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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei565Aminoacyl-adenylateUniRule annotation1
Binding sitei609ATPUniRule annotation1
Metal bindingi906ZincUniRule annotation1
Metal bindingi909ZincUniRule annotation1
Metal bindingi925ZincUniRule annotation1
Metal bindingi928ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciFNUC190304:G1FZS-680-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:FN0067
OrganismiFusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131)
Taxonomic identifieri190304 [NCBI]
Taxonomic lineageiBacteriaFusobacteriaFusobacterialesFusobacteriaceaeFusobacterium
Proteomesi
  • UP000002521 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000983891 – 933Isoleucine--tRNA ligaseAdd BLAST933

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi190304.FN0067

Structurei

3D structure databases

ProteinModelPortaliQ8RH47
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi60 – 70"HIGH" regionAdd BLAST11
Motifi606 – 610"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
InParanoidiQ8RH47
KOiK01870
OMAiHLGTAWN

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit

Sequencei

Sequence statusi: Complete.

Q8RH47-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDKEYTSTL HLPKTDFQMK ANLPNKEPKY IKKWTEEKIY EKGLEKNKNG
60 70 80 90 100
ETFILHDGPP YANGNTHIGH ALNKILKDII LKYKTFRGFR SPYVPGWDTH
110 120 130 140 150
GLPIELQVVK EVGVGKAREM SALEIRKLCE KYAKKWVGIQ KEQFIRLGVL
160 170 180 190 200
GDWDNPYLTL DPRFEAKQLE LFGEIYVNGY IFKGLKPVYW SPATETALAE
210 220 230 240 250
AEIEYYDHVS PSIYVRMQAN KDLLDKIGFN EDVYVLIWTT TPWTLPANVA
260 270 280 290 300
ICLNENFDYG LYKTEKGNLI LAKDLAESAF KDIGIENFEL IKEFKGKDLE
310 320 330 340 350
YTTYKHPFLE RTGLIILGDH VTADAGTGAV HTAPGHGQDD YVVGLAYKLP
360 370 380 390 400
VISPIDHRGC LTEEAGDLFK GLVYSEANKA IIKHLTETGH ILKMQEISHS
410 420 430 440 450
YPHDWRSKTP VIFRATEQWF IRMEGGDLRE KTLKVIDKIN FIPSWGKNRI
460 470 480 490 500
GSMMETRPDW CISRQRVWGV PIPIFYNDET NEEIFHKEIL DRICDLVREH
510 520 530 540 550
GSNIWVEKSP EELIGEELLV KYNLKGLKLR KETNIMDVWF DSGSSHRGVL
560 570 580 590 600
EVWEGLHRPC DLYLEGSDQH RGWFHTSLLT SVASTGDSPY KSVLTHGFVN
610 620 630 640 650
DGEGKKMSKS LGNTVSPEDV IKVYGADILR LWCGSVDYRD DVRISDNIVK
660 670 680 690 700
QMSEAYRRIR NTARYILGNS YDFNPKTDKV AYKDMLEIDK WALNKLEVLK
710 720 730 740 750
RSVTESYDKY EFYNLFQGIH YFAAIDMSAF YLDIIKDRLY TEKKDSVARR
760 770 780 790 800
AAQTVMYEVL MTLTKMVAPI LSFTAEEIWE SLPAETRESE SIFLADWYVN
810 820 830 840 850
NDEYLKPELD EKWQQIIKLR KEVNKKLEKA RQGENKIIGN SLDAKVSLYT
860 870 880 890 900
EDNTLKEFIK ENLELLKIVF IVSDLEVVDS ADGNYTDAEE IEKLKIKIAH
910 920 930
ADGEKCERCW KYDELGTDSE HPTLCPRCAA VLK
Length:933
Mass (Da):107,496
Last modified:June 1, 2002 - v1
Checksum:i10058192B01AEECA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009951 Genomic DNA Translation: AAL94280.1
RefSeqiNP_602981.1, NC_003454.1
WP_005903528.1, NC_003454.1

Genome annotation databases

EnsemblBacteriaiAAL94280; AAL94280; FN0067
GeneIDi992969
KEGGifnu:FN0067
PATRICifig|190304.8.peg.659

Similar proteinsi

Entry informationi

Entry nameiSYI_FUSNN
AccessioniPrimary (citable) accession number: Q8RH47
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 1, 2002
Last modified: March 28, 2018
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health