Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei565 – 5651Aminoacyl-adenylateUniRule annotation
Binding sitei609 – 6091ATPUniRule annotation
Metal bindingi906 – 9061ZincUniRule annotation
Metal bindingi909 – 9091ZincUniRule annotation
Metal bindingi925 – 9251ZincUniRule annotation
Metal bindingi928 – 9281ZincUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:FN0067
OrganismiFusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131)
Taxonomic identifieri190304 [NCBI]
Taxonomic lineageiBacteriaFusobacteriaFusobacterialesFusobacteriaceaeFusobacterium
ProteomesiUP000002521 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 933933Isoleucine--tRNA ligasePRO_0000098389Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi190304.FN0067.

Structurei

3D structure databases

ProteinModelPortaliQ8RH47.
SMRiQ8RH47. Positions 5-933.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi60 – 7011"HIGH" regionAdd
BLAST
Motifi606 – 6105"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
InParanoidiQ8RH47.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8RH47-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDKEYTSTL HLPKTDFQMK ANLPNKEPKY IKKWTEEKIY EKGLEKNKNG
60 70 80 90 100
ETFILHDGPP YANGNTHIGH ALNKILKDII LKYKTFRGFR SPYVPGWDTH
110 120 130 140 150
GLPIELQVVK EVGVGKAREM SALEIRKLCE KYAKKWVGIQ KEQFIRLGVL
160 170 180 190 200
GDWDNPYLTL DPRFEAKQLE LFGEIYVNGY IFKGLKPVYW SPATETALAE
210 220 230 240 250
AEIEYYDHVS PSIYVRMQAN KDLLDKIGFN EDVYVLIWTT TPWTLPANVA
260 270 280 290 300
ICLNENFDYG LYKTEKGNLI LAKDLAESAF KDIGIENFEL IKEFKGKDLE
310 320 330 340 350
YTTYKHPFLE RTGLIILGDH VTADAGTGAV HTAPGHGQDD YVVGLAYKLP
360 370 380 390 400
VISPIDHRGC LTEEAGDLFK GLVYSEANKA IIKHLTETGH ILKMQEISHS
410 420 430 440 450
YPHDWRSKTP VIFRATEQWF IRMEGGDLRE KTLKVIDKIN FIPSWGKNRI
460 470 480 490 500
GSMMETRPDW CISRQRVWGV PIPIFYNDET NEEIFHKEIL DRICDLVREH
510 520 530 540 550
GSNIWVEKSP EELIGEELLV KYNLKGLKLR KETNIMDVWF DSGSSHRGVL
560 570 580 590 600
EVWEGLHRPC DLYLEGSDQH RGWFHTSLLT SVASTGDSPY KSVLTHGFVN
610 620 630 640 650
DGEGKKMSKS LGNTVSPEDV IKVYGADILR LWCGSVDYRD DVRISDNIVK
660 670 680 690 700
QMSEAYRRIR NTARYILGNS YDFNPKTDKV AYKDMLEIDK WALNKLEVLK
710 720 730 740 750
RSVTESYDKY EFYNLFQGIH YFAAIDMSAF YLDIIKDRLY TEKKDSVARR
760 770 780 790 800
AAQTVMYEVL MTLTKMVAPI LSFTAEEIWE SLPAETRESE SIFLADWYVN
810 820 830 840 850
NDEYLKPELD EKWQQIIKLR KEVNKKLEKA RQGENKIIGN SLDAKVSLYT
860 870 880 890 900
EDNTLKEFIK ENLELLKIVF IVSDLEVVDS ADGNYTDAEE IEKLKIKIAH
910 920 930
ADGEKCERCW KYDELGTDSE HPTLCPRCAA VLK
Length:933
Mass (Da):107,496
Last modified:June 1, 2002 - v1
Checksum:i10058192B01AEECA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009951 Genomic DNA. Translation: AAL94280.1.
RefSeqiNP_602981.1. NC_003454.1.
WP_005903528.1. NC_003454.1.

Genome annotation databases

EnsemblBacteriaiAAL94280; AAL94280; FN0067.
GeneIDi992969.
KEGGifnu:FN0067.
PATRICi21949919. VBIFusNuc122357_0659.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009951 Genomic DNA. Translation: AAL94280.1.
RefSeqiNP_602981.1. NC_003454.1.
WP_005903528.1. NC_003454.1.

3D structure databases

ProteinModelPortaliQ8RH47.
SMRiQ8RH47. Positions 5-933.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi190304.FN0067.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL94280; AAL94280; FN0067.
GeneIDi992969.
KEGGifnu:FN0067.
PATRICi21949919. VBIFusNuc122357_0659.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
InParanoidiQ8RH47.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131.

Entry informationi

Entry nameiSYI_FUSNN
AccessioniPrimary (citable) accession number: Q8RH47
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 1, 2002
Last modified: May 27, 2015
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.