SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q8RH47

- SYI_FUSNN

UniProt

Q8RH47 - SYI_FUSNN

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Isoleucine--tRNA ligase
Gene
ileS, FN0067
Organism
Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei565 – 5651Aminoacyl-adenylate By similarity
Binding sitei609 – 6091ATP By similarity
Metal bindingi906 – 9061Zinc By similarity
Metal bindingi909 – 9091Zinc By similarity
Metal bindingi925 – 9251Zinc By similarity
Metal bindingi928 – 9281Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:FN0067
OrganismiFusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131)
Taxonomic identifieri190304 [NCBI]
Taxonomic lineageiBacteriaFusobacteriaFusobacterialesFusobacteriaceaeFusobacterium
ProteomesiUP000002521: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 933933Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098389Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi190304.FN0067.

Structurei

3D structure databases

ProteinModelPortaliQ8RH47.
SMRiQ8RH47. Positions 5-933.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi60 – 7011"HIGH" regionUniRule annotation
Add
BLAST
Motifi606 – 6105"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8RH47-1 [UniParc]FASTAAdd to Basket

« Hide

MSDKEYTSTL HLPKTDFQMK ANLPNKEPKY IKKWTEEKIY EKGLEKNKNG    50
ETFILHDGPP YANGNTHIGH ALNKILKDII LKYKTFRGFR SPYVPGWDTH 100
GLPIELQVVK EVGVGKAREM SALEIRKLCE KYAKKWVGIQ KEQFIRLGVL 150
GDWDNPYLTL DPRFEAKQLE LFGEIYVNGY IFKGLKPVYW SPATETALAE 200
AEIEYYDHVS PSIYVRMQAN KDLLDKIGFN EDVYVLIWTT TPWTLPANVA 250
ICLNENFDYG LYKTEKGNLI LAKDLAESAF KDIGIENFEL IKEFKGKDLE 300
YTTYKHPFLE RTGLIILGDH VTADAGTGAV HTAPGHGQDD YVVGLAYKLP 350
VISPIDHRGC LTEEAGDLFK GLVYSEANKA IIKHLTETGH ILKMQEISHS 400
YPHDWRSKTP VIFRATEQWF IRMEGGDLRE KTLKVIDKIN FIPSWGKNRI 450
GSMMETRPDW CISRQRVWGV PIPIFYNDET NEEIFHKEIL DRICDLVREH 500
GSNIWVEKSP EELIGEELLV KYNLKGLKLR KETNIMDVWF DSGSSHRGVL 550
EVWEGLHRPC DLYLEGSDQH RGWFHTSLLT SVASTGDSPY KSVLTHGFVN 600
DGEGKKMSKS LGNTVSPEDV IKVYGADILR LWCGSVDYRD DVRISDNIVK 650
QMSEAYRRIR NTARYILGNS YDFNPKTDKV AYKDMLEIDK WALNKLEVLK 700
RSVTESYDKY EFYNLFQGIH YFAAIDMSAF YLDIIKDRLY TEKKDSVARR 750
AAQTVMYEVL MTLTKMVAPI LSFTAEEIWE SLPAETRESE SIFLADWYVN 800
NDEYLKPELD EKWQQIIKLR KEVNKKLEKA RQGENKIIGN SLDAKVSLYT 850
EDNTLKEFIK ENLELLKIVF IVSDLEVVDS ADGNYTDAEE IEKLKIKIAH 900
ADGEKCERCW KYDELGTDSE HPTLCPRCAA VLK 933
Length:933
Mass (Da):107,496
Last modified:June 1, 2002 - v1
Checksum:i10058192B01AEECA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE009951 Genomic DNA. Translation: AAL94280.1.
RefSeqiNP_602981.1. NC_003454.1.

Genome annotation databases

EnsemblBacteriaiAAL94280; AAL94280; FN0067.
GeneIDi992969.
KEGGifnu:FN0067.
PATRICi21949919. VBIFusNuc122357_0659.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE009951 Genomic DNA. Translation: AAL94280.1 .
RefSeqi NP_602981.1. NC_003454.1.

3D structure databases

ProteinModelPortali Q8RH47.
SMRi Q8RH47. Positions 5-933.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 190304.FN0067.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL94280 ; AAL94280 ; FN0067 .
GeneIDi 992969.
KEGGi fnu:FN0067.
PATRICi 21949919. VBIFusNuc122357_0659.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131.

Entry informationi

Entry nameiSYI_FUSNN
AccessioniPrimary (citable) accession number: Q8RH47
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 1, 2002
Last modified: May 14, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi