Q8RG88 (PYRC_FUSNN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroorotase
Short name=DHOase
EC=3.5.2.3

Gene names

Name:	pyrC
Ordered Locus Names:	FN0420

Organism

Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131)

Taxonomic identifier

190304 [NCBI]

Taxonomic lineage

Bacteria › Fusobacteria › Fusobacterales › Fusobacteriaceae › Fusobacterium

Protein attributes

Sequence length	425 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate. HAMAP MF_00220_B
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_00220_B
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3. HAMAP MF_00220_B
Subunit structure	Homodimer By similarity. HAMAP MF_00220_B
Sequence similarities	Belongs to the DHOase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	dihydroorotase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 425	425	Dihydroorotase HAMAP MF_00220_B		PRO_0000147234
Sites
	Metal binding	56	1	Zinc 1 By similarity
	Metal binding	58	1	Zinc 1 By similarity
	Metal binding	174	1	Zinc 2 By similarity
	Metal binding	227	1	Zinc 2 By similarity
	Metal binding	300	1	Zinc 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8RG88 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 1AA264FD8179C981	FASTA	425	47,382
	10 20 30 40 50 60 MLLKNCKILK NTKFEKVDIL IRDNKIEKIS ENIDITDENI IDIKNRFVTA GFIDVHVHWR 70 80 90 100 110 120 EPGFSKKETV YTASRAAARG GFTTVMTMPN LNPVPDSVET LNKQLEIIKK DSVIRAIPYG 130 140 150 160 170 180 AITKEEYGRE LSDMEAIASN VFAFTDDGRG VQSANVMYEA MLMGAKLNKA IVAHCEDNSL 190 200 210 220 230 240 IRGGAMHEGK RSAELGIKGI PSICESTQIV RDVLLAEAAN CHYHVCHISA KESVRAVREG 250 260 270 280 290 300 KKNGIKVTCE VTPHHLLSCD EDIKEDNGMW KMNPPLRSRE DRNALIVGIL DGTIDIIATD 310 320 330 340 350 360 HAPHTMEEKI RGIEKSSFGI VGSETAFAQL YTKFVKTDIF SLEMLVKLMS ENVAKIFDLP 370 380 390 400 410 420 YGKLEENSFA DIVVIDLEKE ITINPNNFLS KGKNTPYINE KINGIPVLTI SNGKIAYIDK EEINL

References

[1]

"Genome sequence and analysis of the oral bacterium Fusobacterium nucleatum strain ATCC 25586." Kapatral V., Anderson I., Ivanova N., Reznik G., Los T., Lykidis A., Bhattacharyya A., Bartman A., Gardner W., Grechkin G., Zhu L., Vasieva O., Chu L., Kogan Y., Chaga O., Goltsman E., Bernal A., Larsen N. , D'Souza M., Walunas T., Pusch G., Haselkorn R., Fonstein M., Kyrpides N.C., Overbeek R. J. Bacteriol. 184:2005-2018(2002) [PubMed: 11889109] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009951 Genomic DNA. Translation: AAL94623.1.
RefSeq	NP_603324.1. NC_003454.1.
3D structure databases
ProteinModelPortal	Q8RG88.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	992862.
GenomeReviews	Gene locus FN0420 in contig AE009951_GR.
KEGG	fnu:FN0420.
NMPDR	fig|190304.1.peg.1003.
PATRIC	21950615. VBIFusNuc122357_0997.
Phylogenomic databases
HOGENOM	HBG724623.
OMA	PSICEST.
PhylomeDB	Q8RG88.
ProtClustDB	PRK09357.
Enzyme and pathway databases
BioCyc	FNUC190304:FN0420-MONOMER.
Family and domain databases
HAMAP	MF_00220_B. PyrC_type2_B. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR004722. DHOase. IPR002195. Dihydroorotase_CS. IPR011059. Metal-dep_hydrolase_composite. [Graphical view]
KO	K01465.
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
SUPFAM	SSF51338. Metalo_hydrolase. 1 hit.
TIGRFAMs	TIGR00857. PyrC_multi. 1 hit.
PROSITE	PS00482. DIHYDROOROTASE_1. False negative. PS00483. DIHYDROOROTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRC_FUSNN

Accession

Primary (citable) accession number: Q8RG88

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 26, 2002
Last sequence update:	June 1, 2002
Last modified:	January 25, 2012
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program