIDVTKIT WKRVVDMNDR ALRNIVIGLG 190 200 210 220 230 240 GKANGYPRQD SFQITVGSEI MAILCLSNSI TELKEKIKNI VIGTSVTGKL IKVGDFHIEG 250 260 270 280 290 300 AVAALLKDAI KPNLVQTLEN TPVFIHGGPF ANIAHGCNSI LATKMALKLT DYVVTEAGFA 310 320 330 340 350 360 ADLGAEKFID IKCRLGGLKP DCAVIVATVR ALEHHGKGDL KAGLENLDKH IDNIKNKYKL 370 380 390 400 410 420 PLVVAINKFI TDTDEQINMI EKFCNERGAE VSLCEVWAKG GEGGIDLAEK VLKAIDNNKT 430 440 450 460 470 480 EFDYFYDINL TIKEKIEKIC KEIYGADGVI FAPATKKVFD VIEAEGLNKL PVCMSKTQKS 490 500 510 520 530 540 ISDNPALLGK PTGFKVTIND LRLAVGAGFV IAMAGDIIDM PGLPKKPSAE VIDIDENGVI SGLF

References

[1]

"Genome sequence and analysis of the oral bacterium Fusobacterium nucleatum strain ATCC 25586." Kapatral V., Anderson I., Ivanova N., Reznik G., Los T., Lykidis A., Bhattacharyya A., Bartman A., Gardner W., Grechkin G., Zhu L., Vasieva O., Chu L., Kogan Y., Chaga O., Goltsman E., Bernal A., Larsen N. , D'Souza M., Walunas T., Pusch G., Haselkorn R., Fonstein M., Kyrpides N.C., Overbeek R. J. Bacteriol. 184:2005-2018(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009951 Genomic DNA. Translation: AAL94166.1.
RefSeq	NP_602867.1. NC_003454.1.
3D structure databases
ProteinModelPortal	Q8RHF4.
SMR	Q8RHF4. Positions 3-542.
ModBase	Search...
Protein-protein interaction databases
STRING	190304.FN2082.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL94166; AAL94166; FN2082.
GeneID	992059.
KEGG	fnu:FN2082.
PATRIC	21949689. VBIFusNuc122357_0544.
Phylogenomic databases
eggNOG	COG2759.
HOGENOM	HOG000040280.
KO	K01938.
OMA	EIMAVLC.
Enzyme and pathway databases
UniPathway	UPA00193.
Family and domain databases
HAMAP	MF_01543. FTHFS.
InterPro	IPR000559. Formate_THF_ligase. IPR020628. Formate_THF_ligase_CS. [Graphical view]
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
PROSITE	PS00721. FTHFS_1. 1 hit. PS00722. FTHFS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS_FUSNN

Accession

Primary (citable) accession number: Q8RHF4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program