Q8RGH3 (ALF1_FUSNN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fructose-bisphosphate aldolase class 1
EC=4.1.2.13

Alternative name(s):
Fructose-bisphosphate aldolase class I
Short name=FBP aldolase

Gene names

Name:	fda
Ordered Locus Names:	FN0322

Organism

Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131) [Reference proteome] [HAMAP]

Taxonomic identifier

190304 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Fusobacteria › Fusobacteriia › Fusobacteriales › Fusobacteriaceae › Fusobacterium › Fusobacterium nucleatum › Fusobacterium nucleatum subsp. nucleatum

Protein attributes

Sequence length	295 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate. HAMAP-Rule MF_00729
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4. HAMAP-Rule MF_00729
Sequence similarities	Belongs to the class I fructose-bisphosphate aldolase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: HAMAP
Molecular_function	fructose-bisphosphate aldolase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 295	295	Fructose-bisphosphate aldolase class 1 HAMAP-Rule MF_00729		PRO_0000216900
Sites
	Active site	176	1	Proton acceptor By similarity
	Active site	213	1	Schiff-base intermediate with dihydroxyacetone-P By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8RGH3 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 492DA50FBAC26495	FASTA	295	32,896
	10 20 30 40 50 60 MNEKLEKMRN GKGFIAALDQ SGGSTPKALK LYGVNENEYS NDKEMFDLIH KMRTRIIKSP 70 80 90 100 110 120 AFNESKILGA ILFEQTMDSK IDGKYTADFL WEEKKVLPFL KIDKGLNDLD ADGVQTMKPN 130 140 150 160 170 180 PTLADLLKRA NERHIFGTKM RSVIKKASPA GIARVVEQQF EVAAQVVAAG LIPIIEPEVD 190 200 210 220 230 240 INNVDKVQCE EILRDEIRKH LNALPETSNV MLKLTLPTVE NLYEEFTKHP RVVRVVALSG 250 260 270 280 290 GYSREKANDI LSKNKGVIAS FSRALTEGLS AQQTDEEFNK TLAASIDGIY EASVK

References

[1]

"Genome sequence and analysis of the oral bacterium Fusobacterium nucleatum strain ATCC 25586." Kapatral V., Anderson I., Ivanova N., Reznik G., Los T., Lykidis A., Bhattacharyya A., Bartman A., Gardner W., Grechkin G., Zhu L., Vasieva O., Chu L., Kogan Y., Chaga O., Goltsman E., Bernal A., Larsen N. , D'Souza M., Walunas T., Pusch G., Haselkorn R., Fonstein M., Kyrpides N.C., Overbeek R. J. Bacteriol. 184:2005-2018(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009951 Genomic DNA. Translation: AAL94528.1.
RefSeq	NP_603229.1. NC_003454.1.
3D structure databases
ProteinModelPortal	Q8RGH3.
SMR	Q8RGH3. Positions 1-295.
ModBase	Search...
Protein-protein interaction databases
STRING	190304.FN0322.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL94528; AAL94528; FN0322.
GeneID	991677.
KEGG	fnu:FN0322.
PATRIC	21950419. VBIFusNuc122357_0902.
Phylogenomic databases
eggNOG	COG3588.
HOGENOM	HOG000073502.
KO	K01623.
OMA	GTKMRSV.
ProtClustDB	PRK05377.
Enzyme and pathway databases
UniPathway	UPA00109; UER00183.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_00729. FBP_aldolase_1.
InterPro	IPR000741. Aldolase_I. IPR013785. Aldolase_TIM. IPR023014. FBP_aldolase_I_bac. [Graphical view]
Pfam	PF00274. Glycolytic. 2 hits. [Graphical view]
PROSITE	PS00158. ALDOLASE_CLASS_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALF1_FUSNN

Accession

Primary (citable) accession number: Q8RGH3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 21, 2003
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program