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Q8RH47

- SYI_FUSNN

UniProt

Q8RH47 - SYI_FUSNN

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei565 – 5651Aminoacyl-adenylateUniRule annotation
    Binding sitei609 – 6091ATPUniRule annotation
    Metal bindingi906 – 9061ZincUniRule annotation
    Metal bindingi909 – 9091ZincUniRule annotation
    Metal bindingi925 – 9251ZincUniRule annotation
    Metal bindingi928 – 9281ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:FN0067
    OrganismiFusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131)
    Taxonomic identifieri190304 [NCBI]
    Taxonomic lineageiBacteriaFusobacteriaFusobacterialesFusobacteriaceaeFusobacterium
    ProteomesiUP000002521: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 933933Isoleucine--tRNA ligasePRO_0000098389Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi190304.FN0067.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8RH47.
    SMRiQ8RH47. Positions 5-933.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi60 – 7011"HIGH" regionAdd
    BLAST
    Motifi606 – 6105"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8RH47-1 [UniParc]FASTAAdd to Basket

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    MSDKEYTSTL HLPKTDFQMK ANLPNKEPKY IKKWTEEKIY EKGLEKNKNG    50
    ETFILHDGPP YANGNTHIGH ALNKILKDII LKYKTFRGFR SPYVPGWDTH 100
    GLPIELQVVK EVGVGKAREM SALEIRKLCE KYAKKWVGIQ KEQFIRLGVL 150
    GDWDNPYLTL DPRFEAKQLE LFGEIYVNGY IFKGLKPVYW SPATETALAE 200
    AEIEYYDHVS PSIYVRMQAN KDLLDKIGFN EDVYVLIWTT TPWTLPANVA 250
    ICLNENFDYG LYKTEKGNLI LAKDLAESAF KDIGIENFEL IKEFKGKDLE 300
    YTTYKHPFLE RTGLIILGDH VTADAGTGAV HTAPGHGQDD YVVGLAYKLP 350
    VISPIDHRGC LTEEAGDLFK GLVYSEANKA IIKHLTETGH ILKMQEISHS 400
    YPHDWRSKTP VIFRATEQWF IRMEGGDLRE KTLKVIDKIN FIPSWGKNRI 450
    GSMMETRPDW CISRQRVWGV PIPIFYNDET NEEIFHKEIL DRICDLVREH 500
    GSNIWVEKSP EELIGEELLV KYNLKGLKLR KETNIMDVWF DSGSSHRGVL 550
    EVWEGLHRPC DLYLEGSDQH RGWFHTSLLT SVASTGDSPY KSVLTHGFVN 600
    DGEGKKMSKS LGNTVSPEDV IKVYGADILR LWCGSVDYRD DVRISDNIVK 650
    QMSEAYRRIR NTARYILGNS YDFNPKTDKV AYKDMLEIDK WALNKLEVLK 700
    RSVTESYDKY EFYNLFQGIH YFAAIDMSAF YLDIIKDRLY TEKKDSVARR 750
    AAQTVMYEVL MTLTKMVAPI LSFTAEEIWE SLPAETRESE SIFLADWYVN 800
    NDEYLKPELD EKWQQIIKLR KEVNKKLEKA RQGENKIIGN SLDAKVSLYT 850
    EDNTLKEFIK ENLELLKIVF IVSDLEVVDS ADGNYTDAEE IEKLKIKIAH 900
    ADGEKCERCW KYDELGTDSE HPTLCPRCAA VLK 933
    Length:933
    Mass (Da):107,496
    Last modified:June 1, 2002 - v1
    Checksum:i10058192B01AEECA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE009951 Genomic DNA. Translation: AAL94280.1.
    RefSeqiNP_602981.1. NC_003454.1.

    Genome annotation databases

    EnsemblBacteriaiAAL94280; AAL94280; FN0067.
    GeneIDi992969.
    KEGGifnu:FN0067.
    PATRICi21949919. VBIFusNuc122357_0659.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE009951 Genomic DNA. Translation: AAL94280.1 .
    RefSeqi NP_602981.1. NC_003454.1.

    3D structure databases

    ProteinModelPortali Q8RH47.
    SMRi Q8RH47. Positions 5-933.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 190304.FN0067.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL94280 ; AAL94280 ; FN0067 .
    GeneIDi 992969.
    KEGGi fnu:FN0067.
    PATRICi 21949919. VBIFusNuc122357_0659.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131.

    Entry informationi

    Entry nameiSYI_FUSNN
    AccessioniPrimary (citable) accession number: Q8RH47
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 82 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3