Q8RGS0 (RUVC_FUSNN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Crossover junction endodeoxyribonuclease RuvC
EC=3.1.22.4

Alternative name(s):
Holliday junction nuclease RuvC
Holliday junction resolvase RuvC

Gene names

Name:	ruvC
Ordered Locus Names:	FN0214

Organism

Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131) [Reference proteome] [HAMAP]

Taxonomic identifier

190304 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Fusobacteria › Fusobacteriia › Fusobacteriales › Fusobacteriaceae › Fusobacterium › Fusobacterium nucleatum › Fusobacterium nucleatum subsp. nucleatum

Protein attributes

Sequence length	190 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group By similarity. HAMAP-Rule MF_00034
Catalytic activity	Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction). HAMAP-Rule MF_00034
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Sequence similarities	Belongs to the RuvC family.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA recombination Inferred from electronic annotation. Source: HAMAP DNA repair Inferred from electronic annotation. Source: HAMAP nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	crossover junction endodeoxyribonuclease activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 190	190	Crossover junction endodeoxyribonuclease RuvC HAMAP-Rule MF_00034		PRO_0000183099
Sites
	Metal binding	7	1	Magnesium By similarity
	Metal binding	67	1	Magnesium By similarity
	Metal binding	140	1	Magnesium By similarity
	Metal binding	143	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8RGS0 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 9CD995FB04CDB564	FASTA	190	21,139
	10 20 30 40 50 60 MRVIGIDPGT AIVGYGIIDY DKNKYSIVDY GVVLTSKDLS TEERLEIVYD EIDKILKKYK 70 80 90 100 110 120 PEFMAIEDLF YFKNNKTVIS VAQARGVILL AGKQNNIAMT SYTPLQVKIG ITGYGKAEKK 130 140 150 160 170 180 QIQQMVQKFL GLSEIPKPDD AADALAICIT HINSLGSKLS FGRTNNLNKI VVPSGTNKIS 190 LEEYKNLLKK

References

[1]

"Genome sequence and analysis of the oral bacterium Fusobacterium nucleatum strain ATCC 25586." Kapatral V., Anderson I., Ivanova N., Reznik G., Los T., Lykidis A., Bhattacharyya A., Bartman A., Gardner W., Grechkin G., Zhu L., Vasieva O., Chu L., Kogan Y., Chaga O., Goltsman E., Bernal A., Larsen N. , D'Souza M., Walunas T., Pusch G., Haselkorn R., Fonstein M., Kyrpides N.C., Overbeek R. J. Bacteriol. 184:2005-2018(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009951 Genomic DNA. Translation: AAL94420.1.
RefSeq	NP_603121.1. NC_003454.1.
3D structure databases
ProteinModelPortal	Q8RGS0.
ModBase	Search...
Protein-protein interaction databases
STRING	190304.FN0214.
Proteomic databases
PRIDE	Q8RGS0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL94420; AAL94420; FN0214.
GeneID	991740.
KEGG	fnu:FN0214.
PATRIC	21950205. VBIFusNuc122357_0795.
Phylogenomic databases
eggNOG	COG0817.
HOGENOM	HOG000012182.
KO	K01159.
OMA	YTALQMK.
ProtClustDB	PRK00039.
Family and domain databases
HAMAP	MF_00034. RuvC.
InterPro	IPR012337. RNaseH-like_dom. IPR020563. X-over_junc_endoDNase_Mg_BS. IPR002176. X-over_junc_endoDNase_RuvC. [Graphical view]
Pfam	PF02075. RuvC. 1 hit. [Graphical view]
PRINTS	PR00696. RSOLVASERUVC.
SUPFAM	SSF53098. RNaseH_fold. 1 hit.
TIGRFAMs	TIGR00228. ruvC. 1 hit.
PROSITE	PS01321. RUVC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RUVC_FUSNN

Accession

Primary (citable) accession number: Q8RGS0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 2, 2002
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program