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Protein

Crossover junction endodeoxyribonuclease RuvC

Gene

ruvC

Organism
Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi7MagnesiumUniRule annotation1
Metal bindingi67MagnesiumUniRule annotation1
Metal bindingi140MagnesiumUniRule annotation1
Metal bindingi143MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Nuclease
Biological processDNA damage, DNA recombination, DNA repair
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endodeoxyribonuclease RuvCUniRule annotation (EC:3.1.22.4UniRule annotation)
Alternative name(s):
Holliday junction nuclease RuvCUniRule annotation
Holliday junction resolvase RuvCUniRule annotation
Gene namesi
Name:ruvCUniRule annotation
Ordered Locus Names:FN0214
OrganismiFusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131)
Taxonomic identifieri190304 [NCBI]
Taxonomic lineageiBacteriaFusobacteriaFusobacterialesFusobacteriaceaeFusobacterium
Proteomesi
  • UP000002521 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001830991 – 190Crossover junction endodeoxyribonuclease RuvCAdd BLAST190

Proteomic databases

PRIDEiQ8RGS0.

Interactioni

Protein-protein interaction databases

STRINGi190304.FN0214.

Structurei

3D structure databases

ProteinModelPortaliQ8RGS0.
SMRiQ8RGS0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvC family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105NHU. Bacteria.
COG0817. LUCA.
HOGENOMiHOG000012182.
InParanoidiQ8RGS0.
KOiK01159.
OMAiGLTRCGI.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00034. RuvC. 1 hit.
InterProiView protein in InterPro
IPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
PANTHERiPTHR30194:SF4. PTHR30194:SF4. 1 hit.
PfamiView protein in Pfam
PF02075. RuvC. 1 hit.
PRINTSiPR00696. RSOLVASERUVC.
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00228. ruvC. 1 hit.
PROSITEiView protein in PROSITE
PS01321. RUVC. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8RGS0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVIGIDPGT AIVGYGIIDY DKNKYSIVDY GVVLTSKDLS TEERLEIVYD
60 70 80 90 100
EIDKILKKYK PEFMAIEDLF YFKNNKTVIS VAQARGVILL AGKQNNIAMT
110 120 130 140 150
SYTPLQVKIG ITGYGKAEKK QIQQMVQKFL GLSEIPKPDD AADALAICIT
160 170 180 190
HINSLGSKLS FGRTNNLNKI VVPSGTNKIS LEEYKNLLKK
Length:190
Mass (Da):21,139
Last modified:June 1, 2002 - v1
Checksum:i9CD995FB04CDB564
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009951 Genomic DNA. Translation: AAL94420.1.
RefSeqiNP_603121.1. NC_003454.1.
WP_005902123.1. NC_003454.1.

Genome annotation databases

EnsemblBacteriaiAAL94420; AAL94420; FN0214.
GeneIDi991740.
KEGGifnu:FN0214.
PATRICifig|190304.8.peg.795.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiRUVC_FUSNN
AccessioniPrimary (citable) accession number: Q8RGS0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 2, 2002
Last sequence update: June 1, 2002
Last modified: June 7, 2017
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families