Reviewed, UniProtKB/Swiss-Prot Q8RG87 (CARA_FUSNN)

Names and origin

Protein names

Recommended name:
    Carbamoyl-phosphate synthase small chain
    EC=6.3.5.5
Alternative name(s):
    Carbamoyl-phosphate synthetase glutamine chain

Gene names

Name:	carA
Ordered Locus Names:	FN0421

Organism

Fusobacterium nucleatum subsp. nucleatum [Complete proteome] [HAMAP]

Taxonomic identifier

76856 [NCBI]

Taxonomic lineage

Bacteria › Fusobacteria › Fusobacterales › Fusobacteriaceae › Fusobacterium

Protein attributes

Sequence length	358 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP MF_01209
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from HCO(3)(-): step 1/1. HAMAP MF_01209 Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 1/6. HAMAP MF_01209
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity.
Sequence similarities	Belongs to the carA family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 358	358	Carbamoyl-phosphate synthase small chain HAMAP MF_01209		PRO_0000112278
Regions
	Domain	172 – 358	187	Glutamine amidotransferase type-1
	Region	1 – 168	168	CPSase HAMAP MF_01209
Sites
	Active site	247	1	Nucleophile By similarity
	Active site	332	1	By similarity
	Active site	334	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8RG87-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: F28A3CBA83E4263D	FASTA	358	39,448
	10 20 30 40 50 60 MYNRQLILED GTVYKGYAFG ADVENVGEVV FNTSMTGYQE ILSDPSYNGQ IVTLTYPLIG 70 80 90 100 110 120 NYGINRDDFE SMKPCIKGMV VKEVCATPSN FRSEKTLDEA LKDFGIPGIY GIDTRALTRK 130 140 150 160 170 180 LRSKGVVKGC LVSIDRNVDE VVAELKKTVL PTNQIEQVSS KSISPALGRG RRVVLVDLGM 190 200 210 220 230 240 KIGIVRELVS RGCDVIVVPY NTTAEEVLRL EPDGVMLTNG PGDPEDAKES IEMIKGIINK 250 260 270 280 290 300 VTIFGICMGH QLVSLACGAK TYKLKFGHRG GNHPVKNILT GRVDITSQNH GYAVDIDSLN 310 320 330 340 350 DTDLELTHIA INDRSCEGVR HKKYPVFTVQ FHPEAAAGPH DTSYLFDEFI KNIDNNMK

References

[1]

"Genome sequence and analysis of the oral bacterium Fusobacterium nucleatum strain ATCC 25586." Kapatral V., Anderson I., Ivanova N., Reznik G., Los T., Lykidis A., Bhattacharyya A., Bartman A., Gardner W., Grechkin G., Zhu L., Vasieva O., Chu L., Kogan Y., Chaga O., Goltsman E., Bernal A., Larsen N. , D'Souza M., Walunas T., Pusch G., Haselkorn R., Fonstein M., Kyrpides N.C., Overbeek R. J. Bacteriol. 184:2005-2018(2002) [PubMed: 11889109] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE009951 Genomic DNA. Translation: AAL94624.1.
RefSeq	NP_603325.1.
3D structure databases
HSSP	HSSP built from PDB template 1A9X based on UniProtKB P00907.
ModBase	Search...
Genome annotation databases
GeneID	991442.
GenomeReviews	Gene locus FN0421 in contig AE009951_GR.
KEGG	fnu:FN0421.
NMPDR	fig|190304.1.peg.1004.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8RG87.
OMA	Q8RG87. LFDGSNC.
Enzyme and pathway databases
BioCyc	FNUC190304:FN0421-MON.
BRENDA	6.3.5.5. 289963.
Family and domain databases
HAMAP	MF_01209. [Tree]
InterPro	IPR006220. Anth_synthII. IPR001317. CarbamoylP_synth_GATase. IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11405:SF4. CarA_synth_small. 1 hit.
Pfam	PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
PRINTS	PR00097. ANTSNTHASEII. PR00099. CPSGATASE. PR00096. GATASE.
TIGRFAMs	TIGR01368. CPSaseIIsmall. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARA_FUSNN

Accession

Primary (citable) accession number: Q8RG87

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2002
Last sequence update:	June 1, 2002
Last modified:	June 16, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)